{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:19:39Z","timestamp":1787620779859,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"East West University Center for Research and Training","award":["EWUCRT (RP)-11(10)-11\/2018"],"award-info":[{"award-number":["EWUCRT (RP)-11(10)-11\/2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Children carrying Staphylococcus aureus in their nasopharynx are at a higher risk of contracting systemic infection. Due to lack of sufficient information regarding such carriage, this study was conducted to explore the prevalence, antibiotic susceptibility, and genomic profiles of S. aureus isolated from nasopharyngeal samples of 163 randomly selected asymptomatic Bangladeshi children aged from 5\u2013&lt;15 years. Antibiotic susceptibility pattern and genomic analysis of the samples were conducted using standard microbiological methods and genomic tools. The carriage was confirmed in 44 (27%) children who were mostly well nourished without respiratory symptoms in the last 3 months. Higher carriage was observed among the younger age group (5\u2013&lt;10 years) who completed vaccines for pneumonia (p = 0.002) and influenza (p = 0.004). Among the isolates, 84.1% were multidrug-resistant and 47.5% (n = 40) were methicillin-resistant S. aureus (MRSA). All the isolates (100%) were resistant to cefixime with higher resistance to ampicillin (95.5%) and penicillin (90.9%). Among the three investigated isolates, two were ST80 (ID-1 and ID-52) and one was a novel strain (ID-19) with the presence of aph-Stph, blaI, blaZ, dha1, fosB, lmrS, mepA, norA, and tet38 genes. The current research demonstrates a high incidence of multidrug-resistant S. aureus and reports the first instance of ST80 in asymptomatic children in Bangladesh.<\/jats:p>","DOI":"10.3390\/life14080971","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T16:14:58Z","timestamp":1722615298000},"page":"971","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antimicrobial Susceptibility and Genomic Profiles of Multidrug-Resistant Staphylococcus aureus from Nasopharynx of Asymptomatic Children in Dhaka, Bangladesh"],"prefix":"10.3390","volume":"14","author":[{"given":"Sufia","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Pharmacy, East West University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishat","family":"Nasrin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, East West University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigar Sultana","family":"Tithi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, East West University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3248-5252","authenticated-orcid":false,"given":"Farjana","family":"Khatun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, East West University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1234-6952","authenticated-orcid":false,"given":"Muhammad","family":"Asaduzzaman","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka 1000, Bangladesh"},{"name":"School of Pharmacy, BRAC University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2965-8108","authenticated-orcid":false,"given":"Anika Fatema","family":"Topa","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, East West University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Farhad","family":"Kabir","sequence":"additional","affiliation":[{"name":"Nutrition & Clinical Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6347-1836","authenticated-orcid":false,"given":"Fahim Kabir Monjurul","family":"Haque","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Natural Sciences, BRAC University, Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0249-0974","authenticated-orcid":false,"given":"Mohammad","family":"Jubair","sequence":"additional","affiliation":[{"name":"Genome Centre, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6876-0191","authenticated-orcid":false,"given":"Mustafizur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Genome Centre, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh"},{"name":"Virology Laboratory, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6419-9168","authenticated-orcid":false,"given":"Christian","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, Pain Management and Perioperative Medicine, Faculty of Medicine, Dalhousie University, Halifax, NS B3H 1X5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e241","DOI":"10.1016\/j.ijid.2008.11.026","article-title":"Prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility pattern in healthcare workers at Namazi Hospital, Shiraz, Iran","volume":"13","author":"Askarian","year":"2009","journal-title":"Int. J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hema-Ouangraoua, S., Tranchot-Diallo, J., Zongo, I., Kabore, N.F., Niki\u00e8ma, F., Yerbanga, R.S., Tinto, H., Chandramohan, D., Ouedraogo, G.A., and Greenwood, B. (2021). Impact of mass administration of azithromycin as a preventive treatment on the prevalence and resistance of nasopharyngeal carriage of Staphylococcus aureus. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0257190"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Esposito, S., Terranova, L., Zampiero, A., Ierardi, V., Rios, W.P., Pelucchi, C., and Principi, N. (2014). Oropharyngeal and nasal Staphylococcus aureus carriage by healthy children. BMC Infect. Dis., 14.","DOI":"10.1186\/s12879-014-0723-9"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e000203","DOI":"10.1136\/bmjresp-2017-000203","article-title":"Burden of bacterial upper respiratory tract pathogens in school children of Nepal","volume":"4","author":"Thapa","year":"2017","journal-title":"BMJ Open Respir. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1093\/trstmh\/tru132","article-title":"Staphylococcus aureus nasopharyngeal carriage in rural and urban northern Vietnam","volume":"108","author":"Zhang","year":"2014","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Guo, Y., Song, G., Sun, M., Wang, J., and Wang, Y. (2020). Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. Front. Cell Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.00107"},{"key":"ref_7","first-page":"188","article-title":"Nasal carriage rate of Staphylococcus aureus and risk factors among healthcare workers and attendants of neonatal intensive care unit in a tertiary care centre in Bangladesh","volume":"6","author":"Moni","year":"2022","journal-title":"J. Pediatr. Perinatol. Child Health"},{"key":"ref_8","first-page":"627","article-title":"Nasal colonization of methicillin resistant Staphylococcus aureus among healthcare providers in a tertiary care hospital, Bangladesh","volume":"28","author":"Taz","year":"2019","journal-title":"Mymensingh Med. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/1469-0691.12705","article-title":"New epidemiology of Staphylococcus aureus infection in Asia","volume":"20","author":"Chen","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00020-18","DOI":"10.1128\/CMR.00020-18","article-title":"Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology","volume":"31","author":"Lakhundi","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1099\/jmm.0.011353-0","article-title":"Characteristics of community- and hospital-acquired meticillin-resistant Staphylococcus aureus strains carrying SCCmec type IV isolated in Malaysia","volume":"58","author":"Ahmad","year":"2009","journal-title":"J. Med. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1089\/mdr.2018.0123","article-title":"Molecular epidemiological characterization of methicillin-susceptible and -resistant Staphylococcus aureus isolated from skin and soft tissue infections in Bangladesh","volume":"25","author":"Haque","year":"2019","journal-title":"Microb. Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mairi, A., Touati, A., and Lavigne, J.P. (2020). Methicillin-resistant Staphylococcus aureus ST80 clone: A systematic review. Toxins, 12.","DOI":"10.3390\/toxins12020119"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sakr, A., Br\u00e9geon, F., M\u00e8ge, J.L., Rolain, J.M., and Blin, O. (2018). Staphylococcus aureus nasal colonization: An update on mechanisms, epidemiology, risk factors, and subsequent infections. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02419"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.tim.2016.06.012","article-title":"Host\u2013bacterial crosstalk determines Staphylococcus aureus nasal colonization","volume":"24","author":"Mulcahy","year":"2016","journal-title":"Trends Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Fabiano, V., Barcellini, L., Sartorio, M.U.A., Pendezza, E., Leone, A., Meneghin, F., Dilillo, D., and Zuccotti, G.V. (2021). Nutritional status of children and adolescents in three Serbian enclaves in Kosovo and Metohija. BMC Public Health, 21.","DOI":"10.1186\/s12889-021-10848-z"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kamruzzaman, M., Rahman, S.A., Akter, S., Shushmita, H., Ali, M.Y., Billah, M.A., Kamal, M.S., Elahi, M.T., and Paul, D.K. (2021). The anthropometric assessment of body composition and nutritional status in children aged 2\u201315 years: A cross-sectional study from three districts in Bangladesh. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0257055"},{"key":"ref_18","first-page":"636","article-title":"Staphylococcus aureus nasal carriers among medical students in a medical school","volume":"67","author":"Nordin","year":"2012","journal-title":"Med. J. Malaysia"},{"key":"ref_19","unstructured":"Katz, S.D., and Coagulase Test Protocol (2019, February 11). American Society for Microbiology. 11 November 2010. Available online: https:\/\/asm.org\/ASM\/media\/Protocol-Images\/Coagulase-Test-Protocol.pdf?ext=.pdf."},{"key":"ref_20","unstructured":"CLSI (2020). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [30th ed.]. CLSI supplement M100."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1093\/jac\/dki372","article-title":"Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA)","volume":"56","author":"Brown","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e01934-17","DOI":"10.1128\/JCM.01934-17","article-title":"CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial Susceptibility Tests","volume":"56","author":"Humphries","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Prjibelski, A., Antipov, D., Meleshko, D., Lapidus, A., and Korobeynikov, A. (2020). Using SPAdes De Novo Assembler. Curr. Protoc. Bioinform., 70.","DOI":"10.1002\/cpbi.102"},{"key":"ref_24","unstructured":"Hussain, Z. (2023, July 25). The Numbers behind Bangladesh\u2019s Goal of Middle Income Status by 2021. World Bank Blogs, Available online: https:\/\/blogs.worldbank.org\/endpovertyinsouthasia\/numbers-behind-bangladesh\u2019s-goal-middle-income-status-2021."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.4103\/jfmpc.jfmpc_288_19","article-title":"Socioeconomic status scales-modified Kuppuswamy and Udai Pareekh\u2019s scale updated for 2019","volume":"8","author":"Wani","year":"2019","journal-title":"J. Fam. Med. Prim. Care"},{"key":"ref_26","first-page":"1000383","article-title":"Nasopharyngeal carriage of Staphylococcus aureus and its antimicrobial resistance pattern among healthy people: Systematic review and meta-analysis","volume":"11","author":"Awulachew","year":"2020","journal-title":"J. Bacteriol. Parasitol."},{"key":"ref_27","first-page":"109","article-title":"Nasopharyngeal carriage rate and antimicrobial susceptibility pattern of potential pathogenic bacteria among paediatrics outpatients at Gondar University Teaching Hospital, Ethiopia","volume":"1","author":"Assefa","year":"2013","journal-title":"J. Infect. Dis. Ther."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0028322","DOI":"10.1128\/iai.00283-22","article-title":"Sex and gender differences in bacterial infections","volume":"90","author":"Dias","year":"2022","journal-title":"Infect. Immun."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Al-Trad, E.I., Chew, C.H., Che Hamzah, A.M., Suhaili, Z., Rahman, N.I.A., Ismail, S., Puah, S.M., Chua, K.H., Kwong, S.M., and Yeo, C.C. (2023). The plasmidomic landscape of clinical methicillin-resistant Staphylococcus aureus isolates from Malaysia. Antibiotics, 12.","DOI":"10.3390\/antibiotics12040733"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1017\/S0950268809991233","article-title":"Staphylococcus aureus nasal carriage rate and associated risk factors in individuals in the community","volume":"138","author":"Halablab","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4103\/jfmpc.jfmpc_345_16","article-title":"Prevalence of nasal colonization of methicillin-resistant Staphylococcus aureus among schoolchildren of Barabanki district, Uttar Pradesh, India","volume":"7","author":"Singh","year":"2018","journal-title":"J. Fam. Med. Prim. Care"},{"key":"ref_32","first-page":"33","article-title":"Resistance pattern of cefixime against uropathogens causing urinary tract infection in selected areas of Dhaka City, Bangladesh","volume":"7","author":"Asaduzzaman","year":"2018","journal-title":"Int. J. Eng. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S2222-1808(14)60312-1","article-title":"Carrier state of Haemophilus influenzae type b (Hib), Streptococcus pneumoniae, Streptococcus pyogenes, Neisseria meningitidis and Corynebacterium diphtheriae among school children in Pokhara, Nepal","volume":"4","author":"Bhatta","year":"2014","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e10230","DOI":"10.1016\/j.heliyon.2022.e10230","article-title":"Evaluation of aminoglycoside modifying enzymes, SCCmec, coagulase gene and PCR-RFLP coagulase gene typing of Staphylococcus aureus isolates from hospitals in Shiraz, southwest of Iran","volume":"8","author":"Fathi","year":"2022","journal-title":"Heliyon"},{"key":"ref_35","first-page":"912","article-title":"Detection of tetracycline resistance genes, aminoglycoside modifying enzymes, and coagulase gene typing of clinical isolates of Staphylococcus aureus in the southwest of Iran","volume":"20","author":"Khoramrooz","year":"2017","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zeng, W., Zhang, X., Liu, Y., Zhang, Y., Xu, M., Wang, S., Sun, Y., Zhou, T., and Chen, L. (2022). In vitro antimicrobial activity and resistance mechanisms of the new generation tetracycline agents, eravacycline, omadacycline, and tigecycline against clinical Staphylococcus aureus isolates. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1043736"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bruce, S.A., Smith, J.T., Mydosh, J.L., Ball, J., Needle, D.B., Gibson, R., and Andam, C.P. (2022). Shared antibiotic resistance and virulence genes in Staphylococcus aureus from diverse animal hosts. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-08230-z"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Mikhaylova, Y., Shelenkov, A., Chernyshkov, A., Tyumentseva, M., Saenko, S., Egorova, A., Manzeniuk, I., and Akimkin, V. (2022). Whole-genome analysis of Staphylococcus aureus isolates from ready-to-eat food in Russia. Foods, 11.","DOI":"10.3390\/foods11172574"},{"key":"ref_39","unstructured":"Amirsoleimani, A. (2020). Investigation into the Presence, Persistence, and Fate of S. aureus, and Its Mobile Genetic Elements, in Wastewater and the Surrounding Environment. [Ph.D. Thesis, University of Kentucky]. Available online: https:\/\/uknowledge.uky.edu\/ce_etds\/104."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhu, Z., Liu, X., Chen, X., Zou, G., Huang, Q., Meng, X., Pei, X., Chen, Z., Zhou, R., and Hu, D. (2022). Prevalence and virulence determinants of Staphylococcus aureus in wholesale and retail pork in Wuhan, Central China. Foods, 11.","DOI":"10.3390\/foods11244114"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/8\/971\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:28:46Z","timestamp":1760110126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/8\/971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,2]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["life14080971"],"URL":"https:\/\/doi.org\/10.3390\/life14080971","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,2]]}}}