{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:32:43Z","timestamp":1767918763950,"version":"3.49.0"},"reference-count":36,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Institute of Pharmaceutical Sciences, Panjab University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The current study was undertaken to evaluate the efficacy of a novel nano-lipoidal eye drop formulation of triamcinolone acetonide (TA) for the topical treatment of uveitis. The triamcinolone acetonide-loaded nanostructured lipid carriers (cTA-NLC) were developed by employing \u2018hot microemulsion method\u2019 using biocompatible lipids, which exhibited a sustained release nature and enhanced efficacy when evaluated in vitro. The in vivo efficacy of this developed formulation was tested on Wistar rats, and a single-dose pharmacokinetic study was conducted in rabbits. The eyes of animals were examined for any signs of inflammation using the \u2018Slit-lamp microscopic\u2019 method. The aqueous humor collected from the sacrificed rats was tested for total protein count and cell count. The total protein count was determined using BSA assay method, while the total cell count was determined by Neubaur\u2019s hemocytometer method. The results showed that the cTA-NLC formulation had negligible signs of inflammation, with a clinical score of uveitis 0.82 \u00b1 0.166, which is much less than control\/untreated (3.80 \u00b1 0.3) and free drug suspension (2.66 \u00b1 0.405). The total cell count was also found to be significantly low for cTA-NLC (8.73 \u00b1 1.79 \u00d7 105) as compared to control (52.4 \u00b1 7.71 \u00d7 105) and free drug suspension (30.13 \u00b1 3.021 \u00d7 105). Conclusively, the animal studies conducted showed that our developed formulation holds the potential for effective management of uveitis.<\/jats:p>","DOI":"10.3390\/life13041057","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T04:53:59Z","timestamp":1681966439000},"page":"1057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cationic Nano-Lipidic Carrier Mediated Ocular Delivery of Triamcinolone Acetonide: A Preclinical Investigation in the Management of Uveitis"],"prefix":"10.3390","volume":"13","author":[{"given":"Pradip","family":"Nirbhavane","sequence":"first","affiliation":[{"name":"UGC-Centre of Advanced Study, Division of Pharmaceutics, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4914-9952","authenticated-orcid":false,"given":"Laxmi","family":"Moksha","sequence":"additional","affiliation":[{"name":"Dr. Rajendra Prasad Centre for Ophthalmic Sciences, Ocular Pharmacology and Pharmacy Division, All India Institute of Medical Sciences, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8369-0219","authenticated-orcid":false,"given":"Gajanand","family":"Sharma","sequence":"additional","affiliation":[{"name":"UGC-Centre of Advanced Study, Division of Pharmaceutics, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirumurthy","family":"Velpandian","sequence":"additional","affiliation":[{"name":"Dr. Rajendra Prasad Centre for Ophthalmic Sciences, Ocular Pharmacology and Pharmacy Division, All India Institute of Medical Sciences, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhupinder","family":"Singh","sequence":"additional","affiliation":[{"name":"UGC-Centre of Advanced Study, Division of Pharmaceutics, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2940-2065","authenticated-orcid":false,"given":"O. P.","family":"Katare","sequence":"additional","affiliation":[{"name":"UGC-Centre of Advanced Study, Division of Pharmaceutics, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s13643-016-0203-y","article-title":"The effectiveness of pharmacological agents for the treatment of uveitic macular oedema (UMO): A systematic review protocol","volume":"5","author":"Tallouzi","year":"2016","journal-title":"Syst. 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