{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:05:57Z","timestamp":1790816757542,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN","award":["AGROALNEXT\/2022\/052-BIOPESTNEW"],"award-info":[{"award-number":["AGROALNEXT\/2022\/052-BIOPESTNEW"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The need for alternatives to synthetic pesticides is a priority today, especially when these pesticides are directed against aphids, one of the more challenging pests facing modern agriculture. Essential oils may be one of these alternatives. We assayed the insecticidal potential of essential oils from Thymus vulgaris, Rosmarinus officinalis var. \u2018prostratus\u2019 and Lavandula dentata. Essential oil extraction was carried out by hydrodistillation in a Clevenger-type apparatus for 3 h and their respective composition was elucidated by gas chromatography-mass spectrometry. The essential oil fraction from T. vulgaris contained 81.20% monoterpenoids and 12.85% sesquiterpenoids; R. officinalis var. \u2018prostratus\u2019 contained 91.98% monoterpenoids and 1.93% sesquiterpenoids, while L. dentata contained 69.60% monoterpenoids and 8.05% sesquiterpenoids. The major components found were 1,8-cineole (18.11%), camphor (11.18) and borneol (10.32%) in T. vulgaris; \u03b1-pinene (18.72%), verbenone (13.42%) and 1,8-cineole (10.32%) in R. officinalis; and 1,8-cineole (34.65%), camphor (7.58%) and \u03b2-pinene (6.39%) in L. dentata. The insecticidal activity of the essential oils was evaluated by contact toxicity bioassays against the bird cherry oat aphid, Rhopalosiphum padi L. We observed a mortality rate of 78.3% \u00b1 23.9 at 15 \u03bcL\/mL with T. vulgaris, 54.7% \u00b1 25.8 with L. dentata (although at a lower concentration, 10 \u03bcL\/mL), and 56.7% \u00b1 25.6 at 15 \u03bcL\/mL with R. officinalis. Our results suggest that thyme essential oil may be particularly promising for integrated aphid management provided that specific conditions of use and dosages are observed.<\/jats:p>","DOI":"10.3390\/life13081621","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:45:02Z","timestamp":1690332302000},"page":"1621","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Essential Oils from Selected Mediterranean Aromatic Plants\u2014Characterization and Biological Activity as Aphid Biopesticides"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8929-1331","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Casas","sequence":"first","affiliation":[{"name":"Research Institute CIBIO (Centro Iberoamericano de la Biodiversidad), Scientific Park, University of Alicante, Ctra. San Vicente del Raspeig s\/n, E-03690 San Vicente del Raspeig, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aitor","family":"Sagarduy-Cabrera","sequence":"additional","affiliation":[{"name":"Research Institute CIBIO (Centro Iberoamericano de la Biodiversidad), Scientific Park, University of Alicante, Ctra. San Vicente del Raspeig s\/n, E-03690 San Vicente del Raspeig, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"L\u00f3pez Santos-Olmo","sequence":"additional","affiliation":[{"name":"Research Institute CIBIO (Centro Iberoamericano de la Biodiversidad), Scientific Park, University of Alicante, Ctra. San Vicente del Raspeig s\/n, E-03690 San Vicente del Raspeig, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2439-2630","authenticated-orcid":false,"given":"M\u00aa \u00c1ngeles","family":"Marcos-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Research Institute CIBIO (Centro Iberoamericano de la Biodiversidad), Scientific Park, University of Alicante, Ctra. San Vicente del Raspeig s\/n, E-03690 San Vicente del Raspeig, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref_1","unstructured":"Resh, V.H., and Card\u00e9, R.T. (2009). Encyclopedia of Insects, Academic Press. [2nd ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.plantsci.2018.04.027","article-title":"Plant defense against aphids, the pest extraordinaire","volume":"279","author":"Nalam","year":"2019","journal-title":"Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Sun, M., Voorrips, R.E., Steenhis-Broers, G., Van\u2019t Westende, W., and Vosman, B. (2018). Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession. BMC Plant Biol., 18.","DOI":"10.1186\/s12870-018-1340-3"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.crvi.2010.04.001","article-title":"Aphids as transport devices for plant viruses","volume":"333","author":"Brault","year":"2010","journal-title":"Comptes Rendus Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.crvi.2010.03.009","article-title":"The conflicting relationships between aphids and men: A review of aphid damage and control strategies","volume":"333","author":"Dedryver","year":"2010","journal-title":"Comptes Rendus Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1007\/s10340-019-01089-6","article-title":"Essential oils as active ingredients of botanical insecticides against aphids","volume":"92","author":"Ikbal","year":"2019","journal-title":"J. Pest Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.ento.43.1.1","article-title":"Golden Age of insecticide research","volume":"43","author":"Casida","year":"1998","journal-title":"Annu. Rev. Entomol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ibmb.2014.05.003","article-title":"The evolution of insecticide resistance in the peach potato aphid Myzus persicae","volume":"51","author":"Bass","year":"2014","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1002\/ps.3663","article-title":"Biopesticides in the framework of the European Pesticide Regulation (EC) No. 1107\/2009","volume":"70","author":"Villaverde","year":"2013","journal-title":"Pest Manag. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.biocontrol.2012.06.001","article-title":"Prey availability and abiotic requirements of immature stages of the aphid predator Sphaerophoria rueppellii","volume":"63","author":"Pineda","year":"2012","journal-title":"Biol. Control"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104328","DOI":"10.1016\/j.biocontrol.2020.104328","article-title":"One stone; two birds: Concurrent pest control and pollination services provided by aphidophagous hoverflies","volume":"149","author":"Pekas","year":"2020","journal-title":"Biol. Control"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev-ento-120710-100554","article-title":"Essential oils in insect control: Low-risk products in a high-stakes world","volume":"57","author":"Vincent","year":"2012","journal-title":"Annu. Rev. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"de Sousa, D.P. (2015). Bioactive Essential Oils and Cancer, Springer International Publishing.","DOI":"10.1007\/978-3-319-19144-7"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/ffj.3515","article-title":"Oxygen heterocyclic compound screening in Citrus essential oils by linear retention index approach applied to liquid chromatography coupled to photodiode array detector","volume":"34","author":"Rigano","year":"2019","journal-title":"Flav. Frag. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/j.tplants.2016.10.005","article-title":"Essential oils as eco-friendly biopesticides? Challenges and constraints","volume":"21","author":"Pavela","year":"2016","journal-title":"Trends Plant Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ramawat, K.G., and M\u00e9rillon, J.M. (2013). Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes, Springer.","DOI":"10.1007\/978-3-642-22144-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S0261-2194(00)00079-X","article-title":"Plant essential oils for pest and disease management","volume":"19","author":"Isman","year":"2000","journal-title":"Crop Prot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s11101-017-9512-6","article-title":"Phytochemical biopesticides: Some recent developments","volume":"16","author":"Walia","year":"2017","journal-title":"Phytochem. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/BF02981099","article-title":"Neem and other botanical insecticides: Barriers to commercialization","volume":"25","author":"Isman","year":"1997","journal-title":"Phytoparasitica"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.tplants.2013.11.005","article-title":"Botanical insecticide research: Many publications, limited useful data","volume":"19","author":"Isman","year":"2014","journal-title":"Trends Plant Sci."},{"key":"ref_21","unstructured":"Finney, D.J. (1971). Probit Analysis, Cambridge University Press. [3rd ed.]."},{"key":"ref_22","unstructured":"Mekapogu, A.R. (2023, May 24). Finney\u2019s Probit Analysis Spreadsheet Calculator (Version 2021). Available online: https:\/\/probitanalysis.wordpress.com\/."},{"key":"ref_23","unstructured":"R Core Team (2022). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Novak, J., and Bl\u00fcthner, W.D. (2020). Medicinal Aromatic and Stimulant Plants, Springer International Publishing.","DOI":"10.1007\/978-3-030-38792-1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.indcrop.2006.06.011","article-title":"Seasonal variation of Thymus hyemalis Lange and Spanish Thymus vulgaris L. essential oil composition","volume":"24","author":"Goodner","year":"2006","journal-title":"Ind. Crop Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1002\/ffj.1800","article-title":"Volatile components of Thymus vulgaris L. from wild-growing and cultivated plants in Jordan","volume":"22","author":"Hudaib","year":"2007","journal-title":"Flav. Frag. J."},{"key":"ref_27","first-page":"100797","article-title":"Biological properties and chemical profiling of essential oils of Thymus (vulgaris, algeriensis and broussonettii) grown in Morocco","volume":"37","author":"Labiad","year":"2022","journal-title":"Chem. Data Coll."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1002\/ps.2138","article-title":"Composition and biological activity of essential oils from Labiatae against Nezara viridula (Hemiptera: Pentatomidae) soybean pest","volume":"67","author":"Murray","year":"2011","journal-title":"Pest Manag. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"40902","DOI":"10.1038\/srep40902","article-title":"Insecticidal toxicities of carvacrol and thymol derived from Thymus vulgaris Lin. against Pochazia shantungensis Chou & Lu., newly recorded pest","volume":"7","author":"Park","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.ifset.2007.12.005","article-title":"Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against postharvest fungal infestation of food commodities","volume":"9","author":"Kumar","year":"2008","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10904","DOI":"10.1007\/s11356-018-1398-3","article-title":"Essential oils from Foeniculum vulgare Miller as a safe environmental insecticide against the aphid Myzus persicae Sulzer","volume":"25","author":"Pavela","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s12600-019-00770-x","article-title":"Comparative bioactivity of essential oils from two Mentha pulegium (Lamiaceae) chemotypes against Aphis gossypii, Aphis spiraecola, Tetranychus urticae and the generalist predator Nesidiocoris tenuis","volume":"47","author":"Papadimitriou","year":"2019","journal-title":"Phytoparasitica"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1007\/s42690-022-00766-x","article-title":"Insecticidal activity of the essential oils from yarrow (Achillea wilhelmsii L.) and sweet asafetida (Ferula assa-foetida L.) against Aphis gossypii Glover (Hemiptera: Aphididae) under controlled laboratory conditions","volume":"42","author":"Koorki","year":"2022","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1007\/s11101-021-09745-5","article-title":"Rosemary (Rosmarinus officinalis L.) extraction techniques, analytical methods and health-promoting biological effects","volume":"20","author":"Lesnik","year":"2021","journal-title":"Phytochem. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.tifs.2015.07.015","article-title":"A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.)","volume":"45","author":"Cavaleiro","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s10681-022-03020-0","article-title":"Variability of essential oil in cultivated populations of Rosmarinus officinalis L. in Spain","volume":"218","year":"2022","journal-title":"Euphytica"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Satyal, P., Jones, T.H., Lopez, E.M., McFeeters, R.L., Ali, N.A.A., Mansi, I., Al-kaf, A.G., and Setzer, W.N. (2017). Chemotypic characterization and biological activity of Rosmarinus officinalis. Foods, 6.","DOI":"10.3390\/foods6030020"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ufug.2016.06.029","article-title":"Effect of mulch on initial coverage of four groundcover species for low input landscaping in a Mediterranean climate","volume":"19","author":"Ruggeri","year":"2016","journal-title":"Urban For. Urban Green."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1007\/s10722-020-00939-y","article-title":"Essential oil composition and yield of Rosmarinus officinalis L. natural population with an extended flowering season in a coastal Mediterranean environment and perspectives for exploitations","volume":"67","author":"Serralutzu","year":"2020","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1002\/ffj.1226","article-title":"Extraction of rosemary essential oil by steam distillation and hydrodistillation","volume":"18","author":"Boutekedjiret","year":"2003","journal-title":"Flav. Frag. J."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ahmed, Q., Agarwal, M., Al-Obaidi, R., Wang, P., and Ren, Y. (2021). Evaluation of aphicidal effect of essential oils and their synergistic effect against Myzus persicae (Sulzer) (Hemiptera: Aphididae). Molecules, 26.","DOI":"10.3390\/molecules26103055"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1002\/ps.1157","article-title":"Comparative toxicity of Rosmarinus officinalis L. essential oil and blends of its major constituents against Tetranychus urticae Koch (Acari: Tetranychidae) on two different host plants","volume":"62","author":"Miresmailli","year":"2006","journal-title":"Pest Manag. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.indcrop.2013.04.011","article-title":"Effects of Rosmarinus officinalis and Salvia officinalis essential oils on Tetranychus urticae Koch (Acari: Tetranychidae)","volume":"48","author":"Laborda","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.matpr.2019.04.004","article-title":"Chemical composition and insecticidal activity of five essential oils: Cedrus atlantica, Citrus limonum, Rosmarinus officinalis, Syzygium aromaticum and Eucalyptus globules","volume":"13","author":"Ainane","year":"2019","journal-title":"Mater. Today. Proc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/s11101-017-9517-1","article-title":"Essential oils of Lavanda genus: A systematic review of their chemistry","volume":"16","author":"Aprotosoaie","year":"2017","journal-title":"Phytochem. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.sajb.2023.01.023","article-title":"Traditional use, phytochemistry, toxicology, and pharmacological properties of Lavandula dentata L.: A comprehensive review","volume":"154","author":"Bouyahya","year":"2023","journal-title":"S. Afr. J. Bot."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"102092","DOI":"10.1016\/j.bcab.2021.102092","article-title":"Insecticidal, fungicidal, phytotoxic activity and chemical composition of Lavandula dentata essential oil","volume":"35","author":"Wagner","year":"2021","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1080\/0972060X.2021.1944326","article-title":"Essential oil from flowering tops of Lavandula dentata (L): Chemical composition, antimicrobial, antioxidant and insecticidal activities","volume":"24","author":"Imen","year":"2021","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s11101-010-9170-4","article-title":"Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products","volume":"10","author":"Isman","year":"2011","journal-title":"Phytochem. Rev."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/8\/1621\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:18:47Z","timestamp":1760127527000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/8\/1621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,25]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["life13081621"],"URL":"https:\/\/doi.org\/10.3390\/life13081621","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,25]]}}}