{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:06:04Z","timestamp":1788447964167,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 2019\u20131728"],"award-info":[{"award-number":["PICT 2019\u20131728"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 2021-00318"],"award-info":[{"award-number":["PICT 2021-00318"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["20020170100317BA"],"award-info":[{"award-number":["20020170100317BA"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PIBAA 2022"],"award-info":[{"award-number":["PIBAA 2022"]}]},{"name":"Universidad de Buenos Aires","award":["PICT 2019\u20131728"],"award-info":[{"award-number":["PICT 2019\u20131728"]}]},{"name":"Universidad de Buenos Aires","award":["PICT 2021-00318"],"award-info":[{"award-number":["PICT 2021-00318"]}]},{"name":"Universidad de Buenos Aires","award":["20020170100317BA"],"award-info":[{"award-number":["20020170100317BA"]}]},{"name":"Universidad de Buenos Aires","award":["PIBAA 2022"],"award-info":[{"award-number":["PIBAA 2022"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["PICT 2019\u20131728"],"award-info":[{"award-number":["PICT 2019\u20131728"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["PICT 2021-00318"],"award-info":[{"award-number":["PICT 2021-00318"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["20020170100317BA"],"award-info":[{"award-number":["20020170100317BA"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["PIBAA 2022"],"award-info":[{"award-number":["PIBAA 2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Extracellular vesicles released by the primary pathogen of periodontal disease Porphyromonas gingivalis (Pg), referred to as outer membrane vesicles (OMVs), have been associated with the pathogenesis of systemic diseases like cardiovascular disease, rheumatoid arthritis, and Alzheimer\u2019s disease. A pathogenic role for Pg by disrupting placental homeostasis was proposed in the association between periodontal disease and adverse pregnancy outcomes. On the basis that trophoblast-derived factors modulate endothelial and immune cell profiles in normal pregnancy and the scarce presence of Pg in placenta, we hypothesized that OMVs from Pg affect trophoblast cell phenotype, impairing trophoblast\u2013endothelium and trophoblast\u2013neutrophil interactions. By means of in vitro designs with first-trimester human trophoblast cells, endothelial cells, and freshly isolated neutrophils, we showed that Pg OMVs are internalized by trophoblast cells and modulate the activity and expression of functional markers. Trophoblast cells primed with Pg OMVs enhanced neutrophil chemoattraction and lost their anti-inflammatory effect. In addition, reduced migration with enhanced adhesion of monocytes was found in endothelial cells upon incubation with the media from trophoblast cells pretreated with Pg OMVs. Taken together, the results support a pathogenic role of Pg OMVs at early stages of pregnancy and placentation through disruption of trophoblast contribution to vascular transformation and immune homeostasis maintenance.<\/jats:p>","DOI":"10.3390\/life13101971","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T03:49:14Z","timestamp":1695786554000},"page":"1971","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Extracellular Vesicles of Porphyromonas gingivalis Disrupt Trophoblast Cell Interaction with Vascular and Immune Cells in an In Vitro Model of Early Placentation"],"prefix":"10.3390","volume":"13","author":[{"given":"Brenda","family":"Lara","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1341-2813","authenticated-orcid":false,"given":"Mat\u00edas","family":"Sassot","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermina","family":"Calo","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3335-4564","authenticated-orcid":false,"given":"Daniel","family":"Paparini","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gliosca","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"},{"name":"Universidad de Buenos Aires, Facultad de Odontolog\u00eda, C\u00e1tedra de Microbiolog\u00eda, Buenos Aires C1122AAH, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Chaufan","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u00f1aki","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0418-2772","authenticated-orcid":false,"given":"Daiana","family":"Vota","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de Qu\u00edmica Biol\u00f3gica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires C1428EGA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4222-817X","authenticated-orcid":false,"given":"Rosanna","family":"Ramhorst","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires\u2014CONICET, Instituto de 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