{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:53:01Z","timestamp":1787028781576,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Agronomic Sciences and Veterinary Medicine of Bucharest","award":["2021-9\/2021.07.19"],"award-info":[{"award-number":["2021-9\/2021.07.19"]}]},{"name":"University of Agronomic Sciences and Veterinary Medicine of Bucharest","award":["CNIF-FDI-2021-0430"],"award-info":[{"award-number":["CNIF-FDI-2021-0430"]}]},{"name":"University of Agronomic Sciences and Veterinary Medicine of Bucharest","award":["CNFIS-FDI-2022-0634"],"award-info":[{"award-number":["CNFIS-FDI-2022-0634"]}]},{"name":"University of Agronomic Sciences and Veterinary Medicine of Bucharest","award":["CNFIS-FDI-2023-F-0715"],"award-info":[{"award-number":["CNFIS-FDI-2023-F-0715"]}]},{"name":"Acronym AgoTromb","award":["2021-9\/2021.07.19"],"award-info":[{"award-number":["2021-9\/2021.07.19"]}]},{"name":"Acronym AgoTromb","award":["CNIF-FDI-2021-0430"],"award-info":[{"award-number":["CNIF-FDI-2021-0430"]}]},{"name":"Acronym AgoTromb","award":["CNFIS-FDI-2022-0634"],"award-info":[{"award-number":["CNFIS-FDI-2022-0634"]}]},{"name":"Acronym AgoTromb","award":["CNFIS-FDI-2023-F-0715"],"award-info":[{"award-number":["CNFIS-FDI-2023-F-0715"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and to characterize it by gross, histological, and immunohistochemical approaches. The investigations have been conducted on 56 domestic carnivores. Fibrin was observed as rosette\u2014like arrangements around platelet aggregates, loose network, wave\u2014like pattern or short fibers and the additional tendency of lines of Zahn being noted inconsistently. All agonal thrombi had positive reactions for anti\u2014CD61 for platelets, disposed in variable\u2014sized clumps or in a linear pattern close to endothelial cells of endocardium. The same positive reaction has been noted to anti\u2014fibrinogen and anti\u2014fibrin antibodies. CD45, CD68 and von Willebrand factor had a very low to absent expression. Cardiac lesions were found in 22 cases (39.29%) suggesting predisposition to agonal thrombosis in animals with cardiovascular diseases. The results prove that agonal thrombus is a third category of blood coagulation that forms strictly during agonal death. The microscopical findings describe the agonal thrombus similar to the morphology of recent thrombus vera. Given the results, the agonal thrombus is a useful tool that confirms the agonal suffering prior to death.<\/jats:p>","DOI":"10.3390\/life13091834","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T09:48:15Z","timestamp":1693388895000},"page":"1834","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Agonal Thrombus at Necropsy\u2014A Third Category of Blood Coagulation in Domestic Carnivores"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2975-0870","authenticated-orcid":false,"given":"Raluca Elena","family":"Tiu","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9566-6216","authenticated-orcid":false,"given":"Raluca Ioana","family":"Rizac","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George Laurentiu","family":"Nicolae","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7680-0326","authenticated-orcid":false,"given":"Raluca Mihaela","family":"Turbatu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3881-6390","authenticated-orcid":false,"given":"Emilia","family":"Ciobotaru-Pirvu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1053\/j.tcam.2012.07.005","article-title":"New Models of Hemostasis","volume":"27","author":"McMichael","year":"2012","journal-title":"Top. 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