{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:43:17Z","timestamp":1791585797693,"version":"4.3.4"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Laboratory of Developmental Biology of Freshwater Fish","award":["81801392"],"award-info":[{"award-number":["81801392"]}]},{"name":"State Key Laboratory of Developmental Biology of Freshwater Fish","award":["32100919"],"award-info":[{"award-number":["32100919"]}]},{"name":"State Key Laboratory of Developmental Biology of Freshwater Fish","award":["32071175"],"award-info":[{"award-number":["32071175"]}]},{"name":"National Natural Science Foundation of China","award":["81801392"],"award-info":[{"award-number":["81801392"]}]},{"name":"National Natural Science Foundation of China","award":["32100919"],"award-info":[{"award-number":["32100919"]}]},{"name":"National Natural Science Foundation of China","award":["32071175"],"award-info":[{"award-number":["32071175"]}]},{"name":"Key Project of Developmental Biology and Breeding from Hunan Province","award":["81801392"],"award-info":[{"award-number":["81801392"]}]},{"name":"Key Project of Developmental Biology and Breeding from Hunan Province","award":["32100919"],"award-info":[{"award-number":["32100919"]}]},{"name":"Key Project of Developmental Biology and Breeding from Hunan Province","award":["32071175"],"award-info":[{"award-number":["32071175"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Excessive alcohol consumption can cause alcoholic myopathy, but the molecular mechanism is still unclear. In this study, zebrafish were exposed to 0.5% alcohol for eight weeks to investigate the effect of alcohol on skeletal muscle and its molecular mechanism. The results showed that the body length, body weight, cross-sectional area of the skeletal muscle fibers, Ucrit, and MO2max of the zebrafish were significantly decreased after alcohol exposure. The expression of markers of skeletal muscle atrophy and autophagy was increased, and the expression of P62 was significantly reduced. The content of ROS, the mRNA expression of sod1 and sod2, and the protein expression of Nox2 were significantly increased. In addition, we found that the inflammatory factors Il1\u03b2 and Tnf\u03b1 were significantly enriched in skeletal muscle, and the expression of the HMGB1\/TLR4\/NF-\u03baB signaling axis was also significantly increased. In summary, in this study, we established a zebrafish model of alcohol-induced skeletal muscle atrophy and further elucidated its pathogenesis.<\/jats:p>","DOI":"10.3390\/life12081211","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T04:20:32Z","timestamp":1660105232000},"page":"1211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Alcohol Induces Zebrafish Skeletal Muscle Atrophy through HMGB1\/TLR4\/NF-\u03baB Signaling"],"prefix":"10.3390","volume":"12","author":[{"given":"Wei","family":"Wen","sequence":"first","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenchen","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0724-6989","authenticated-orcid":false,"given":"Zhanglin","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuoqiong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1307-9785","authenticated-orcid":false,"given":"Xiyang","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changfa","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, Changsha 410012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1111\/j.1360-0443.2010.02899.x","article-title":"The relation between different dimensions of alcohol consumption and burden of disease: An overview","volume":"105","author":"Rehm","year":"2010","journal-title":"Addiction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/mus.20883","article-title":"Oxidant-induced atrogin-1 and transforming growth factor-beta1 precede alcohol-related myopathy in rats","volume":"36","author":"Otis","year":"2007","journal-title":"Muscle Nerve"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/S1357-2725(00)00081-9","article-title":"Alcohol myopathy: Impairment of protein synthesis and translation initiation","volume":"33","author":"Lang","year":"2001","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Levitt, D.E., Yeh, A.Y., Prendergast, M.J., Budnar, R.G., Adler, K.A., Cook, G., Molina, P.E., and Simon, L. (2020). Chronic Alcohol Dysregulates Skeletal Muscle Myogenic Gene Expression after Hind Limb Immobilization in Female Rats. Biomolecules, 10.","DOI":"10.3390\/biom10030441"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1111\/j.1530-0277.2007.00530.x","article-title":"Molecular and cellular events in alcohol-induced muscle disease","volume":"31","author":"Preedy","year":"2007","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"677","DOI":"10.4161\/auto.27918","article-title":"Alcohol-induced autophagy contributes to loss in skeletal muscle mass","volume":"10","author":"Thapaliya","year":"2014","journal-title":"Autophagy"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"E916","DOI":"10.1152\/ajpendo.00554.2003","article-title":"IGF-I\/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, and myostatin in alcohol-fed rats","volume":"286","author":"Lang","year":"2004","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"E1497","DOI":"10.1152\/ajpendo.00603.2006","article-title":"Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication","volume":"292","author":"Lang","year":"2007","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1111\/j.1530-0277.2009.00975.x","article-title":"Procysteine stimulates expression of key anabolic factors and reduces plantaris atrophy in alcohol-fed rats","volume":"33","author":"Otis","year":"2009","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.1530-0277.2007.00549.x","article-title":"Chronic alcohol accentuates simian acquired immunodeficiency syndrome-associated wasting","volume":"32","author":"Molina","year":"2008","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"C1291","DOI":"10.1152\/ajpcell.00531.2009","article-title":"Autophagy in health and disease. 3. Involvement of autophagy in muscle atrophy","volume":"298","author":"Sandri","year":"2010","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Lee, J.H., Jeon, J.H., and Lee, M.J. (2020). Docosahexaenoic Acid, a Potential Treatment for Sarcopenia, Modulates the Ubiquitin-Proteasome and the Autophagy-Lysosome Systems. Nutrients, 12.","DOI":"10.3390\/nu12092597"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"91","DOI":"10.33594\/000000327","article-title":"Multiomics-Identified Intervention to Restore Ethanol-Induced Dysregulated Proteostasis and Secondary Sarcopenia in Alcoholic Liver Disease","volume":"55","author":"Singh","year":"2021","journal-title":"Cell Physiol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/s00394-019-01933-6","article-title":"Creatine supplementation in Walker-256 tumor-bearing rats prevents skeletal muscle atrophy by attenuating systemic inflammation and protein degradation signaling","volume":"59","author":"Cella","year":"2020","journal-title":"Eur. J. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.lfs.2016.11.033","article-title":"TNF-\u03b1 and cancer cachexia: Molecular insights and clinical implications","volume":"170","author":"Patel","year":"2017","journal-title":"Life Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"597675","DOI":"10.3389\/fphys.2020.597675","article-title":"Inflammation and Skeletal Muscle Wasting During Cachexia","volume":"11","author":"Webster","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2998","DOI":"10.1111\/bph.15472","article-title":"Triptolide prevents LPS-induced skeletal muscle atrophy via inhibiting NF-\u03baB\/TNF-\u03b1 and regulating protein synthesis\/degradation pathway","volume":"178","author":"Fang","year":"2021","journal-title":"Br. J. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1152\/japplphysiol.91505.2008","article-title":"A novel hindlimb immobilization procedure for studying skeletal muscle atrophy and recovery in mouse","volume":"106","author":"Caron","year":"2009","journal-title":"J. Appl. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4465","DOI":"10.3390\/nu7064465","article-title":"NF-\u03baBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients","volume":"7","author":"Camargo","year":"2015","journal-title":"Nutrients"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1007\/s00109-008-0373-8","article-title":"Nuclear factor-kappa B signaling in skeletal muscle atrophy","volume":"86","author":"Li","year":"2008","journal-title":"J. Mol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Coffey, E.C., Pasquarella, M.E., Goody, M.F., and Henry, C.A. (2018). Ethanol Exposure Causes Muscle Degeneration in Zebrafish. J. Dev. Biol., 6.","DOI":"10.3390\/jdb6010007"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2014.05.001","article-title":"HMGB1 in health and disease","volume":"40","author":"Kang","year":"2014","journal-title":"Mol. Aspects Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1007\/s12272-012-0901-5","article-title":"Role of high mobility group box 1 in inflammatory disease: Focus on sepsis","volume":"35","author":"Bae","year":"2012","journal-title":"Arch. Pharm. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"484","DOI":"10.3389\/fimmu.2020.00484","article-title":"Targeting Inflammation Driven by HMGB1","volume":"11","author":"Yang","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104792","DOI":"10.1016\/j.phrs.2020.104792","article-title":"Implication of HMGB1 signaling pathways in Amyotrophic lateral sclerosis (ALS): From molecular mechanisms to pre-clinical results","volume":"156","author":"Paudel","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.alcohol.2021.05.003","article-title":"Serum HMGB1 associates with liver disease and predicts readmission and mortality in patients with alcohol use disorder","volume":"95","author":"Vannier","year":"2021","journal-title":"Alcohol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1002\/hep.30617","article-title":"Biomarkers of Macrophage Activation and Immune Danger Signals Predict Clinical Outcomes in Alcoholic Hepatitis","volume":"70","author":"Saha","year":"2019","journal-title":"Hepatology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0091-3057(02)01026-2","article-title":"Ethanol effects on three strains of zebrafish: Model system for genetic investigations","volume":"74","author":"Dlugos","year":"2003","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ejphar.2006.08.005","article-title":"Effects of chronic ethanol administration on brain protein levels: A proteomic investigation using 2-D DIGE system","volume":"547","author":"Damodaran","year":"2006","journal-title":"Eur. J. Pharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Morikane, D., Zang, L., and Nishimura, N. (2020). Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish. Molecules, 25.","DOI":"10.3390\/molecules25173974"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.bbrc.2016.09.117","article-title":"Chronic alcohol exposure induces muscle atrophy (myopathy) in zebrafish and alters the expression of microRNAs targeting the Notch pathway in skeletal muscle","volume":"479","author":"Khayrullin","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12576-021-00818-w","article-title":"Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart","volume":"71","author":"Chen","year":"2021","journal-title":"J. Physiol. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1242\/dmm.010389","article-title":"Cellular and molecular mechanisms of muscle atrophy","volume":"6","author":"Bonaldo","year":"2013","journal-title":"Dis. Model Mech."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0376-8716(00)00219-2","article-title":"Alcoholic myopathy: Biochemical mechanisms","volume":"63","author":"Preedy","year":"2001","journal-title":"Drug Alcohol Depend."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1007\/s00198-009-0836-y","article-title":"Alcohol alters whole body composition, inhibits bone formation, and increases bone marrow adiposity in rats","volume":"20","author":"Maddalozzo","year":"2009","journal-title":"Osteoporos. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1111\/acer.13476","article-title":"Increased Autolysis of \u03bc-Calpain in Skeletal Muscles of Chronic Alcohol-Fed Rats","volume":"41","author":"Gritsyna","year":"2017","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.ntt.2010.03.001","article-title":"Zebrafish embryos exposed to alcohol undergo abnormal development of motor neurons and muscle fibers","volume":"32","author":"Sylvain","year":"2010","journal-title":"Neurotoxicol. Teratol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Laudato, J.A., Tice, A.L., Call, J.A., Gordon, B.S., and Steiner, J.L. (2021). Effects of alcohol on skeletal muscle contractile performance in male and female mice. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0255946"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1002\/hep.31524","article-title":"Activated Protein Phosphatase 2A Disrupts Nutrient Sensing Balance Between Mechanistic Target of Rapamycin Complex 1 and Adenosine Monophosphate-Activated Protein Kinase, Causing Sarcopenia in Alcohol-Associated Liver Disease","volume":"73","author":"Davuluri","year":"2021","journal-title":"Hepatology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4294","DOI":"10.1111\/febs.12253","article-title":"Mechanisms regulating skeletal muscle growth and atrophy","volume":"280","author":"Schiaffino","year":"2013","journal-title":"FEBS J."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Chen, Y.H., Chiu, W.C., Xiao, Q., Chen, Y.L., Shirakawa, H., and Yang, S.C. (2021). Synbiotics Alleviate Hepatic Damage, Intestinal Injury and Muscular Beclin-1 Elevation in Rats after Chronic Ethanol Administration. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212547"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/acer.13531","article-title":"Effect of Chronic Alcohol Abuse on Anabolic and Catabolic Signaling Pathways in Human Skeletal Muscle","volume":"42","author":"Shenkman","year":"2018","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/acer.14221","article-title":"Chronic Alcohol Consumption Enhances Skeletal Muscle Wasting in Mice Bearing Cachectic Cancers: The Role of TNF\u03b1\/Myostatin Axis","volume":"44","author":"Li","year":"2020","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.clnu.2008.04.007","article-title":"Muscle atrophy, inflammation and clinical outcome in incident and prevalent dialysis patients","volume":"27","author":"Carrero","year":"2008","journal-title":"Clin. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"C135","DOI":"10.1152\/ajpcell.00111.2012","article-title":"Rel A\/p65 is required for cytokine-induced myotube atrophy","volume":"303","author":"Yamaki","year":"2012","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_46","first-page":"2567150","article-title":"Curcumin Targeting NF-\u03baB\/Ubiquitin-Proteasome-System Axis Ameliorates Muscle Atrophy in Triple-Negative Breast Cancer Cachexia Mice","volume":"2022","author":"Zhang","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1111\/acer.12771","article-title":"Alcohol Differentially Alters Extracellular Matrix and Adhesion Molecule Expression in Skeletal Muscle and Heart","volume":"39","author":"Steiner","year":"2015","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3095","DOI":"10.1002\/j.1460-2075.1993.tb05978.x","article-title":"Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene-inductive effects of TNF","volume":"12","author":"Beyaert","year":"1993","journal-title":"EMBO J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1038\/sj.onc.1207867","article-title":"Interleukin-1beta stimulates IL-8 expression through MAP kinase and ROS signaling in human gastric carcinoma cells","volume":"23","author":"Hwang","year":"2004","journal-title":"Oncogene"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"107272","DOI":"10.1016\/j.intimp.2020.107272","article-title":"Effect of gut microbiota on LPS-induced acute lung injury by regulating the TLR4\/NF-kB signaling pathway","volume":"91","author":"Tang","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_51","first-page":"1237","article-title":"Exendin-4 inhibits lipotoxicity-induced oxidative stress in \u03b2-cells by inhibiting the activation of TLR4\/NF-\u03baB signaling pathway","volume":"45","author":"Shen","year":"2020","journal-title":"Int. J. Mol. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5657","DOI":"10.1074\/jbc.M506172200","article-title":"NF-kappaB regulates phagocytic NADPH oxidase by inducing the expression of gp91phox","volume":"281","author":"Anrather","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.freeradbiomed.2019.09.031","article-title":"Oxidative stress mediates ethanol-induced skeletal muscle mitochondrial dysfunction and dysregulated protein synthesis and autophagy","volume":"145","author":"Kumar","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.neuropharm.2017.01.031","article-title":"The role of neuroimmune signaling in alcoholism","volume":"122","author":"Crews","year":"2017","journal-title":"Neuropharmacology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.biopsych.2012.09.030","article-title":"High mobility group box 1\/Toll-like receptor danger signaling increases brain neuroimmune activation in alcohol dependence","volume":"73","author":"Crews","year":"2013","journal-title":"Biol. Psychiatry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1096\/fj.10-164152","article-title":"Toll-like receptor 4 mediates lipopolysaccharide-induced muscle catabolism via coordinate activation of ubiquitin-proteasome and autophagy-lysosome pathways","volume":"25","author":"Doyle","year":"2011","journal-title":"FASEB J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1172\/JCI200421696","article-title":"Disruption of either the Nfkb1 or the Bcl3 gene inhibits skeletal muscle atrophy","volume":"114","author":"Hunter","year":"2004","journal-title":"J. Clin. Investig."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/8\/1211\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:06:31Z","timestamp":1760141191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/12\/8\/1211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,10]]},"references-count":57,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["life12081211"],"URL":"https:\/\/doi.org\/10.3390\/life12081211","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,10]]}}}