{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:12:59Z","timestamp":1762254779694,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Program of Shaanxi Academy of Forestry","award":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"],"award-info":[{"award-number":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"]}]},{"DOI":"10.13039\/501100017592","name":"Science and Technology Program of Shaanxi Province","doi-asserted-by":"publisher","award":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"],"award-info":[{"award-number":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"]}],"id":[{"id":"10.13039\/501100017592","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Xi\u2019an Science and Technology Project","award":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"],"award-info":[{"award-number":["SXLK2021-0213","2022GD-TSLD-57","22NYYF018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) has caused serious damage on jujube trees (Ziziphus jujuba Mill) in northern China. Semiochemicals emerging from the host are essential in the process of insects identifying and localizing the host. The highly expressed odorant-binding protein 1 of S. velatus (SvelOBP1) was assumed to play a possible role in the recognition of host volatiles. In this study, SvelOBP1 was cloned based on the antennal transcriptome of S. velatus. The recombinant SvelOBP1 protein was expressed in Escherichia coli and purified by Ni-NTA resin. The predicted protein SvelOBP1 belonged to a classic OBP subfamily. The expression patterns revealed that SvelOBP1 was mainly expressed in the antennae of both males and females, whereas the expression of SvelOBP1 in other body parts could be neglected. The fluorescence binding assay indicated that SvelOBP1 displayed very strong binding affinities to dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol (Ki = 6.66 \u00b1 0.03 and 7.98 \u00b1 0.06 \u03bcM). The molecular docking results showed that residues Trp114, Phe115 and Asp110 may be involved in binding to both dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol and may have a great impact on odorant recognition of S. velatus. Our results provide evidence that SvelOBP1 might participate in the olfactory molecular perception of S. velatus and would promote the development of pest attractants for S. velatus control.<\/jats:p>","DOI":"10.3390\/life14020192","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T08:11:27Z","timestamp":1706515887000},"page":"192","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6587-9833","authenticated-orcid":false,"given":"Yingyan","family":"Zhai","sequence":"first","affiliation":[{"name":"Shaanxi Key Laboratory of Qinling Ecological Security, Bio-Agriculture Institute of Shaanxi, Shaanxi Academy of Sciences, Xi\u2019an 710043, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Qinling Ecological Security, Bio-Agriculture Institute of Shaanxi, Shaanxi Academy of Sciences, Xi\u2019an 710043, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4206-4275","authenticated-orcid":false,"given":"Tianqi","family":"Tian","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Qinling Ecological Security, Bio-Agriculture Institute of Shaanxi, Shaanxi Academy of Sciences, Xi\u2019an 710043, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwei","family":"Yang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Qinling Ecological Security, Bio-Agriculture Institute of Shaanxi, Shaanxi Academy of Sciences, Xi\u2019an 710043, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Chang\u2019an University Journal Center, Chang\u2019an University, Xi\u2019an 710064, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bowen","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute of Forest Protection, Shaanxi Academy of Forestry, Xi\u2019an 710016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-5562","authenticated-orcid":false,"given":"Bo","family":"Hong","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Qinling Ecological Security, Bio-Agriculture Institute of Shaanxi, Shaanxi Academy of Sciences, Xi\u2019an 710043, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref_1","unstructured":"Zhao, Y.C., and Chen, Y.Q. (1980). Economic Insect Fauna of China: Coleoptera, Curculionidae(\u0399), Science Press."},{"key":"ref_2","first-page":"105","article-title":"Biological characteristics and control of Sympiezomias velatus Chevrolat in Liaoning Province","volume":"7","author":"Yi","year":"2015","journal-title":"Prot. For. Sci. Technol."},{"key":"ref_3","first-page":"33","article-title":"Integrated pest and disease control technology for pollution-free ecological jujube orchards","volume":"10","author":"Qu","year":"2008","journal-title":"Parct. Forest. Tech."},{"key":"ref_4","first-page":"68","article-title":"Experiment on the control effect of Sympiezomias velatus","volume":"6","author":"Wang","year":"2022","journal-title":"Mod. Agri. Sci. Tech."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1007\/s10886-008-9485-4","article-title":"Odor detection in insects: Volatile codes","volume":"34","author":"Bruyne","year":"2008","journal-title":"J. Chem. Ecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/j.ijbiomac.2022.10.197","article-title":"Identification and functional analysis of odorant-binding proteins provide new control strategies for Apolygus lucorum","volume":"224","author":"Liu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/hdy.2009.55","article-title":"Molecular Evolution of the Major Chemosensory Gene Families in Insects","volume":"103","author":"Vieira","year":"2009","journal-title":"Heredity"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0092-8674(00)80582-6","article-title":"A Spatial Map of Olfactory Receptor Expression in the Drosophila Antenna","volume":"96","author":"Vosshall","year":"1999","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.cell.2008.12.001","article-title":"Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila","volume":"136","author":"Benton","year":"2009","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1093\/jee\/tow098","article-title":"Expression of a sensory neuron membrane protein SNMP2 in olfactory sensilla of codling moth cydia pomonella (Lepidoptera: Tortricidae)","volume":"109","author":"Huang","year":"2016","journal-title":"J. Econ. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF00319112","article-title":"Immunolocalization of pheromone-binding protein and general odorant-binding protein in olfactory sensilla of the silk moths Antheraea and Bombyx","volume":"282","author":"Steinbrecht","year":"1995","journal-title":"Cell Tissue Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-ento-120811-153635","article-title":"Odorant Reception in Insects: Roles of Receptors, Binding Proteins, and Degrading Enzymes","volume":"58","author":"Leal","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1007\/s00018-005-5607-0","article-title":"Soluble proteins in insect chemical communication","volume":"63","author":"Pelosi","year":"2006","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jinsphys.2016.09.008","article-title":"A look inside odorant-binding proteins in insect chemoreception","volume":"95","author":"Brito","year":"2016","journal-title":"J. Insect Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1002\/ps.6606","article-title":"Ligand-binding properties of odorant-binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles","volume":"78","author":"Li","year":"2022","journal-title":"Pest. Manag. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1101\/gr.239402","article-title":"Genome-wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster","volume":"12","author":"Scafe","year":"2002","journal-title":"Genome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0083-6729(10)83010-9","article-title":"Odorant-binding proteins in insects","volume":"83","author":"Zhou","year":"2010","journal-title":"Vitam. Horm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/293161a0","article-title":"Pheromone Binding and Inactivation by Moth Antennae","volume":"293","author":"Vogt","year":"1981","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"20000","DOI":"10.1021\/acs.jafc.3c07144","article-title":"An Antennae-Enriched Odorant-Binding Protein EonuOBP43 Mediate the Behavioral Response of the Tea Green Leafhopper, Empoasca onukii Matsuda to the Host and Nonhost Volatiles","volume":"71","author":"Lun","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","first-page":"523","article-title":"Research progress of olfactory binding proteins in insects","volume":"64","author":"Wu","year":"2021","journal-title":"Aata Entomol. Sin."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"924750","DOI":"10.3389\/fphys.2022.924750","article-title":"Insights Into Chemosensory Proteins From Non-Model Insects: Advances and Perspectives in the Context of Pest Management","volume":"13","author":"Lizana","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1093\/jee\/toy066","article-title":"Expression Profiles and Functional Characterization of Two Odorant-Binding Proteins from the Apple Buprestid Beetle Agrilus mali (Coleoptera: Buprestidae)","volume":"111","author":"Cui","year":"2018","journal-title":"J. Econ. Entomol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/1744-7917.12919","article-title":"The Adelphocoris lineolatus OBP4: Support for evolutionary and functional divergence of a mirid pheromone-binding protein from that found in lepidopteran moths","volume":"29","author":"Wang","year":"2022","journal-title":"Insect Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/jisesa\/iead004","article-title":"Odorant-binding Protein 10 From Bradysia odoriphaga (Diptera: Sciaridae) Binds Volatile Host Plant Compounds","volume":"23","author":"Zhu","year":"2023","journal-title":"J. Insect Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Jia, C., Mohamed, A., Cattaneo, A.M., Huang, X.H., Keyhani, N.O., Gu, M.Q., Zang, L.S., and Zhang, W. (2023). Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065595"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"795994","DOI":"10.3389\/fevo.2021.795994","article-title":"Chemosensory Receptors in the Larval Maxilla of Papilio hospiton","volume":"9","author":"Crava","year":"2022","journal-title":"Front. Ecol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S1074-5521(00)00078-8","article-title":"Sexual attraction in the silkworm moth: Structure of the pheromone-binding-protein-bombykol complex","volume":"7","author":"Sandler","year":"2000","journal-title":"Chem. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Leite, N.R., Krogh, R., Xu, W., Ishida, Y., Iulek, J., Leal, W.S., and Oliva, G. (2009). Structure of an odorant-binding protein from the mosquito Aedes aegypti suggests a binding pocket covered by a pH-sensitive \u201clid\u201d. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0008006"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1111\/imb.12179","article-title":"Three amino acid residues of an odorant-binding protein are involved in binding odours in Loxostege sticticalis","volume":"24","author":"Yin","year":"2015","journal-title":"Insect Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.3389\/fphys.2018.01163","article-title":"Odorant receptors and odorant-binding proteins as insect pest control targets: A comparative analysis","volume":"9","author":"Venthur","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"907667","DOI":"10.3389\/fphys.2022.907667","article-title":"Candidate Chemosensory Genes Identified in the Adult Antennae of Sympiezomias velatus and Binding Property of Odorant-Binding Protein 15","volume":"13","author":"Li","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"900752","DOI":"10.3389\/fphys.2022.900752","article-title":"Functional Characterization of Odorant Binding Protein PyasOBP2 From the Jujube Bud Weevil, Pachyrhinus yasumatsui (Coleoptera: Curculionidae)","volume":"13","author":"Hong","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Shen, C., Xia, D.S., Wang, J., and Tang, Q.F. (2019). Characterization of the Expression and Functions of Two Odorant-Binding Proteins of Sitophilus zeamais Motschulsky (Coleoptera: Curculionoidea). Insects, 10.","DOI":"10.3390\/insects10110409"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/plas.1996.0001","article-title":"Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria","volume":"35","author":"Metcalf","year":"1996","journal-title":"Plasmid"},{"key":"ref_35","unstructured":"Promega, and Wagner, T. (2023, March 26). PCR Cloning with Blue\/White Selection and Easy Insert Excision. Available online: https:\/\/www.protocols.io\/view\/pcr-cloning-with-blue-white-selection-and-easy-ins-t89erz6.html."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394Ct Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10797","DOI":"10.1021\/acs.jafc.1c02807","article-title":"Involvement of Chemosensory Protein BodoCSP1 in Perception ofHost Plant Volatiles in Bradysia odoriphaga","volume":"69","author":"Zhang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1068543","DOI":"10.3389\/fphys.2022.1068543","article-title":"Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii","volume":"13","author":"Zhang","year":"2023","journal-title":"Front. Physiol."},{"key":"ref_39","first-page":"621","article-title":"EAG and Olfactory Behavioral Responses of Scythropus yasumatsui to Volatiles from the Zizyphus jujube","volume":"54","author":"Yan","year":"2017","journal-title":"Chin. J. Appl. Entomol."},{"key":"ref_40","first-page":"981","article-title":"EAG and Behavioral Responses of Scythropus yasumatsui (Coleoptera: Curculionidae) to Volatiles from the Common Jujube (Zizyphus jujuba)","volume":"63","author":"Yan","year":"2020","journal-title":"Acta Entomol. Sin."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/j.ijbiomac.2022.11.186","article-title":"Functional disparity of four pheromone-binding proteins from the plum fruit moth Grapholita funebrana Treitscheke in detection of sex pheromone components","volume":"225","author":"Li","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Zhou, X., Zheng, W., Cui, G.C., Du, Z.M., Qian, Y.L., Yang, S.M., Liu, M.H., and Guo, J.X. (2022). Binding Properties of Odorant-Binding Protein 4 of Tirathaba rufivena to Areca catechu Volatiles. Plants, 11.","DOI":"10.3390\/plants11020167"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8388","DOI":"10.1021\/ja204075s","article-title":"Computer Review of Chemdraw Ultra 12.0","volume":"133","author":"Cousins","year":"2011","journal-title":"J. Am. Chem. Soc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1007\/s10340-019-01188-4","article-title":"Identification of odorant-binding proteins and functional analysis of antenna-specific AplaOBP1 in the emerald ash borer, Agrilus planipennis","volume":"93","author":"Wang","year":"2020","journal-title":"J. Pest. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.aspen.2017.02.015","article-title":"Two general odorant binding proteins display high bindings to both host plant volatiles and sex pheromones in a pyralid moth Chilo suppressalis (Lepidoptera: Pyralidae)","volume":"20","author":"Khuhro","year":"2017","journal-title":"J. Asia Pac. Entomol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ibmb.2018.05.003","article-title":"Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon","volume":"98","author":"Huang","year":"2018","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_47","first-page":"1844","article-title":"Oviposition preference of the meadow moth, Loxostege sticticalis L., on different host plants and its chemical mechanism","volume":"25","author":"Yin","year":"2005","journal-title":"Acta Ecol. Sin."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Li, E.T., Wu, H.J., Qin, J.H., Luo, J., Li, K.B., Gao, Y.Z., Zhang, S., Peng, Y., and Yin, J. (2023). Involvement of Holotrichia parallela odorant-binding protein 3 in the localization of oviposition sites. Int. J. Biol. Macromol., 242.","DOI":"10.1016\/j.ijbiomac.2023.124744"},{"key":"ref_49","first-page":"585","article-title":"Feeding preference and taxis behavior of adult Holotrichia oblita (Coleoptera: Scarabaeidae) on three plants","volume":"61","author":"Abdukerim","year":"2018","journal-title":"Acta Entomol. Sin."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1111\/imb.12703","article-title":"Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles","volume":"30","author":"Wei","year":"2021","journal-title":"Insect Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"104012","DOI":"10.1016\/j.jinsphys.2020.104012","article-title":"Sensilla localization and sex pheromone recognition of odorant binding protein OBP4 in the mirid plant bug Adelphocoris lineolatus (Goeze)","volume":"121","author":"Wang","year":"2020","journal-title":"J. Insect Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/imb.12143","article-title":"Two subclasses of odorant-binding proteins in Spodoptera exigua display structural conservation and functional divergence","volume":"24","author":"Liu","year":"2015","journal-title":"Insect Mol. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1111\/brv.12339","article-title":"Beyond chemoreception: Diverse tasks of soluble olfactory proteins in insects","volume":"93","author":"Pelosi","year":"2018","journal-title":"Biol. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"19102","DOI":"10.1073\/pnas.1012274107","article-title":"Crystal and solution structures of an odorant-binding protein from the southern house mosquito complexed with an oviposition pheromone","volume":"107","author":"Mao","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/imb.12088","article-title":"Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann (Coleoptera: Scarabaeida)","volume":"23","author":"Zhuang","year":"2014","journal-title":"Insect Mol. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3068","DOI":"10.1002\/ps.6352","article-title":"An odorant-binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles","volume":"77","author":"Liu","year":"2021","journal-title":"Pest. Manag. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"188","DOI":"10.3389\/fphys.2022.829766","article-title":"Binding affinity characterization of four antennae-enriched odorant-binding proteins from Harmonia axyridis (Coleoptera: Coccinellidae)","volume":"13","author":"Qu","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Yang, L., Tian, X.L., Gui, L.Y., Wang, F.L., and Zhang, G.H. (2023). Key Amino Acid Residues Involved in Binding Interactions between Bactrocera minax Odorant-Binding Protein 3 (BminOBP3) and Undecanol. Insects, 14.","DOI":"10.3390\/insects14090745"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/2\/192\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:50:52Z","timestamp":1760104252000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/2\/192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":58,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["life14020192"],"URL":"https:\/\/doi.org\/10.3390\/life14020192","relation":{},"ISSN":["2075-1729"],"issn-type":[{"type":"electronic","value":"2075-1729"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}