{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:41:07Z","timestamp":1785739267382,"version":"3.56.0"},"reference-count":78,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DBT-RA program","award":["DBT\/2020\/January\/NE14"],"award-info":[{"award-number":["DBT\/2020\/January\/NE14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Aeromonas species exhibit widespread presence in food, poultry, and aquaculture. They are major multi-drug-resistant fish pathogens. This study aims to identify Aeromonas species harbouring virulence genes aerolysin, flagellin, and lipase from diseased fishes of Assam wetlands with association with antibiotic resistance and in vivo pathogenicity. One hundred and thirty-four Aeromonas strains were isolated and thirty representative species identified using genus-specific 16S rRNA gene amplification. A. veronii was most prevalent (53.7%) followed by A. hydrophila (40.2%), A. caviae (4.47%), and A. dhakensis (1.49%). Ninety percent (90%) of strains harboured at least one of the studied virulence genes: aerA (73.3%), lip (46.6%), and flaA (26.6%). The highest multiple antibiotic resistance (MAR) index 0.8 corresponded to A. hydrophila DBTNE1 (MZ723069), containing all the studied genes. The lowest LD50 values (1.6 \u00d7 106 CFU\/fish) corresponded to isolates having both aerA and lip. \u03b2-lactams showed utmost resistance and lowest for aminoglycosides. There was a significant (p &lt; 0.05) Pearson chi-square test of association between the occurrence of virulence and antibiotic resistance. The in silico protein\u2013protein interaction revealed important drug targets, such as \u03c328 transcription factor, aminoacyl-tRNA synthetase, and diacylglycerol kinase, with significant (p &lt; 0.05) enrichment. This study suggests that fish-isolate Aeromonas strains represent potential threat to aquaculture with subsequent risk of transferring antibiotic resistance to human pathogens.<\/jats:p>","DOI":"10.3390\/life12121979","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T03:28:49Z","timestamp":1669606129000},"page":"1979","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Co-Prevalence of Virulence and Pathogenic Potential in Multiple Antibiotic Resistant Aeromonas spp. from Diseased Fishes with In Silico Insight on the Virulent Protein Network"],"prefix":"10.3390","volume":"12","author":[{"given":"Nabanita","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Regional Centre, Central Inland Fisheries Research Institute (ICAR), Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basanta","family":"Das","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2990-9280","authenticated-orcid":false,"given":"Asit","family":"Bera","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5973-6424","authenticated-orcid":false,"given":"Simanku","family":"Borah","sequence":"additional","affiliation":[{"name":"Regional Centre, Central Inland Fisheries Research Institute (ICAR), Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasmita","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5375-9596","authenticated-orcid":false,"given":"Anil","family":"Yadav","sequence":"additional","affiliation":[{"name":"Regional Centre, Central Inland Fisheries Research Institute (ICAR), Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1319-2788","authenticated-orcid":false,"given":"Jeetendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Regional Centre, Central Inland Fisheries Research Institute (ICAR), Prayagraj 211002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Koushlesh","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangjam","family":"Chanu","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0513-1101","authenticated-orcid":false,"given":"Soumya","family":"Panda","sequence":"additional","affiliation":[{"name":"Central Inland Fisheries Research Institute (ICAR), Barrackpore 700120, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravali","family":"Vallangi","sequence":"additional","affiliation":[{"name":"Regional Centre, Central Inland Fisheries Research Institute (ICAR), Guwahati 781006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3417","DOI":"10.20546\/ijcmas.2018.711.392","article-title":"Trends in Fish Production of Assam: An Analysis","volume":"7","author":"Chutia","year":"2018","journal-title":"Int. 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