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Planning\/the Ministry of Education, Science and Technology","award":["SMX1210881"],"award-info":[{"award-number":["SMX1210881"]}]},{"name":"SMC-Ottogi Research Fund","award":["NRF-2020R1F1A1072603"],"award-info":[{"award-number":["NRF-2020R1F1A1072603"]}]},{"name":"SMC-Ottogi Research Fund","award":["HI14C3484"],"award-info":[{"award-number":["HI14C3484"]}]},{"name":"SMC-Ottogi Research Fund","award":["SMX122081"],"award-info":[{"award-number":["SMX122081"]}]},{"name":"SMC-Ottogi Research Fund","award":["2020R1A2C2010645"],"award-info":[{"award-number":["2020R1A2C2010645"]}]},{"name":"SMC-Ottogi Research Fund","award":["SMX1210881"],"award-info":[{"award-number":["SMX1210881"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>We recently reported that transplantation of mesenchymal stem cells (MSCs) significantly reduced bacterial growth and brain injury in neonatal meningitis induced by Escherichia coli (E. coli) infection in newborn rats. As a next step, to verify whether the MSCs protect against brain injury in a paracrine manner, this study was designed to estimate the efficacy of MSC-derived extracellular vesicles (MSC-EVs) in E. coli meningitis in newborn rats. E. coli meningitis was induced without concomitant bacteremia by the intra-cerebroventricular injection of 5 \u00d7 102 colony-forming units of K1 (-) E. coli in rats, at postnatal day 11. MSC-EVs were intra-cerebroventricularly transplanted 6 h after the induction of meningitis, and antibiotics were administered for three consecutive days starting at 24 h after the induction of meningitis. The increase in bacterial growth in the cerebrospinal fluid measured at 24 h after the meningitis induction was not significantly reduced following MSC-EV transplantation. However, an increase in brain cell death, reactive gliosis, and inflammation following meningitis were significantly attenuated after MSC-EV transplantation. Taken together, our results indicate that MSCs show anti-apoptotic, anti-gliosis, and anti-inflammatory, but not antibacterial effects, in an EV-mediated paracrine manner in E. coli-induced neonatal meningitis.<\/jats:p>","DOI":"10.3390\/life12071030","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T21:57:53Z","timestamp":1657576673000},"page":"1030","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Attenuate Brain Injury in Escherichia coli Meningitis in Newborn Rats"],"prefix":"10.3390","volume":"12","author":[{"given":"Young-Eun","family":"Kim","sequence":"first","affiliation":[{"name":"Cell and Gene Therapy Institute, Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology (SAIHST), Samsung Medical Center, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Yoon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Soon","family":"Park","sequence":"additional","affiliation":[{"name":"Cell and Gene Therapy Institute, Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology (SAIHST), Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Kyung","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se-In","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Sun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9201-2938","authenticated-orcid":false,"given":"Yun-Sil","family":"Chang","sequence":"additional","affiliation":[{"name":"Cell and Gene Therapy Institute, Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology (SAIHST), Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1097\/INF.0000000000002247","article-title":"Canadian Neonatal Network, I. 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Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/s41390-018-0142-5","article-title":"Mesenchymal stem cells transplantation attenuates brain injury and enhances bacterial clearance in Escherichia coli meningitis in newborn rats","volume":"84","author":"Ahn","year":"2018","journal-title":"Pediatr. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s12026-016-8798-6","article-title":"Immunomodulatory effects of mesenchymal stromal cells-derived exosome","volume":"64","author":"Chen","year":"2016","journal-title":"Immunol. Res."},{"key":"ref_10","first-page":"1","article-title":"Vascular endothelial growth factor mediates the therapeutic efficacy of mesenchymal stem cell-derived extracellular vesicles against neonatal hyperoxic lung injury","volume":"50","author":"Ahn","year":"2018","journal-title":"Exp. Mol. 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