{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T23:43:38Z","timestamp":1782776618537,"version":"3.54.5"},"reference-count":57,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Artificial intelligence applied to time-lapse microscopy may revolutionize embryo selection in IVF by automating data collection and standardizing the assessments. In this context, blastocyst expansion dynamics, although being associated with reproductive fitness, have been poorly studied. This retrospective study (N = 2184 blastocysts from 786 cycles) exploited both technologies to picture the association between embryo and inner-cell-mass (ICM) area in \u00b5m2, the ICM\/Trophectoderm ratio, and the zona pellucida thickness in \u00b5m (zp-T) at sequential blastocyst expansion stages, with (i) euploidy and (ii) live-birth per transfer (N = 548 transfers). A quantitative-standardized-expansion-assay (qSEA) was also set-up; a novel approach involving automatic annotations of all expansion metrics every 30 min across 5 h following blastulation. Multivariate regressions and ROC curve analyses were conducted. Aneuploid blastocysts were slower, expanded less and showed thicker zp. The qSEA outlined faster and more consistent zp thinning processes among euploid blastocysts, being more or as effective as the embryologists in ranking euploid embryo as top-quality of their cohorts in 69% of the cases. The qSEA also outlined faster and more consistent blastocyst expansion and zp thinning dynamics among euploid implanted versus not implanted blastocysts, disagreeing with embryologists\u2019 priority choice in about 50% of the cases. In conclusion, qSEA is a promising objective, quantitative, and user-friendly strategy to predict embryo competence that now deserves prospective validations.<\/jats:p>","DOI":"10.3390\/life14111396","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T09:11:24Z","timestamp":1730279484000},"page":"1396","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5390-9221","authenticated-orcid":false,"given":"Danilo","family":"Cimadomo","sequence":"first","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuele","family":"Trio","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, DEMETRA, 50141 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Canosi","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d, University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Innocenti","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Saturno","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3993-7956","authenticated-orcid":false,"given":"Marilena","family":"Taggi","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria Maria","family":"Soscia","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"},{"name":"Department of Biomedicine and Prevention, University of Tor Vergata, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Albricci","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Kantor","sequence":"additional","affiliation":[{"name":"Fairtilty Ltd., Tel Aviv 6721508, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Dvorkin","sequence":"additional","affiliation":[{"name":"Fairtilty Ltd., Tel Aviv 6721508, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Svensson","sequence":"additional","affiliation":[{"name":"Fairtilty Ltd., Tel Aviv 6721508, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Huang","sequence":"additional","affiliation":[{"name":"Pacific In Vitro Fertilization Institute, Honolulu, HI 96826, USA"},{"name":"John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96826, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Vaiarelli","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4390-9428","authenticated-orcid":false,"given":"Gianluca","family":"Gennarelli","sequence":"additional","affiliation":[{"name":"Gynecology and Obstetrics 1U, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Turin, 10126 Turin, Italy"},{"name":"IVIRMA Global Research Alliance, Livet, 10126 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rienzi","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, 00197 Rome, Italy"},{"name":"Department of Biomolecular Sciences, University \u201cCarlo Bo\u201d of Urbino, 61029 Urbino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/humupd\/dmu064","article-title":"Diagnosis of human preimplantation embryo viability","volume":"21","author":"Gardner","year":"2015","journal-title":"Hum. Reprod. 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