{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:47:13Z","timestamp":1782200833922,"version":"3.54.5"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["18\/2021-Faixa"],"award-info":[{"award-number":["18\/2021-Faixa"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["407747\/2021-4"],"award-info":[{"award-number":["407747\/2021-4"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["18\/2021-Faixa"],"award-info":[{"award-number":["18\/2021-Faixa"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["407747\/2021-4"],"award-info":[{"award-number":["407747\/2021-4"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["18\/2021-Faixa"],"award-info":[{"award-number":["18\/2021-Faixa"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["407747\/2021-4"],"award-info":[{"award-number":["407747\/2021-4"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>This study aimed to characterize Pseudomonas aeruginosa strains isolated from hospitalized patients during the COVID-19 pandemic. This was achieved using phenotypic and molecular techniques, including their antimicrobial resistance profile and biofilm formation. Eighteen strains were isolated from a hospital in Rio de Janeiro, Brazil, and identified by VITEK\u00ae2, MALDI-TOF\/MS (VITEK MS\u00ae and MALDI Biotyper\u00ae), and 16S rRNA sequencing. Fourier-transform infrared (FTIR) spectroscopy, antimicrobial susceptibility testing, and biofilm formation and disinfectant tolerance tests were applied to evaluate the virulence characteristics of the strains. VITEK\u00ae2 (\u226599%), VITEK MS\u00ae (\u226582.7%), and MALDI Biotyper\u00ae (score \u2265 2.01) accurately identified the P. aeruginosa strains, but 16S rRNA sequencing did not differentiate the species P. aeruginosa from P. paraeruginosa. FTIR typing identified three different clusters, but no correlation between the phenotypical or antimicrobial susceptibility testing patterns was found. Most strains exhibited resistance to various antimicrobials. The exceptions were sensitivity to amikacin and norfloxacin, and consequently, these could be considered potential treatment options. Most strains (n = 15, 83.3%) produced biofilms on polystyrene. Sodium hypochlorite treatment (0.5%\/15 min) was shown to be the most effective disinfectant for biofilm elimination. P. aeruginosa biofilm formation and tolerance to disinfectants demonstrate the need for effective cleaning protocols to eliminate contamination by this organism in the hospital environment and medical equipment.<\/jats:p>","DOI":"10.3390\/life14091079","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T03:40:39Z","timestamp":1724902839000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy"],"prefix":"10.3390","volume":"14","author":[{"given":"Paula Araujo","family":"de Souza","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology of Food and Sanitizes, INCQS\/Fiocruz, Rio de Janeiro 21040-900, Brazil"},{"name":"Laboratory of Microbiological Control, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena Cristina Silva dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiological Control, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca Vit\u00f3ria da Silva Lage","family":"de Miranda","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiological Control, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1873-4056","authenticated-orcid":false,"given":"Luciana Veloso","family":"da Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiological Control, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael Paiva Paschoal","family":"da Silva","sequence":"additional","affiliation":[{"name":"Hospital de For\u00e7a A\u00e9rea do Gale\u00e3o, For\u00e7a \u00c1rea Brasileira, Rio de Janeiro 21941-353, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8917-1249","authenticated-orcid":false,"given":"Catia Aparecida Chaia","family":"de Miranda","sequence":"additional","affiliation":[{"name":"Interdisciplinary Medical Research Laboratory, IOC\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9076-0091","authenticated-orcid":false,"given":"Ana Paula Roque","family":"da Silva","sequence":"additional","affiliation":[{"name":"Analytical Indicators and Data Systems Section, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6709-0712","authenticated-orcid":false,"given":"Stephen James","family":"Forsythe","sequence":"additional","affiliation":[{"name":"Foodmicrobe.com Ltd., Adams Hill, Keyworth, Nottingham NG12 5GY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Helena Sim\u00f5es Villas","family":"B\u00f4as","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology of Food and Sanitizes, INCQS\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-7312","authenticated-orcid":false,"given":"Marcelo Luiz Lima","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiological Control, Bio-Manguinhos\/Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1099\/jmm.0.001500","article-title":"Localized outbreaks of Pseudomonas aeruginosa belonging to international high-risk clones in a south Indian hospital","volume":"71","author":"Pulusu","year":"2022","journal-title":"J. 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