{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:53:00Z","timestamp":1787028780203,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>This study evaluates the utilization of in vitro embryo production (IVEP) technology for the conservation and breeding of the Auliekol cattle breed, a primary beef breed in Kazakhstan facing population decline due to the cessation of breeding programs and the incursion of transboundary diseases. We assessed the effect of consecutive ovum pick-up (OPU) procedures on oocyte yield and embryo production in Auliekol and Aberdeen Angus cows. A total of 2232 and 3659 oocytes were aspirated from Auliekol and Aberdeen Angus donors, respectively, with significantly higher yields and embryo production observed in Aberdeen Angus cows. The application of a meiotic block using Butyrolactone I (BLI) and subsequent in vitro fertilization (IVF) protocols was employed, with embryo development monitored up to the morula\/blastocyst stage. Results indicated that Auliekol cows exhibited lower oocyte recovery, cleavage, and blastocyst rates compared to Aberdeen Angus cows, likely due to genetic characteristics. Despite the challenges, IVEP presents a valuable tool for the preservation and future propagation of the Auliekol breed, highlighting the need for further research to enhance reproductive outcomes and conservation strategies.<\/jats:p>","DOI":"10.3390\/life14091167","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:14:07Z","timestamp":1726460047000},"page":"1167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Utilization of Assisted Reproductive Technologies in Breeding Auliekol Cattle: A Comparative Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Altyn","family":"Kulpiisova","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairly","family":"Yessengaliyev","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulsara","family":"Kassimova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainat","family":"Kozhakhmetova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bakytkanym","family":"Kadraliyeva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeldinov","family":"Rustem","sequence":"additional","affiliation":[{"name":"Department of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma","family":"Temirzhanova","sequence":"additional","affiliation":[{"name":"Department of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Burambayeva","sequence":"additional","affiliation":[{"name":"Department of Zootechnology, Genetics and Breeding, Toraighyrov University, Pavlodar 140000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salbak","family":"Chylbak-ool","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow 127343, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Pakhomova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow 127343, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurzhan","family":"Abekeshev","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Zhangir Khan West Kazakhstan Agrarian Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulnara","family":"Baikadamova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhomart","family":"Kemeshev","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Tegza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0486-0054","authenticated-orcid":false,"given":"Arman","family":"Issimov","sequence":"additional","affiliation":[{"name":"Department of Biology, K.Zhubanov Aktobe Regional University, Aktobe 030000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9742-4939","authenticated-orcid":false,"given":"Peter","family":"White","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1093\/biolre\/ioz025","article-title":"Assisted reproductive technologies for endangered species conservation: Developing sophisticated protocols with limited access to animals with unique reproductive mechanisms","volume":"100","author":"Herrick","year":"2019","journal-title":"Biol. 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Agriculture, 12.","DOI":"10.3390\/agriculture12091357"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/9\/1167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:56:55Z","timestamp":1760111815000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/9\/1167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,15]]},"references-count":42,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["life14091167"],"URL":"https:\/\/doi.org\/10.3390\/life14091167","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,15]]}}}