{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:18:06Z","timestamp":1789665486458,"version":"4.0.1"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Tsing Hua University","award":["102-2314-B-007-002-MY2"],"award-info":[{"award-number":["102-2314-B-007-002-MY2"]}]},{"name":"National Tsing Hua University","award":["103-2622-B-007-002-CC2"],"award-info":[{"award-number":["103-2622-B-007-002-CC2"]}]},{"name":"Ministry of Science and Technology of Taiwan","award":["102-2314-B-007-002-MY2"],"award-info":[{"award-number":["102-2314-B-007-002-MY2"]}]},{"name":"Ministry of Science and Technology of Taiwan","award":["103-2622-B-007-002-CC2"],"award-info":[{"award-number":["103-2622-B-007-002-CC2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Background: Osteosarcoma (OS) is the most common primary malignancy of the bone and is notoriously resistant to radiation therapy. High-dose cytotoxic chemotherapy and surgical resection have improved the survival rate and prognosis of patients with OS. Nonetheless, treatment challenges remain when the tumor cannot be removed by surgery. Boron neutron capture therapy (BNCT) provides high linear energy transfer (LET) radiation, and its internal targeted characteristics make BNCT a novel therapy for removing OS and reducing radiation damage to adjacent healthy tissues. Methods: In this study, a UMR-106-grafted OS rat model was developed, and boric acid (BA) was used as the boron drug for BNCT. The pharmacokinetics of BA, following intravenous injection, were evaluated to determine the optimal time window for neutron irradiation. OS-bearing rats were irradiated by an epithermal neutron beam at Tsing Hua Open-Pool Reactor (THOR). The therapeutic efficacy of and tissue response after BNCT were evaluated by radiographic and histopathological observations. Results: OS-bearing rats were irradiated by neutrons in the first hour following the intravenous injection of BA. The prescription-absorbed doses in the tumor regions were 5.8 and 11.0 Gy. BNCT reduced the body weight of the tumor-bearing rats, but they recovered after a few days. The BA-mediated BNCT effectively controlled the orthotopic OS tumor, reduced osteolysis, and induced bone healing. Autoradiography and histological analysis confirmed that the BA retention region is consistent with the calcification region in OS tissue. Conclusion: BA is specifically retained in OS, and the BA-mediated BNCT can significantly reduce the tumor burden and osteolysis in OS-bearing rats.<\/jats:p>","DOI":"10.3390\/life13020514","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T05:59:40Z","timestamp":1676267980000},"page":"514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Therapeutic Efficacy and Radiobiological Effects of Boric-Acid-Mediated BNCT in an Osteosarcoma-Bearing SD Rat Model"],"prefix":"10.3390","volume":"13","author":[{"given":"Chen-Fang","family":"Hsu","sequence":"first","affiliation":[{"name":"Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9196-4897","authenticated-orcid":false,"given":"Jinn-Jer","family":"Peir","sequence":"additional","affiliation":[{"name":"Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7374-1203","authenticated-orcid":false,"given":"Jiunn-Wang","family":"Liao","sequence":"additional","affiliation":[{"name":"Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3191-1563","authenticated-orcid":false,"given":"Kuan-Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Veterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oncology, Taipei Veterans General Hospital, Taipei 11217, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6022-0107","authenticated-orcid":false,"given":"Yung-Jen","family":"Chuang","sequence":"additional","affiliation":[{"name":"School of Medicine, National Tsing Hua University, Hsinchu 30013, Taiwan"},{"name":"Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fong-In","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu 30013, Taiwan"},{"name":"Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1309\/UC6KQHLD9LV2KENN","article-title":"Osteosarcoma: Anatomic and histologic variants","volume":"125","author":"Klein","year":"2006","journal-title":"Am. 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