{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T14:37:38Z","timestamp":1777041458827,"version":"3.51.4"},"reference-count":25,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["NV19-05-00191"],"award-info":[{"award-number":["NV19-05-00191"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["IGA_LF_2022_011"],"award-info":[{"award-number":["IGA_LF_2022_011"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Palacky University Olomouc","award":["NV19-05-00191"],"award-info":[{"award-number":["NV19-05-00191"]}]},{"name":"Palacky University Olomouc","award":["IGA_LF_2022_011"],"award-info":[{"award-number":["IGA_LF_2022_011"]}]},{"name":"Palacky University Olomouc","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]},{"name":"Ministry of Education, Youth, and Sport of the Czech Republic","award":["NV19-05-00191"],"award-info":[{"award-number":["NV19-05-00191"]}]},{"name":"Ministry of Education, Youth, and Sport of the Czech Republic","award":["IGA_LF_2022_011"],"award-info":[{"award-number":["IGA_LF_2022_011"]}]},{"name":"Ministry of Education, Youth, and Sport of the Czech Republic","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/0007397"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Most patients suffering from Lyme disease are effectively treated with antibiotics. In some patients, however, problems persist for a long time despite appropriate therapy. The term post-treatment Lyme disease syndrome (PTLDS) is currently used for this condition in scientific literature. The pathogenesis is still not precisely known, but the involvement of immunopathological mechanisms is assumed. In our study, we analyzed the presence of autoantibodies including myositis-specific (MSA) and myositis-associated autoantibodies (MAA) in patients with laboratory proven history of Lyme disease and with clinical symptoms of PTLDS. A total of 59 patients meeting the criteria for PTLDS were enrolled in this study. The control group consisted of 40 patients undergoing differential diagnosis of neurological disorders without clinical and\/or laboratory-proven history of Lyme disease. The presence of autoantibodies was determined by immunoblot methods and positive samples were further tested for serum creatine kinase (CK) and myoglobin levels. The presence of myositis autoantibodies was detected in 18 subjects with suspected PTLDS (30.5%), but only in 5% of control subjects exhibiting no evidence of Lyme disease history. The difference was statistically significant (p = 0.002). The subsequent biochemical analysis of muscle-damage markers in positive subjects found a mild elevation in six MSA\/MAA-positive PTLDS patients. The study detected raised MSA\/MAA autoantibodies formation in the group of PTLDS patients raising the question about their involvement in the pathogenesis of this syndrome.<\/jats:p>","DOI":"10.3390\/life13020527","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T03:09:21Z","timestamp":1676430561000},"page":"527","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Myositis Autoantibodies in Patients with Suspected Post-Treatment Lyme Disease Syndrome"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0060-5670","authenticated-orcid":false,"given":"Kristyna","family":"Sloupenska","sequence":"first","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora","family":"Koubkova","sequence":"additional","affiliation":[{"name":"Department of Allergology and Clinical Immunology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2394-064X","authenticated-orcid":false,"given":"Pavel","family":"Horak","sequence":"additional","affiliation":[{"name":"Third Department of Internal Medicine-Nephrology, Rheumatology and Endocrinology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Hutyrova","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"},{"name":"Department of Allergology and Clinical Immunology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojmir","family":"Racansky","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"},{"name":"Department of Allergology and Clinical Immunology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Mares","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Miklusova","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Schovanek","sequence":"additional","affiliation":[{"name":"Third Department of Internal Medicine-Nephrology, Rheumatology and Endocrinology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Zapletalova","sequence":"additional","affiliation":[{"name":"Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8572-7246","authenticated-orcid":false,"given":"Milan","family":"Raska","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"},{"name":"Department of Immunology, University Hospital Olomouc, Zdravotniku 248\/7, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9699-7903","authenticated-orcid":false,"given":"Michal","family":"Krupka","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16090","DOI":"10.1038\/nrdp.2016.90","article-title":"Lyme borreliosis","volume":"2","author":"Steere","year":"2016","journal-title":"Nat. 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North Am."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.ttbdis.2011.04.002","article-title":"Updates on Borrelia burgdorferi sensu lato complex with respect to public health","volume":"2","author":"Rudenko","year":"2011","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1051\/parasite\/2008153244","article-title":"Borrelia burgdorferi sensu lato, the agent of lyme borreliosis: Life in the wilds","volume":"15","author":"Gern","year":"2008","journal-title":"Parasite"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s00005-011-0128-z","article-title":"Prevention of lyme disease: Promising research or sisyphean task?","volume":"59","author":"Krupka","year":"2011","journal-title":"Arch. Immunol. Ther. Exp."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.amjmed.2009.05.011","article-title":"Subjective symptoms after treatment of early Lyme disease","volume":"123","author":"Cerar","year":"2010","journal-title":"Am. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1086\/508667","article-title":"The Clinical Assessment, Treatment, and Prevention of Lyme Disease, Human Granulocytic Anaplasmosis, and Babesiosis: Clinical Practice Guidelines by the Infectious Diseases Society of America","volume":"43","author":"Wormser","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","first-page":"w14353","article-title":"Update of the Swiss guidelines on post-treatment Lyme disease syndrome","volume":"146","author":"Nemeth","year":"2016","journal-title":"Swiss Med. Wkly."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.idc.2015.02.012","article-title":"Posttreatment Lyme Disease Syndrome","volume":"29","author":"Aucott","year":"2015","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Shor, S., Green, C., Szantyr, B., Phillips, S., Liegner, K., Burrascano, J., Bransfield, R., and Maloney, E.L. (2019). Chronic Lyme Disease: An Evidence-Based Definition by the ILADS Working Group. Antibiotics, 8.","DOI":"10.3390\/antibiotics8040269"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s13071-019-3495-7","article-title":"Metamorphoses of Lyme disease spirochetes: Phenomenon of Borrelia persisters","volume":"12","author":"Rudenko","year":"2019","journal-title":"Parasit. Vectors"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sloupenska, K., Dolezilkova, J., Koubkova, B., Hutyrova, B., Racansky, M., Horak, P., Golovchenko, M., Raska, M., Rudenko, N., and Krupka, M. (2021). Seroprevalence of antibodies against tick-borne pathogens in czech patients with suspected post-treatment lyme disease syndrome. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112217"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nrrheum.2018.56","article-title":"Autoantibodies in myositis","volume":"14","author":"McHugh","year":"2018","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4078\/jrd.2019.26.3.165","article-title":"The Role of Autoantibodies in Idiopathic Inflammatory Myopathies","volume":"26","author":"Yoo","year":"2019","journal-title":"J. Rheum. Dis."},{"key":"ref_16","first-page":"1","article-title":"Cultivation Methods of spirochetes from Borrelia burgdorferi sensu lato complex and relapsing fever Borrelia","volume":"2022","author":"Berthold","year":"2022","journal-title":"J. Vis. Exp."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s12016-016-8564-5","article-title":"The clinical relevance of anti-DFS70 Autoantibodies","volume":"52","author":"Conrad","year":"2017","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1093\/brain\/aws106","article-title":"Inflammatory myopathies associated with anti-mitochondrial antibodies","volume":"135","author":"Maeda","year":"2012","journal-title":"Brain"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/BF00838161","article-title":"Borrelia burgdorferi myositis: Report of eight patients","volume":"240","author":"Reimers","year":"1993","journal-title":"J. Neurol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nilsson, K., Skoog, E., Jones, V., Labb\u00e9 Sandelin, L., Bj\u00f6rling, C., Fridenstr\u00f6m, E., Edvinsson, M., M\u00e5rtensson, A., and Olsen, B. (2021). A comprehensive clinical and laboratory evaluation of 224 patients with persistent symptoms attributed to presumed tick-bite exposure. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0247384"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.pathol.2016.03.012","article-title":"Diagnostic performance of a commercial immunoblot assay for myositis antibody testing","volume":"48","author":"Bundell","year":"2016","journal-title":"Pathology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.autrev.2009.03.005","article-title":"Use of a commercial line blot assay as a screening test for autoantibodies in inflammatory myopathies","volume":"9","author":"Storfors","year":"2009","journal-title":"Autoimmun. Rev."},{"key":"ref_23","first-page":"152","article-title":"Antinuclear antibodies in patients with Lyme disease","volume":"4","author":"Gajownik","year":"2010","journal-title":"New Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1080\/08916930410001713098","article-title":"Molecular mimicry to Borrelia burgdorferi: Pathway to autoimmunity?","volume":"37","author":"Bolz","year":"2004","journal-title":"Autoimmunity"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5417","DOI":"10.1038\/s41467-021-25509-3","article-title":"New-onset IgG autoantibodies in hospitalized patients with COVID-19","volume":"12","author":"Chang","year":"2021","journal-title":"Nat. Commun."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/2\/527\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:35:54Z","timestamp":1760121354000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/2\/527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,15]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["life13020527"],"URL":"https:\/\/doi.org\/10.3390\/life13020527","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,15]]}}}