{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T20:46:13Z","timestamp":1770065173225,"version":"3.49.0"},"reference-count":81,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T00:00:00Z","timestamp":1638576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Background. Sudden unexpected death (SUD) is one of the most important and worthy investigation case profiles in emergency medicine and forensic pathology. Sudden unexpected deaths in adults (SUDA) are frequently caused by cardiac events, while infections usually cause those in infants younger than one year (SUDI), and to a lesser extent, in children older than one year (SUDC). However, in some instances of children under the age of one dying (SIDS), a cause is not discovered despite a thorough investigation that includes a review of clinical history, examination of the death scene, and a complete autopsy. Several studies demonstrate that the microbiome influences host immunity, alters susceptibility to viral respiratory infections, and has a vital role in various health, disease, and death outcomes. The main objective of this systematic review was to compile and offer a complete vision of the main lines of research on microbiome and sudden death that have emerged in recent years and their relationship with forensic sciences, as well as the possible contributions or limitations in the field of forensic sciences. Methods. Following PRISMA principles, a systematic evaluation of the microbiome and sudden death in forensic science was conducted. In this review, our study classified the sudden deaths as SUDA, SUDI, and SIDS. Results. The role of microbiome research in sudden death is discussed in this review. Various studies have linked the detection of different bacteria or viruses as a probable cause of sudden death. Bacteria analysed differ between studies that used autopsy specimens from deaths classified as SUDA, SUDI, and SIDS, or, except in the case of Staphylococcus aureus and Escherichia coli, which have been analysed in both SUDI and SIDS autopsies. In the case of viruses, only Cytomegalovirus has been analysed in both SIDS and SUDI cases. However, all the viruses studied are respiratory viruses found in samples of nasopharyngeal or lung fluid. Conclusions. Although the application of the microbiome in sudden death and other fields of forensic science is still in its early stages, a role of the microbiome in sudden deaths cannot be ruled out, but we cannot conclude that it is a significant factor either.<\/jats:p>","DOI":"10.3390\/life11121345","type":"journal-article","created":{"date-parts":[[2021,12,5]],"date-time":"2021-12-05T21:01:45Z","timestamp":1638738105000},"page":"1345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Is There a Role for the Microbiome and Sudden Death? A Systematic Review"],"prefix":"10.3390","volume":"11","author":[{"given":"Aurelia","family":"Collados-Ros","sequence":"first","affiliation":[{"name":"Department of Legal and Forensic Medicine, Biomedical Research Institute (IMIB), Faculty of Medicine, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia, E-30110 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-2969","authenticated-orcid":false,"given":"Mar\u00eda D.","family":"P\u00e9rez-C\u00e1rceles","sequence":"additional","affiliation":[{"name":"Department of Legal and Forensic Medicine, Biomedical Research Institute (IMIB), Faculty of Medicine, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia, E-30110 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1140-4313","authenticated-orcid":false,"given":"Isabel","family":"Legaz","sequence":"additional","affiliation":[{"name":"Department of Legal and Forensic Medicine, Biomedical Research Institute (IMIB), Faculty of Medicine, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia, E-30110 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Madea, B., and Argo, A. (2014). Certification of Death: External Postmortem Examination. Handbook of Forensic Medicine, John Wiley & Sons, Ltd.","DOI":"10.1002\/9781118570654.ch6"},{"key":"ref_2","unstructured":"World Health Organization (2021, November 20). International Classification of Diseases (ICD-10). Available online: https:\/\/icd.who.int\/browse10\/2019\/en#\/R96.0."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1177\/0025802417737001","article-title":"Gastrointestinal causes of sudden unexpected death: A review","volume":"58","author":"Menezes","year":"2018","journal-title":"Med. Sci. Law"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1177\/0025802413491246","article-title":"Study of sudden gastrointestinal deaths: An autopsy study","volume":"54","author":"Hugar","year":"2014","journal-title":"Med. Sci. Law"},{"key":"ref_5","first-page":"2","article-title":"Epidemiology of Sudden Cardiac Death","volume":"13","author":"Brugada","year":"2013","journal-title":"Rev. Espa\u00f1ola Cardiol. Supl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1054\/cdip.2000.0050","article-title":"Sudden unexpected death in infancy","volume":"7","author":"Gould","year":"2001","journal-title":"Curr. Diagnostic Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.forsciint.2018.05.023","article-title":"A systematic review exploring the relationship between infection and sudden unexpected death between 2000 and 2016: A forensic perspective","volume":"289","author":"Kruger","year":"2018","journal-title":"Forensic Sci. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"677","DOI":"10.3109\/15513819109065465","article-title":"Defining the sudden infant death syndrome (sids): Deliberations of an expert panel convened by the national institute of child health and human development","volume":"11","author":"Willinger","year":"1991","journal-title":"Fetal Pediatr. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1136\/archdischild-2016-312327","article-title":"Infection: The neglected paradigm in SIDS research","volume":"102","author":"Goldwater","year":"2017","journal-title":"Arch. Dis. Child."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1542\/peds.2005-1499","article-title":"The changing concept of sudden infant death syndrome: Diagnostic coding shifts, controversies regarding the sleeping environment, and new variables to consider in reducing risk","volume":"116","author":"Kattwinkel","year":"2005","journal-title":"Pediatrics"},{"key":"ref_11","first-page":"222","article-title":"Sudden infant death syndrome: Do the parents follow the recommendations?","volume":"92","year":"2020","journal-title":"An. Pediatr."},{"key":"ref_12","unstructured":"(2020). Canadian Agency for Drugs and Technologies in Health Interventions for the Prevention of Sudden Infant Death Syndrome: Guidelines, Canadian Agency for Drugs and Technologies in Health."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Duncan, J.R., and Byard, R.W. (2018). Sudden Unexplained Death in Childhood: An Overview. SIDS Sudden infant and early childhood death: The past, the present and the future, University of Adelaide Press.","DOI":"10.20851\/sids"},{"key":"ref_14","unstructured":"Molina, P., Bermejo, M., Cardona, B., and Giner, S. (2013). Clasificaci\u00f3n de San Diego y Nuevas Tendencias del S\u00edndrome de Muerte S\u00fabita del Lactante, Asociaci\u00f3n Espa\u00f1ola de Pediatr\u00eda."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.mehy.2020.110114","article-title":"SIDS, prone sleep position and infection: An overlooked epidemiological link in current SIDS research? Key evidence for the \u201cInfection Hypothesis.\u201d","volume":"144","author":"Goldwater","year":"2020","journal-title":"Med. Hypotheses"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.ijporl.2011.12.008","article-title":"Otolaryngological aspects of sudden infant death syndrome","volume":"76","author":"Marom","year":"2012","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S0002-9343(99)00354-X","article-title":"Sudden infant death syndrome: Can gastroesophageal reflux cause sudden infant death?","volume":"108","author":"Thach","year":"2000","journal-title":"Am. J. Med."},{"key":"ref_18","first-page":"67","article-title":"Inflammatory responses in sudden infant death syndrome - Past and present views","volume":"25","author":"Vege","year":"1999","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"284","DOI":"10.3928\/19382359-20170721-01","article-title":"Beyond \u201cback to sleep\u201d: Ways to further reduce the risk of sudden infant death syndrome","volume":"46","author":"Hauck","year":"2017","journal-title":"Pediatr. Ann."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.forsciint.2010.07.008","article-title":"Genetics of the sudden infant death syndrome","volume":"203","author":"Courts","year":"2010","journal-title":"Forensic Sci. Int."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Duncan, J.R., and Byard, R.W. (2018). Shared Sleeping Surfaces and Dangerous Sleeping Environments. SIDS Sudden Infant and Early Childhood Death: The Past, the Present and the Future, University of Adelaide Press.","DOI":"10.20851\/sids"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1159\/000244052","article-title":"A Perspective on Neuropathologic Findings in Victims of the Sudden Infant Death Syndrome: The Triple-Risk Model","volume":"65","author":"Filiano","year":"1994","journal-title":"Biol Neonate"},{"key":"ref_23","first-page":"1","article-title":"Impact of the Human Microbiome in Forensic Sciences: a Systematic Review","volume":"86","author":"Osuna","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1001\/jamapediatrics.2016.3345","article-title":"Risk factors, protective factors, and current recommendations to reduce sudden infant death syndrome a review","volume":"171","author":"Carlin","year":"2017","journal-title":"JAMA Pediatr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"10377","DOI":"10.1007\/s00253-018-9414-6","article-title":"Microbial forensics: New breakthroughs and future prospects","volume":"102","author":"Oliveira","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/imj.14248","article-title":"Sudden infant death syndrome: Current perspectives","volume":"49","author":"Horne","year":"2019","journal-title":"Intern. Med. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4070\/kcj.2010.40.6.253","article-title":"Sudden death in pediatric populations","volume":"40","author":"Wu","year":"2010","journal-title":"Korean Circ. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.earlhumdev.2007.07.011","article-title":"Sudden Infant Death Syndrome and parental smoking","volume":"83","author":"Fleming","year":"2007","journal-title":"Early Hum. Dev."},{"key":"ref_29","first-page":"1","article-title":"Deaths: Leading causes for 2014","volume":"65","author":"Heron","year":"2016","journal-title":"Natl. Vital Stat. Reports"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/s00431-011-1433-6","article-title":"Postnatal parental smoking: An important risk factor for SIDS","volume":"170","author":"Lao","year":"2011","journal-title":"Eur. J. Pediatr."},{"key":"ref_31","first-page":"1578","article-title":"Sudden Infant Death Syndrome","volume":"370","author":"Moon","year":"2007","journal-title":"Encycl. Respir. Med. Four-Volume Set"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jpeds.2010.01.027","article-title":"Qualitative Analysis of Beliefs and Perceptions about Sudden Infant Death Syndrome in African-American Mothers: Implications for Safe Sleep Recommendations","volume":"157","author":"Moon","year":"2010","journal-title":"J. Pediatr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Stiffler, D., Ayres, B., Fauvergue, C., and Cullen, D. (2018). Sudden infant death and sleep practices in the Black community. J. Spec. Pediatr. Nurs., 23.","DOI":"10.1111\/jspn.12213"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Duncan, J.R., and Byard, R.W. (2018). Autonomic Cardiorespiratory Physiology and Arousal of the Fetus and Infant. SIDS Sudden Infant and Early Childhood Death: The Past, the Present and the Future, University of Adelaide Press.","DOI":"10.20851\/sids"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1016\/j.cell.2012.01.035","article-title":"Review The Impact of the Gut Microbiota on Human Health: An Integrative View","volume":"148","author":"Clemente","year":"2012","journal-title":"Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/nrgastro.2012.144","article-title":"Microbial contact during pregnancy, intestinal colonization and human disease","volume":"9","author":"Rautava","year":"2012","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mib.2016.10.003","article-title":"Microbiome, metabolites and host immunity","volume":"35","author":"Levy","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1093\/cid\/ciz968","article-title":"Association between the respiratory microbiome and susceptibility to influenza virus infection","volume":"71","author":"Tsang","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jaci.2013.08.020","article-title":"Prebiotic and probiotic supplementation prevents rhinovirus infections in preterm infants: A randomized, placebo-controlled trial","volume":"133","author":"Luoto","year":"2014","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5354","DOI":"10.1073\/pnas.1019378108","article-title":"Microbiota regulates immune defense against respiratory tract influenza a virus infection","volume":"108","author":"Ichinohe","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nature23480","article-title":"A randomized synbiotic trial to prevent sepsis among infants in rural India","volume":"548","author":"Panigrahi","year":"2017","journal-title":"Nature"},{"key":"ref_42","first-page":"158","article-title":"Commensal Bacteria Calibrate the Activation Threshold of Innate Antiviral Immunity","volume":"27","author":"Abt","year":"2012","journal-title":"Inmunity"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature11552","article-title":"Functional interactions between the gut microbiota and host metabolism","volume":"489","author":"Tremaroli","year":"2012","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1001\/jama.2015.3656","article-title":"Preferred reporting items for a systematic review and meta-analysis of individual participant data: The PRISMA-IPD statement","volume":"313","author":"Stewart","year":"2015","journal-title":"J. Am. Med. Assoc."},{"key":"ref_45","unstructured":"Green, S. (2011). Cochrane Handbook for Systematic Reviews of Interventions, The Cochrane Collaboration. version 5.1.0."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1093\/pubmed\/fdi056","article-title":"It\u2019s in your hands: The value of handsearching in conducting systematic reviews of public health interventions","volume":"27","author":"Armstrong","year":"2005","journal-title":"J. Public Health (Bangkok)."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jclinepi.2016.12.007","article-title":"Specific agreement on dichotomous outcomes can be calculated for more than two raters","volume":"83","author":"Dikmans","year":"2017","journal-title":"J. Clin. Epidemiol."},{"key":"ref_48","unstructured":"Critical Appraisal Skills Programme (2020). CASP Checklists, Critical Apraisal Skills Programme."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1136\/bmj.328.7454.1490","article-title":"Grading quality of evidence and strength of recommendations","volume":"328","author":"Oxman","year":"2004","journal-title":"Br. Med. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0221345","article-title":"Age-dependent association of gut bacteria with coronary atherosclerosis: Tampere sudden death study","volume":"14","author":"Tuomisto","year":"2019","journal-title":"PLoS One"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1136\/adc.2009.162792","article-title":"Contribution of bacteriology and virology in sudden unexpected death in infancy","volume":"95","author":"Prtak","year":"2010","journal-title":"Arch. Dis. Child."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.forsciint.2014.03.007","article-title":"Profiling the approach to the investigation of viral infections in cases of sudden unexpected death in infancy in the Western Cape Province, South Africa","volume":"239","author":"Burger","year":"2014","journal-title":"Forensic Sci. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1016\/S0140-6736(08)60798-9","article-title":"Infection and sudden unexpected death in infancy: A systematic retrospective case review","volume":"371","author":"Weber","year":"2008","journal-title":"Lancet"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s12024-010-9181-x","article-title":"Virological investigations in sudden unexpected deaths in infancy (SUDI)","volume":"6","author":"Weber","year":"2010","journal-title":"Forensic Sci. Med. Pathol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12024-010-9199-0","article-title":"Staphylococcal toxins in sudden unexpected death in infancy: Experience from a single specialist centre","volume":"7","author":"Weber","year":"2011","journal-title":"Forensic Sci. Med. Pathol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1093\/cid\/cis870","article-title":"Near-universal prevalence of pneumocystis and associated increase in mucus in the lungs of infants with sudden unexpected death","volume":"56","author":"Vargas","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jflm.2015.11.008","article-title":"Postmortem diagnosis of cytomegalovirus and accompanying other infection agents by real-time PCR in cases of sudden unexpected death in infancy (SUDI)","volume":"38","author":"Yagmur","year":"2015","journal-title":"J. Forensic Leg. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.forsciint.2008.02.007","article-title":"Detection of human herpesvirus-6, Epstein-Barr virus and cytomegalovirus in formalin-fixed tissues from sudden infant death: A study with quantitative real-time PCR","volume":"178","author":"Aguilera","year":"2008","journal-title":"Forensic Sci. Int."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1203\/PDR.0b013e31818095f7","article-title":"Helicobacter pylori antigen in stool is associated with SIDS and sudden infant deaths due to infectious disease","volume":"64","author":"Vege","year":"2008","journal-title":"Pediatr. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/j.1365-2672.2009.04473.x","article-title":"Serotypes of Escherichia coli in Sudden Infant Death Syndrome","volume":"108","author":"Pearce","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1574-695X.2009.00592.x","article-title":"Staphylococcal enterotoxin genes are common in Staphylococcus aureus intestinal flora in Sudden infant death syndrome (SIDS) and live comparison infants","volume":"57","author":"Highet","year":"2009","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.ijmm.2014.05.007","article-title":"Gut microbiome in sudden infant death syndrome (SIDS) differs from that in healthy comparison babies and offers an explanation for the risk factor of prone position","volume":"304","author":"Highet","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.forsciint.2016.03.002","article-title":"Postmortem diagnosis of infectious heart diseases: A mystifying cause of Sudden Infant Death","volume":"262","author":"Gaaloul","year":"2017","journal-title":"Forensic Sci. Int."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jpeds.2017.08.070","article-title":"Intestinal Microbiota Composition in Sudden Infant Death Syndrome and Age-Matched Controls","volume":"191","author":"Leong","year":"2017","journal-title":"J. Pediatr."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Al Mana, H., Yassine, H.M., Younes, N.N., Al-Mohannadi, A., Al-Sadeq, D.W., Alhababi, D., Nasser, E.A., and Nasrallah, G.K. (2019). The current status of cytomegalovirus (CMV) prevalence in the MENA region: A systematic review. Pathogens, 8.","DOI":"10.3390\/pathogens8040213"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.femsim.2004.06.019","article-title":"Mucosal immune responses to infections in infants with acute life threatening events classified as \u201cnear-miss\u201d sudden infant death syndrome","volume":"42","author":"Gleeson","year":"2004","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Aagaard, K., Ma, J., Antony, K.M., Ganu, R., Petrosino, J., and Versalovic, J. (2014). The placenta harbors a unique microbiome. Sci. Transl. Med., 6.","DOI":"10.1126\/scitranslmed.3008599"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0003056","article-title":"Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: A molecular and culture-based investigation","volume":"3","author":"DiGiulio","year":"2008","journal-title":"PLoS One"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1007\/s00284-005-0020-3","article-title":"Isolation of commensal bacteria from umbilical cord blood of healthy neonates born by cesarean section","volume":"51","author":"Odriozola","year":"2005","journal-title":"Curr. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40168-016-0172-3","article-title":"Comparison of placenta samples with contamination controls does not provide evidence for a distinct placenta microbiota","volume":"4","author":"Lauder","year":"2016","journal-title":"Microbiome"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12915-014-0087-z","article-title":"Reagent and laboratory contamination can critically impact sequence-based microbiome analyses","volume":"12","author":"Salter","year":"2014","journal-title":"BMC Biol."},{"key":"ref_72","first-page":"1","article-title":"A critical assessment of the \u201csterile womb\u201d and \u201cin utero colonization\u201d hypotheses: Implications for research on the pioneer infant microbiome","volume":"5","author":"Arrieta","year":"2017","journal-title":"Microbiome"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0723-2020(98)80009-X","article-title":"Design and evaluation of a 16S rRNA-Targeted oligonucleotide probe for specific detection and quantitation of human faecal Bacteroides populations","volume":"21","author":"Schir","year":"1998","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/MMBR.00036-17","article-title":"The First Microbial Colonizers of the Human Gut: Composition, Activities, and Health Implications of the Infant Gut Microbiota","volume":"81","author":"Milani","year":"2017","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_75","first-page":"61","article-title":"Analysis of Intestinal Flora Development in Breast-Fed and Formula-Fed Infants by Using Molecular Identification and Detection Methods","volume":"30","author":"Harmsen","year":"2000","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1099\/00222615-15-2-189","article-title":"The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life","volume":"15","author":"Stark","year":"1982","journal-title":"J. Med. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40168-018-0490-8","article-title":"Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner","volume":"6","author":"Lundgren","year":"2018","journal-title":"Microbiome"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.anaerobe.2011.03.009","article-title":"Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH)","volume":"17","author":"Bezirtzoglou","year":"2011","journal-title":"Anaerobe"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2015.00269","article-title":"Gut microbiota and immunity: Possible role in sudden infant death syndrome","volume":"6","author":"Goldwater","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1007\/s10096-017-2943-6","article-title":"How is post-mortem microbiology appraised by pathologists? Results from a practice survey conducted by ESGFOR","volume":"36","author":"Saegeman","year":"2017","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.eimc.2012.09.019","article-title":"An\u00e1lisis microbiol\u00f3gico post m\u00f3rtem","volume":"31","author":"Alberola","year":"2013","journal-title":"Enferm. Infecc. Y Microbiol. 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