{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:46:13Z","timestamp":1764701173180,"version":"build-2065373602"},"reference-count":75,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>(1) Background: Periocular or periorbital dermatitis is a common term for all inflammatory skin diseases affecting the area of skin around the eyes. The clear etiopathogenesis of periocular dermatitis is still not fully understood. Advances in molecular techniques for studying microorganisms living in and on our bodies have highlighted the microbiome as a possible contributor to disease, as well as a promising diagnostic marker and target for innovative treatments. The aim of this study was to compare the composition and diversity of the skin microbiota in the periocular region between healthy individuals and individuals affected by the specific entity of periocular dermatitis. (2) Methods: A total of 35 patients with periocular dermatitis and 39 healthy controls were enrolled in the study. After a skin swab from the periocular region was taken from all participants, DNA extraction and 16S rRNA gene amplicon sequencing using Illumina NovaSeq technology were performed. (3) Results: Staphylococcus and Corynebacterium were the most abundant bacterial genera in the microbiota of healthy skin. Analysis of alpha diversity revealed a statistically significant change (p &lt; 0.05) in biodiversity based on the Faith\u2019s PD index between patients and healthy individuals. We did not observe changes in beta diversity. The linear discriminant analysis effect size (LEfSe) revealed that Rothia, Corynebacterium, Bartonella, and Paracoccus were enriched in patients, and Anaerococcus, Bacteroides, Porphyromonas, and Enhydrobacter were enriched in healthy controls. (4) Conclusions: According to the results obtained, we assume that the observed changes in the bacterial microbiota on the skin, particularly Gram-positive anaerobic cocci and skin commensals of the genus Corynebacterium, could be one of the factors in the pathogenesis of the investigated inflammatory diseases. The identified differences in the microbiota between healthy individuals and patients with periocular dermatitis should be further investigated.<\/jats:p>","DOI":"10.3390\/life14091091","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T06:46:29Z","timestamp":1725000389000},"page":"1091","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of the Skin Microbiota in the Periocular Region between Patients with Inflammatory Skin Diseases and Healthy Participants: A Preliminary Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Iva","family":"Fer\u010dek","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Zabok General Hospital and Croatian Veterans\u2019 Hospital, 49210 Zabok, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9757-6636","authenticated-orcid":false,"given":"Petar","family":"Ozreti\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Hereditary Cancer, Division of Molecular Medicine, Ru\u0111er Bo\u0161kovi\u0107 Institute, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-1465","authenticated-orcid":false,"given":"Arjana","family":"Tambi\u0107-Andra\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Clinical Microbiology, University Hospital for Infectious Diseases, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6667-8955","authenticated-orcid":false,"given":"Slave","family":"Trajanoski","sequence":"additional","affiliation":[{"name":"Core Facility Computational Bioanalytics, Center for Medical Research, Medical University Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"\u0106esi\u0107","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Medikol Clinic, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3554-3668","authenticated-orcid":false,"given":"Marko","family":"Jeli\u0107","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, University Hospital for Infectious Diseases, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goran","family":"Geber","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Otorhinolaryngology and Head and Neck Surgery, Sestre Milosrdnice University Hospital Centre, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3987-8008","authenticated-orcid":false,"given":"Orjena","family":"\u017daja","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Pediatrics, Sestre Milosrdnice University Hospital Centre, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josipa","family":"Pai\u0107","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Optometry, \u0160ibenik General Hospital, 22000 \u0160ibenik, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-5742","authenticated-orcid":false,"given":"Liborija","family":"Lugovi\u0107-Mihi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Dermatology and Venereology, Sestre Milosrdnice University Hospital Centre, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8766-7438","authenticated-orcid":false,"given":"Rok","family":"\u010civljak","sequence":"additional","affiliation":[{"name":"Department for Respiratory Infections, University Hospital for Infectious Diseases \u201cDr. Fran Mihaljevi\u0107\u201d, 10000 Zagreb, Croatia"},{"name":"School of Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.clindermatol.2013.05.035","article-title":"Periorbital (eyelid) dermatides","volume":"32","author":"Wolf","year":"2014","journal-title":"Clin. 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