{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T13:20:19Z","timestamp":1779196819446,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T00:00:00Z","timestamp":1639094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Understanding whether SARS-CoV-2 could infect cells and tissues handled during ART is crucial for risk mitigation, especially during the implantation window when either endometrial biopsies are often practiced for endometrial receptivity assessment or embryo transfer is performed. To address this question, this review analyzed current knowledge of the field and retrospectively examined the gene expression profiles of SARS-CoV-2-associated receptors and proteases in a cohort of ART candidates using our previous Affymetrix microarray data. Human endometrial tissue under natural and controlled ovarian stimulation cycles and preimplantation embryos were analyzed. A focus was particularly drawn on the renin-angiotensin system, which plays a prominent role in the virus infection, and we compared the gene expression levels of receptors and proteases related to SARS-CoV-2 infection in the samples. High prevalence of genes related to the ACE2 pathway during both cycle phases and mainly during the mid-secretory phase for ACE2 were reported. The impact of COS protocols on endometrial gene expression profile of SARS-CoV-2-associated receptors and proteases is minimal, suggesting no additional potential risks during stimulated ART procedure. In blastocysts, ACE2, BSG, CTSL, CTSA and FURIN were detectable in the entire cohort at high expression level. Specimens from female genital tract should be considered as potential targets for SARS-CoV-2, especially during the implantation window.<\/jats:p>","DOI":"10.3390\/life11121378","type":"journal-article","created":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T08:17:58Z","timestamp":1639124278000},"page":"1378","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-8404","authenticated-orcid":false,"given":"Delphine","family":"Haouzi","sequence":"first","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"IRMB (Institute for Regenerative Medicine & Biotherapy), Univ Montpellier, INSERM, 34295 Montpellier, France"},{"name":"CHU Montpellier, ART\/PGD Department, Arnaud de Villeneuve Hospital, 34295 Montpellier, France"},{"name":"Global ART Innovation Network, IRMB, CHU Montpellier, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frida","family":"Entezami","sequence":"additional","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"ART Department, American Hospital of Paris, 92200 Neuilly-Sur-Seine, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5866-9530","authenticated-orcid":false,"given":"Edward","family":"Tuaillon","sequence":"additional","affiliation":[{"name":"CHU Montpellier, Bacteriology-Virology Department, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Gala","sequence":"additional","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"CHU Montpellier, ART\/PGD Department, Arnaud de Villeneuve Hospital, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Ferri\u00e8res-Hoa","sequence":"additional","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"CHU Montpellier, ART\/PGD Department, Arnaud de Villeneuve Hospital, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Brouillet","sequence":"additional","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"IRMB (Institute for Regenerative Medicine & Biotherapy), Univ Montpellier, INSERM, 34295 Montpellier, France"},{"name":"CHU Montpellier, ART\/PGD Department, Arnaud de Villeneuve Hospital, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain R.","family":"Thierry","sequence":"additional","affiliation":[{"name":"Regional Institute of Cancer of Montpellier, 34090 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samir","family":"Hamamah","sequence":"additional","affiliation":[{"name":"Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, France"},{"name":"IRMB (Institute for Regenerative Medicine & Biotherapy), Univ Montpellier, INSERM, 34295 Montpellier, France"},{"name":"CHU Montpellier, ART\/PGD Department, Arnaud de Villeneuve Hospital, 34295 Montpellier, France"},{"name":"Global ART Innovation Network, IRMB, CHU Montpellier, 34295 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4383","DOI":"10.1007\/s11033-020-05478-4","article-title":"Expressions and significances of the angiotensin-converting enzyme 2 gene, the receptor of SARS-CoV-2 for COVID-19","volume":"47","author":"Fu","year":"2020","journal-title":"Mol. 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