{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:22:29Z","timestamp":1790882549468,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006704","name":"University of Ostrava","doi-asserted-by":"publisher","award":["SGS09\/P\u0159F\/2019"],"award-info":[{"award-number":["SGS09\/P\u0159F\/2019"]}],"id":[{"id":"10.13039\/501100006704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006704","name":"University of Ostrava","doi-asserted-by":"publisher","award":["SGS01\/P\u0159F\/2020"],"award-info":[{"award-number":["SGS01\/P\u0159F\/2020"]}],"id":[{"id":"10.13039\/501100006704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006704","name":"University of Ostrava","doi-asserted-by":"publisher","award":["SGS11\/P\u0159F\/2021"],"award-info":[{"award-number":["SGS11\/P\u0159F\/2021"]}],"id":[{"id":"10.13039\/501100006704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016287","name":"Center for Geosphere Dynamics","doi-asserted-by":"publisher","award":["UNCE\/SCI\/006"],"award-info":[{"award-number":["UNCE\/SCI\/006"]}],"id":[{"id":"10.13039\/100016287","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Operational Programme Research, Development and Education","award":["CZ.02.1.01\/0.0\/0.0\/17\\_049\/0008441"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17\\_049\/0008441"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Wastewater is considered the most serious source of the spread of antibiotic resistance in the environment. This work, therefore, focuses on the fate and spread of antibiotic resistance genes (ARGs) in wastewater and the monitoring of multidrug-resistant strains. ARGs were monitored in the nitrification and sedimentation tanks of the wastewater treatment plant (WWTP) and in the dam into which this WWTP flows, at various times. The highest relative abundance was found for the blaTEM &gt; tetW &gt; blaNDM-1 &gt; vanA resistance genes, respectively. An increased concentration of tetracycline (up to 96.00 ng\/L) and ampicillin (up to 19.00 ng\/L) was found in water samples compared to other antibiotics detected. The increased incidence of seven ARGs and four antibiotics was observed in the November and December sampling times. Isolated ampicillin-resistant strains showed a high degree of resistance to ampicillin (61.2% of the total isolates had a minimum inhibitory concentration (MIC) \u2265 20 mg\/mL). In 87.8% of isolates, out of the total number, the occurrence of two or more ARGs was confirmed. These multidrug-resistant strains were most often identified as Aeromonas sp. This strain could represent a significant role in the spread of multidrug resistance through wastewater in the environment.<\/jats:p>","DOI":"10.3390\/life12020147","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T22:51:06Z","timestamp":1642719066000},"page":"147","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Resistant Genes and Multidrug-Resistant Bacteria in Wastewater: A Study of Their Transfer to the Water Reservoir in the Czech Republic"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0283-6595","authenticated-orcid":false,"given":"Tereza","family":"Stachurov\u00e1","sequence":"first","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikola","family":"S\u00fdkorov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6470-183X","authenticated-orcid":false,"given":"Jaroslav","family":"Semer\u00e1d","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, CZ-142 20 Prague, Czech Republic"},{"name":"Institute for Environmental Studies, Faculty of Science, Charles University, Ben\u00e1tsk\u00e1 2, CZ-128 01 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9997-4881","authenticated-orcid":false,"given":"Kate\u0159ina","family":"Malachov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609459","DOI":"10.3389\/fmicb.2021.609459","article-title":"Futuristic Non-antibiotic Therapies to Combat Antibiotic Resistance: A Review","volume":"12","author":"Kumar","year":"2021","journal-title":"Front. 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