{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:25:10Z","timestamp":1784863510043,"version":"3.55.0"},"reference-count":76,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICUB Young Research Grant","award":["7412\/04.07.2023"],"award-info":[{"award-number":["7412\/04.07.2023"]}]},{"name":"ICUB Young Research Grant","award":["7423\/04.07.2023"],"award-info":[{"award-number":["7423\/04.07.2023"]}]},{"name":"ICUB Young Research Grant","award":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"]}]},{"name":"Research Institute of the University of Bucharest","award":["7412\/04.07.2023"],"award-info":[{"award-number":["7412\/04.07.2023"]}]},{"name":"Research Institute of the University of Bucharest","award":["7423\/04.07.2023"],"award-info":[{"award-number":["7423\/04.07.2023"]}]},{"name":"Research Institute of the University of Bucharest","award":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"]}]},{"DOI":"10.13039\/501100006595","name":"UEFISCDI","doi-asserted-by":"publisher","award":["7412\/04.07.2023"],"award-info":[{"award-number":["7412\/04.07.2023"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"UEFISCDI","doi-asserted-by":"publisher","award":["7423\/04.07.2023"],"award-info":[{"award-number":["7423\/04.07.2023"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"UEFISCDI","doi-asserted-by":"publisher","award":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2021-2526 (736 PED\/2022)"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Epiphytic yeasts represent an important source for the development of novel strategies aiming to combat food microbial contamination. The present study deals with the characterization of nine yeast strains belonging to Starmerella, Candida, Metschinikowia, Lachancea, Kodamaea and Pichia genera, isolated from the surface of plants from the Botanical Garden \u201cDimitrie Brandza\u201d (Bucharest, Romania) for use as antimicrobial and probiotic agents. The tests involved the determination of the safe status, cell growth under stress conditions, and activity against pathogenic Candida and bacteria strains, respectively, as well as phytopathogenic filamentous fungi and lipolytic activity. None of the nine strains showed all the characteristics for virulence and pathogenicity, with the rare positive results being explained rather by their adaptability to the habitats of origin. The strains Lachancea thermotolerans CMGB-ST12 and Kodamaea ohmeri CMGB-ST19 grew at 37 \u00b0C; Metschnikowia reukaufii CMGB-ST21.2, M. reukaufii CMGB-ST.8.1 and M. reukaufii CMGB ST10 grew in the presence of 10% NaCl, while L. thermotolerans CMGB-ST12 and K. ohmeri CMGB-ST19 tolerated both acidic and alkaline pH values well (3.0 to 12.0). The studied yeast strains showed good antimicrobial activity against Candida krusei, Candida albicans and Gram-negative bacterial strains, with K. ohmeri CMGB-ST19 and Pichia membranaefaciens CMGB-ST53 inhibiting up to 100% the development of filamentous fungi. All the strains produced lipases for tributyrin hydrolysis, the best producer being Starmerella bombi CMGB-ST1, and only Candida magnoliae CMGB-ST8.2 tested positive against other probiotic yeasts. Overall, our nine yeast strains show high potential for industrial applications, for obtaining probiotic products and for preventing the development of a wide range of microbial food contaminants.<\/jats:p>","DOI":"10.3390\/life14091087","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T11:12:13Z","timestamp":1724929933000},"page":"1087","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Epiphytic Yeasts from South Romania for Preventing Food Microbial Contamination"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5334-0111","authenticated-orcid":false,"given":"Viorica Maria","family":"Corbu","sequence":"first","affiliation":[{"name":"Department of Genetics, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, 060101 Bucharest, Romania"},{"name":"Research Institute of University of Bucharest (ICUB), University of Bucharest, B.P. Hasdeu Street 7, 050568 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreea \u0218tefania","family":"Dumbrav\u0103","sequence":"additional","affiliation":[{"name":"Department of Technological Irradiation (IRASM), Horia Hulubei National Institute of Physics and Nuclear Engineering\u2014IFIN-HH, 077125 M\u0103gurele, Romania"},{"name":"Department of Microbiology and Botany, Faculty of Biology, University of Bucharest, Aleea Portocalelor, no. 1\u20133, 060101 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Gheorghe-Barbu","sequence":"additional","affiliation":[{"name":"Research Institute of University of Bucharest (ICUB), University of Bucharest, B.P. Hasdeu Street 7, 050568 Bucharest, Romania"},{"name":"Department of Microbiology and Botany, Faculty of Biology, University of Bucharest, Aleea Portocalelor, no. 1\u20133, 060101 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ortansa","family":"Csutak","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, 060101 Bucharest, Romania"},{"name":"Research Institute of University of Bucharest (ICUB), University of Bucharest, B.P. 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