{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:40:46Z","timestamp":1791520846369,"version":"4.3.4"},"reference-count":75,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Taif University","award":["TURSP-2020\/258"],"award-info":[{"award-number":["TURSP-2020\/258"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The objective of this study was to evaluate the Glycyrrhiza glabra effect on growth performance, blood parameters, antioxidant and lysosomal activity, histology and immunohistochemistry of liver and intestine, and the gene expression profile of broiler chickens. A total of 180 Cobb500 broiler chicks (one-week-old) were used in this study. Chicks were distributed randomly into three treatment groups; the first group received drinking water without any supplementation (control group). In contrast, birds in groups 2 and 3 received licorice supplementation in drinking water with 0.4 and 0.8 g licorice\/liter, respectively. Results revealed that licorice at a 0.4 g\/L of water level improved body weight, weight gain, feed intake, and FCR. Licorice also exhibits a broad range of biological activities such as hypolipidemic, hypoglycemic, hepatoprotective, immunostimulant, and antioxidant effects. The morphometric analysis of different parameters of the intestine revealed a significant increase in the intestinal villi length, width, and villi length\/crypt depth in the group supplemented with licorice 0.4 gm\/L compared to other groups. The number of CD3 positive in both duodenum and ileum was increased in the licorice 0.4 gm\/L group compared to other groups. The expression of growth-related genes was significantly increased with licorice supplementation and modulation of the lipid metabolism genes in the liver and upregulated to the mRNA expression of both superoxide dismutase (SOD1) and Catalase (CAT). Our results revealed that licorice supplementation increased the growth performance of broiler chickens and impacted the birds\u2019 antioxidant activity through modulation of the growth-related genes, lipid metabolic markers, and antioxidant-related pathways.<\/jats:p>","DOI":"10.3390\/life12060914","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T11:45:44Z","timestamp":1655466344000},"page":"914","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Licorice Extract Supplementation Affects Antioxidant Activity, Growth-Related Genes, Lipid Metabolism, and Immune Markers in Broiler Chickens"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8011-554X","authenticated-orcid":false,"given":"Magda I.","family":"Abo-Samaha","sequence":"first","affiliation":[{"name":"Poultry Breeding and Production, Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef S.","family":"Alghamdi","sequence":"additional","affiliation":[{"name":"Department of Biology, Turabah University College, Taif University, P.O. Box 11099, Taif 21995, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Set A.","family":"El-Shobokshy","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0774-5550","authenticated-orcid":false,"given":"Sarah","family":"Albogami","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman M. Abd","family":"El-Maksoud","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foad","family":"Farrag","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7208-7123","authenticated-orcid":false,"given":"Mohamed M.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Sciences Department, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2722-2466","authenticated-orcid":false,"given":"Mustafa","family":"Shukry","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2831-8534","authenticated-orcid":false,"given":"Mohamed E. Abd","family":"El-Hack","sequence":"additional","affiliation":[{"name":"Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.prevetmed.2009.09.012","article-title":"Changes in antimicrobial resistance among faecal enterococci isolated from growing broilers prophylactically medicated with three commercial antimicrobials","volume":"93","author":"Bica","year":"2010","journal-title":"Prev. Vet. Med."},{"key":"ref_2","first-page":"41","article-title":"Drinking water supplementation of licorice and aloe vera extracts in broiler chickens","volume":"3","author":"Salary","year":"2014","journal-title":"Sci. J. Anim. 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