{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:26:20Z","timestamp":1780442780020,"version":"3.54.1"},"reference-count":68,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>One of the most important challenges for soil science is to determine the limits for the sustainable functioning of contaminated ecosystems. The response of soil microbiomes to kerosene pollution is still poorly understood. Here, we model the impact of kerosene leakage on the composition of the topsoil microbiome in pot and field experiments with different loads of added kerosene (loads up to 100 g\/kg; retention time up to 360 days). At four time points we measured kerosene concentration and sequenced variable regions of 16S ribosomal RNA in the microbial communities. Mainly alkaline Dystric Arenosols with low content of available phosphorus and soil organic matter had an increased fraction of Actinobacteriota, Firmicutes, Nitrospirota, Planctomycetota, and, to a lesser extent, Acidobacteriota and Verrucomicobacteriota. In contrast, in highly acidic Fibric Histosols, rich in soil organic matter and available phosphorus, the fraction of Acidobacteriota was higher, while the fraction of Actinobacteriota was lower. Albic Luvisols occupied an intermediate position in terms of both physicochemical properties and microbiome composition. The microbiomes of different soils show similar response to equal kerosene loads. In highly contaminated soils, the proportion of anaerobic bacteria-metabolizing hydrocarbons increased, whereas the proportion of aerobic bacteria decreased. During the field experiment, the soil microbiome recovered much faster than in the pot experiments, possibly due to migration of microorganisms from the polluted area. The microbial community of Fibric Histosols recovered in 6 months after kerosene had been loaded, while microbiomes of Dystric Arenosols and Albic Luvisols did not restore even after a year.<\/jats:p>","DOI":"10.3390\/life12020221","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T08:20:29Z","timestamp":1643617229000},"page":"221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Influence of Kerosene on Microbiomes of Diverse Soils"],"prefix":"10.3390","volume":"12","author":[{"given":"Pavel V.","family":"Shelyakin","sequence":"first","affiliation":[{"name":"Institute for Information Transmission Problems (Kharkevich Institute), Russian Academy of Sciences, 127051 Moscow, Russia"},{"name":"Department of Computational Biology, N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119333 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4309-2809","authenticated-orcid":false,"given":"Ivan N.","family":"Semenkov","sequence":"additional","affiliation":[{"name":"Faculty of Geography, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria N.","family":"Tutukina","sequence":"additional","affiliation":[{"name":"Institute for Information Transmission Problems (Kharkevich Institute), Russian Academy of Sciences, 127051 Moscow, Russia"},{"name":"Center of Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"},{"name":"Lab of Functional Genomics and Cellular Stress, Institute of Cell Biophysics RAS, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria D.","family":"Nikolaeva","sequence":"additional","affiliation":[{"name":"Institute for Information Transmission Problems (Kharkevich Institute), Russian Academy of Sciences, 127051 Moscow, Russia"},{"name":"Center of Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna V.","family":"Sharapova","sequence":"additional","affiliation":[{"name":"Faculty of Geography, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia V.","family":"Sarana","sequence":"additional","affiliation":[{"name":"Center of Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey A.","family":"Lednev","sequence":"additional","affiliation":[{"name":"Faculty of Geography, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander D.","family":"Smolenkov","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail S.","family":"Gelfand","sequence":"additional","affiliation":[{"name":"Institute for Information Transmission Problems (Kharkevich Institute), Russian Academy of Sciences, 127051 Moscow, Russia"},{"name":"Center of Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel P.","family":"Krechetov","sequence":"additional","affiliation":[{"name":"Faculty of Geography, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-2525","authenticated-orcid":false,"given":"Tatiana V.","family":"Koroleva","sequence":"additional","affiliation":[{"name":"Faculty of Geography, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jes.2016.08.022","article-title":"High-throughput metagenomic analysis of petroleum-contaminated soil microbiome reveals the versatility in xenobiotic aromatics metabolism","volume":"56","author":"Bao","year":"2017","journal-title":"J. Environ. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"242","DOI":"10.14430\/arctic2656","article-title":"Effects of Crude and Diesel Oil Spills on Plant Communities at Prudhoe Bay, Alaska, and the Derivation of Oil Spill Sensitivity Maps","volume":"31","author":"Walker","year":"1978","journal-title":"Arctic"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1139\/b78-294","article-title":"Effects of experimental crude oil spills on subarctic boreal forest vegetation near Norman Wells, N.W.T., Canada","volume":"56","author":"Hutchinson","year":"1978","journal-title":"Can. J. Bot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1139\/b94-143","article-title":"Long-term recovery of vegetation on two experimental crude oil spills in interior Alaska black spruce taiga","volume":"72","author":"Racine","year":"1994","journal-title":"Can. J. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"490","DOI":"10.2307\/1551415","article-title":"The effects of crude and diesel oil spills on plant communities at Mesters Vig, northeast Greenland","volume":"19","author":"Holt","year":"1987","journal-title":"Arct. Alp. Res."},{"key":"ref_6","unstructured":"Bay, C. (1997). Effects of Experimental Spills of Crude and Diesel Oil on Arctic Vegetation. A Long-Term Study on High Arctic Terrestrial Plant Communities in Jameson Land, Central East Greenland, NERI Technical Report."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115711","DOI":"10.1016\/j.envpol.2020.115711","article-title":"Ecological consequences of space rocket accidents in Kazakhstan between 1999 and 2018","volume":"268","author":"Koroleva","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1038\/ismej.2017.129","article-title":"Chemical dispersants enhance the activity of oil- and gas condensate-degrading marine bacteria","volume":"11","author":"Tremblay","year":"2017","journal-title":"ISME J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1080\/15320383.2015.1054555","article-title":"Ex-Situ Studies on Biodegradation of Artificially Enriched Kerosene and Diesel Soils by Fungal Isolates","volume":"24","author":"Khan","year":"2015","journal-title":"Soil Sediment Contam."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1080\/10934529.2013.761476","article-title":"Degradation potential and microbial community structure of heavy oil-enriched microbial consortia from mangrove sediments in Okinawa, Japan","volume":"48","author":"Bacosa","year":"2013","journal-title":"J. Environ. Sci. Health Part A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.3389\/fmicb.2018.02885","article-title":"Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis","volume":"9","author":"Xu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_12","first-page":"52","article-title":"Soil plant microbe interactions in phytoremediation","volume":"78","author":"Karthikeyan","year":"2003","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1007\/s00128-014-1358-7","article-title":"Effects of diesel and kerosene on germination and growth of coastal wetland plant species","volume":"93","author":"Kim","year":"2014","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mackenzie, J.S., and Jeggo, M. (2019). The one health approach-why is it so important?. Trop. Med. Infect. Dis., 4.","DOI":"10.3390\/tropicalmed4020088"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.trd.2017.10.013","article-title":"The environmental impact of space transport","volume":"58","author":"Koroleva","year":"2018","journal-title":"Transp. Res. Part D Transp. Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.ecolind.2019.01.045","article-title":"The natural regeneration of desert ecosystem vegetation at the 2013 crash site of a Proton-M launch vehicle, Republic of Kazakhstan","volume":"101","author":"Lednev","year":"2019","journal-title":"Ecol. Indic."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1127\/0941-2948\/2006\/0130","article-title":"World Maps of K\u00f6ppen-Geiger climate classification updated","volume":"15","author":"Kottek","year":"2006","journal-title":"Meteorol. Zeitschrift."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"144965","DOI":"10.1016\/j.scitotenv.2021.144965","article-title":"Impact of kerosene pollution on ground vegetation of southern taiga in the Amur Region, Russia","volume":"772","author":"Lednev","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_19","unstructured":"Dorokhova, M.F., Krechetov, P.P., Koroleva, T.V., and Sharapova, A.V. (2015, January 19\u201323). Algo-cyanobacterial communities as indicators of soil pollution with jet-fuel. Algae Cyanobacteria Nat. Agric. Ecosyst. Proceedings of the II Int. Sci. Pract. Conf. Dedic. to 105th Anniv. Birth Profr. Emilia Adrianovna Shtina, Kirov, Russia."},{"key":"ref_20","first-page":"233","article-title":"The Effect of Kerosene Pollution on the Cellulolytic Activity of Albic Retisols and Protic Arenosols: A Laboratory Experiment","volume":"55","author":"Sharapova","year":"2022","journal-title":"Eurasian Soil Sci. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1134\/S1061934819130045","article-title":"Use of Chemometric Methods of Data Analysis for the Identification and Typification of Petroleum and Petroleum Products","volume":"74","author":"Bolotnik","year":"2019","journal-title":"J. Anal. Chem."},{"key":"ref_22","unstructured":"Andrews, S. (2021, December 16). A Quality Control Tool for High Throughput Sequence Data. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"McMurdie, P.J., and Holmes, S. (2013). Phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061217"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/s40168-018-0605-2","article-title":"Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data","volume":"6","author":"Davis","year":"2018","journal-title":"Microbiome"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s40168-018-0521-5","article-title":"IDTAXA: A novel approach for accurate taxonomic classification of microbiome sequences","volume":"6","author":"Murali","year":"2018","journal-title":"Microbiome"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"352","DOI":"10.32614\/RJ-2016-025","article-title":"Using DECIPHER v2.0 to analyze big biological sequence data in R","volume":"8","author":"Wright","year":"2016","journal-title":"R J."},{"key":"ref_28","first-page":"D590","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Kim","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wright, E.S. (2015). DECIPHER: Harnessing local sequence context to improve protein multiple sequence alignment. BMC Bioinform., 16.","DOI":"10.1186\/s12859-015-0749-z"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2\u2014Approximately maximum-likelihood trees for large alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","article-title":"PICRUSt2 for prediction of metagenome functions","volume":"38","author":"Douglas","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/j.1654-1103.2003.tb02228.x","article-title":"VEGAN, a package of R functions for community ecology","volume":"14","author":"Dixon","year":"2003","journal-title":"J. Veg. Sci."},{"key":"ref_33","first-page":"1","article-title":"ALDEx2: ANOVA-Like Differential Expression tool for compositional data","volume":"20","author":"Gloor","year":"2019","journal-title":"ALDEX Man. Modul."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e01202-20","DOI":"10.1128\/mSphere.01202-20","article-title":"Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing","volume":"6","author":"Matchado","year":"2021","journal-title":"Msphere"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21390","DOI":"10.1073\/pnas.1215210110","article-title":"Cross-biome metagenomic analyses of soil microbial communities and their functional attributes","volume":"109","author":"Fierer","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1128\/AEM.72.3.1719-1728.2006","article-title":"Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes","volume":"72","author":"Janssen","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1134\/S1064229312020044","article-title":"Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques","volume":"45","author":"Andronov","year":"2012","journal-title":"Eurasian Soil Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"fix116","DOI":"10.1093\/femsec\/fix116","article-title":"Climate controls prokaryotic community composition in desert soils of the southwestern United States","volume":"93","author":"McHugh","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1128\/AEM.70.1.468-474.2004","article-title":"Bacterial Diversity in Agricultural Soils during Litter Decomposition","volume":"70","author":"Dilly","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1134\/S1064229318030092","article-title":"Changes in the Metagenome of Prokaryotic Community as an Indicator of Fertility of Arable Soddy-Podzolic Soils upon Fertilizer Application","volume":"51","author":"Naliukhin","year":"2018","journal-title":"Eurasian Soil Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1134\/S106422931908012X","article-title":"Biomass and Taxonomic Structure of Microbial Communities in Soils of the Right-Bank Basin of the Oka River","volume":"52","author":"Semenov","year":"2019","journal-title":"Eurasian Soil Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Vilkiene, M., Mockeviciene, I., Karcauskiene, D., Suproniene, S., Doyeni, M.O., and Ambrazaitiene, D. (2021). Biological indicators of soil quality under different tillage systems in retisol. Sustainability, 13.","DOI":"10.3390\/su13179624"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3389\/fmicb.2013.00215","article-title":"Controls on bacterial and archaeal community structure and greenhouse gas production in natural, mined, and restored Canadian peatlands","volume":"4","author":"Basiliko","year":"2013","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.apsoil.2013.09.010","article-title":"Bacterial diversity and community structure along different peat soils in boreal forest","volume":"74","author":"Sun","year":"2014","journal-title":"Appl. Soil Ecol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1134\/S0026261718010083","article-title":"Structure of microbial communities of peat soils in two bogs in Siberian tundra and forest zones","volume":"87","author":"Grodnitskaya","year":"2018","journal-title":"Microbiology"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Aksenov, A.S., Shirokova, L.S., Kisil, O.Y., Kolesova, S.N., Lim, A.G., Kuzmina, D., Pouill\u00e9, S., Alexis, M.A., Castrec-Rouelle, M., and Loiko, S.V. (2021). Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile. Diversity, 13.","DOI":"10.3390\/d13070328"},{"key":"ref_47","first-page":"2000440","article-title":"Insight into Washing of Wet and Dry Crude Oil-Contaminated Soil, Clean\u2014Soil, Air","volume":"49","author":"Qi","year":"2021","journal-title":"Water"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"127037","DOI":"10.1016\/j.colsurfa.2021.127037","article-title":"Liquid imbibition in tight rocks: The role of disjoining pressure, Colloids Surfaces A","volume":"627","author":"Yuan","year":"2021","journal-title":"Physicochem. Eng. Asp."},{"key":"ref_49","first-page":"39","article-title":"Effects of Refined Petroleum Hydrocarbon on Soil Physicochemical and Bacteriological Characteristics","volume":"6","author":"Obire","year":"2002","journal-title":"J. Appl. Sci. Environ. Manag."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1007\/s12665-015-4458-8","article-title":"Evaluation of petroleum impacts on some properties of loamy sand soil with the main focus on hydraulic properties","volume":"74","author":"Fallah","year":"2015","journal-title":"Environ. Earth Sci."},{"key":"ref_51","first-page":"2094","article-title":"Chronic Kerosene Contamination and Variation in the Physicochemical and Heavy Metal Content of the Soil in Calabar, Cross River State, Nigeria","volume":"2","author":"Nseabasi","year":"2016","journal-title":"Imp. J. Interdiscip. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AEM.02312-20","article-title":"No Endospore Formation Confirmed in Members of the Phylum Proteobacteria","volume":"87","author":"Beskrovnaya","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"37473","DOI":"10.1038\/srep37473","article-title":"Hydrocarbon degraders establish at the costs of microbial richness, abundance and keystone taxa after crude oil contamination in permafrost environments","volume":"6","author":"Yang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Shapiro, T., Chekanov, K., Alexandrova, A., Dolnikova, G., Ivanova, E., and Lobakova, E. (2021). Revealing of non-cultivable bacteria associated with the mycelium of fungi in the kerosene-degrading community isolated from the contaminated jet fuel. J. Fungi, 7.","DOI":"10.3390\/jof7010043"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/j.ibiod.2010.03.008","article-title":"Preferential degradation of aromatic hydrocarbons in kerosene by a microbial consortium","volume":"64","author":"Bacosa","year":"2010","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"10856","DOI":"10.1038\/s41598-021-89637-y","article-title":"Impact of diesel and biodiesel contamination on soil microbial community activity and structure","volume":"11","author":"Mitter","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"34987","DOI":"10.1007\/s11356-020-09754-5","article-title":"Polycyclic aromatic hydrocarbons (PAHs) and putative PAH-degrading bacteria in Galveston Bay, TX (USA), following Hurricane Harvey (2017)","volume":"27","author":"Bacosa","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.chemosphere.2019.05.290","article-title":"Enhanced degradation of polycyclic aromatic hydrocarbons (PAHs) in the rhizosphere of sudangrass (Sorghum \u00d7 drummondii)","volume":"234","author":"Dominguez","year":"2019","journal-title":"Chemosphere"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1080\/00207233.2020.1817276","article-title":"Microbial communities in field-scale oil-polluted soil remediation using 16S rRNA amplicon sequencing","volume":"78","author":"Chikere","year":"2021","journal-title":"Int. J. Environ. Stud."},{"key":"ref_60","first-page":"1","article-title":"Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica","volume":"30","author":"Shukor","year":"2009","journal-title":"J. Environ. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1462-2920.2005.00911.x","article-title":"Pseudomonas, the dominant polycyclic aromatic hydrocarbon-degrading bacteria isolated from Antarctic soils and the role of large plasmids in horizontal gene transfer","volume":"8","author":"Ma","year":"2006","journal-title":"Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Yang, S., Wen, X., Zhao, L., Shi, Y., and Jin, H. (2014). Crude oil treatment leads to shift of bacterial communities in soils from the deep active layer and upper permafrost along the China-Russia Crude Oil Pipeline route. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0096552"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1016\/j.jglr.2021.08.003","article-title":"Temporal variation of crude and refined oil biodegradation rates and microbial community composition in freshwater systems","volume":"47","author":"Byrne","year":"2021","journal-title":"J. Great Lakes Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2696","DOI":"10.3389\/fmicb.2018.02696","article-title":"Transcriptome-Stable Isotope Probing Provides Targeted Functional and taxonomic insights into microaerobic pollutant-degrading aquifer microbiota","volume":"9","author":"Bradford","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.resmic.2016.07.004","article-title":"Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation","volume":"167","author":"Wang","year":"2016","journal-title":"Res. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1038\/ismej.2014.151","article-title":"Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site","volume":"8","author":"Mukherjee","year":"2014","journal-title":"ISME J."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3389\/fmicb.2018.00520","article-title":"A retrospective review of microbiological methods applied in studies following the Deepwater Horizon oil spill","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_68","first-page":"1698","article-title":"Bacterial species associated with soils contaminated with used petroleum products in Keffi town, Nigeria","volume":"4","author":"Makut","year":"2010","journal-title":"Afr. J. Microbiol. Res."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/2\/221\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:11:54Z","timestamp":1760134314000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/2\/221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,31]]},"references-count":68,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["life12020221"],"URL":"https:\/\/doi.org\/10.3390\/life12020221","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,31]]}}}