{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:11:24Z","timestamp":1789661484615,"version":"4.0.1"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The objective of this study was to evaluate the relationship between the excision type and the persistence\/recurrence of CIN2\u20133. A total of 227 women with CIN2\u20133 who were treated with LLETZ were evaluated. The types of excision according to the IFCPC 2011, volume, cone dimensions, margins of resection, post-cone high-risk human papillomavirus (HR-HPV) status, and viral load were studied. The time to recurrence was assessed using Kaplan\u2013Meier curves. Persistent\/recurrent CIN2\u20133 was found in 12 cases (5.2%). Type 1 excision was performed in 107 patients, with 7 recurrences (6.5%); type 2 excision in 74 patients, with 4 recurrences (5.4%); and type 3 excision in 46 patients, with 1 recurrence (2.1%). The percentage of clear margins in type 1 excisions was 44.9%, that in type 2 excisions was 59.5%, and that in type 3 excisions was 69.6% (p = 0.008). Type 1 excision was associated with 28.5% post-LLETZ HR-HPV positivity, that in type 2 reached 20.6%, and that in type 3 reached 11.4%; this difference was non-significant (p = 0.24). (4) Conclusions: Type 3 excision was associated with a larger proportion of clear margins and lower post-cone HR-HPV positivity, with a lower incidence of the persistence\/recurrence of CIN2\u20133.<\/jats:p>","DOI":"10.3390\/life14080968","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T15:26:53Z","timestamp":1722526013000},"page":"968","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Excision Type, Cone Volume, and Dimensions on Persistence\/Recurrence of Cervical Intraepithelial Neoplasia 2\u20133"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9905-605X","authenticated-orcid":false,"given":"Gonzalo Arturo","family":"Medina Bueno","sequence":"first","affiliation":[{"name":"Medicine and Translational Research Doctorate Program, Faculty of Medicine and Health Sciences, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1792-7545","authenticated-orcid":false,"given":"Maria Eulalia","family":"Fern\u00e1ndez-Montol\u00ed","sequence":"additional","affiliation":[{"name":"Gynecology Department, Hospital Universitari de Bellvitge, IDIBELL, Universitat de Barcelona, L\u2019Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Heydari","sequence":"additional","affiliation":[{"name":"Medicine and Translational Research Doctorate Program, Faculty of Medicine and Health Sciences, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0613-2261","authenticated-orcid":false,"given":"Jordi","family":"Ponce","sequence":"additional","affiliation":[{"name":"Gynecology Department, Hospital Universitari de Bellvitge, IDIBELL, Universitat de Barcelona, L\u2019Hospitalet de Llobregat, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5423-7092","authenticated-orcid":false,"given":"Sara","family":"Tous","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Bellvitge Biomedical Research Institute (IDIBELL), L\u2019Hospitalet de Llobregat, 08908 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Pe\u00f1afiel","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), Bellvitge Biomedical Research Institute (IDIBELL), L\u2019Hospitalet de Llobregat, 08908 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1016\/S1470-2045(17)30700-3","article-title":"Incomplete excision of cervical precancer as a predictor of treatment failure: A systematic review and meta-analysis","volume":"18","author":"Arbyn","year":"2017","journal-title":"Lancet Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100608","DOI":"10.1016\/j.gore.2020.100608","article-title":"Topical therapies for the treatment of cervical intraepithelial neoplasia (CIN) 2\u20133: A narrative review","volume":"33","author":"Desravines","year":"2020","journal-title":"Gynecol. Oncol. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.currproblcancer.2018.01.010","article-title":"Management of cervical premalignant lesions","volume":"42","author":"Basu","year":"2018","journal-title":"Curr. Probl. Cancer"},{"key":"ref_4","unstructured":"NHSCSP (2020, January 30). NHS Cervical Screening Program Colposcopy and Program Management NHSCSP Publication No. 20, 3rd Edition March 2016. NHS Cerv. Screen Program. 3rd Ed.; 2016, Volume 20, pp. 1\u2013108. Available online: https:\/\/www.bsccp.org.uk\/assets\/file\/uploads\/resources\/NHSCSP_20_Colposcopy_and_Programme_Management_(3rd_Edition)_(2).pdf."},{"key":"ref_5","first-page":"1","article-title":"SEGO Oncoguide: Prevention of cervical cancer. Clinical practice guidelines in gynecological and breast cancer","volume":"1","year":"2014","journal-title":"Span. Assoc. Patol. Cerv. Colposc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1097\/AOG.0b013e318254f90c","article-title":"2011 Colposcopic Terminology of the International Federation for Cervical Pathology and Colposcopy","volume":"120","author":"Bornstein","year":"2012","journal-title":"Obstet. Gynecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1001\/jama.287.16.2114","article-title":"The 2001 Bethesda System: Terminology for reporting results of cervical cytology","volume":"287","author":"Solomon","year":"2002","journal-title":"J. Am. Med. Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1097\/LGT.0000000000000283","article-title":"The Accuracy of Large Loop Excision of the Transformation Zone Specimen Dimensions in Determining Volume: A Multicentric Prospective Observational Study","volume":"21","author":"Carcopino","year":"2017","journal-title":"J. Low. Genit. Tract. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"107881","DOI":"10.1016\/j.ypmed.2024.107881","article-title":"Squamocolumnar junction visibility, age, and implications for cervical cancer screening programs","volume":"180","author":"Desai","year":"2024","journal-title":"Prev. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/j.1447-0756.2007.00628.x","article-title":"Cervical intraepithelial neoplasia II-III with endocervical cone margin involvement after cervical loop conization: Is there any predictor for residual disease?","volume":"33","author":"Kietpeerakool","year":"2007","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"172.e1","DOI":"10.1016\/j.ajog.2019.08.042","article-title":"Incomplete excision of cervical intraepithelial neoplasia as a predictor of the risk of recurrent disease\u2014A 16-year follow-up study","volume":"222","author":"Alder","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ejogrb.2011.11.023","article-title":"Follow-up after LLETZ: A study of 682 cases of CIN 2-CIN 3 in a single institution","volume":"161","author":"Lubrano","year":"2012","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.21037\/tcr-22-1466","article-title":"Relationship between positive margin and residual\/recurrence after excision of cervical intraepithelial neoplasia: A systematic review and meta-analysis","volume":"11","author":"Feng","year":"2022","journal-title":"Transl. Cancer Res."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Giannini, A., Di Donato, V., Sopracordevole, F., Ciavattini, A., Ghelardi, A., Vizza, E., and Bogani, G. (2023). Outcomes of High-Grade Cervical Dysplasia with Positive Margins and HPV Persistence after Cervical Conization. Vaccines, 11.","DOI":"10.3390\/vaccines11030698"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1159\/000345864","article-title":"Appropriate cone dimensions to achieve negative excision margins after large loop excision of transformation zone in the uterine cervix for cervical intraepithelial neoplasia","volume":"75","author":"Papoutsis","year":"2013","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1093779","DOI":"10.3802\/jgo.2016.27.e54","article-title":"Identification of appropriate cone length to avoid positive cone margin in high grade cervical intraepithelial neoplasia","volume":"27","author":"Kawano","year":"2016","journal-title":"J. Gynecol. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/aogs.12025","article-title":"The appropriate cone depth to avoid endocervical margin involvement is dependent on age and disease severity","volume":"92","author":"Bae","year":"2023","journal-title":"Acta Obs. Gynecol. Scand."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e385","DOI":"10.1016\/S1470-2045(22)00191-7","article-title":"Terminology for cone dimensions after local conservative treatment for cervical intraepithelial neoplasia and early invasive cervical cancer: 2022 consensus recommendations from ESGO, EFC, IFCPC, and ESP","volume":"23","author":"Kyrgiou","year":"2022","journal-title":"Lancet Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.ygyno.2020.09.034","article-title":"The relationship of human papillomavirus infection with endocervical glandular involvement on cone specimens in women with cervical intraepithelial neoplasia","volume":"159","author":"Spinillo","year":"2020","journal-title":"Gynecol. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Liu, Y., Xu, C., Pan, J., Sun, C., Zhou, H., and Meng, Y. (2021). Significance of the viral load of high-risk HPV in the diagnosis and prediction of cervical lesions: A retrospective study. BMC Womens Health, 21.","DOI":"10.1186\/s12905-021-01493-0"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1097\/PAS.0000000000001818","article-title":"Cervical Precancers Originate from Infected Proliferating Reserve Cells: A Comparative Histologic and Genetic Study of Thin and Thick High-grade Squamous Intraepithelial Lesions","volume":"46","author":"Regauer","year":"2022","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1097\/LGT.0000000000000444","article-title":"Risk Factor Analysis of Persistent High-Grade Squamous Intraepithelial Lesion after Loop Electrosurgical Excision Procedure Conization","volume":"23","author":"Chen","year":"2019","journal-title":"J. Low. Genit. Tract. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Peng, H., Liu, W., Jiang, J., and Du, H. (2023). Extensive lesions and a positive cone margin are strong predictors of residual disease in subsequent hysterectomy following conization for squamous intraepithelial lesion grade 2 or 3 study design. BMC Womens Health, 23.","DOI":"10.1186\/s12905-023-02568-w"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/1471-0528.15996","article-title":"Long-term predictors of residual or recurrent cervical intraepithelial neoplasia 2-3 after treatment with a large loop excision of the transformation zone: A retrospective study","volume":"127","author":"Tous","year":"2020","journal-title":"BJOG Int. J. Obstet. Gynaecol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1002\/ijc.33251","article-title":"Role of human papillomavirus status after conization for high-grade cervical intraepithelial neoplasia","volume":"148","author":"Huang","year":"2021","journal-title":"Int. J. Cancer"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e60","DOI":"10.3802\/jgo.2020.31.e60","article-title":"Age-specific predictors of cervical dysplasia recurrence after primary conization: Analysis of 3212 women","volume":"31","author":"Bogani","year":"2020","journal-title":"J. Gynecol. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1002\/ijgo.13683","article-title":"The treatment strategy of patients with positive margins after cervical cold knife conization-A 7-year retrospective study in China","volume":"156","author":"Fu","year":"2022","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1002\/ijgo.14340","article-title":"Risk factors analysis of recurrent disease after treatment with a loop electrosurgical excision procedure for high-grade cervical intraepithelial neoplasia","volume":"160","author":"Ding","year":"2023","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102171","DOI":"10.1016\/j.jogc.2023.06.006","article-title":"Role of HPV in the Prediction of Persistence\/Recurrence After Treatment for Cervical Precancer","volume":"45","author":"Kulkarni","year":"2023","journal-title":"J. Obs. Gynaecol. Can."},{"key":"ref_30","first-page":"441","article-title":"Risk of recurrent high-grade cervical intraepithelial neoplasia after successful treatment: A long-term multi-cohort study","volume":"12","author":"Kocken","year":"2011","journal-title":"Lacet Oncol."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/8\/968\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:27:15Z","timestamp":1760110035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/8\/968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,31]]},"references-count":30,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["life14080968"],"URL":"https:\/\/doi.org\/10.3390\/life14080968","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,31]]}}}