{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T01:35:52Z","timestamp":1778031352373,"version":"3.51.4"},"reference-count":80,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"the National Research Council of Thailand","doi-asserted-by":"publisher","award":["NRCT5-TRG63002-01"],"award-info":[{"award-number":["NRCT5-TRG63002-01"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Kasetsart University Research and Development (KURDI)","award":["NRCT5-TRG63002-01"],"award-info":[{"award-number":["NRCT5-TRG63002-01"]}]},{"name":"Institute of Food Research and Product Development (IFRPD), Kasetsart University, Thailand","award":["NRCT5-TRG63002-01"],"award-info":[{"award-number":["NRCT5-TRG63002-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Microalgae show promise as a biomass and bioproduct for applications in various industries. The cultivation of microalgae plays a crucial role in optimizing biomass yield and bioproduct accumulation. The provision of inorganic carbon substrates substantially enhances microalgal growth and lipid biosynthesis, resulting in marked increases in the production of biofuels and other bioproducts. This study examined biomass and lipid accumulation in Messastrum gracile IFRPD 1061 under inorganic stress conditions, previously unreported. M. gracile IFRPD 1061 was subjected to varying conditions of inorganic carbon substrates, 1\u20133 g\u00b7L\u22121 sodium carbonate and bicarbonate concentration, to enhance biomass and lipid accumulation. Optimal productivity levels were observed with sodium bicarbonate addition of 3 g\u00b7L\u22121 and 1 g\u00b7L\u22121 for biomass and lipids, resulting in productivities of 392.64 and 53.57 mg\u00b7L\u22121\u00b7d\u22121, respectively. Results underlined the effectiveness of sodium carbonate and bicarbonate as inorganic carbon sources for stimulating microalgal growth and enhancing the production of high-value products. The extraction of lipids from freeze-dried biomass of M. gracile IFRPD 1061 demonstrated optimal yield using methanol\/hexane solvents compared with the control experiments. Lipid extraction yields using methanol\/hexane were 42.18% and 46.81% from oven-dried and freeze-dried biomass, respectively. Lipids extracted from oven-dried M. gracile IFRPD 1061 using methanol\/hexane\/chloroform solvents indicated the potential of methanol\/hexane as a solvent for lipid extraction from dry microalgal biomass using an ultrasonic-assisted technique. This study contributes valuable insights into maximizing biofuel and bioproduct production from microalgae, highlighting A. gracilis as a promising candidate for industrial applications.<\/jats:p>","DOI":"10.3390\/life15030407","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T07:56:52Z","timestamp":1741161412000},"page":"407","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enhancing Biomass and Lipid Production in Messastrum gracile Using Inorganic Carbon Substrates and Alternative Solvents for Lipid Extraction"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5392-8302","authenticated-orcid":false,"given":"Wanida","family":"Pan-utai","sequence":"first","affiliation":[{"name":"Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soisuda","family":"Pornpukdeewattana","sequence":"additional","affiliation":[{"name":"School of Food Industry, King Mongkut\u2019s Institute of Technology Ladkrabang, Bangkok 10520, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilasinee","family":"Inrung","sequence":"additional","affiliation":[{"name":"School of Food Industry, King Mongkut\u2019s Institute of Technology Ladkrabang, Bangkok 10520, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theera","family":"Thurakit","sequence":"additional","affiliation":[{"name":"Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-1390","authenticated-orcid":false,"given":"Penjit","family":"Srinophakun","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"138447","DOI":"10.1016\/j.chemosphere.2023.138447","article-title":"Emerging microalgae-based biofuels: Technology, life-cycle and scale-up","volume":"326","author":"Marangon","year":"2023","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sundaram, T., Rajendran, S., Gnanasekaran, L., Rachmadona, N., Jiang, J.J., Khoo, K.S., and Show, P.L. (2023). Bioengineering strategies of microalgae biomass for biofuel production: Recent advancement and insight. Bioengineered, 14.","DOI":"10.1080\/21655979.2023.2252228"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/s11101-021-09784-y","article-title":"Production of microalgae with high lipid content and their potential as sources of nutraceuticals","volume":"22","author":"Udayan","year":"2023","journal-title":"Phytochem. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sar\u0131ta\u015f, S., Kalkan, A.E., Y\u0131lmaz, K., Gurdal, S., G\u00f6ksan, T., Witkowska, A.M., Lombardo, M., and Karav, S. (2025). Biological and nutritional applications of microalgae. Nutrients, 17.","DOI":"10.3390\/nu17010093"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12934-018-0879-x","article-title":"The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products","volume":"17","author":"Khan","year":"2018","journal-title":"Microb. Cell Factories"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Tomar, S., Agarwal, S., Singh, H., Kumar, R., Qureshi, K.A., Jaremko, M., Emwas, A.-H., and Rai, P.K. (2023). Microalgae: A promising source for biofuel production. Biocatal. Agric. Biotechnol., 53.","DOI":"10.1016\/j.bcab.2023.102877"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"100367","DOI":"10.1016\/j.envc.2021.100367","article-title":"Biodiesel potentials of microalgal strains isolated from fresh water environment","volume":"5","author":"Azeez","year":"2021","journal-title":"Environ. Chall."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1007\/s12667-022-00514-7","article-title":"A review on the current status and post-pandemic prospects of third-generation biofuels","volume":"14","author":"Maliha","year":"2023","journal-title":"Energy Syst."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhu, Z., Sun, J., Fa, Y., Liu, X., and Lindblad, P. (2022). Enhancing microalgal lipid accumulation for biofuel production. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1024441"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wang, L., Chen, L., Yang, S., and Tan, X. (2020). Photosynthetic conversion of carbon dioxide to oleochemicals by cyanobacteria: Recent advances and future perspectives. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00634"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/15567249.2015.1052594","article-title":"Oil production from pilot-scale microalgae cultivation: An economics evaluation","volume":"11","author":"Zhu","year":"2016","journal-title":"Energy Sources Part B Econ. Plan. Policy"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1186\/s12934-019-1228-4","article-title":"Biomass and lipid induction strategies in microalgae for biofuel production and other applications","volume":"18","author":"Rafiei","year":"2019","journal-title":"Microb. Cell Factories"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101596","DOI":"10.1016\/j.eti.2021.101596","article-title":"Insights into the influence of CO2 supplement on phycoremediation and lipid accumulation potential of microalgae: An exploration for biodiesel production","volume":"23","author":"Saranya","year":"2021","journal-title":"Environ. Technol. Innov."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Feng, P., Xu, Z., Qin, L., Alam, M.A., Wang, Z., and Zhu, S. (2020). Effects of different nitrogen sources and light paths of flat plate photobioreactors on the growth and lipid accumulation of Chlorella sp. GN1 outdoors. Bioresour. Technol., 301.","DOI":"10.1016\/j.biortech.2020.122762"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"118598","DOI":"10.1016\/j.fuel.2020.118598","article-title":"Role of nanoparticles on microalgal cultivation: A review","volume":"280","author":"Longoria","year":"2020","journal-title":"Fuel"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.algal.2017.11.004","article-title":"Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana","volume":"28","author":"Qiu","year":"2017","journal-title":"Algal Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Goswami, R.K., Mehariya, S., Karthikeyan, O.P., and Verma, P. (2022). Influence of Carbon Sources on Biomass and Biomolecule Accumulation in Picochlorum sp. Cultured under the Mixotrophic Condition. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19063674"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.gce.2023.10.004","article-title":"Microalgae cultivation in photobioreactors: Sustainable solutions for a greener future","volume":"5","author":"Bahar","year":"2024","journal-title":"Green Chem. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Daneshvar, E., Sik Ok, Y., Tavakoli, S., Sarkar, B., Shaheen, S.M., Hong, H., Luo, Y., Rinklebe, J., Song, H., and Bhatnagar, A. (2021). Insights into upstream processing of microalgae: A review. Bioresour. Technol., 329.","DOI":"10.1016\/j.biortech.2021.124870"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sun, X.-M., Ren, L.-J., Zhao, Q.-Y., Ji, X.-J., and Huang, H. (2018). Microalgae for the production of lipid and carotenoids: A review with focus on stress regulation and adaptation. Biotechnol. Biofuels, 11.","DOI":"10.1186\/s13068-018-1275-9"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liu, T., Chen, Z., Xiao, Y., Yuan, M., Zhou, C., Liu, G., Fang, J., and Yang, B. (2022). Biochemical and Morphological Changes Triggered by Nitrogen Stress in the Oleaginous Microalga Chlorella vulgaris. Microorganisms, 10.","DOI":"10.3390\/microorganisms10030566"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s13201-021-01363-y","article-title":"Effects of nitrogen depletion on the biosorption capacities of Neochloris minuta and Neochloris alveolaris for five heavy metals","volume":"11","author":"Giarikos","year":"2021","journal-title":"Appl. Water Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"106355","DOI":"10.1016\/j.resconrec.2022.106355","article-title":"Overview on stress-induced strategies for enhanced microalgae lipid production: Application, mechanisms and challenges","volume":"183","author":"Song","year":"2022","journal-title":"Resour. Conserv. Recycl."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s12934-018-0953-4","article-title":"Effects of sodium bicarbonate on cell growth, lipid accumulation, and morphology of Chlorella vulgaris","volume":"17","author":"Li","year":"2018","journal-title":"Microb. Cell Fact"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cordoba-Perez, M., and de Lasa, H. (2021). CO2-Derived Carbon Capture Using Microalgae and Sodium Bicarbonate in a PhotoBioCREC Unit: Kinetic Modeling. Processes, 9.","DOI":"10.3390\/pr9081296"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1475-2859-13-14","article-title":"A comparative study: The impact of different lipid extraction methods on current microalgal lipid research","volume":"13","author":"Li","year":"2014","journal-title":"Microb. Cell Factories"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s12257-017-0392-9","article-title":"Lipid Extraction from Tetraselmis sp. Microalgae for Biodiesel Production Using Hexane-based Solvent Mixtures","volume":"23","author":"Shin","year":"2018","journal-title":"Biotechnol. Bioprocess Eng."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.biotechadv.2012.01.001","article-title":"Extraction of oil from microalgae for biodiesel production: A review","volume":"30","author":"Halim","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"101729","DOI":"10.1016\/j.algal.2019.101729","article-title":"Obtaining highly pure EPA-rich lipids from dry and wet Nannochloropsis gaditana microalgal biomass using ethanol, hexane and acetone","volume":"45","author":"Grima","year":"2020","journal-title":"Algal Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5591","DOI":"10.1007\/s11837-023-05897-1","article-title":"A comprehensive study on extracellular green synthesis, antibacterial activity and process design of metallic nanoparticles from Botryococcus braunii microalga","volume":"75","year":"2023","journal-title":"JOM"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Pan-utai, W., Srinophakun, P., and Inrung, W. (2019). Nutrients formulation to maximize Ankistrodesmus sp. microalgal cell biomass and lipid productivities. J. Biol. Res. Boll. Della Soc. Ital. Biol. Sper., 92.","DOI":"10.4081\/jbr.2019.8547"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.algal.2012.05.003","article-title":"Relationship between hydrocarbons and molecular phylogeny of Botryococcus braunii","volume":"1","author":"Kawachi","year":"2012","journal-title":"Algal Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1126\/science.1189003","article-title":"An outlook on microalgal biofuels","volume":"329","author":"Wijffels","year":"2010","journal-title":"Science"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"136217","DOI":"10.1016\/j.jclepro.2023.136217","article-title":"A cleaner and smarter way to achieve high microalgal biomass density coupled with facilitated self-flocculation by utilizing bicarbonate as a source of dissolved carbon dioxide","volume":"391","author":"Mitra","year":"2023","journal-title":"J. Clean. Prod."},{"key":"ref_36","unstructured":"Rice, E.W., Bridgewater, L., and Association, A.P.H. (2012). Standard Methods for the Examination of Water and Wastewater, American Public Health Association."},{"key":"ref_37","first-page":"225","article-title":"Photoautotrophic Cultivation of Arthrospira maxima for Protein Accumulation under Minimum Nutrient Availability","volume":"7","author":"Poopat","year":"2020","journal-title":"Appl. Food Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.biortech.2018.07.121","article-title":"Multi-factor kinetic modelling of microalgal biomass cultivation for optimised lipid production","volume":"269","author":"Bekirogullari","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1016\/j.jksus.2018.05.026","article-title":"Physical extraction and extrusion entrapment of C-phycocyanin from Arthrospira platensis","volume":"31","author":"Iamtham","year":"2019","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"132236","DOI":"10.1016\/j.foodchem.2022.132236","article-title":"Extraction of lipids from microalgae using classical and innovative approaches","volume":"384","author":"Zhou","year":"2022","journal-title":"Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Wenk, M.R. (2016). Liquid Extraction: Bligh and Dyer. Encyclopedia of Lipidomics, Springer.","DOI":"10.1007\/978-94-007-7864-1"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"125079","DOI":"10.1016\/j.chemosphere.2019.125079","article-title":"Algae as green energy reserve: Technological outlook on biofuel production","volume":"242","author":"Anto","year":"2020","journal-title":"Chemosphere"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Shi, T.Q., Wang, L.R., Zhang, Z.X., Sun, X.M., and Huang, H. (2020). Stresses as First-Line Tools for Enhancing Lipid and Carotenoid Production in Microalgae. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00610"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Singh, S.K., Sundaram, S., and Kishor, K. (2014). Carbon-Concentrating Mechanism of Microalgae. Photosynthetic Microorganisms: Mechanism For Carbon Concentration, Springer International Publishing.","DOI":"10.1007\/978-3-319-09123-5"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hwangbo, K., Lim, J.-M., Jeong, S.-W., Vikramathithan, J., Park, Y.-I., and Jeong, W.-J. (2018). Elevated inorganic carbon concentrating mechanism confers tolerance to high light in an arctic Chlorella sp. ArM0029B. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.00590"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1111\/pbi.12497","article-title":"Introducing an algal carbon-concentrating mechanism into higher plants: Location and incorporation of key components","volume":"14","author":"Atkinson","year":"2016","journal-title":"Plant Biotechnol. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.biortech.2019.03.024","article-title":"Effect of light conditions on mixotrophic cultivation of green microalgae","volume":"282","author":"Patel","year":"2019","journal-title":"Bioresour. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"116152","DOI":"10.1016\/j.watres.2020.116152","article-title":"Experimental assessment and mathematical modelling of the growth of Chlorella vulgaris under photoautotrophic, heterotrophic and mixotrophic conditions","volume":"184","author":"Manhaeghe","year":"2020","journal-title":"Water Res."},{"key":"ref_49","first-page":"111","article-title":"Sodium bicarbonate as inorganic carbon source for higher biomass and lipid production integrated carbon capture in Chlorella vulgaris","volume":"10","author":"Mokashi","year":"2016","journal-title":"Achiev. Life Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"114095","DOI":"10.1016\/j.jenvman.2021.114095","article-title":"Regulation of carbon source metabolism in mixotrophic microalgae cultivation in response to light intensity variation","volume":"302","author":"Gao","year":"2022","journal-title":"J. Environ. Manag."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Bhandari, M., Kharkwal, S., and Prajapati, S.K. (2024). Bicarbonate-assisted microalgae cultivation for drinking water RO reject recycling and bioproduct generation. Bioresour. Technol. Rep., 25.","DOI":"10.1016\/j.biteb.2023.101756"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1007\/s12649-023-02053-3","article-title":"Production of Metabolites in Microalgae Under Alkali Halophilic Growth Medium Using a Dissolved Inorganic Carbon Source","volume":"14","author":"Roy","year":"2023","journal-title":"Waste Biomass Valorization"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.biortech.2014.08.086","article-title":"Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus\u2013A potential strain for bio-fuel production","volume":"171","author":"George","year":"2014","journal-title":"Bioresour. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wehr, J.D., and Sheath, R.G. (2003). 7-Nonmotile Coccoid and Colonial Green Algae. Freshwater Algae of North America, Academic Press.","DOI":"10.1016\/B978-012741550-5\/50023-4"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1038\/nclimate2682","article-title":"Growth responses of a green alga to multiple environmental drivers","volume":"5","author":"Brennan","year":"2015","journal-title":"Nat. Clim. Chang."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Sampathkumar, S.J., and Gothandam, K.M. (2019). Sodium bicarbonate augmentation enhances lutein biosynthesis in green microalgae Chlorella pyrenoidosa. Biocatal. Agric. Biotechnol., 22.","DOI":"10.1016\/j.bcab.2019.101406"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.1039\/C8RA09538H","article-title":"Developing a CO2 bicarbonation absorber for promoting microalgal growth rates with an improved photosynthesis pathway","volume":"9","author":"Guo","year":"2019","journal-title":"RSC Adv."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"170325","DOI":"10.1016\/j.scitotenv.2024.170325","article-title":"Carbon capture and utilization by algae with high concentration CO2 or bicarbonate as carbon source","volume":"918","author":"Yang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.bej.2013.11.007","article-title":"Effects of dissolved inorganic carbon and mixing on autotrophic growth of Chlorella vulgaris","volume":"82","author":"Kim","year":"2014","journal-title":"Biochem. Eng. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1080\/09670262.2019.1660808","article-title":"Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation","volume":"55","author":"Li","year":"2020","journal-title":"Eur. J. Phycol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.egypro.2018.10.079","article-title":"Biochemical characterization of Phaeodactylum tricornutum for microalgae-based biorefinery","volume":"153","author":"Agurto","year":"2018","journal-title":"Energy Procedia"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1263\/jbb.101.87","article-title":"Commercial applications of microalgae","volume":"101","author":"Spolaore","year":"2006","journal-title":"J. Biosci. Bioeng."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.biortech.2018.04.092","article-title":"Lipid accumulation of Chlorella pyrenoidosa under mixotrophic cultivation using acetate and ammonium","volume":"262","author":"Liu","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Bellido-Pedraza, C.M., Torres, M.J., and Llamas, A. (2024). The microalgae Chlamydomonas for bioremediation and bioproduct production. Cells, 13.","DOI":"10.20944\/preprints202406.0002.v1"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12934-017-0633-9","article-title":"Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides","volume":"16","author":"Ren","year":"2017","journal-title":"Microb. Cell Factories"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Ruiz, M., Mart\u00ednez-Gonz\u00e1lez, C.A., Kim, D.H., Santiesteban-Romero, B., Reyes-Pardo, H., Villase\u00f1or-Zepeda, K.R., Mel\u00e9ndez-S\u00e1nchez, E.R., Ram\u00edrez-Gamboa, D., D\u00edaz-Zamorano, A.L., and Sosa-Hern\u00e1ndez, J.E. (2022). Microalgae Bioactive Compounds to Topical Applications Products-A Review. Molecules, 27.","DOI":"10.3390\/molecules27113512"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ranjith Kumar, R., Hanumantha Rao, P., and Arumugam, M. (2015). Lipid Extraction Methods from Microalgae: A Comprehensive Review. Front. Energy Res., 2.","DOI":"10.3389\/fenrg.2014.00061"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Wang, Q., Oshita, K., and Takaoka, M. (2021). Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether. Biotechnol. Biofuels, 14.","DOI":"10.1186\/s13068-020-01871-0"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"113282","DOI":"10.1016\/j.foodres.2023.113282","article-title":"Microalgae biomass as an alternative source of biocompounds: New insights and future perspectives of extraction methodologies","volume":"173","author":"Sousa","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Sivaramakrishnan, R., and Incharoensakdi, A. (2019). Low power ultrasound treatment for the enhanced production of microalgae biomass and lipid content. Biocatal. Agric. Biotechnol., 20.","DOI":"10.1016\/j.bcab.2019.101230"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.algal.2018.09.004","article-title":"The effect of high-intensity ultrasound on cell disruption and lipid extraction from high-solids viscous slurries of Nannochloropsis sp. biomass","volume":"35","author":"Yao","year":"2018","journal-title":"Algal Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.biortech.2014.07.089","article-title":"Biodiesel from wet microalgae: Extraction with hexane after the microwave-assisted transesterification of lipids","volume":"170","author":"Cheng","year":"2014","journal-title":"Bioresour. Technol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.microc.2019.01.073","article-title":"Experimental mixture design solvent effects on pigment extraction and antioxidant activity from Coffea arabica L. leaves","volume":"146","author":"Marcheafave","year":"2019","journal-title":"Microchem. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.biortech.2016.07.011","article-title":"Detergent assisted lipid extraction from wet yeast biomass for biodiesel: A response surface methodology approach","volume":"218","author":"Yellapu","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.renene.2018.06.066","article-title":"Oleaginous yeast biomass flocculation using bioflocculant produced in wastewater sludge and transesterification using petroleum diesel as a co-solvent","volume":"131","author":"Yellapu","year":"2019","journal-title":"Renew. Energy"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.bcab.2017.10.004","article-title":"Effect of inducing agents on growth and astaxanthin production in Haematococcus pluvialis: Organic and inorganic","volume":"12","author":"Parakulsuksatid","year":"2017","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.rser.2009.07.020","article-title":"Microalgae for biodiesel production and other applications: A review","volume":"14","author":"Mata","year":"2010","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Santoro, I., Nardi, M., Benincasa, C., Costanzo, P., Giordano, G., Procopio, A., and Sindona, G. (2019). Sustainable and Selective Extraction of Lipids and Bioactive Compounds from Microalgae. Molecules, 24.","DOI":"10.20944\/preprints201911.0080.v1"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Martins, R., Barbosa, A., Advinha, B., Sales, H., Pontes, R., and Nunes, J. (2023). Green Extraction Techniques of Bioactive Compounds: A State-of-the-Art Review. Processes, 11.","DOI":"10.3390\/pr11082255"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"100060","DOI":"10.1016\/j.grets.2023.100060","article-title":"Microalgae-based biodiesel production and its challenges and future opportunities: A review","volume":"2","author":"Gaurav","year":"2024","journal-title":"Green Technol. 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