{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T21:51:28Z","timestamp":1787867488060,"version":"build-2784847793"},"reference-count":62,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32172763"],"award-info":[{"award-number":["32172763"]}]},{"name":"National Natural Science Foundation of China","award":["U22A20514"],"award-info":[{"award-number":["U22A20514"]}]},{"name":"National Natural Science Foundation of China","award":["2022YFD130040302"],"award-info":[{"award-number":["2022YFD130040302"]}]},{"name":"National Natural Science Foundation of China","award":["2021BBA083"],"award-info":[{"award-number":["2021BBA083"]}]},{"name":"National Natural Science Foundation of China","award":["202302"],"award-info":[{"award-number":["202302"]}]},{"name":"National Key R&amp;D Program of China","award":["32172763"],"award-info":[{"award-number":["32172763"]}]},{"name":"National Key R&amp;D Program of China","award":["U22A20514"],"award-info":[{"award-number":["U22A20514"]}]},{"name":"National Key R&amp;D Program of China","award":["2022YFD130040302"],"award-info":[{"award-number":["2022YFD130040302"]}]},{"name":"National Key R&amp;D Program of China","award":["2021BBA083"],"award-info":[{"award-number":["2021BBA083"]}]},{"name":"National Key R&amp;D Program of China","award":["202302"],"award-info":[{"award-number":["202302"]}]},{"name":"Hubei Provincial Key R&amp;D Program","award":["32172763"],"award-info":[{"award-number":["32172763"]}]},{"name":"Hubei Provincial Key R&amp;D Program","award":["U22A20514"],"award-info":[{"award-number":["U22A20514"]}]},{"name":"Hubei Provincial Key R&amp;D Program","award":["2022YFD130040302"],"award-info":[{"award-number":["2022YFD130040302"]}]},{"name":"Hubei Provincial Key R&amp;D Program","award":["2021BBA083"],"award-info":[{"award-number":["2021BBA083"]}]},{"name":"Hubei Provincial Key R&amp;D Program","award":["202302"],"award-info":[{"award-number":["202302"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["32172763"],"award-info":[{"award-number":["32172763"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["U22A20514"],"award-info":[{"award-number":["U22A20514"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["2022YFD130040302"],"award-info":[{"award-number":["2022YFD130040302"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["2021BBA083"],"award-info":[{"award-number":["2021BBA083"]}]},{"name":"Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science","award":["202302"],"award-info":[{"award-number":["202302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The aim of this study was to investigate the effects of dietary l-glutamine (Gln) supplementation on the morphology and function of the intestine and the growth of muscle in piglets. In this study, sixteen 21-day-old piglets were randomly divided into two groups: the Control group (fed a basal diet) and the Gln group (fed a basal diet supplemented with 0.81% Gln). Blood, gut, and muscle samples were collected from all piglets on Day 20 of the trial. Compared with the Control group, the supplementation of Gln increased (p &lt; 0.05) the villus height, villus width, villus surface area, and villus height\/crypt depth ratio of the small intestine. Furthermore, the supplementation of Gln increased (p &lt; 0.05) total protein, total protein\/DNA, and RNA\/DNA in both the jejunum and ileum. It also increased (p &lt; 0.05) the concentrations of carnosine and citrulline in the jejunal mucosa, as well as citrulline and cysteine concentrations in the ileum. Conversely, Gln supplementation decreased (p &lt; 0.05) Gln concentrations in both the jejunum and ileum, along with \u03b2-aminoisobutyric acid and 1-Methylhistidine concentrations, specifically in the ileum. Subsequent research revealed that Gln supplementation increased (p &lt; 0.05) the mRNA levels for glutathione-S-transferase omega 2 and interferon-\u03b2 in the duodenum. In addition, Gln supplementation led to an increase (p &lt; 0.05) in the number of Lactobacillus genus in the colon, but a decrease (p &lt; 0.05) in the level of HSP70 in the jejunum and the activity of diamine oxidase in plasma. Also, Gln supplementation reduced (p &lt; 0.05) the mRNA levels of glutathione-S-transferase omega 2 and interferon stimulated genes, such as MX1, OAS1, IFIT1, IFIT2, IFIT3, and IFIT5 in both the jejunum and ileum, and the numbers of Clostridium coccoides, Enterococcus genus, and Enterobacterium family in the colon. Moreover, Gln supplementation enhanced (p &lt; 0.05) the concentrations of total protein, RNA\/DNA, and total protein\/DNA ratio in the longissimus dorsi muscle, the concentrations of citrulline, ornithine, arginine, and hydroxyproline, and the mRNA level of peptide transporter 1, while reducing the contents of hydrogen peroxide and malondialdehyde and the mRNA level of glutathione-S-transferase omega 2 in the longissimus dorsi muscle. In conclusion, dietary Gln supplementation can improve the intestinal function of piglets and promote the growth of the longissimus dorsi muscle.<\/jats:p>","DOI":"10.3390\/life14030405","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T04:36:31Z","timestamp":1710822991000},"page":"405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Dietary l-Glutamine Supplementation on the Intestinal Function and Muscle Growth of Piglets"],"prefix":"10.3390","volume":"14","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100039, China"},{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Shen","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhekun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baocheng","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4852-5124","authenticated-orcid":false,"given":"Shuangshuang","family":"Guo","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed 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