{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T15:02:01Z","timestamp":1778770921537,"version":"3.51.4"},"reference-count":95,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,3,26]],"date-time":"2022-03-26T00:00:00Z","timestamp":1648252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA016058"],"award-info":[{"award-number":["CA016058"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"United States Department of Defense","doi-asserted-by":"publisher","award":["CA210874"],"award-info":[{"award-number":["CA210874"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Soft tissue sarcomas (STS) are rare malignancies with limited responses to anticancer therapy. Extracellular vesicles (EVs) are a heterogeneous group of bi-lipid layer sacs secreted by cells into extracellular space. Investigations of tumor-derived EVs have revealed their functional capabilities, including cell-to-cell communication and their impact on tumorigenesis, progression, and metastasis; however information on the roles of EVs in sarcoma is currently limited. In this review we investigate the role of various EV cargos in sarcoma and the mechanisms by which those cargos can affect the recipient cell phenotype and the aggressivity of the tumor itself. The study of EVs in sarcoma may help establish novel therapeutic approaches that target specific sarcoma subtypes or biologies, thereby improving sarcoma therapeutics in the future.<\/jats:p>","DOI":"10.3390\/life12040481","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:29:36Z","timestamp":1648416576000},"page":"481","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cancer-Derived Extracellular Vesicles: Their Role in Sarcoma"],"prefix":"10.3390","volume":"12","author":[{"given":"Anita","family":"Adib","sequence":"first","affiliation":[{"name":"The James Cancer Hospital Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruhi","family":"Sahu","sequence":"additional","affiliation":[{"name":"The James Cancer Hospital Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shivangi","family":"Mohta","sequence":"additional","affiliation":[{"name":"The James Cancer Hospital Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0443-1583","authenticated-orcid":false,"given":"Raphael Etomar","family":"Pollock","sequence":"additional","affiliation":[{"name":"Division of Surgical Oncology, Department of Surgery, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Casadei","sequence":"additional","affiliation":[{"name":"The James Cancer Hospital Solove Research Institute, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1093\/biosci\/biv084","article-title":"Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study","volume":"65","author":"Zaborowski","year":"2015","journal-title":"Bioscience"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s10555-016-9630-4","article-title":"The roles and implications of exosomes in sarcoma","volume":"35","author":"Min","year":"2016","journal-title":"Cancer Metastasis Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4146","DOI":"10.1073\/pnas.1200448109","article-title":"Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein","volume":"109","author":"Nabhan","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2018.02.013","article-title":"Biogenesis and function of extracellular vesicles in cancer","volume":"188","author":"Bebelman","year":"2018","journal-title":"Pharmacol. Ther."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1126\/science.1153124","article-title":"Ceramide triggers budding of exosome vesicles into multivesicular endosomes","volume":"319","author":"Trajkovic","year":"2008","journal-title":"Science"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.devcel.2011.05.015","article-title":"The ESCRT pathway","volume":"21","author":"Henne","year":"2011","journal-title":"Dev. Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1172\/JCI81135","article-title":"The biology and function of exosomes in cancer","volume":"126","author":"Kalluri","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ceb.2014.05.004","article-title":"Biogenesis and secretion of exosomes","volume":"29","author":"Kowal","year":"2014","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.devcel.2011.08.019","article-title":"The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis","volume":"21","author":"Charrin","year":"2011","journal-title":"Dev. Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/nrm2728","article-title":"Rab GTPases as coordinators of vesicle traffic","volume":"10","author":"Stenmark","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Doyle, L.M., and Wang, M.Z. (2019). Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis. Cells, 8.","DOI":"10.3390\/cells8070727"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.devcel.2007.04.005","article-title":"Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle","volume":"12","author":"Cai","year":"2007","journal-title":"Dev. Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/emboj.2009.45","article-title":"Acid sphingomyelinase activity triggers microparticle release from glial cells","volume":"28","author":"Bianco","year":"2009","journal-title":"EMBO J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1535750","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Witwer","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3389\/fimmu.2014.00442","article-title":"Tetraspanins in extracellular vesicle formation and function","volume":"5","author":"Andreu","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1074\/mcp.M000063-MCP201","article-title":"Proteomics analysis of bladder cancer exosomes","volume":"9","author":"Welton","year":"2010","journal-title":"Mol. Cell. Proteom."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ciardiello, C., Cavallini, L., Spinelli, C., Yang, J., Reis-Sobreiro, M., de Candia, P., Minciacchi, V.R., and Di Vizio, D. (2016). Focus on Extracellular Vesicles: New Frontiers of Cell-to-Cell Communication in Cancer. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17020175"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"20121","DOI":"10.1074\/jbc.273.32.20121","article-title":"Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes","volume":"273","author":"Escola","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1016\/j.yexcr.2008.09.020","article-title":"Trafficking and function of the tetraspanin CD63","volume":"315","author":"Pols","year":"2009","journal-title":"Exp. Cell Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.cub.2009.09.059","article-title":"ARF6-regulated shedding of tumor cell-derived plasma membrane microvesicles","volume":"19","author":"Clancy","year":"2009","journal-title":"Curr. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.7150\/thno.41580","article-title":"Progress, opportunity, and perspective on exosome isolation\u2014efforts for efficient exosome-based theranostics","volume":"10","author":"Yang","year":"2020","journal-title":"Theranostics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.13070\/mm.en.5.1450","article-title":"Exosomes: Isolation and Characterization Methods and Specific Markers","volume":"5","author":"Yakimchuk","year":"2015","journal-title":"Mater Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e12062","DOI":"10.1002\/jev2.12062","article-title":"Cross-flow microfiltration for isolation, selective capture and release of liposarcoma extracellular vesicles","volume":"10","author":"Casadei","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3790","DOI":"10.1039\/C8LC01071D","article-title":"Ultrasensitive quantification of tumor mRNAs in extracellular vesicles with an integrated microfluidic digital analysis chip","volume":"18","author":"Zhang","year":"2018","journal-title":"Lab Chip"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2003237","DOI":"10.1002\/adfm.202003237","article-title":"Coupling Nanostructured Microchips with Covalent Chemistry Enables Purification of Sarcoma-Derived Extracellular Vesicles for Downstream Functional Studies","volume":"30","author":"Dong","year":"2020","journal-title":"Adv. Funct. Mater"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"622048","DOI":"10.3389\/fcell.2020.622048","article-title":"Next-Generation Cancer Biomarkers: Extracellular Vesicle DNA as a Circulating Surrogate of Tumor DNA","volume":"8","author":"Amintas","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nature15756","article-title":"Tumour exosome integrins determine organotropic metastasis","volume":"527","author":"Hoshino","year":"2015","journal-title":"Nature"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1038\/nature14359","article-title":"Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth","volume":"520","author":"Raiborg","year":"2015","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1083\/jcb.201601025","article-title":"Cortactin promotes exosome secretion by controlling branched actin dynamics","volume":"214","author":"Sinha","year":"2016","journal-title":"J. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1083\/jcb.201703206","article-title":"Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling","volume":"217","author":"Verweij","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"14041","DOI":"10.1038\/ncomms14041","article-title":"Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23","volume":"8","author":"Wei","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.3389\/fonc.2019.01342","article-title":"The Emerging Roles of Extracellular Vesicles in Osteosarcoma","volume":"9","author":"Perut","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.bbcan.2017.11.005","article-title":"The key role of extracellular vesicles in the metastatic process","volume":"1869","author":"Zhao","year":"2018","journal-title":"Biochim. Biophys. Acta Rev. Cancer"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1182\/blood-2014-12-618025","article-title":"Exosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts","volume":"126","author":"Paggetti","year":"2015","journal-title":"Blood"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/onc.2013.560","article-title":"Differentiation of tumour-promoting stromal myofibroblasts by cancer exosomes","volume":"34","author":"Webber","year":"2015","journal-title":"Oncogene"},{"key":"ref_36","first-page":"722","article-title":"Ovarian cancer-derived extracellular vesicles affect normal human fibroblast behavior","volume":"19","author":"Giusti","year":"2018","journal-title":"Cancer Biol. Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.ccell.2014.09.005","article-title":"Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis","volume":"26","author":"Melo","year":"2014","journal-title":"Cancer Cell"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jabalee, J., Towle, R., and Garnis, C. (2018). The Role of Extracellular Vesicles in Cancer: Cargo, Function, and Therapeutic Implications. Cells, 7.","DOI":"10.3390\/cells7080093"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"16923","DOI":"10.18632\/oncotarget.7850","article-title":"Diagnostic and prognostic relevance of circulating exosomal miR-373, miR-200a, miR-200b and miR-200c in patients with epithelial ovarian cancer","volume":"7","author":"Meng","year":"2016","journal-title":"Oncotarget"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11060-017-2639-x","article-title":"A microRNA signature from serum exosomes of patients with glioma as complementary diagnostic biomarker","volume":"136","author":"Santangelo","year":"2018","journal-title":"J. Neurooncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"24668","DOI":"10.18632\/oncotarget.14969","article-title":"MiR-21-5p in urinary extracellular vesicles is a novel biomarker of urothelial carcinoma","volume":"8","author":"Matsuzaki","year":"2017","journal-title":"Oncotarget"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12169","DOI":"10.1007\/s13277-016-5071-5","article-title":"Exosomes derived from gastric cancer cells activate NF-\u03baB pathway in macrophages to promote cancer progression","volume":"37","author":"Wu","year":"2016","journal-title":"Tumor Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.canlet.2014.03.009","article-title":"Exosome-mediated crosstalk between chronic myelogenous leukemia cells and human bone marrow stromal cells triggers an interleukin 8-dependent survival of leukemia cells","volume":"348","author":"Corrado","year":"2014","journal-title":"Cancer Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13045-015-0181-x","article-title":"Exosomes in cancer: Small particle, big player","volume":"8","author":"Zhang","year":"2015","journal-title":"J. Hematol. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6735","DOI":"10.1158\/1078-0432.CCR-07-4843","article-title":"The interleukin-8 pathway in cancer","volume":"14","author":"Waugh","year":"2008","journal-title":"Clin. Cancer Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"50086","DOI":"10.18632\/oncotarget.10574","article-title":"Exosomes from human colorectal cancer induce a tumor-like behavior in colonic mesenchymal stromal cells","volume":"7","author":"Lugini","year":"2016","journal-title":"Oncotarget"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/labinvest.2009.94","article-title":"Paracrine induction of endothelium by tumor exosomes","volume":"89","author":"Hood","year":"2009","journal-title":"Lab. Investig."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3792","DOI":"10.1158\/0008-5472.CAN-10-4455","article-title":"Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis","volume":"71","author":"Hood","year":"2011","journal-title":"Cancer Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.ccell.2016.10.009","article-title":"Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis","volume":"30","author":"Becker","year":"2016","journal-title":"Cancer Cell"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1016\/j.cell.2012.11.024","article-title":"Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration","volume":"151","author":"Luga","year":"2012","journal-title":"Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/j.cell.2015.04.042","article-title":"In Vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior","volume":"161","author":"Zomer","year":"2015","journal-title":"Cell"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Nazimek, K., and Bryniarski, K. (2020). Perspectives in Manipulating EVs for Therapeutic Applications: Focus on Cancer Treatment. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21134623"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10555-017-9712-y","article-title":"MicroRNAs and metastasis: Small RNAs play big roles","volume":"37","author":"Kim","year":"2018","journal-title":"Cancer Metastasis Rev."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Gilligan, K.E., and Dwyer, R.M. (2017). Engineering Exosomes for Cancer Therapy. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061122"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"531","DOI":"10.2147\/IJN.S191313","article-title":"Drug-loaded exosomal preparations from different cell types exhibit distinctive loading capability, yield, and antitumor efficacies: A comparative analysis","volume":"14","author":"Kanchanapally","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"8001","DOI":"10.7150\/thno.37097","article-title":"Extracellular vesicle-based drug delivery systems for cancer treatment","volume":"9","author":"Walker","year":"2019","journal-title":"Theranostics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1016\/j.joca.2016.06.022","article-title":"Exosomes derived from human embryonic mesenchymal stem cells promote osteochondral regeneration","volume":"24","author":"Zhang","year":"2016","journal-title":"Osteoarthr. Cartil."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"eaav8521","DOI":"10.1126\/scitranslmed.aav8521","article-title":"Advances in therapeutic applications of extracellular vesicles","volume":"11","author":"Wiklander","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nbt.1807","article-title":"Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes","volume":"29","author":"Seow","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1053\/j.semdp.2014.01.002","article-title":"Ewing sarcoma","volume":"31","author":"Choi","year":"2014","journal-title":"Semin. Diagn. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Kunisada, T., Nakata, E., Fujiwara, T., Hosono, A., Takihira, S., Kondo, H., and Ozaki, T. (2022). Soft-tissue sarcoma in adolescents and young adults. Int. J. Clin. Oncol.","DOI":"10.1007\/s10147-022-02119-7"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Tsugita, M., Yamada, N., Noguchi, S., Yamada, K., Moritake, H., Shimizu, K., Akao, Y., and Ohno, T. (2013). Ewing sarcoma cells secrete EWS\/Fli-1 fusion mRNA via microvesicles. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077416"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/boc.201200086","article-title":"First identification of Ewing\u2019s sarcoma-derived extracellular vesicles and exploration of their biological and potential diagnostic implications","volume":"105","author":"Miller","year":"2013","journal-title":"Biol. Cell"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Chic\u00f3n-Bosch, M., and Tirado, O.M. (2020). Exosomes in Bone Sarcomas: Key Players in Metastasis. Cells, 9.","DOI":"10.3390\/cells9010241"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3944","DOI":"10.1038\/onc.2015.463","article-title":"CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-\u03baB signaling","volume":"35","author":"Ventura","year":"2016","journal-title":"Oncogene"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/s41419-019-1675-1","article-title":"Exosomes from CD99-deprived Ewing sarcoma cells reverse tumor malignancy by inhibiting cell migration and promoting neural differentiation","volume":"10","author":"Sciandra","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/pbc.24118","article-title":"Rhabdomyosarcoma: Review of the Children\u2019s Oncology Group (COG) Soft-Tissue Sarcoma Committee experience and rationale for current COG studies","volume":"59","author":"Malempati","year":"2012","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1002\/pbc.24488","article-title":"Survival following disease recurrence of primary localized alveolar rhabdomyosarcoma","volume":"60","author":"Dantonello","year":"2013","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"3567","DOI":"10.1021\/acs.jproteome.9b00157","article-title":"Proteomic Profiling of Rhabdomyosarcoma-Derived Exosomes Yield Insights into Their Functional Role in Paracrine Signaling","volume":"18","author":"Rammal","year":"2019","journal-title":"J. Proteome Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"37088","DOI":"10.1038\/srep37088","article-title":"Exosomes derived from embryonal and alveolar rhabdomyosarcoma carry differential miRNA cargo and promote invasion of recipient fibroblasts","volume":"6","author":"Ghayad","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"14242","DOI":"10.1038\/s41598-019-50592-4","article-title":"The PAX3-FOXO1 oncogene alters exosome miRNA content and leads to paracrine effects mediated by exosomal miR-486","volume":"9","author":"Ghamloush","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1038\/s41388-017-0081-3","article-title":"PAX3-FOXO1 drives miR-486-5p and represses miR-221 contributing to pathogenesis of alveolar rhabdomyosarcoma","volume":"37","author":"Hanna","year":"2018","journal-title":"Oncogene"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Chugh, P.E., Sin, S.H., Ozgur, S., Henry, D.H., Menezes, P., Griffith, J., Eron, J.J., Damania, B., and Dittmer, D.P. (2013). Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003484"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/nrc2888","article-title":"Kaposi\u2019s sarcoma and its associated herpesvirus","volume":"10","author":"Mesri","year":"2010","journal-title":"Nat. Rev. Cancer"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"99841","DOI":"10.18632\/oncotarget.21668","article-title":"Extracellular vesicles from KSHV-infected endothelial cells activate the complement system","volume":"8","author":"Jeon","year":"2017","journal-title":"Oncotarget"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"4417","DOI":"10.1128\/JVI.03282-12","article-title":"Kaposi\u2019s sarcoma-associated herpesvirus latency in endothelial and B cells activates gamma interferon-inducible protein 16-mediated inflammasomes","volume":"87","author":"Singh","year":"2013","journal-title":"J. Virol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"E2925","DOI":"10.1073\/pnas.1303906110","article-title":"Modulation of B-cell exosome proteins by gamma herpesvirus infection","volume":"110","author":"Meckes","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3721","DOI":"10.1158\/1078-0432.CCR-16-2726","article-title":"Blocking Tumor-Educated MSC Paracrine Activity Halts Osteosarcoma Progression","volume":"23","author":"Baglio","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Endo-Munoz, L., Cai, N., Cumming, A., Macklin, R., Merida de Long, L., Topkas, E., Mukhopadhyay, P., Hill, M., and Saunders, N.A. (2015). Progression of Osteosarcoma from a Non-Metastatic to a Metastatic Phenotype Is Causally Associated with Activation of an Autocrine and Paracrine uPA Axis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0133592"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Cappariello, A., and Rucci, N. (2019). Tumour-Derived Extracellular Vesicles (EVs): A Dangerous \u201cMessage in A Bottle\u201d for Bone. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194805"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.tranon.2014.04.011","article-title":"Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment","volume":"7","author":"Garimella","year":"2014","journal-title":"Transl. Oncol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.jss.2014.11.025","article-title":"Serum miR-195 is a diagnostic and prognostic marker for osteosarcoma","volume":"194","author":"Cai","year":"2015","journal-title":"J. Surg. Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"12467","DOI":"10.1007\/s13277-014-2565-x","article-title":"Circulating miR-148a is a significant diagnostic and prognostic biomarker for patients with osteosarcoma","volume":"35","author":"Ma","year":"2014","journal-title":"Tumour Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"33375","DOI":"10.18632\/oncotarget.16498","article-title":"Clinical significance of circulating miR-25-3p as a novel diagnostic and prognostic biomarker in osteosarcoma","volume":"8","author":"Fujiwara","year":"2017","journal-title":"Oncotarget"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.gene.2019.06.005","article-title":"Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis","volume":"710","author":"Jerez","year":"2019","journal-title":"Gene"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.bbrc.2018.04.016","article-title":"Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1","volume":"500","author":"Gong","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/carcin\/bgz130","article-title":"Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRNAs","volume":"41","author":"Raimondi","year":"2020","journal-title":"Carcinogenesis"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1080\/09674845.2016.1220710","article-title":"miR-21 predicts poor prognosis in patients with osteosarcoma","volume":"73","author":"Ren","year":"2016","journal-title":"Br. J. Biomed. Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.3727\/096504017X14841698396829","article-title":"MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8","volume":"25","author":"Xu","year":"2017","journal-title":"Oncol. Res."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Miettinen, M. (2016). Modern Soft Tissue Pathology: Chapter 15\u2014Atypical Lipomatous Tumors and Liposarcomas, Cambridge University Press.","DOI":"10.1017\/9781107707504"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.canlet.2020.04.026","article-title":"Extracellular vesicle cross-talk in the liposarcoma microenvironment","volume":"487","author":"Casadei","year":"2020","journal-title":"Cancer Lett."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"10562","DOI":"10.1200\/jco.2014.32.15_suppl.10562","article-title":"Detection of MDM2 gene amplification in soft tissue sarcoma by FISH","volume":"32","author":"Kimura","year":"2014","journal-title":"J. Clin. Oncol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nrc991","article-title":"Inhibiting the p53-MDM2 interaction: An important target for cancer therapy","volume":"3","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"4911","DOI":"10.1158\/0008-5472.CAN-19-0203","article-title":"Derived from Dedifferentiated Liposarcoma Extracellular Vesicles Induces MMP2 Production from Preadipocytes","volume":"79","author":"Casadei","year":"2019","journal-title":"Cancer Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1158\/0008-5472.CAN-16-2984","article-title":"Exosome-Derived miR-25-3p and miR-92a-3p Stimulate Liposarcoma Progression","volume":"77","author":"Casadei","year":"2017","journal-title":"Cancer Res."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/4\/481\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:44:00Z","timestamp":1760136240000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/4\/481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,26]]},"references-count":95,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["life12040481"],"URL":"https:\/\/doi.org\/10.3390\/life12040481","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,26]]}}}