{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:52:55Z","timestamp":1787028775907,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"VEKOP","award":["2.3.2-16-2016-00012"],"award-info":[{"award-number":["2.3.2-16-2016-00012"]}]},{"name":"VEKOP","award":["2019-2.1.11-T\u00c9T-2019-00036"],"award-info":[{"award-number":["2019-2.1.11-T\u00c9T-2019-00036"]}]},{"name":"VEKOP","award":["RRF-2.3.1-21-2022-00007"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Recently, in vitro gene preservation has gained ground thanks to its lower cost and higher stability compared to in vivo techniques. One of the methods that can preserve female-specific W chromosome-linked genes is primordial germ cell (PGC) freezing. PGCs can be isolated from Hamburger\u2013Hamilton stage 14\u201316 embryos via blood sampling. In our experiment, we used two newly established Black Transylvanian naked neck chicken cell lines and four cell lines from our gene bank. We compared two different freezing media (FAM1 and FAM2) in this study. The cell number and viability of the PGCs were measured before freezing (BF) and after thawing on Day 0, Day 1, and Day 7 of cultivation. We analyzed the germ cell-specific chicken vasa homologue (CVH) expression profile in PGCs using RT-qPCR. We found that on Day 0, immediately after thawing, the cell number in cell lines frozen with the FAM2 medium was significantly higher than in the FAM1-treated ones. On Day 1 and Day 7, the cell number and viability were also higher in most cell lines frozen with FAM2, but the difference was insignificant. The freezing also affected the chicken vasa homologue gene expression in male lines treated with both freezing media.<\/jats:p>","DOI":"10.3390\/life13040867","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T04:31:05Z","timestamp":1679632265000},"page":"867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8636-3624","authenticated-orcid":false,"given":"Andr\u00e1s","family":"Ecker","sequence":"first","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0716-6113","authenticated-orcid":false,"given":"Bence","family":"L\u00e1z\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"National Centre for Biodiversity and Gene Conservation, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8917-9785","authenticated-orcid":false,"given":"Roland Imre","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Urb\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolett","family":"Tokodyn\u00e9 Szabadi","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Salinas Aponte","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1574-7317","authenticated-orcid":false,"given":"Mohd","family":"Adnan","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0114-5255","authenticated-orcid":false,"given":"Eszter","family":"V\u00e1rkonyi","sequence":"additional","affiliation":[{"name":"National Centre for Biodiversity and Gene Conservation, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elen","family":"G\u00f3cza","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 G\u00f6d\u00f6ll\u0151, Hungary"},{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, 2100 G\u00f6d\u00f6ll\u0151, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,23]]},"reference":[{"key":"ref_1","unstructured":"Sumner, J.H. (2018, January 9\u201311). Incredible goals of the world\u2019s poultry industry. Proceedings of the International Poultry Day Conference, Budapest, Hungary."},{"key":"ref_2","first-page":"621","article-title":"Save what can be saved-new possibilities in in vitro gene preservation of poultry species (in Hungarian)","volume":"138","author":"Barna","year":"2016","journal-title":"Hungarian Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod.113.108951","article-title":"Production of live offspring from testicular tissue cryopreserved by vitrification procedures in Japanese quail (Coturnix japonica)","volume":"88","author":"Liu","year":"2013","journal-title":"Biol. Reprod."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3184\/175815513X13740778695007","article-title":"Fundamental principles of cryobiology and application to ex situ conservation of avian species","volume":"6","author":"Liu","year":"2013","journal-title":"Avian Biol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.3382\/ps.2013-03185","article-title":"Cryoconservation of avian gonads in Canada","volume":"92","author":"Silversides","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1080\/19768354.2014.921644","article-title":"Embryoid bodies formation from chicken primordial germ cells","volume":"19","author":"Xiong","year":"2015","journal-title":"Anim. Cells Syst."},{"key":"ref_7","first-page":"139","article-title":"Avian primordial germ cells are of epiblastic origin","volume":"65","author":"Ginsburg","year":"1981","journal-title":"J. Embryol. Exp. Morphol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/0012-1606(80)90117-7","article-title":"From Cleavage to Primitive Streak Formation: A Complementary Normal Table and a New Look at the First Stages of the Development of the Chick II. Microscopic Anatomy and Cell Population Dynamics","volume":"79","author":"Kochav","year":"1980","journal-title":"Dev. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1093\/ps\/86.10.2182","article-title":"Migration and proliferation of primordial germ cells in the early chicken embryo","volume":"86","author":"Nakamura","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_10","first-page":"53","article-title":"Temporal and spatial aspects of the gradual migration of primordial germ cells from the epiblast into the germinal crescent in the avian embryo","volume":"95","author":"Ginsburg","year":"1986","journal-title":"J. Embryol. Exp. Morphol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1530\/jrf.0.1020321","article-title":"Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring","volume":"102","author":"Naito","year":"1994","journal-title":"J. Reprod. Fertil."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2141\/jpsa.40.1","article-title":"Cryopreservation of avian germline cells and subsequent production of viable offsprings","volume":"40","author":"Naito","year":"2003","journal-title":"J. Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.2141\/jpsa.44.73","article-title":"Cryopreservation of premordial germ cells (PGCs) from White Leghorn embryos using commercial cryoprotectants","volume":"44","author":"Setioko","year":"2007","journal-title":"J. Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2141\/jpsa.010085","article-title":"Viability and functionality of primordial germ cells after freeze-thaw in chickens","volume":"48","author":"Nakamura","year":"2011","journal-title":"J. Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.3382\/ps\/pew133","article-title":"Cryopreservation of specialized chicken lines using cultured primordial germ cells","volume":"95","author":"Nandi","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1093\/ps\/76.5.753","article-title":"Production of Chicken Chimeras from Injection of Frozen-Thawed Blastodermal Cells","volume":"76","author":"Kino","year":"1997","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1538\/expanim.47.215","article-title":"Production of Donor-Derived Offspring by Transfer of Primordial Germ Cells in Japanese Quail","volume":"47","author":"Ono","year":"1998","journal-title":"Exp. Anim."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/(SICI)1097-010X(19980215)280:3<265::AID-JEZ8>3.0.CO;2-L","article-title":"Production of germ-line chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken","volume":"280","author":"Tajima","year":"1998","journal-title":"J. Exp. Zool."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"14246","DOI":"10.1038\/s41598-017-14475-w","article-title":"Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken","volume":"7","author":"Trefil","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"101164","DOI":"10.1016\/j.psj.2021.101164","article-title":"Chicken embryo as a model in epigenetic research","volume":"100","author":"Bednarczyk","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/ps\/85.2.237","article-title":"Avian germplasm preservation: Embryonic stem cells or primordial germ cells?","volume":"85","author":"Petitte","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.anireprosci.2012.08.002","article-title":"Novel needle-in-straw vitrification can effectively preserve the follicle morphology, viability, and vascularization of ovarian tissue in Japanese quail (Coturnix japonica)","volume":"134","author":"Liu","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1262\/jrd.2016-052","article-title":"Poultry genetic resource conservation using primordial germ cells","volume":"62","author":"Nakamura","year":"2016","journal-title":"J. Reprod. Dev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.stemcr.2015.10.008","article-title":"FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal","volume":"5","author":"Whyte","year":"2015","journal-title":"Stem Cell Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kong, L., Qiu, L., Guo, Q., Chen, Y., Zhang, X., Chen, B., Zhang, Y., and Chang, G. (2018). Long-term in vitro culture and preliminary establishment of chicken primordial germ cell lines. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196459"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1080\/0007166031000085382","article-title":"Purification of avian circulating primordial germ cells by Nycodenz density gradient centrifugation","volume":"44","author":"Zhao","year":"2003","journal-title":"Br. Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1046\/j.1365-294x.1998.00389.x","article-title":"A DNA test to sex most birds","volume":"7","author":"Griffiths","year":"1998","journal-title":"Mol. Ecol."},{"key":"ref_28","first-page":"1780679","article-title":"Comparison of the MicroRNA expression profiles of male and female avian primordial germ cell lines","volume":"2018","author":"Anand","year":"2018","journal-title":"Stem Cells Int."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chaipipat, S., Sritabtim, K., Piyasanti, Y., Prukudom, S., Jurutha, J., Phetpila, V., Sinsiri, R., Kammongkun, J., Molee, A., and Thiangtum, K. (Biopreserv. Biobank., 2022). Initiative on Avian Primordial Germ Cell Cryobanking in Thailand, Biopreserv. Biobank., online ahead of print.","DOI":"10.1089\/bio.2022.0043"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/jmor.1050880104","article-title":"A series of normal stages in the development of the chick embryo","volume":"88","author":"Hamburger","year":"1951","journal-title":"J. Morphol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Divya, D., Shukla, R., Chatterjee, R., Sagar, G., Rajendra Prasad, A., and Bhattacharya, T. (2021). Production of Transgenic Chimeric Chicken from Cryopreserved Primordial Germ Cells and its Validation by Developing shRNA Transgenic Chicken Chimera. Res. Sq.","DOI":"10.21203\/rs.3.rs-275932\/v1"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.1242\/dev.127.12.2741","article-title":"Isolationof chicken vasa homolog gene and tracing the origin of primordial germ cells","volume":"127","author":"Tsunekawa","year":"2000","journal-title":"Development"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1387\/ijdb.170283jh","article-title":"The early development of germ cells in chicken","volume":"62","author":"Kim","year":"2018","journal-title":"Int. J. Dev. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"101207","DOI":"10.1016\/j.psj.2021.101207","article-title":"Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells","volume":"100","author":"McGrew","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s10616-020-00385-9","article-title":"An improved protocol for stable and efficient culturing of chicken primordial germ cells using small-molecule inhibitors","volume":"72","author":"Ezaki","year":"2020","journal-title":"Cytotechnology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.morpho.2020.01.003","article-title":"Is Vasa such a highly specific marker for primordial germ cells? A comparison of VASA and HSP90 proteins expression in young chicken embryos","volume":"104","author":"Lejong","year":"2020","journal-title":"Morphologie"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1111\/1755-0998.13381","article-title":"Long-term stability of sex chromosome gene content allows accurate qPCR-based molecular sexing across birds","volume":"21","author":"Mazzoleni","year":"2021","journal-title":"Mol. Ecol."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/4\/867\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:01:52Z","timestamp":1760122912000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/4\/867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,23]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["life13040867"],"URL":"https:\/\/doi.org\/10.3390\/life13040867","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,23]]}}}