{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:23:33Z","timestamp":1760149413082,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T00:00:00Z","timestamp":1691798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Program for the Promotion and Support of Research Projects","award":["PROFAPI 2022","PRO_A3_023"],"award-info":[{"award-number":["PROFAPI 2022","PRO_A3_023"]}]},{"name":"Autonomous University of Sinaloa","award":["PROFAPI 2022","PRO_A3_023"],"award-info":[{"award-number":["PROFAPI 2022","PRO_A3_023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The search for an animal model to evaluate the allergenic potential of processed food products is still ongoing. Both the sensitization to ovalbumin (OVA) in different structural states and the allergic response triggered after intragastric or food challenges were assessed. BALB\/c mice were sensitized intraperitoneally to OVA (50 \u00b5g) in different structural states (native OVA, N-OVA; denatured OVA, D-OVA; formaldehyde- and lysine-treated OVA, FK-OVA; denatured OVA-FK, OVA-DFK; peptides from pepsin digestion, Pep-OVA). Anti-OVA-specific IgE responses were evaluated using ELISA. Anaphylactic signs and mMCP-1 serum levels were evaluated after intragastric (2.0 mg\/OVA) and food (0.41 mg\/OVA) challenges. IgE reactivities to N-OVA and D-OVA were similar among groups (p &gt; 0.05). After the challenges, all OVA-sensitized mice developed mild to severe anaphylactic signs (p &lt; 0.05 vs. control). Mice sensitized to N-OVA and D-OVA had the highest mMCP-1 serum levels after challenges (p &lt; 0.05 vs. control). Allergic responses were similar despite the different OVA doses used for the challenges. The N-OVA-sensitized murine model of egg allergy proposed in the present study holds the potential for evaluating the impact of food matrix composition and processing on the threshold of egg-allergic responses.<\/jats:p>","DOI":"10.3390\/life13081733","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T10:20:14Z","timestamp":1692008414000},"page":"1733","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Food Matrix Triggers a Similar Allergic Immune Response in BALB\/c Mice Sensitized with Native, Denatured, and Digested Ovalbumin"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6977-5651","authenticated-orcid":false,"given":"Jes\u00fas Gilberto","family":"Ar\u00e1mburo-G\u00e1lvez","sequence":"first","affiliation":[{"name":"Graduate Program in Health Sciences, Faculty of Biological and Health Sciences, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"},{"name":"Nutrition Sciences Graduate Program, Faculty of Nutrition Sciences, Autonomous University of Sinaloa, Culiacan 80019, Sinaloa, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Tinoco-Narez-Gil","sequence":"additional","affiliation":[{"name":"Nutrition Sciences Graduate Program, Faculty of Nutrition Sciences, Autonomous University of Sinaloa, Culiacan 80019, Sinaloa, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo Alejandro","family":"Arvizu-Flores","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, Faculty of Biological and Health Sciences, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4276-8715","authenticated-orcid":false,"given":"Oscar Gerardo","family":"Figueroa-Salcido","sequence":"additional","affiliation":[{"name":"Nutrition Sciences Graduate Program, Faculty of Nutrition Sciences, Autonomous University of Sinaloa, Culiacan 80019, Sinaloa, Mexico"},{"name":"Integral Postgraduate Program in Biotechnology, Faculty of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan 80010, Sinaloa, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Mora-Melgem","sequence":"additional","affiliation":[{"name":"Nutrition Sciences Graduate Program, Faculty of Nutrition Sciences, Autonomous University of Sinaloa, Culiacan 80019, Sinaloa, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alma Rosa","family":"Islas-Rubio","sequence":"additional","affiliation":[{"name":"Department of Plant-Origin Food Technology, Research Center for Food and Development, CIAD, A.C. Carretera Gustavo Enrique Astiazar\u00e1n Rosas, No. 46 Col. La Victoria, Hermosillo 83304, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian Karem","family":"Flores-Mendoza","sequence":"additional","affiliation":[{"name":"Clinical and Research Laboratory (LACIUS, C.N.), Department of Chemical, Biological, and Agricultural Sciences (DC-QB), Faculty of Biological and Health Sciences, University of Sonora, Navojoa 85880, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-4911","authenticated-orcid":false,"given":"Veronica","family":"Lopez-Teros","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, Faculty of Biological and Health Sciences, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2452-0057","authenticated-orcid":false,"given":"Humberto","family":"Astiazaran-Garcia","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, Faculty of Biological and Health Sciences, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"},{"name":"Department of Nutrition, Research Center for Food and Development, CIAD, A.C. Carretera Gustavo Enrique Astiazar\u00e1n Rosas, No. 46 Col. La Victoria, Hermosillo 83304, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4434-0459","authenticated-orcid":false,"given":"Feliznando Isidro","family":"C\u00e1rdenas-Torres","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences, Faculty of Biological and Health Sciences, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8678-0447","authenticated-orcid":false,"given":"No\u00e9","family":"Ontiveros","sequence":"additional","affiliation":[{"name":"Clinical and Research Laboratory (LACIUS, C.N.), Department of Chemical, Biological, and Agricultural Sciences (DC-QB), Faculty of Biological and Health Sciences, University of Sonora, Navojoa 85880, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.tifs.2022.07.009","article-title":"How Does Food Matrix Components Affect Food Allergies, Food Allergens and the Detection of Food Allergens? 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