{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:22:35Z","timestamp":1790050955290,"version":"4.0.1"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China\u2019s 13th Five-Year key research and development project","award":["2016YFD0501000"],"award-info":[{"award-number":["2016YFD0501000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Gene-edited dogs are promising models for biomedical research because they have hundreds of genetic diseases that are similar to humans. A common method for producing gene-edited dogs is assisted reproductive technology (ART) using in vivo oocytes or embryos, but it is much more inefficient and has a higher cost. ART for dogs has lagged mostly because of the lack of an efficient in vitro maturation system. Because early maturation of canine oocytes occurs in follicles with extremely high concentrations of progesterone (P4), we hypothesize that P4 has an important role during maturation. In this study, we obtained ovaries of female dogs and collected cumulus\u2013oocyte complexes, which were cultured in vitro in microdrops containing different P4 concentrations (0, 10, 40, 100 or 200 \u00b5g\/mL). We found that 40 \u00b5g\/mL P4 produced the highest oocyte maturation rate (29.7% \u00b1 7.1%, p &lt; 0.05). We also evaluated the quality of in vitro matured oocytes by in vitro fertilization and single-cell RNA sequencing, and both indicated an improvement in oocyte developmental potential. In conclusion, we successfully obtained the first live dogs using in vitro matured oocytes by adding P4 to optimize the in vitro maturation system of canine oocytes, and established a new and low-cost method to produce dogs via in vitro maturation and in vitro fertilization.<\/jats:p>","DOI":"10.3390\/life12111778","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T04:00:51Z","timestamp":1667534451000},"page":"1778","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Progesterone Promotes In Vitro Maturation of Domestic Dog Oocytes Leading to Successful Live Births"],"prefix":"10.3390","volume":"12","author":[{"given":"Yumin","family":"Qin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenjiong","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"Beijing SINOGENE Biotechnology Co., Ltd., Beijing 102200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing SINOGENE Biotechnology Co., Ltd., Beijing 102200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing SINOGENE Biotechnology Co., Ltd., Beijing 102200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qintao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Nanchang Police-dog Base of the Ministry of Public Security of PRC, Nanchang 330100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhua","family":"Ye","sequence":"additional","affiliation":[{"name":"Nanchang Police-dog Base of the Ministry of Public Security of PRC, Nanchang 330100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jidong","family":"Mi","sequence":"additional","affiliation":[{"name":"Beijing SINOGENE Biotechnology Co., Ltd., Beijing 102200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yougang","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.15302\/J-FASE-2019309","article-title":"A brief review of genome editing technology for generating animal models","volume":"7","author":"Wang","year":"2020","journal-title":"Front. 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