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Innovation Project","award":["101402\/R17088"],"award-info":[{"award-number":["101402\/R17088"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["32273077"],"award-info":[{"award-number":["32273077"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["31472243"],"award-info":[{"award-number":["31472243"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["2022KCXTD014"],"award-info":[{"award-number":["2022KCXTD014"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["040510052201"],"award-info":[{"award-number":["040510052201"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["JCYJ20220530162008018"],"award-info":[{"award-number":["JCYJ20220530162008018"]}]},{"name":"National Natural Science Foundation of ShenZhen","award":["101402\/R17088"],"award-info":[{"award-number":["101402\/R17088"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["32273077"],"award-info":[{"award-number":["32273077"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["31472243"],"award-info":[{"award-number":["31472243"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["2022KCXTD014"],"award-info":[{"award-number":["2022KCXTD014"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["040510052201"],"award-info":[{"award-number":["040510052201"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["JCYJ20220530162008018"],"award-info":[{"award-number":["JCYJ20220530162008018"]}]},{"name":"Program for Scientific Research Start-Fund of Guangdong Ocean University","award":["101402\/R17088"],"award-info":[{"award-number":["101402\/R17088"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Although numerous studies have shown that the hypothalamic\u2013pituitary\u2013adrenal axis plays a vital role in the response to environmental stress by mediating the production of a series of hormones, the mechanism underlying these effects has not been elucidated. This study used proteomics techniques to investigate the differentially expressed proteins (DEPs) in the pituitary glands of pigs and to elucidate the potential changes in the immune\u2013neuroendocrine system under heat stress (HS). In total, 2517 peptides corresponding to 205 proteins were detected. A comparison of the expression patterns between HSs and healthy controls revealed 56 DEPs, of which 31 were upregulated and 25 were downregulated. Ingenuity pathway analysis (IPA) was used to reveal the subcellular characteristics, functional pathways, regulatory networks, and upstream regulators of the identified proteins. The results showed that these differentially expressed proteins were involved in intercellular communication, interactions, apoptosis, nervous system development, functions, abnormalities and other functions, and in the regulatory network. Moreover, the upstream regulators of the differentially expressed proteins were mainly transcriptional regulators, hormones, and cytokines. Thus, the functional network and pathway analyses could provide insights into the complexity and dynamics of HS\u2013host interactions and may accelerate our understanding of the mechanisms underlying HS.<\/jats:p>","DOI":"10.3390\/life14030366","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T06:05:30Z","timestamp":1710137130000},"page":"366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress"],"prefix":"10.3390","volume":"14","author":[{"given":"Qiu","family":"Zhou","sequence":"first","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"},{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"},{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Li","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"},{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Xie","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"},{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4533-5221","authenticated-orcid":false,"given":"Xiaoxi","family":"Liu","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Yong","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youquan","family":"Li","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8910-4089","authenticated-orcid":false,"given":"Zhichao","family":"Yu","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingbin","family":"Ma","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8822-484X","authenticated-orcid":false,"given":"Xianghong","family":"Ju","sequence":"additional","affiliation":[{"name":"Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, China"},{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"ref_1","first-page":"374","article-title":"Effects of Heat Stress on Intestinal Health, Immune System and Meat Quality in Pigs and Its Mechanisms","volume":"29","author":"Xiong","year":"2017","journal-title":"Chin. J. Anim. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1079\/BJN2001356","article-title":"Effect of high temperature on feeding behaviour and heat production in group-housed young pigs","volume":"86","author":"Collin","year":"2001","journal-title":"Br. J. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1071\/AR03249","article-title":"Effects of combined Actinobacillus pleuropneumoniae challenge and change in environmental temperature on calcitonin receptor expression levels in growing pigs","volume":"55","author":"Kerr","year":"2004","journal-title":"Aust. J. Agric. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.5713\/ajas.16.0118","article-title":"Stress, Nutrition, and Intestinal Immune Responses in Pigs\u2014A Review","volume":"29","author":"Lee","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0090-1229(90)90029-P","article-title":"Innate and adaptive immune responses in a social conflict paradigm","volume":"57","author":"Lyte","year":"1990","journal-title":"Clin. Immunol. Immunopathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.4049\/jimmunol.156.7.2608","article-title":"Stress-induced enhancement of antigen-specific cell-mediated immunity","volume":"156","author":"Dhabhar","year":"1996","journal-title":"J. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.bbi.2005.04.006","article-title":"Glucocorticoids produced during exercise may be necessary for optimal virus-induced IL-2 and cell proliferation whereas both catecholamines and glucocorticoids may be required for adequate immune defense to viral infection","volume":"19","author":"Kohut","year":"2005","journal-title":"Brain Behav. Immun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.4049\/jimmunol.162.6.3527","article-title":"Endogenous glucocorticoids protect against cytokine-mediated lethality during viral infection","volume":"162","author":"Ruzek","year":"1999","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"E52","DOI":"10.3168\/jds.S0022-0302(03)74040-5","article-title":"Economic Losses from Heat Stress by US Livestock Industries1","volume":"86","author":"Cobanov","year":"2003","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Fabris, N., and Garaci, E. (1983). Immunoregulation, Plenum Press.","DOI":"10.1007\/978-1-4684-4547-3"},{"key":"ref_11","first-page":"xiii","article-title":"Interleukin 12: Cellular and molecular immunology of an important regulatory cytokine. Introduction","volume":"795","author":"Lotze","year":"1996","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_12","first-page":"79","article-title":"Cellular and molecular immunology of an important regulatory cytokine","volume":"7","author":"Chouaib","year":"1996","journal-title":"Eur. Cytokine Netw."},{"key":"ref_13","first-page":"220","article-title":"Effect of Chaihu Shugan Powder on Rats with Liver-depression Syndrome Induced by Chronic Restrained Stress","volume":"12","author":"Xiong","year":"2004","journal-title":"Chin. J. Integr. Tradit. West. Med. Gastro-Spleen"},{"key":"ref_14","first-page":"496","article-title":"Effect of Hormonal Drugs on Normal SD Rat Hypothalamus-Pituitary-Adrenal Axis Based on H-E Staining and Immunohistochemical Technique","volume":"5","author":"Yu","year":"2015","journal-title":"Pharm. Today"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5397","DOI":"10.1210\/jc.2004-1114","article-title":"Effect of recombinant human growth hormone (GH) replacement on the hypothalamic-pituitary-adrenal axis in adult GH-deficient patients","volume":"89","author":"Claudia","year":"2004","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1530\/eje.0.1440319","article-title":"Age-related changes of the hypothalamic-pituitary-adrenal axis: Pathophysiological correlates","volume":"144","author":"Ferrari","year":"2001","journal-title":"Eur. J. Endocrinol."},{"key":"ref_17","unstructured":"Gual, C. (1969). Progress in Endocrinology: Proceedings of the Third International Congress of Endocrinology, Mexico, June 30\u2013July 5, 1968, Excerpta Medica Foundation."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.2527\/jas.2011-3949","article-title":"Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens: Role of acute hypothalamic-pituitary-adrenal axis activation","volume":"90","author":"Rodrigues","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/j.1600-065X.2008.00669.x","article-title":"Inhibition of interferon induction and signaling by paramyxoviruses","volume":"225","author":"Fontana","year":"2010","journal-title":"Immunol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0065-3527(08)00004-3","article-title":"Chapter 4 Receptor Interactions, Tropism, and Mechanisms Involved in Morbillivirus-Induced Immunomodulation","volume":"71","year":"2008","journal-title":"Adv. Virus Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0022-4804(03)00300-7","article-title":"Glucocorticoid pretreatment induces cytokine overexpression and nuclear factor-\u03baB activation in macrophages","volume":"116","author":"Smyth","year":"2004","journal-title":"J. Surg. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.vetimm.2005.12.001","article-title":"Differential expression analysis of proteins from neutrophils in the periparturient period and neutrophils from dexamethasone-treated dairy cows","volume":"111","author":"Lippolis","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.vetimm.2006.05.004","article-title":"Neutrophil extracellular trap formation by bovine neutrophils is not inhibited by milk","volume":"113","author":"Lippolis","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.3168\/jds.2007-0952","article-title":"Developmental Changes in the Milk Fat Globule Membrane Proteome During the Transition from Colostrum to Milk","volume":"91","author":"Reinhardt","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5785","DOI":"10.1002\/pmic.200600207","article-title":"Identification of proteins of potential diagnostic value for bovine paratuberculosis","volume":"6","author":"Cho","year":"2006","journal-title":"Proteomics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3767","DOI":"10.1002\/pmic.200500730","article-title":"Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development","volume":"6","author":"Connolly","year":"2010","journal-title":"Proteomics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/pmic.200401342","article-title":"Analysis of the Pasteurella multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host","volume":"6","author":"Boyce","year":"2010","journal-title":"Proteomics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6471","DOI":"10.1128\/IAI.72.11.6471-6479.2004","article-title":"Application of Mycobacterial Proteomics to Vaccine Design: Improved Protection by Mycobacterium bovis BCG Prime-Rv3407 DNA Boost Vaccination against Tuberculosis","volume":"72","author":"Mollenkopf","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Zougman","year":"2009","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3831","DOI":"10.1111\/febs.14948","article-title":"Energy conservation in the gut microbe Methanomassiliicoccus luminyensis is based on membrane-bound ferredoxin oxidation coupled to heterodisulfide reduction","volume":"286","author":"Krninger","year":"2019","journal-title":"FEBS J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"11990","DOI":"10.1016\/j.ijhydene.2016.05.101","article-title":"Genome sequence and gene expression studies reveal novel hydrogenases mediated hydrogen production by Clostridium biohydrogenum sp. nov., MCM B-509T","volume":"41","author":"Kamalaskar","year":"2016","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"115111","DOI":"10.1016\/j.envpol.2020.115111","article-title":"Heat stress during late gestation disrupts maternal microbial transmission with altered offspring\u2019s gut microbial colonization and serum metabolites in a pig model","volume":"266","author":"He","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e158","DOI":"10.1038\/emm.2015.5","article-title":"Gephyrin: A central GABAergic synapse organizer","volume":"47","author":"Choii","year":"2015","journal-title":"Exp. Mol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1523\/JNEUROSCI.2533-10.2011","article-title":"Heat Shock Cognate Protein 70 Regulates Gephyrin Clustering","volume":"31","author":"Machado","year":"2011","journal-title":"J. Neurosci. Off. J. Soc. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/bs.mie.2016.11.004","article-title":"Quantitation of Human Metallothionein Isoforms in Cells, Tissues, and Cerebrospinal Fluid by Mass Spectrometry","volume":"586","author":"Shabb","year":"2016","journal-title":"Methods Enzymol."},{"key":"ref_36","first-page":"1457","article-title":"Effect of Metallothionein on Cell Cycle, Apoptosis Rate and Subsets Distribution of Lymphocytes in Peripheral Blood of Dairy Cattle under Heat Stress","volume":"14","author":"Luo","year":"2013","journal-title":"Agric. Ence Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.taap.2013.01.021","article-title":"Zinc protects HepG2 cells against the oxidative damage and DNA damage induced by ochratoxin A","volume":"268","author":"Zheng","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"116735","DOI":"10.1016\/j.lfs.2019.116735","article-title":"Zinc supplementation alleviates OTA-induced oxidative stress and apoptosis in MDCK cells by up-regulating metallothioneins","volume":"234","author":"Li","year":"2019","journal-title":"Life Ences"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0079-6123(06)62013-X","article-title":"Nanoparticles aggravate heat stress induced cognitive deficits, blood-brain barrier disruption, edema formation and brain pathology","volume":"162","author":"Sharma","year":"2007","journal-title":"Prog. Brain Res."},{"key":"ref_40","first-page":"445","article-title":"Histamine modulates heat stress-induced changes in blood-brain barrier permeability, cerebral blood flow, brain oedema and serotonin levels: An experimental study in conscious young rats","volume":"50","author":"Sharma","year":"1992","journal-title":"Neuroence"},{"key":"ref_41","first-page":"81","article-title":"Role of nitric oxide in blood-brain barrier permeability, brain edema and cell damage following hyperthermic brain injury. An experimental study using EGB-761 and Gingkolide B pretreatment in the rat","volume":"76","author":"Sharma","year":"2000","journal-title":"Acta Neurochir. Suppl."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0079-6123(08)62334-1","article-title":"Chapter 27: Age-related pathophysiology of the blood-brain barrier in heat stress","volume":"91","author":"Sharma","year":"1992","journal-title":"Prog. Brain Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1159\/000262529","article-title":"Role of the Growth Hormone\/Insulin-Like Growth Factor 1 Axis in Neurogenesis","volume":"17","author":"Aberg","year":"2010","journal-title":"Endocr. Dev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"843","DOI":"10.3181\/00379727-130-33669","article-title":"Growth Hormone Releasing Activity in the Hypothalamus of Rats Subjected to Prolonged Heat Stress","volume":"130","author":"Parkhie","year":"1969","journal-title":"Exp. Biol. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jtherbio.2018.04.009","article-title":"Effects of chronic thermal stress on growth performance, carcass traits, antioxidant indices and the expression of HSP70, growth hormone and superoxide dismutase genes in two broiler strains","volume":"74","author":"Roushdy","year":"2018","journal-title":"J. Therm. Biol."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/3\/366\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:11:42Z","timestamp":1760105502000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/3\/366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,11]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["life14030366"],"URL":"https:\/\/doi.org\/10.3390\/life14030366","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,11]]}}}