{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:27:15Z","timestamp":1768458435985,"version":"3.49.0"},"reference-count":17,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["1997\/19"],"award-info":[{"award-number":["1997\/19"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>We recently identified a FOXL1+ intestinal subepithelial network of telocytes (TCs) without which epithelial stem and progenitor cells cannot proliferate and support regeneration. In addition to FOXL1 lineage cell distribution along the intestinal epithelium, we also observed their presence within the muscle layers. Here, we characterized FOXL1+ lineage cells along the muscle layers of the duodenum in order to understand their progeny and relation to interstitial Cajal cells (ICCs), smooth muscle cells (SMCs) and the previously reported PDGFRa+ TCs. Using a FOXL1-Cre transgenic line in conjunction with genetic lineage labeling using the Rosa26-mTmG allele, in which Cre-marked cells produce a membrane-targeted version of green fluorescent protein (GFP), we found that within the muscle layers FOXL1 lineage GFP+ cells had two main progeny; (i) elongated multinucleated SMA+ SMCs, intermingled in parallel or perpendicular to muscle fibers. (ii) TCs displaying small cell body with multiple cell processes, expressing PDGFRa and CD34. These findings may suggest a mutual origin for TCs and SMCs.<\/jats:p>","DOI":"10.3390\/life12020176","type":"journal-article","created":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T03:33:32Z","timestamp":1643168012000},"page":"176","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lineage Tracing of FOXL1+ Cells in the Tunica Muscularis Suggests Mutual Origin for Telocytes and Smooth Muscle Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Moriya","family":"Shushan","sequence":"first","affiliation":[{"name":"Department of Developmental Biology and Cancer Research, The Hebrew University, Jerusalem 91120, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Shoshkes-Carmel","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology and Cancer Research, The Hebrew University, Jerusalem 91120, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jcmgh.2015.12.004","article-title":"Foxl1-Expressing Mesenchymal Cells Constitute the Intestinal Stem Cell Niche","volume":"2","author":"Aoki","year":"2015","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/s41586-018-0084-4","article-title":"Subepithelial telocytes are an important source of Wnts that supports intestinal crypts","volume":"557","author":"Wang","year":"2018","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1038\/s41467-020-15714-x","article-title":"Lgr5+ telocytes are a signaling source at the intestinal villus tip","volume":"11","author":"Halpern","year":"2020","journal-title":"Nat Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/dvg.20335","article-title":"A global double-fluorescent Cre reporter mouse","volume":"45","author":"Muzumdar","year":"2007","journal-title":"Genesis"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"E506","DOI":"10.1073\/pnas.1620059114","article-title":"CD34+ mesenchymal cells are a major component of the intestinal stem cells niche at homeostasis and after injury","volume":"114","author":"Stzepourginski","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"E3173","DOI":"10.1073\/pnas.1713510115","article-title":"PDGFR\u03b1+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo","volume":"115","author":"Greicius","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/s41586-018-0190-3","article-title":"GLI1-expressing mesenchymal cells form the essential Wnt-secreting niche for colon stem cells","volume":"558","author":"Degirmenci","year":"2018","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, X., and Cretoiu, D. (2016). A Tale of Two Cells: Telocyte and stem cell unique relationship. Telocytes: Connecting Cells, Springer.","DOI":"10.1007\/978-981-10-1061-3"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wang, X., and Cretoiu, D. (2016). The telocyte subtypes. Telocytes: Connecting Cells, Springer.","DOI":"10.1007\/978-981-10-1061-3"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1111\/jcmm.12134","article-title":"Telocytes express PDGFR\u03b1 in the human gastrointestinal tract","volume":"17","author":"Vannucchi","year":"2013","journal-title":"J. Cell Mol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1111\/j.1582-4934.2010.01059.x","article-title":"Telocytes\u2014A  case of serendipity: The winding way from interstitial cells of Cajal (ICC), via interstitial Cajal-like Cells (ICLC) to telocytes","volume":"14","author":"Popescu","year":"2010","journal-title":"J. Cell Mol. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/j.1582-4934.2011.01465.x","article-title":"Tracheal telocytes","volume":"16","author":"Rusu","year":"2012","journal-title":"J. Cell Mol. 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PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0162858"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/2\/176\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:07:45Z","timestamp":1760134065000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/2\/176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,26]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["life12020176"],"URL":"https:\/\/doi.org\/10.3390\/life12020176","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,26]]}}}