{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T05:49:27Z","timestamp":1761976167929,"version":"build-2065373602"},"reference-count":90,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Information and Technology of Hungary","award":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"],"award-info":[{"award-number":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"]}]},{"name":"Hungarian Scientific Research Fund (OTKA)","award":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"],"award-info":[{"award-number":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"]}]},{"name":"Medical School, University of Pecs","award":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"],"award-info":[{"award-number":["2021 TKP2021-EGA-37","K 132596 K_19","015_2024_PTE_RK\/44"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Increases in flow elicit dilations in the basilar artery (BA) supplied by the posterior cerebral circulation (PCC), and ensuring efficient blood supply to the circle of Willis in which blood flow and pressure can distribute and equalize, and thus provide the appropriate supply for the daughter branches to reach certain brain areas. In contrast, increases in flow elicit constrictions in the middle cerebral artery (MCA), supplied by the anterior cerebral circulation (ACC) and regulating the blood pressure and flow in distal cerebral circulation. Mediators of flow-dependent responses include arachidonic acid (AA) metabolites and nitric oxide (NO). We hypothesized that mediators of flow-dependent responses are differentially expressed in cerebral arteries of the PCC (CAPCC) and ACC (CAACC). The expressions of key enzymes of the AA pathway\u2014cyclooxygenases (COX1\/COX2), cytochrome P450 hydroxylases (Cyp450), thromboxane synthase (TXAS), thromboxane A2 (TP) receptor, prostacyclin synthase (PGIS), prostacyclin (IP) receptor (IP); neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS)\u2014in the BA and MCA from rats (n = 20) were determined by western blotting. Transcriptome analysis in CAPCC and CAACC from rats (n = 25) was assessed by RNA sequencing. In BA compared to MCA, COX1\/2 and Cyp450 protein expressions were lower, PGIS was higher, TXAS and nNOS\/eNOS were similar, TP receptors were lower, and IP receptors were higher. Gene expressions of vasodilator canonical pathways were higher in CAPCC; vasoconstriction canonical pathways were higher in CAACC. Mediators of flow-dependent vasomotor signaling are differentially expressed in cerebral arteries of the posterior and anterior circulation, corresponding to their vasomotor function.<\/jats:p>","DOI":"10.3390\/life15060856","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T04:49:52Z","timestamp":1748234992000},"page":"856","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Differential Gene and Protein Expressions Responsible for Vasomotor Signaling Provide Mechanistic Bases for the Opposite Flow-Induced Responses of Pre- and Post-Circle of Willis Arteries"],"prefix":"10.3390","volume":"15","author":[{"given":"Zoltan","family":"Nemeth","sequence":"first","affiliation":[{"name":"HUN-REN-SE, Cerebrovascular and Neurocognitive Disease Research Group, Institute of Translational Medicine, Semmelweis University, 1085 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2327-2837","authenticated-orcid":false,"given":"Krisztian","family":"Eros","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Medical School, University of Pecs, 7624 Pecs, Hungary"},{"name":"Szentagothai Research Centre, University of Pecs, 7624 Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9124-4788","authenticated-orcid":false,"given":"Gyongyi","family":"Munkacsy","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Semmelweis University, 1085 Budapest, Hungary"},{"name":"Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3256-8701","authenticated-orcid":false,"given":"Akos","family":"Koller","sequence":"additional","affiliation":[{"name":"HUN-REN-SE, Cerebrovascular and Neurocognitive Disease Research Group, Institute of Translational Medicine, Semmelweis University, 1085 Budapest, Hungary"},{"name":"HUN-REN-SE, Cerebrovascular and Neurocognitive Disease Research Group, Department of Morphology and Physiology, Institute of Translational Medicine, Semmelweis University, 1085 Budapest, Hungary"},{"name":"Research Center for Sport Physiology, Hungarian University of Sports Science, 1123 Budapest, Hungary"},{"name":"Department of Physiology, New York Medical College, Valhalla, NY 10595, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,26]]},"reference":[{"key":"ref_1","unstructured":"Standring, S., and Gray, H. 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