{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:46:00Z","timestamp":1780929960214,"version":"3.54.1"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Producing a tissue-engineered pancreas based on a tissue-specific scaffold from a decellularized pancreas, imitating the natural pancreatic tissue microenvironment and the islets of Langerhans, is one of the approaches to treating patients with type 1 diabetes mellitus (T1DM). The aim of this work was to investigate the ability of a fine-dispersed tissue-specific scaffold (DP scaffold) from decellularized human pancreas fragments to support the islets\u2019 survival and insulin-producing function when injected in a streptozotocin-induced diabetic rat model. The developed decellularization protocol allows us to obtain a scaffold with a low DNA content (33 [26; 38] ng\/mg of tissue, p &lt; 0.05) and with the preservation of GAGs (0.92 [0.84; 1.16] \u00b5g\/mg, p &lt; 0.05) and fibrillar collagen (273.7 [241.2; 303.0] \u00b5g\/mg, p &lt; 0.05). Rat islets of Langerhans were seeded in the obtained scaffolds. The rats with stable T1DM were treated by intraperitoneal injections of rat islets alone and islets seeded on the DP scaffold. The blood glucose level was determined for 10 weeks with a histological examination of experimental animals\u2019 pancreas. A more pronounced decrease in the recipient rats\u2019 glycemia was detected after comparing the islets seeded on the DP scaffold with the control injection (by 71.4% and 51.2%, respectively). It has been shown that the DP scaffold facilitates a longer survival and the efficient function of pancreatic islets in vivo and can be used to engineer a pancreas.<\/jats:p>","DOI":"10.3390\/life14111505","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T07:51:15Z","timestamp":1732002675000},"page":"1505","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Tissue-Engineered Construct Based on a Decellularized Scaffold and the Islets of Langerhans: A Streptozotocin-Induced Diabetic Model"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1995-3373","authenticated-orcid":false,"given":"Victor I.","family":"Sevastianov","sequence":"first","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"},{"name":"The Institute of Biomedical Research and Technology (IBRT), Autonomous Non-Profit Organization, 123557 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S.","family":"Ponomareva","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia V.","family":"Baranova","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-5883","authenticated-orcid":false,"given":"Aleksandra D.","family":"Belova","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyudmila A.","family":"Kirsanova","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla O.","family":"Nikolskaya","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-4016","authenticated-orcid":false,"given":"Eugenia G.","family":"Kuznetsova","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizaveta O.","family":"Chuykova","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"},{"name":"The Sechenov First Moscow State Medical University, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolay N.","family":"Skaletskiy","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina N.","family":"Skaletskaya","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniy A.","family":"Nemets","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4807-3164","authenticated-orcid":false,"given":"Yulia B.","family":"Basok","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey V.","family":"Gautier","sequence":"additional","affiliation":[{"name":"The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia"},{"name":"The Sechenov First Moscow State Medical University, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/S2213-8587(19)30412-7","article-title":"Type 1 diabetes-early life origins and changing epidemiology","volume":"8","author":"Norris","year":"2020","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1111\/j.1600-6143.2008.02353.x","article-title":"The clinical impact of islet transplantation","volume":"8","author":"Fiorina","year":"2008","journal-title":"Am. J. Transplant."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1056\/NEJM200007273430401","article-title":"Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen","volume":"343","author":"Shapiro","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.2337\/dc15-1988","article-title":"Phase 3 trial of transplantation of human islets in type 1 diabetes complicated by severe hypoglycemia","volume":"39","author":"Hering","year":"2016","journal-title":"Diabetes Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.2337\/dc12-0063","article-title":"Improvement in outcomes of clinical islet transplantation: 1999\u20132010","volume":"35","author":"Barton","year":"2012","journal-title":"Diabetes Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.2337\/diabetes.54.7.2060","article-title":"Five-year follow-up after clinical islet transplantation","volume":"54","author":"Ryan","year":"2005","journal-title":"Diabetes"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/nrendo.2016.178","article-title":"Clinical Pancreatic Islet Transplantation","volume":"13","author":"Shapiro","year":"2017","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cayabyab, F., Nih, L.R., and Yoshihara, E. (2021). Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.732431"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Eguchi, N., Damyar, K., Alexander, M., Dafoe, D., Lakey, J.R.T., and Ichii, H. (2022). Anti-Oxidative Therapy in Islet Cell Transplantation. Antioxidants, 11.","DOI":"10.3390\/antiox11061038"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1097\/01.TP.0000157273.60147.7C","article-title":"Current status of clinical islet transplantation","volume":"79","author":"Korsgren","year":"2005","journal-title":"Transplantation"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1097\/00007691-200502000-00008","article-title":"The portal immunosuppressive storm: Relevance to islet transplantation?","volume":"27","author":"Shapiro","year":"2005","journal-title":"Ther. Drug Monit."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.2337\/diabetes.48.10.1907","article-title":"Incompatibility between human blood and isolated islets of Langerhans: A finding with implications for clinical intraportal islet transplantation?","volume":"48","author":"Bennet","year":"1999","journal-title":"Diabetes"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1900\/RDS.2017.14.334","article-title":"Polymeric scaffolds for pancreatic tissue engineering: A review","volume":"14","author":"Kumar","year":"2018","journal-title":"Rev. Diabet. Stud."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"77","DOI":"10.2741\/4196","article-title":"Collagen matrix support of pancreatic islet survival and function","volume":"19","author":"Riopel","year":"2014","journal-title":"Front. Biosci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/19382014.2017.1335842","article-title":"Use of additives, scaffolds and extracellular matrix components for improvement of human pancreatic islet outcomes in vitro: A systematic review","volume":"9","author":"Lemos","year":"2017","journal-title":"Islets"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1177\/1932296813519558","article-title":"Extracellular matrix scaffold technology for bioartificial pancreas engineering: State of the art and future challenges","volume":"8","author":"Salvatori","year":"2014","journal-title":"J. Diab. Sci. Technol."},{"key":"ref_17","first-page":"765","article-title":"Scaffolds for pancreatic tissue engineering","volume":"Volume 2","author":"Mozafari","year":"2019","journal-title":"Handbook of Tissue Engineering Scaffolds"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1089\/ten.tea.2013.0765","article-title":"Tissue mimetics: Engineered hydrogel matrices provide biomimetic environments for cell growth","volume":"20","author":"Fisher","year":"2014","journal-title":"Tissue Eng. Part A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1016\/j.copbio.2013.05.004","article-title":"Engineering a local microenvironment for pancreatic islet replacement","volume":"24","author":"Coronel","year":"2013","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Abualhassan, N., Sapozhnikov, L., Pawlick, R.L., Kahana, M., Pepper, A.R., Bruni, A., Gala-Lopez, B., Kin, T., Mitrani, E., and Shapiro, A.M. (2016). Lung-derived microscaffolds facilitate diabetes reversal after mouse and human intraperitoneal islet transplantation. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156053"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s10616-017-0067-6","article-title":"Real architecture for 3D Tissue (RAFT) culture system improves viability and maintains insulin and glucagon production of mouse pancreatic islet cells","volume":"69","author":"Szebeni","year":"2017","journal-title":"Cytotechnology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/ten.teb.2013.0462","article-title":"Tissue engineering approaches to cell-based type 1 diabetes therapy","volume":"20","author":"Amer","year":"2014","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1002\/term.2655","article-title":"Decellularized pancreas as a native extracellular matrix scaffold for pancreatic islet seeding and culture","volume":"12","author":"Damodaran","year":"2018","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mendibil, U., Ruiz-Hernandez, R., Retegi-Carrion, S., Garcia-Urquia, N., Olalde-Graells, B., and Abarrategi, A. (2020). Tissue-specific decellularization methods: Rationale and strategies to achieve regenerative compounds. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155447"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1002\/term.2061","article-title":"Development of decellularized scaffolds for stem cell-driven tissue engineering","volume":"11","author":"Rana","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Tripathi, A., and Melo, J.S. (2017). Decellularized tissue engineering. Advances in Biomaterials for Biomedical Applications, Springer.","DOI":"10.1007\/978-981-10-3328-5"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1002\/jbm.a.37474","article-title":"Decellularization of cartilage microparticles: Effects of temperature, supercritical carbon dioxide and ultrasound on biochemical, mechanical, and biological properties","volume":"111","author":"Sevastianov","year":"2023","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5488","DOI":"10.1016\/j.biomaterials.2013.03.054","article-title":"Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering","volume":"34","author":"Orlando","year":"2013","journal-title":"Biomaterials"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.actbio.2022.11.032","article-title":"Tissue engineering of decellularized pancreas scaffolds for regenerative medicine in diabetes","volume":"157","author":"Lim","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sevastianov, V.I., Ponomareva, A.S., Baranova, N.V., Kirsanova, L.A., Basok, Y.B., Nemets, E.A., Kruglov, D.N., Miloserdov, I.A., and Gautier, S.V. (2023). Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010119"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wu, D., Wan, J., Huang, Y., Guo, Y., Xu, T., Zhu, M., Fan, X., Zhu, S., Ling, C., and Li, X. (2015). 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. Biomed. Res. Int., 2015.","DOI":"10.1155\/2015\/432645"},{"key":"ref_32","first-page":"1055","article-title":"Research Progress on Animal Models of Diabetes Mellitus","volume":"10","author":"Zhang","year":"2023","journal-title":"Austin J. Cardiovasc. Dis. Atheroscler."},{"key":"ref_33","first-page":"17","article-title":"Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets","volume":"3","author":"Sevastianov","year":"2021","journal-title":"J. Genet. Eng. Biotechnol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1038\/cddis.2010.14","article-title":"Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells","volume":"1","author":"Karaoz","year":"2010","journal-title":"Cell Death Dis."},{"key":"ref_35","unstructured":"(2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5:2009)."},{"key":"ref_36","unstructured":"(2009). Medical Devices. Assessment of the Biological Effect of Medical Devices\u2014Part 2. Requirements for the Treatment of Animals (Standard No. ISO 10993-2:2009)."},{"key":"ref_37","unstructured":"(2016). Guidelines for the Accommodation and Care of Laboratory Animals (Standard No. GOST 33215-2014)."},{"key":"ref_38","unstructured":"(2016). Guidelines for the Accommodation and Care of Laboratory Animals (Standard No. GOST 33216-2014)."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1016\/j.biomaterials.2011.01.057","article-title":"An overview of tissue and whole organ decellularization processes","volume":"32","author":"Crapo","year":"2011","journal-title":"Biomaterials"},{"key":"ref_40","first-page":"200","article-title":"Isolation, culture and functional evaluation of islets of Langerhans","volume":"24","author":"London","year":"1998","journal-title":"Diabetes Metab."},{"key":"ref_41","unstructured":"Sevastianov, V.I., and Basok, Y.B. (2023). Biomimetics of Extracellular Matrices for Cell and Tissue Engineered Medical Products, Cambridge Scholars Publishing."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Sackett, S.D., Tremmel, D.M., Ma, F., Feeney, A.K., Maguire, R.M., Brown, M.E., Zhou, Y., Li, X., O\u2019Brien, C., and Li, L. (2018). Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-28857-1"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s11892-018-1014-4","article-title":"Therapeutic strategies for modulating the extracellular matrix to improve pancreatic islet function and survival after transplantation","volume":"18","author":"Smink","year":"2018","journal-title":"Curr. Diab. Rep."},{"key":"ref_44","unstructured":"Casale, J., and Crane, J.S. (2023). Biochemistry, Glycosaminoglycans. StatPearls, StatPearls Publishing."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Lepedda, A.J., Nieddu, G., Formato, M., Baker, M.B., Fern\u00e1ndez-P\u00e9rez, J., and Moroni, L. (2021). Glycosaminoglycans: From Vascular Physiology to Tissue Engineering Applications. Front. Chem., 9.","DOI":"10.3389\/fchem.2021.680836"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.msec.2019.04.002","article-title":"Decellularized cartilage as a prospective scaffold for cartilage repair","volume":"101","author":"Xia","year":"2019","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1089\/ten.tec.2016.0380","article-title":"Systematic comparison of protocols for the preparation of human articular cartilage for use as scaffold material in cartilage tissue engineering","volume":"22","author":"Schneider","year":"2016","journal-title":"Tissue Eng. Part. C Methods"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bautista, C.A., Park, H.J., Mazur, C.M., Aaron, R.K., and Bilgen, B. (2016). Effects of chondroitinase ABC-mediated proteoglycan digestion on decellularization and recellularization of articular cartilage. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0158976"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Dubus, M., Scomazzon, L., Chevrier, J., Ledouble, C., Baldit, A., Braux, J., Gindraux, F., Boulagnon, C., Audonnet, S., and Colin, M. (2022). Antibacterial and Immunomodulatory Properties of Acellular Wharton\u2019s Jelly Matrix. Biomedicines, 10.","DOI":"10.3390\/biomedicines10020227"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1242\/dev.114215","article-title":"Orchestrating liver development","volume":"142","author":"Gordillo","year":"2015","journal-title":"Development"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1152\/physrev.00008.2017","article-title":"Pancreatic beta-cell electrical activity and insulin secretion: Of mice and men","volume":"98","author":"Rorsman","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"145","DOI":"10.5152\/eurasianjmed.2022.22304","article-title":"Experimental approaches to diabetes mellitus","volume":"54","author":"Yayla","year":"2022","journal-title":"Eurasian J. Med."},{"key":"ref_53","first-page":"252","article-title":"Induction of type-1 diabetes mellitus in laboratory rats by use of alloxan: Route of administration, pitfalls, and insulin treatment","volume":"54","author":"Federiuk","year":"2004","journal-title":"Comp. Med."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Zhang, J., Hong, M., Huang, J., Wang, R., Tan, B., Huang, P., and Cao, H. (2021). Study on the Optimization and Stability of Single-dose Streptozotocin-induced Diabetic Modelling in Rats. Res. Sq., preprint.","DOI":"10.21203\/rs.3.rs-310098\/v1"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e15533","DOI":"10.1016\/j.heliyon.2023.e15533","article-title":"Self-recovery in diabetic Sprague Dawley rats induced by intraperitoneal alloxan and streptozotocin","volume":"9","author":"Fajarwati","year":"2023","journal-title":"Heliyon"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.biomaterials.2017.05.033","article-title":"A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice","volume":"138","author":"Wang","year":"2017","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Yu, H., Chen, Y., Kong, H., He, Q., Sun, H., Bhugul, P.A., Zhang, Q., Chen, B., and Zhou, M. (2018). The rat pancreatic body tail as a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering. J. Biol. Eng., 12.","DOI":"10.1186\/s13036-018-0096-5"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s13770-020-00296-y","article-title":"Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas","volume":"18","author":"Tripathi","year":"2021","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.biomaterials.2019.01.035","article-title":"Biofabrication of a vascularized islet organ for type 1 diabetes","volume":"199","author":"Citro","year":"2019","journal-title":"Biomaterials"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/11\/1505\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:35:17Z","timestamp":1760114117000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/11\/1505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":59,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["life14111505"],"URL":"https:\/\/doi.org\/10.3390\/life14111505","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]}}}