{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T02:24:40Z","timestamp":1778811880326,"version":"3.51.4"},"reference-count":69,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Given progressive population ageing and the increase in the number of patients with comorbidities, the management of chronic and\/or hard-to-heal wounds (HHWs) nowadays represents a common problem in many clinical settings. In these cases, standard strategies may not be sufficient. Autologous grafting represent the gold standard for permanent wound closure, but is almost never realized when the skin loss is extensive\/the patient is young. The grafting of homologous skin\/dermal tissue procured from cadaver donors (i.e., allografting) represents the best alternative, especially when the dermal component is lost. This request supports the activities of skin bank establishments (including donor screening, skin procurement, processing, storage, and distribution) that are regulated by specific guidelines and need to continuously meet quality standard requirements. The aim of this work is to both give specific insights of all the procedures implied in allograft preparation as well as an overview of their practical application in the treatment of different HHWs. The particular characteristics of each skin\/dermal allograft released by Siena Skin Bank (cryopreserved\/glycerol-preserved skin\/de-epidermized dermis, acellular lyophilized de-epidermized dermis\/reticular dermis) are also discussed. The exemplificative series of HHWs managed in the Dermatology Department of Siena were classified according their etiology into post-traumatic, vascular (arterial\/venous\/mixed\/lymphatic), inflammatory, surgical, and heat\/chemical burns. Globally, the clinical advantages obtained include: acceleration of healing process, pain sparing, resistance to bacterial contamination, dermal regeneration (instead of scarring), and better aesthetic\u2013functional outcome.<\/jats:p>","DOI":"10.3390\/life14101285","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T08:10:16Z","timestamp":1728634216000},"page":"1285","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["From Bank Preparation to Clinical Use of Homologous Skin Allografts in Wound Healing: A Sustainable Approach"],"prefix":"10.3390","volume":"14","author":[{"given":"Laura","family":"Amoroso","sequence":"first","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serena","family":"Agueci","sequence":"additional","affiliation":[{"name":"Dermatology Unit, Department of Medical, Surgical and Neurosciences, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Pianigiani","sequence":"additional","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"},{"name":"Dermatology Unit, Department of Medical, Surgical and Neurosciences, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Ierardi","sequence":"additional","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"},{"name":"Dermatology Unit, Department of Medical, Surgical and Neurosciences, University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5238-2336","authenticated-orcid":false,"given":"Laura","family":"Calabrese","sequence":"additional","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Rubegni","sequence":"additional","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6691-4310","authenticated-orcid":false,"given":"Linda","family":"Tognetti","sequence":"additional","affiliation":[{"name":"Skin Bank Unit, Azienda Ospedaliera Universitaria Senese (AOUS), 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1159\/000454919","article-title":"Skin Wound Healing: An Update on the Current Knowledge and Concepts","volume":"58","author":"Sorg","year":"2017","journal-title":"Eur. Surg. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1159\/000505291","article-title":"Optimal Support of Wound Healing: New Insights","volume":"236","author":"Baron","year":"2020","journal-title":"Dermatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s12325-017-0478-y","article-title":"Chronic Wound Healing: A Review of Current Management and Treatments","volume":"34","author":"Han","year":"2017","journal-title":"Adv. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/wrr.12384","article-title":"Rapid Identification of Slow Healing Wounds","volume":"24","author":"Jung","year":"2016","journal-title":"Wound Repair. Regen."},{"key":"ref_5","unstructured":"Elseth, A., and Nunez Lopez, O. (2024, April 01). Wound Grafts, StatPearls, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK564382\/."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.clindermatol.2004.07.018","article-title":"Guidelines on Processing and Clinical Use of Skin Allografts","volume":"23","author":"Kearney","year":"2005","journal-title":"Clin. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Vecin, N.M., and Kirsner, R.S. (2023). Skin Substitutes as Treatment for Chronic Wounds: Current and Future Directions. Front. Med., 10.","DOI":"10.3389\/fmed.2023.1154567"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.clindermatol.2004.07.016","article-title":"Skin Bank Organization","volume":"23","author":"Pianigiani","year":"2005","journal-title":"Clin. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s10561-018-9715-0","article-title":"Cadaver Skin Allograft May Improve Mortality Rate for Burns Involving over 30% of Total Body Surface Area: A Propensity Score Analysis of Data from Four Burn Centers","volume":"19","author":"Choi","year":"2018","journal-title":"Cell Tissue Bank."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.clindermatol.2004.07.021","article-title":"Performance and safety of skin allografts","volume":"23","year":"2005","journal-title":"Clin. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e14374","DOI":"10.1111\/iwj.14374","article-title":"Organization of Hannover Skin Bank: Sterile Culture and Procurement Protocols for Viable Cryopreserved Allogeneic Skin Grafts of Living Donors","volume":"21","author":"Schlottmann","year":"2024","journal-title":"Int. Wound J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.burns.2005.10.005","article-title":"Prevalence of Skin Allograft Discards as a Result of Serological and Molecular Microbiological Screening in a Regional Skin Bank in Italy","volume":"32","author":"Pianigiani","year":"2006","journal-title":"Burns"},{"key":"ref_13","unstructured":"European Commission (2024, June 10). EudraLex\u2014Volume 4\u2014Good Manufacturing Practice (GMP) Guidelines. Available online: https:\/\/ec.europa.eu\/health\/documents\/eudralex\/vol-4_en."},{"key":"ref_14","unstructured":"European Directorate for the Quality of Medicines & Health Care (EDQM) (2022). Guide to the Quality and Safety of Tissues and Cells for Human Application, Council of Europe. [5th ed.]."},{"key":"ref_15","unstructured":"(2024, March 01). Linee Guida per il Prelievo, la Processazione e la Distribuzione dei Tessuti a Scopo di Trapianto. Centro Nazionale per i Trapianti (CNT). Linee guida Centro Nazionale Tra- pianti 14 settembre 2016, LG.Tessuti, Available online: https:\/\/www.trapianti.salute.gov.it\/trapianti\/homeCnt.jsp."},{"key":"ref_16","unstructured":"(2015). Cleanrooms and Associated Controlled Environments\u2014Part 1: Classification of Air Cleanliness by Particle Concentration. ISO Technical Committee 209 (ISO\/TC 209) (Standard No. EN ISO 14644-1: 2015)."},{"key":"ref_17","unstructured":"(2001). Cleanrooms and Associated Controlled Environments\u2014Part 4: Design, Construction and Start-Up. ISO Technical Committee 209 (ISO\/TC 209) (Standard No. EN ISO 14644-4: 2001)."},{"key":"#cr-split#-ref_18.1","unstructured":"(2015). Cleanrooms and Associated Controlled Environments-Part 2: Monitoring to Provide Evidence of Cleanroom Performance Related to Air Cleanliness by Particle Concentration"},{"key":"#cr-split#-ref_18.2","unstructured":"ISO Technical Committee 209 (ISO\/TC 209) (Standard No. EN ISO 14644-2: 2015)."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s10561-022-10033-5","article-title":"Skin Donations after Suicide by Acute Poisoning","volume":"24","author":"Amoroso","year":"2023","journal-title":"Cell Tissue Bank."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s10561-016-9550-0","article-title":"Assessment of Cryopreserved Donor Skin Viability: The Experience of the Regional Tissue Bank of Siena","volume":"17","author":"Pianigiani","year":"2016","journal-title":"Cell Tissue Bank."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.burns.2013.09.031","article-title":"Cryopreserved Human Skin Allografts: Efficacy and Viability","volume":"40","author":"Gaucher","year":"2014","journal-title":"Burns"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2147\/BSAM.S115187","article-title":"Current Insights into Skin Banking: Storage, Preservation and Clinical Importance of Skin Allografts","volume":"5","author":"Tognetti","year":"2017","journal-title":"BSAM"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.burns.2013.05.006","article-title":"Clinical Application and Viability of Cryopreserved Cadaveric Skin Allografts in Severe Burn: A Retrospective Analysis","volume":"40","author":"Cleland","year":"2014","journal-title":"Burns"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0305-4179(99)00169-2","article-title":"Effect of Storage and Preservation Methods on Viability in Transplantable Human Skin Allografts","volume":"26","author":"Bravo","year":"2000","journal-title":"Burns"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.burns.2003.01.001","article-title":"Evaluation of Donor Skin Viability: Fresh and Cryopreserved Skin Using Tetrazolioum Salt Assay","volume":"29","author":"Castagnoli","year":"2003","journal-title":"Burns"},{"key":"ref_26","first-page":"100","article-title":"Evaluation of Cryopreserved Donor Skin Viability: The Experience of the Regional Tissue Bank of Verona","volume":"7","author":"Franchini","year":"2009","journal-title":"Blood Transfus."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/0970-0358.53017","article-title":"The Versatility of a Glycerol-Preserved Skin Allograft as an Adjunctive Treatment to Free Flap Reconstruction","volume":"42","author":"Khoo","year":"2009","journal-title":"Indian. J. Plast. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.burns.2011.01.007","article-title":"Preservation Methods of Allografts and Their (Lack of) Influence on Clinical Results in Partial Thickness Burns","volume":"37","author":"Hermans","year":"2011","journal-title":"Burns"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mosti, G., Mattaliano, V., Picerni, P., and Christou, C. (2018). Skin Grafting in the Treatment of Hard-to-Heal Leg Ulcers. Ital. J. Wound Care, 2.","DOI":"10.4081\/ijwc.2018.18"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1001\/archotol.124.11.1201","article-title":"The Use of Processed Allograft Dermal Matrix for Intraoral Resurfacing: An Alternative to Split-Thickness Skin Grafts","volume":"124","author":"Rhee","year":"1998","journal-title":"Arch. Otolaryngol. Head. Neck Surg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e284","DOI":"10.1097\/GOX.0000000000000219","article-title":"Dermal Matrices and Bioengineered Skin Substitutes: A Critical Review of Current Options","volume":"3","author":"Debels","year":"2015","journal-title":"Plast. Reconstr. Surg. Glob. Open"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s00192-008-0717-5","article-title":"Review of Synthetic Mesh-Related Complications in Pelvic Floor Reconstructive Surgery","volume":"20","author":"Bako","year":"2009","journal-title":"Int. Urogynecol J. Pelvic Floor. Dysfunct."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1097\/PRS.0b013e31820a6511","article-title":"The Use of Acellular Dermal Matrix in Release of Burn Contracture Scars in the Hand","volume":"127","author":"Askari","year":"2011","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_34","unstructured":"(2022, July 25). EN 17141: 2020; Cleanrooms and Associated Controlled Environments\u2014Biocontamination Control. Available online: https:\/\/standards.iteh.ai\/catalog\/standards\/cen\/786bdc13-6821-4f3d-800a-96e24631df62\/en-17141-2020."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0733-8635(05)70295-3","article-title":"Use of skin substitutes in dermatology","volume":"19","author":"Falabella","year":"2001","journal-title":"Dermatol. Clin."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"20595131211038313","DOI":"10.1177\/20595131211038313","article-title":"Clinical applications of acellular dermal matrices: A review","volume":"8","author":"Petrie","year":"2022","journal-title":"Scars Burn. Heal."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/978-3-030-45351-0_41","article-title":"Skin Bank Bioproducts: The Basics","volume":"41","author":"Tognetti","year":"2020","journal-title":"Technol. Pract. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/978-3-030-45351-0_42","article-title":"Clinical applications of skin bank bioproducts","volume":"42","author":"Tognetti","year":"2020","journal-title":"Technol. Pract. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e14987","DOI":"10.1111\/dth.14987","article-title":"The use of human acellular dermal matrices in advanced wound healing and surgical procedures: State of the art","volume":"34","author":"Tognetti","year":"2021","journal-title":"Dermatol. Ther."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1097\/DSS.0000000000001990","article-title":"Cadaveric Skin Grafts May Greatly Increase the Healing Rate of Recalcitrant Ulcers When Used Both Alone and in Combination with Split-Thickness Skin Grafts","volume":"46","author":"Mosti","year":"2020","journal-title":"Dermatol. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/iwj.12575","article-title":"Skin grafting for the treatment of chronic leg ulcers\u2013a systematic review in evidence-based medicine","volume":"14","author":"Serra","year":"2017","journal-title":"Int. Wound J."},{"key":"ref_42","first-page":"13","article-title":"Assessment of biological response of lyophilized cadaveric skin allograft in post burn raw area and nonhealing ulcers","volume":"24","author":"Agarwal","year":"2016","journal-title":"Indian J. Burn."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103233","DOI":"10.1016\/j.amjoto.2021.103233","article-title":"Biologics and acellular dermal matrices in head and neck reconstruction: A comprehensive review","volume":"43","author":"Patel","year":"2022","journal-title":"Am. J. Otolaryngol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"S51","DOI":"10.33549\/physiolres.935045","article-title":"Acellular Dermal Matrix in Plastic and Reconstructive Surgery","volume":"71","author":"Koller","year":"2022","journal-title":"Physiol. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.sxmr.2020.07.003","article-title":"Acellular Dermal Matrix Tissues in Genitourinary Reconstructive Surgery: A Review of the Literature and Case Discussions","volume":"9","author":"Haney","year":"2021","journal-title":"Sex. Med. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e2024076","DOI":"10.5826\/dpc.1401a76","article-title":"Successful Treatment of Pyoderma Gangrenosum with Composite Grafting of Acellular Dermal Matrix and Glycerolized Skin: A Preliminary Experience","volume":"14","author":"Tognetti","year":"2024","journal-title":"Dermatol. Pract. Concept."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.cll.2005.06.008","article-title":"Human skin banking","volume":"25","author":"Kagan","year":"2005","journal-title":"Clin. Lab. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Kagan, R.J., Winter, R., and Robb, E.C. (2012). The skin bank. Total Burn Care, Saunders Elsevier. [4th ed.].","DOI":"10.1016\/B978-1-4377-2786-9.00015-1"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/0305-4179(94)90080-9","article-title":"Euro Skin Bank: Large scale skin-banking in Europe based on glycerol-preservation of donor skin","volume":"20","year":"1994","journal-title":"Burns"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.burns.2020.07.004","article-title":"How to improve donor skin availability: Pragmatic procedures to minimize the discard rate of cryopreserved allografts in skin banking","volume":"47","author":"Germain","year":"2021","journal-title":"Burns"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"S114","DOI":"10.4103\/0970-0358.70732","article-title":"Deceased donor skin allograft banking: Response and utilization","volume":"43","author":"Gore","year":"2010","journal-title":"Indian J. Plast. Surg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10561-009-9152-1","article-title":"The use of human deceased donor skin allograft in burn care","volume":"11","author":"Eldardiri","year":"2010","journal-title":"Cell Tissue Bank."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.burns.2008.04.001","article-title":"Microbiological assessment of cadaver skin grafts received in a Skin Bank","volume":"35","author":"Mathur","year":"2009","journal-title":"Burns"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1097\/00007890-199804150-00017","article-title":"Microbiological evaluation of glycerolized cadaveric donor skin","volume":"65","author":"Ligtvoet","year":"1998","journal-title":"Transplantation"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"327","DOI":"10.23873\/2074-0506-2018-10-4-327-335","article-title":"Use of cadaver skin in the treatment of wounds","volume":"10","author":"Sachkov","year":"2018","journal-title":"Transplantologi\u00e2"},{"key":"ref_56","first-page":"914","article-title":"Skin For Life: Skin Donation And Skin Banking","volume":"30","author":"Verma","year":"2024","journal-title":"Educ. Adm. Theory Pract."},{"key":"ref_57","first-page":"487","article-title":"Understanding and Perceptions of Skin Donation and Skin Banking among Health Care Professionals\u2013Tertiary Care Centre in Eastern India","volume":"4","author":"Arunachalam","year":"2021","journal-title":"Int. J. Cur. Sci. Res. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1016\/j.burns.2014.03.009","article-title":"Awareness and attitude of doctors and nurses at a teaching hospital to skin donation and banking","volume":"40","author":"Michael","year":"2014","journal-title":"Burns"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4103\/0970-0358.129652","article-title":"Knowledge and awareness of skin donation in medical students: Overcoming the barriers","volume":"47","author":"Panse","year":"2014","journal-title":"Indian J. Plast. Surg."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/wrr.12551","article-title":"A randomized clinical trial of a human acellular dermal matrix demonstrated superior healing rates for chronic diabetic foot ulcers over conventional care and an active acellular dermal matrix comparator","volume":"25","author":"Cazzell","year":"2017","journal-title":"Wound Repair Regen"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/iwj.12600","article-title":"A prospective, randomised, controlled, multicentre clinical trial examining healing rates, safety and cost to closure of an acellular reticular allogenic human dermis versus standard of care in the treatment of chronic diabetic foot ulcers","volume":"14","author":"Zelen","year":"2017","journal-title":"Int. Wound J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1080\/09546634.2018.1530443","article-title":"Skin substitutes for acute and chronic wound healing: An updated review","volume":"31","author":"Dai","year":"2020","journal-title":"J. Dermatol. Treat."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"tkz003","DOI":"10.1093\/burnst\/tkz003","article-title":"Strategies to prevent hypertrophic scar formation: A review of therapeutic interventions based on molecular evidence","volume":"8","author":"Shirakami","year":"2020","journal-title":"Burn. Trauma"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1093\/jbcr\/iraa003","article-title":"The optimal treatment for partial thickness burns: A cost-utility analysis of skin allograft vs. topical silver dressings","volume":"41","author":"Sheckter","year":"2020","journal-title":"J. Burn Care Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.burnso.2017.07.002","article-title":"Effects of demographic and socioeconomic factors on the use of skin substitutes in burn patients","volume":"1","author":"Nygaard","year":"2017","journal-title":"Burn. Open"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131575","DOI":"10.1016\/j.colsurfa.2023.131575","article-title":"Evaluation of biogenic nanosilver-acticoat for wound healing: A tri-modal in silico, in vitro and in vivo study","volume":"670","author":"Singh","year":"2023","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Singh, A.V., Gemmati, D., Kanase, A., Pandey, I., Misra, V., Kishore, V., Jahnke, T., and Bill, J. (2018). Nanobiomaterials for vascular biology and wound management: A review. Veins Lymphat., 7.","DOI":"10.4081\/vl.2018.7196"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Akita, S., Hayashida, K., Yoshimoto, H., Fujioka, M., Senju, C., Morooka, S., Nishimura, G., Mukae, N., Kobayashi, K., and Anraku, K. (2017). Novel Application of Cultured Epithelial Autografts (CEA) with Expanded Mesh Skin Grafting Over an Artificial Dermis or Dermal Wound Bed Preparation. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19010057"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/10\/1285\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:11:16Z","timestamp":1760112676000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/10\/1285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,11]]},"references-count":69,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["life14101285"],"URL":"https:\/\/doi.org\/10.3390\/life14101285","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,11]]}}}