{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T11:05:31Z","timestamp":1786791931428,"version":"build-2736575974"},"reference-count":44,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T00:00:00Z","timestamp":1699142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Listerias of the phylogenetic lineage II (PLII) are common in the European environment and are hypovirulent. Despite this, they caused more than a third of the sporadic cases of listeriosis and multi-country foodborne outbreaks. L. monocytogenes ST37 is one of them. During the COVID-19 pandemic, ST37 appeared in clinical cases and ranked second in occurrence among food isolates in the Moscow region. The aim of this study was to describe the genomic features of ST37 isolates from different sources. All clinical cases of ST37 were in the cohort of male patients (age, 48\u201381 years) with meningitis\u2013septicemia manifestation and COVID-19 or Influenza in the anamnesis. The core genomes of the fish isolates were closely related. The clinical and meat isolates revealed a large diversity. Prophages (2\u20134\/genome) were the source of the unique genes. Two clinical isolates displayed pseudolysogeny, and excided prophages were A006-like. In the absence of plasmids, the assortment of virulence factors and resistance determinants in the chromosome corresponded to the hypovirulent characteristics. However, all clinical isolates caused severe disease, with deaths in four cases. Thus, these studies allow us to speculate that a previous viral infection increases human susceptibility to listeriosis.<\/jats:p>","DOI":"10.3390\/life13112167","type":"journal-article","created":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T07:35:06Z","timestamp":1699169706000},"page":"2167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Listeria monocytogenes ST37 Distribution in the Moscow Region and Properties of Clinical and Foodborne Isolates"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7206-3594","authenticated-orcid":false,"given":"Olga L.","family":"Voronina","sequence":"first","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1945-0397","authenticated-orcid":false,"given":"Marina S.","family":"Kunda","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia N.","family":"Ryzhova","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina I.","family":"Aksenova","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita A.","family":"Kustova","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana I.","family":"Karpova","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina R.","family":"Melkumyan","sequence":"additional","affiliation":[{"name":"F.I. Inosemtsev City Clinical Hospital, Fortunatovskaya Str., 1, 105187 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena A.","family":"Klimova","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Epidemiology, A.I. Yevdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Delegatskaya Str., 20, Building 1, 127473 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-1925","authenticated-orcid":false,"given":"Olga A.","family":"Gruzdeva","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Educational Institution of Further Professional Education Russian Medical Academy of Continuous Professional Education, Ministry of Health of the Russian Federation, Barrikadnaya Str., 2\/1, Building 1, 125993 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor S.","family":"Tartakovsky","sequence":"additional","affiliation":[{"name":"N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str., 18, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijmm.2010.05.002","article-title":"Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics","volume":"301","author":"Orsi","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"eabj9805","DOI":"10.1126\/sciadv.abj9805","article-title":"Emergence and global spread of Listeria monocytogenes main clinical clonal complex","volume":"7","author":"Moura","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1038\/s41467-019-10380-0","article-title":"Hypervirulent Listeria monocytogenes clones\u2019 adaption to mammalian gut accounts for their association with dairy products","volume":"10","author":"Maury","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/ng.3501","article-title":"Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity","volume":"48","author":"Maury","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ragon, M., Wirth, T., Hollandt, F., Lavenir, R., Lecuit, M., Le Monnier, A., and Brisse, S. (2008). A new perspective on Listeria monocytogenes evolution. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000146"},{"key":"ref_6","first-page":"e000257","article-title":"LiSEQ-whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe","volume":"5","author":"Painset","year":"2019","journal-title":"Microb. Genom."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5583","DOI":"10.1128\/AEM.01018-14","article-title":"Reservoirs of listeria species in three environmental ecosystems","volume":"80","author":"Linke","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.prevetmed.2007.03.005","article-title":"Listeria monocytogenes fecal shedding in dairy cattle shows high levels of day-to-day variation and includes outbreaks and sporadic cases of shedding of specific L Monocytogenes Subtypes","volume":"80","author":"Ho","year":"2007","journal-title":"Prev. Vet. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Esteban, J.I., Oporto, B., Aduriz, G., Juste, R.A., and Hurtado, A. (2009). Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain. BMC Vet. Res., 5.","DOI":"10.1186\/1746-6148-5-2"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Fredriksson-Ahomaa, M., Sauvala, M., Kurittu, P., Heljanko, V., Heikinheimo, A., and Paulsen, P. (2022). Characterisation of Listeria monocytogenes Isolates from Hunted Game and Game Meat from Finland. Foods, 11.","DOI":"10.3390\/foods11223679"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e0038321","DOI":"10.1128\/mSphere.00383-21","article-title":"Mobile Elements Harboring Heavy Metal and Bacitracin Resistance Genes Are Common among Listeria monocytogenes Strains Persisting on Dairy Farms","volume":"6","author":"Castro","year":"2021","journal-title":"mSphere"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Terentjeva, M., \u0160teingolde, \u017d., Meistere, I., Elferts, D., Avsejenko, J., Streiki\u0161a, M., Gradovska, S., Alksne, L., \u0136ibilds, J., and B\u0113rzi\u0146\u0161, A. (2021). Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia. Pathogens, 10.","DOI":"10.3390\/pathogens10070851"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kubicov\u00e1, Z., Roussel, S., F\u00e9lix, B., and Cabanov\u00e1, L. (2021). Genomic diversity of Listeria monocytogenes isolates from Slovakia (2010 to 2020). Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.729050"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e01253-19","DOI":"10.1128\/AEM.01253-19","article-title":"Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics","volume":"85","author":"Roedel","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Stessl, B., Szakmary-Br\u00e4ndle, K., Vorberg, U., Schoder, D., and Wagner, M. (2020). Temporal analysis of the Listeria monocytogenes population structure in floor drains during reconstruction and expansion of a meat processing plant. Int. J. Food Microbiol., 314.","DOI":"10.1016\/j.ijfoodmicro.2019.108360"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Alvarez-Molina, A., Cobo-D\u00edaz, J.F., L\u00f3pez, M., Prieto, M., de Toro, M., and Alvarez-Ord\u00f3\u00f1ez, A. (2021). Unraveling the emergence and population diversity of Listeria monocytogenes in a newly built meat facility through whole genome sequencing. Int. J. Food Microbiol., 340.","DOI":"10.1016\/j.ijfoodmicro.2021.109043"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Psareva, E.K., Liskova, E.A., Razheva, I.V., Yushina, Y.K., Grudistova, M.A., Gladkova, N.A., Potemkin, E.A., Zhurilov, P.A., Sokolova, E.V., and Andriyanov, P.A. (2021). Diversity of Listeria monocytogenes strains isolated from food products in the Central European part of Russia in 2000\u20132005 and 2019\u20132020. Foods, 10.","DOI":"10.3390\/foods10112790"},{"key":"ref_18","first-page":"33","article-title":"Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic","volume":"23","author":"Voronina","year":"2023","journal-title":"Lond. J. Res. Sci. Nat. Form. (LJRS)"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"W181","DOI":"10.1093\/nar\/gkn179","article-title":"The CGView Server: A comparative genomics tool for circular genomes","volume":"36","author":"Grant","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8365","DOI":"10.1038\/srep08365","article-title":"RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes","volume":"5","author":"Brettin","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"D1020","DOI":"10.1093\/nar\/gkaa1105","article-title":"RefSeq: Expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation","volume":"49","author":"Li","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: A better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Moura, A., Criscuolo, A., Pouseele, H., Maury, M.M., Leclercq, A., Tarr, C., Bj\u00f6rkman, J.T., Dallman, T., Reimer, A., and Enouf, V. (2016). Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes. Nat. Microbiol., 2.","DOI":"10.1038\/nmicrobiol.2016.185"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/10665270050081478","article-title":"A greedy algorithm for aligning DNA sequences","volume":"7","author":"Zhang","year":"2000","journal-title":"J. Comput. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"24373","DOI":"10.1038\/srep24373","article-title":"BPGA- an ultra-fast pan-genome analysis pipeline","volume":"6","author":"Chaudhari","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"W347","DOI":"10.1093\/nar\/gkr485","article-title":"PHAST: A fast phage search tool","volume":"39","author":"Zhou","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"W246","DOI":"10.1093\/nar\/gky425","article-title":"CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins","volume":"46","author":"Couvin","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D325","DOI":"10.1093\/nar\/gki008","article-title":"VFDB: A reference database for bacterial virulence factors","volume":"33","author":"Chen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D690","DOI":"10.1093\/nar\/gkac920","article-title":"CARD 2023: Expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database","volume":"51","author":"Alcock","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kuenne, C., Billion, A., Mraheil, M.A., Strittmatter, A., Daniel, R., Goesmann, A., Barbuddhe, S., Hain, T., and Chakraborty, T. (2013). Reassessment of the Listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-47"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7405","DOI":"10.1128\/JB.00758-06","article-title":"Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes","volume":"188","author":"Hain","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Fox, E.M., Allnutt, T., Bradbury, M.I., Fanning, S., and Chandry, P.S. (2016). Comparative Genomics of the Listeria monocytogenes ST204 Subgroup. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.02057"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7206","DOI":"10.1128\/JB.01041-09","article-title":"Comparative genome analysis of Listeria bacteriophages reveals extensive mosaicism, programmed translational frameshifting, and a novel prophage insertion site","volume":"191","author":"Dorscht","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1099\/00221287-143-6-2065","article-title":"The role of pseudolysogeny in bacteriophage-host interactions in a natural freshwater environment","volume":"143","author":"Ripp","year":"1997","journal-title":"Microbiology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1099\/00221287-144-8-2225","article-title":"Dynamics of the pseudolysogenic response in slowly growing cells of Pseudomonas aeruginosa","volume":"144","author":"Ripp","year":"1998","journal-title":"Microbiology (Reading)"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"M\u00e4ntynen, S., Laanto, E., Oksanen, H.M., Poranen, M.M., and D\u00edaz-Mu\u00f1oz, S.L. (2021). Black box of phage-bacterium interactions: Exploring alternative phage infection strategies. Open Biol., 11.","DOI":"10.1098\/rsob.210188"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/CMR.10.2.345","article-title":"Management of listeriosis","volume":"10","author":"Hof","year":"1997","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_39","unstructured":"Lassen, B., Olsen, A., Sandberg, M., M\u00fcller, L., Torpdahl, M., and Petersen, C.K. (2023). Annual Report on Zoonoses in Denmark 2022, National Food Institute, Technical University of Denmark. Annual Report on Zoonoses in Denmark."},{"key":"ref_40","unstructured":"European Centre for Disease Prevention and Control (2022). ECDC. Annual Epidemiological Report for 2021, ECDC."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Cabal, A., Pietzka, A., Huhulescu, S., Allerberger, F., Ruppitsch, W., and Schmid, D. (2019). Isolate-Based Surveillance of Listeria monocytogenes by Whole Genome Sequencing in Austria. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02282"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ma\u0107kiw, E., Korsak, D., Kowalska, J., Felix, B., Stasiak, M., Kucharek, K., Antoszewska, A., and Postupolski, J. (2021). Genetic diversity of Listeria monocytogenes isolated from ready-to-eat food products in retail in Poland. Int. J. Food Microbiol., 358.","DOI":"10.1016\/j.ijfoodmicro.2021.109397"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Hain, T., Ghai, R., Billion, A., Kuenne, C.T., Steinweg, C., Izar, B., Mohamed, W., Mraheil, M.A., Domann, E., and Schaffrath, S. (2012). Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-144"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cell.2012.06.036","article-title":"Prophage excision activates Listeria competence genes that promote phagosomal escape and virulence","volume":"150","author":"Rabinovich","year":"2012","journal-title":"Cell"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/11\/2167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:17:51Z","timestamp":1760131071000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/13\/11\/2167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,5]]},"references-count":44,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["life13112167"],"URL":"https:\/\/doi.org\/10.3390\/life13112167","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,5]]}}}