{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:48:36Z","timestamp":1791402516394,"version":"4.3.3"},"reference-count":64,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Financiamiento de Proyectos de Investigaci\u00f3n para la Salud (FPIS 2023), de la Direcci\u00f3n General de Pol\u00edticas de Investigaci\u00f3n en Salud (DGPIS)","award":["INERICV-4854"],"award-info":[{"award-number":["INERICV-4854"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Exosome-derived microRNAs (miRNAs) are potential biomarkers for lung cancer detection and monitoring through liquid biopsy. These small, non-coding RNA molecules are found within exosomes, which are extracellular vesicles released from cells. Their stability in biofluids, such as blood, positions them as candidates for minimally invasive diagnostics. Multiple studies have shown that lung cancer patients exhibit distinct miRNA profiles compared to healthy individuals. This finding suggests that exosome-derived miRNAs could serve as valuable biomarkers for diagnosis, prognosis, and evaluating therapeutic responses. This review summarizes recent research on exosome-derived miRNAs in liquid biopsies, including blood, pleural effusion, and pleural lavage, as biomarkers for lung cancer, focusing on publications from the last five years.<\/jats:p>","DOI":"10.3390\/life14121608","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T04:13:03Z","timestamp":1733371983000},"page":"1608","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Exosome-Derived miRNAs in Liquid Biopsy for Lung Cancer"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6881-4655","authenticated-orcid":false,"given":"Israel","family":"Mart\u00ednez-Espinosa","sequence":"first","affiliation":[{"name":"Department of Molecular Biomedicine and Translational Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cos\u00edo Villegas, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4252-2638","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Serrato","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedicine and Translational Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cos\u00edo Villegas, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3192-8915","authenticated-orcid":false,"given":"Carlos","family":"Cabello-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Department of Research in Virology and Mycology, Instituto Nacional de Enfermedades Respiratorias Ismael Cos\u00edo Villegas, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngeles","family":"Carlos-Reyes","sequence":"additional","affiliation":[{"name":"Laboratory of Onco-Immunobiology, Instituto Nacional de Enfermedades Respiratorias Ismael Cos\u00edo Villegas, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6298-0073","authenticated-orcid":false,"given":"Blanca","family":"Ortiz-Quintero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedicine and Translational Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cos\u00edo Villegas, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO), and International Agency for Research of Cancer (IARC) (2024, September 06). GLOBOCAN 2020, Section of Cancer Surveillance. Available online: http:\/\/globocan.iarc.fr."},{"key":"ref_2","unstructured":"American Cancer Society (2024, September 06). Lung Cancer Survival Rates. Available online: https:\/\/www.cancer.org\/cancer\/types\/lung-cancer\/detection-diagnosis-staging\/survival-rates.html."},{"key":"ref_3","unstructured":"International Agency for Research on Cancer (iarc) WHO (2024, August 26). Cancer Today. Available online: http:\/\/gco.iarc.fr\/today\/home."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1186\/s12935-023-02990-y","article-title":"Recent advances in non-small cell lung cancer targeted therapy; an update review","volume":"23","author":"Araghi","year":"2023","journal-title":"Cancer Cell Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1002\/cac2.12505","article-title":"Management of locally advanced non-small cell lung cancer: State of the art and future directions","volume":"44","author":"Miao","year":"2024","journal-title":"Cancer Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s12964-023-01139-8","article-title":"Radiation combined with immune checkpoint inhibitors for unresectable locally advanced non-small cell lung cancer: Synergistic mechanisms, current state, challenges, and orientations","volume":"21","author":"Wu","year":"2023","journal-title":"Cell Commun. Signal"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12943-022-01505-z","article-title":"Liquid biopsy in lung cancer: Significance in diagnostics, prediction, and treatment monitoring","volume":"21","author":"Li","year":"2022","journal-title":"Mol. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s13046-024-03026-7","article-title":"Liquid biopsy techniques and lung cancer: Diagnosis, monitoring and evaluation","volume":"43","author":"Ren","year":"2024","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1093\/annonc\/mdz227","article-title":"How liquid biopsies can change clinical practice in oncology","volume":"30","author":"Siravegna","year":"2019","journal-title":"Ann. Oncol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/s41571-020-00457-x","article-title":"Liquid biopsy enters the clinic-implementation issues and future challenges","volume":"18","author":"Ignatiadis","year":"2021","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s12967-023-03960-8","article-title":"Liquid biopsies: The future of cancer early detection","volume":"21","author":"Connal","year":"2023","journal-title":"J. Transl. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.tips.2019.01.006","article-title":"Liquid Biopsies in Cancer Diagnosis, Monitoring, and Prognosis","volume":"40","author":"Bebawy","year":"2019","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.annonc.2021.01.074","article-title":"Exosome-based liquid biopsies in cancer: Opportunities and challenges","volume":"32","author":"Yu","year":"2021","journal-title":"Ann. Oncol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12967-021-03215-4","article-title":"The role of Exosomal miRNAs in cancer","volume":"20","author":"Li","year":"2022","journal-title":"J. Transl. Med."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ortiz-Quintero, B. (2020). Extracellular MicroRNAs as Intercellular Mediators and Noninvasive Biomarkers of Cancer. Cancers, 12.","DOI":"10.3390\/cancers12113455"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Xu, D., Di, K., Fan, B., Wu, J., Gu, X., Sun, Y., Khan, A., Li, P., and Li, Z. (2022). MicroRNAs in extracellular vesicles: Sorting mechanisms, diagnostic value, isolation, and detection technology. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.948959"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5311","DOI":"10.1158\/1078-0432.CCR-17-0577","article-title":"Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing","volume":"23","author":"Jin","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e000376","DOI":"10.1136\/jitc-2019-000376","article-title":"Correlation of plasma exosomal microRNAs with the efficacy of immunotherapy in EGFR\/ALK wild-type advanced non-small cell lung cancer","volume":"8","author":"Peng","year":"2020","journal-title":"J. Immunother. Cancer"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Kim, D.H., Park, H., Choi, Y.J., Im, K., Lee, C.W., Kim, D.S., Pack, C.G., Kim, H.Y., Choi, C.M., and Lee, J.C. (2023). Identification of exosomal microRNA panel as diagnostic and prognostic biomarker for small cell lung cancer. Biomark. Res., 11.","DOI":"10.1186\/s40364-023-00517-1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.semcancer.2019.08.017","article-title":"Extracellular vesicles in cancer nanomedicine","volume":"69","author":"Tarasov","year":"2021","journal-title":"Semin. Cancer Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"D516","DOI":"10.1093\/nar\/gky1029","article-title":"Vesiclepedia 2019: A compendium of RNA, proteins, lipids and metabolites in extracellular vesicles","volume":"47","author":"Pathan","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.semcdb.2017.08.022","article-title":"Biogenesis and function of ESCRT-dependent extracellular vesicles","volume":"74","author":"Juan","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00018-017-2595-9","article-title":"Current knowledge on exosome biogenesis and release","volume":"75","author":"Hessvik","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41556-018-0250-9","article-title":"Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication","volume":"21","author":"Mathieu","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41576-023-00611-y","article-title":"microRNAs in action: Biogenesis, function and regulation","volume":"24","author":"Shang","year":"2023","journal-title":"Nat. Rev. Genet."},{"key":"ref_27","first-page":"1","article-title":"Recent progress in miRNA biogenesis and decay","volume":"21","year":"2024","journal-title":"RNA Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.molcel.2014.11.030","article-title":"Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis","volume":"57","author":"Wilson","year":"2015","journal-title":"Mol. Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.gpb.2017.09.004","article-title":"The Role of Exportin-5 in MicroRNA Biogenesis and Cancer","volume":"16","author":"Wu","year":"2018","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1126\/science.1258040","article-title":"Structural basis for microRNA targeting","volume":"346","author":"Schirle","year":"2014","journal-title":"Science"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10849","DOI":"10.1074\/jbc.M112.446831","article-title":"Neutral sphingomyelinase 2 (nSMase2)-dependent exosomal transfer of angiogenic microRNAs regulate cancer cell metastasis","volume":"288","author":"Kosaka","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.celrep.2014.08.027","article-title":"Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes","volume":"8","author":"Hackenberg","year":"2014","journal-title":"Cell Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1038\/ncomms3980","article-title":"Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs","volume":"4","author":"Vazquez","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Martinez-Espinosa, I., Serrato, J.A., and Ortiz-Quintero, B. (2023). The Role of Exosome-Derived microRNA on Lung Cancer Metastasis Progression. Biomolecules, 13.","DOI":"10.3390\/biom13111574"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Martinez-Espinosa, I., Serrato, J.A., and Ortiz-Quintero, B. (2024). MicroRNAs in Lung Cancer Brain Metastasis. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms251910325"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1038\/s41419-021-04024-9","article-title":"Exosomal miR-1260b derived from non-small cell lung cancer promotes tumor metastasis through the inhibition of HIPK2","volume":"12","author":"Kim","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1186\/s12935-021-02197-z","article-title":"MicroRNA-550a-3-5p controls the brain metastasis of lung cancer by directly targeting YAP1","volume":"21","author":"Wei","year":"2021","journal-title":"Cancer Cell Int."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1007\/s13577-022-00766-6","article-title":"Pirfenidone promotes the levels of exosomal miR-200 to down-regulate ZEB1 and represses the epithelial-mesenchymal transition of non-small cell lung cancer cells","volume":"35","author":"Liu","year":"2022","journal-title":"Hum. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1016\/j.cmet.2021.09.002","article-title":"Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming","volume":"33","author":"Morrissey","year":"2021","journal-title":"Cell Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1186\/s13046-021-01979-7","article-title":"Hypoxic tumor-derived exosomal miR-31-5p promotes lung adenocarcinoma metastasis by negatively regulating SATB2-reversed EMT and activating MEK\/ERK signaling","volume":"40","author":"Yu","year":"2021","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.yexcr.2019.03.035","article-title":"Exosomal miR-499a-5p promotes cell proliferation, migration and EMT via mTOR signaling pathway in lung adenocarcinoma","volume":"379","author":"He","year":"2019","journal-title":"Exp. Cell Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Liu, Y., Su, C.Y., Yan, Y.Y., Wang, J., Li, J.J., Fu, J.J., Wang, Y.Q., and Zhang, J.Y. (2022). Exosomes of A549 Cells Induced Migration, Invasion, and EMT of BEAS-2B Cells Related to let-7c-5p and miR-181b-5p. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.926769"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/nrclinonc.2017.14","article-title":"Integrating liquid biopsies into the management of cancer","volume":"14","author":"Siravegna","year":"2017","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.7150\/thno.41580","article-title":"Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics","volume":"10","author":"Yang","year":"2020","journal-title":"Theranostics"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Chen, J., Li, P., Zhang, T., Xu, Z., Huang, X., Wang, R., and Du, L. (2021). Review on Strategies and Technologies for Exosome Isolation and Purification. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.811971"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hassanin, A.A.I., and Ramos, K.S. (2024). Circulating Exosomal miRNA Profiles in Non-Small Cell Lung Cancers. Cells, 13.","DOI":"10.3390\/cells13181562"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/s10238-024-01427-8","article-title":"Extracellular vesicles miR-574-5p and miR-181a-5p as prognostic markers in NSCLC patients treated with nivolumab","volume":"24","author":"Genova","year":"2024","journal-title":"Clin. Exp. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e21059","DOI":"10.1016\/j.heliyon.2023.e21059","article-title":"Plasma exosomal miR-1290 and miR-29c-3p as diagnostic biomarkers for lung cancer","volume":"9","author":"Zhang","year":"2023","journal-title":"Heliyon"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.7150\/ijms.43500","article-title":"Tumor-derived circulating exosomal miR-342-5p and miR-574-5p as promising diagnostic biomarkers for early-stage Lung Adenocarcinoma","volume":"17","author":"Han","year":"2020","journal-title":"Int. J. Med. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1177\/15353702231184223","article-title":"Circulating exosomal microRNA-4497 as a potential biomarker for metastasis and prognosis in non-small-cell lung cancer","volume":"248","author":"Zheng","year":"2023","journal-title":"Exp. Biol. Med."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Tang, Y., Song, X., Xie, L., Zhao, S., and Song, X. (2020). Tumor-Derived Exosomal miRNAs as Diagnostic Biomarkers in Non-Small Cell Lung Cancer. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.560025"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3382","DOI":"10.1002\/jcb.29612","article-title":"Serum exosomal miR-7977 as a novel biomarker for lung adenocarcinoma","volume":"121","author":"Chen","year":"2020","journal-title":"J. Cell. Biochem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e23237","DOI":"10.1002\/jcla.23237","article-title":"Serum exosomal miR-378 upregulation is associated with poor prognosis in non-small-cell lung cancer patients","volume":"34","author":"Zhang","year":"2020","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1515\/cclm-2019-1329","article-title":"Tumor-associated exosomal miRNA biomarkers to differentiate metastatic vs. nonmetastatic non-small cell lung cancer","volume":"58","author":"Wang","year":"2020","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1186\/s12935-021-02075-8","article-title":"Elevated exosome-derived miRNAs predict osimertinib resistance in non-small cell lung cancer","volume":"21","author":"Li","year":"2021","journal-title":"Cancer Cell Int."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1016\/j.jtho.2020.05.022","article-title":"Circulating MicroRNAs and Extracellular Vesicle-Containing MicroRNAs as Response Biomarkers of Anti-programmed Cell Death Protein 1 or Programmed Death-Ligand 1 Therapy in NSCLC","volume":"15","author":"Shukuya","year":"2020","journal-title":"J. Thorac. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3233\/CBM-203075","article-title":"Exosomal microRNAs as potential biomarkers for osimertinib resistance of non-small cell lung cancer patients","volume":"31","author":"Janpipatkul","year":"2021","journal-title":"Cancer Biomark."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/1759-7714.12756","article-title":"Diagnostic value of microRNAs derived from exosomes in bronchoalveolar lavage fluid of early-stage lung adenocarcinoma: A pilot study","volume":"9","author":"Kim","year":"2018","journal-title":"Thorac. Cancer"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.cca.2015.07.027","article-title":"Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer","volume":"450","author":"Rehbein","year":"2015","journal-title":"Clin. Chim. Acta"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Zhang, X., Bao, L., Yu, G., and Wang, H. (2022). Exosomal miRNA-profiling of pleural effusion in lung adenocarcinoma and tuberculosis. Front. Surg., 9.","DOI":"10.3389\/fsurg.2022.1050242"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1002\/cam4.2928","article-title":"Clinicopathological significance of microRNA-21 in extracellular vesicles of pleural lavage fluid of lung adenocarcinoma and its functions inducing the mesothelial to mesenchymal transition","volume":"9","author":"Watabe","year":"2020","journal-title":"Cancer Med."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Roman-Canal, B., Moiola, C.P., Gatius, S., Bonnin, S., Ruiz-Miro, M., Gonzalez, E., Ojanguren, A., Recuero, J.L., Gil-Moreno, A., and Falcon-Perez, J.M. (2019). EV-associated miRNAs from pleural lavage as potential diagnostic biomarkers in lung cancer. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-51578-y"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e12404","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/12\/1608\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:47:06Z","timestamp":1760114826000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/14\/12\/1608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,4]]},"references-count":64,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["life14121608"],"URL":"https:\/\/doi.org\/10.3390\/life14121608","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,4]]}}}