{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T01:52:13Z","timestamp":1782697933764,"version":"3.54.5"},"reference-count":83,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Grant of Excellence Departments 2023\u20132027 (Department of Science, Roma Tre University)","award":["ARTICOLO 1"],"award-info":[{"award-number":["ARTICOLO 1"]}]},{"name":"The Grant of Excellence Departments 2023\u20132027 (Department of Science, Roma Tre University)","award":["COMMI 314\u2013337 LEGGE 232\/2016"],"award-info":[{"award-number":["COMMI 314\u2013337 LEGGE 232\/2016"]}]},{"name":"The Grant of Excellence Departments 2023\u20132027 (Department of Science, Roma Tre University)","award":["M4-C2-Inv. 1.5 CUP F83B22000040006"],"award-info":[{"award-number":["M4-C2-Inv. 1.5 CUP F83B22000040006"]}]},{"name":"Rome Technopole, PNRR","award":["ARTICOLO 1"],"award-info":[{"award-number":["ARTICOLO 1"]}]},{"name":"Rome Technopole, PNRR","award":["COMMI 314\u2013337 LEGGE 232\/2016"],"award-info":[{"award-number":["COMMI 314\u2013337 LEGGE 232\/2016"]}]},{"name":"Rome Technopole, PNRR","award":["M4-C2-Inv. 1.5 CUP F83B22000040006"],"award-info":[{"award-number":["M4-C2-Inv. 1.5 CUP F83B22000040006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Oxidative stress represents a hallmark for many degenerative pathologies of the Central Nervous System. Throughout life, the constant pressure of noxious stimuli and\/or episodes of traumatic events may expose the brain to a microenvironment where the non-balanced reactive oxygen species inevitably lead to neuronal loss and cognitive decline. HO-1, a 32 kDa heat-shock protein catalyzing the degradation of heme into carbon monoxide (CO), iron and biliverdin\/bilirubin is considered one of the main antioxidant defense mechanisms playing pivotal roles in neuroprotection. Restoring the redox homeostasis is the goal of many natural or synthetic antioxidant molecules pursuing beneficial effects on brain functions. Here, we investigated the antioxidant capacity of four selected benzofuran-2-one derivatives in a cellular model of neurodegeneration represented by differentiated SH-SY5Y cells exposed to catechol-induced oxidative stress. Our main results highlight how all the molecules have antioxidant properties, especially compound 9, showing great abilities in reducing intracellular ROS levels and protecting differentiated SH-SY5Y cells from catechol-induced death. This compound above all seems to boost HO-1 mRNA and perinuclear HO-1 protein isoform expression when cells are exposed to the oxidative insult. Our findings open the way to consider benzofuran-2-ones as a novel and promising adjuvant antioxidant strategy for many neurodegenerative disorders.<\/jats:p>","DOI":"10.3390\/life14040422","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T05:10:53Z","timestamp":1711084253000},"page":"422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In Vitro Evaluation of the Antioxidant Capacity of 3,3-Disubstituted-3H-benzofuran-2-one Derivatives in a Cellular Model of Neurodegeneration"],"prefix":"10.3390","volume":"14","author":[{"given":"Sofia","family":"Scibetta","sequence":"first","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7058-672X","authenticated-orcid":false,"given":"Martina","family":"Miceli","sequence":"additional","affiliation":[{"name":"Department of Science, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Iuliano","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0158-5285","authenticated-orcid":false,"given":"Luca","family":"Stefanuto","sequence":"additional","affiliation":[{"name":"Department of Science, University of Roma Tre, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-1073","authenticated-orcid":false,"given":"Elena","family":"Carbone","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Italian National Institute of Health, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0693-4969","authenticated-orcid":false,"given":"Paola","family":"Piscopo","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Italian National Institute of Health, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9837-8690","authenticated-orcid":false,"given":"Vincenzo","family":"Petrozza","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"},{"name":"Istituto Chirurgico Ortopedico Traumatologico (ICOT), 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7638-1001","authenticated-orcid":false,"given":"Giovanna","family":"Romeo","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8950-9780","authenticated-orcid":false,"given":"Giorgio","family":"Mangino","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Calogero","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"},{"name":"Istituto Chirurgico Ortopedico Traumatologico (ICOT), 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3638-2517","authenticated-orcid":false,"given":"Tecla","family":"Gasperi","sequence":"additional","affiliation":[{"name":"Department of Science, University of Roma Tre, 00146 Rome, Italy"},{"name":"National Institute of Biostructures and Biosystems (INBB), 00136 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8468-0677","authenticated-orcid":false,"given":"Paolo","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Medical-Surgical Sciences and Biotechnologies, University of Rome Sapienza, Polo Pontino, 04100 Latina, Italy"},{"name":"Istituto Chirurgico Ortopedico Traumatologico (ICOT), 04100 Latina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.tips.2008.09.001","article-title":"Oxidative stress hypothesis in Alzheimer\u2019s disease: A reappraisal","volume":"29","year":"2008","journal-title":"Trends Pharmacol. 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