{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:23:43Z","timestamp":1760059423123,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Dysbiosis, or an altered microbiota composition, has been implicated in chronic endometrial inflammation and recurrent implantation failure. Despite growing research on the relationship between the genital microbiome and reproductive health, few studies have examined its role in ectopic pregnancy. Therefore, our study focuses on the microbiota of the cervical canal in women diagnosed with an ectopic pregnancy. Material and methods: The study group consisted of nine women of a reproductive age who were hospitalized at the Department of Maternal and Child Health, Gynecology and Obstetrics, Clinical Hospital of the University of Pozna\u0144, between February and September 2023. In nine patients, an ectopic pregnancy was diagnosed based on a transvaginal ultrasound examination. The swabs were collected for quantitative microbiological culture (using Amies transport medium). The microbiological analyses involved quantitative culture on selected selective and differential media, following the Standard Operating Procedure developed by the Institute of Microecology. Results: A reduced Lactobacillus spp. count (\u22645 \u00d7 107 CFU\/mL) was observed in 78% of the patients participating in the study, including those that produce H2O2, i.e., with strong protective properties for the environment of the female reproductive tract. The molecular analyses revealed Ureaplasma spp. (U. parvum and U. urealyticum) in 33% of the samples (three patients). However, Chlamydia trachomatis and Mycoplasma genitalium were not detected in any of the analyzed samples. Conclusions: The ease of obtaining material and the minimally invasive nature of lower reproductive tract examinations may allow for the evaluation of microbiota imbalances, helping to identify individuals at an increased risk of reproductive complications.<\/jats:p>","DOI":"10.3390\/life15060949","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T03:53:12Z","timestamp":1749786792000},"page":"949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbiota of Cervical Canal in Nine Patients Diagnosed with Ectopic Pregnancy: Case Series"],"prefix":"10.3390","volume":"15","author":[{"given":"Kinga","family":"Bednarek","sequence":"first","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9336-7060","authenticated-orcid":false,"given":"Katarzyna","family":"Wszo\u0142ek","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Szewc","sequence":"additional","affiliation":[{"name":"Institute of Microecology, 60-129 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miros\u0142awa","family":"Ga\u0142\u0119cka","sequence":"additional","affiliation":[{"name":"Institute of Microecology, 60-129 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Mruczy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3791-0524","authenticated-orcid":false,"given":"Alan","family":"Bruszewski","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2619-0466","authenticated-orcid":false,"given":"Marcin","family":"Wierzchowski","sequence":"additional","affiliation":[{"name":"Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Wilczak","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2299-8353","authenticated-orcid":false,"given":"Karolina","family":"Chmaj-Wierzchowska","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health and Minimally Invasive Surgery, Poznan University of Medical Sciences, 60-535 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.ejogrb.2010.08.016","article-title":"Risk factors for rupture in tubal ectopic pregnancy: Definition of the clinical findings","volume":"154","author":"Goksedef","year":"2011","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_2","unstructured":"Br\u0119borowicz, G. (2018). Ci\u0105\u017ca ektopowa, W. Ci\u0105\u017ca Ektopowa. W Po\u0142o\u017cnictwo i Ginekologia, Wydawnictwo Lekarskie PZWL. [2nd ed.]."},{"key":"ref_3","first-page":"61","article-title":"Ci\u0105\u017c\u0105 ektopowa","volume":"1","author":"Jakiel","year":"2006","journal-title":"Przegl\u0105d Menopauzalny"},{"key":"ref_4","first-page":"16","article-title":"Leczenie zachowawcze ci\u0105\u017cy ektopowej","volume":"2","author":"Wydra","year":"2017","journal-title":"Gin. Perinat. Prakt."},{"key":"ref_5","first-page":"431","article-title":"Diagnosis and treatment of ectopic pregnancy","volume":"5","author":"Sobstyl","year":"2012","journal-title":"Przegl\u0105d Menopauzalny"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ajog.2019.01.002","article-title":"Two-dose versus single-dose methotrexate for treatment of ectopic pregnancy: A meta-analysis","volume":"221","author":"Cooney","year":"2019","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4827353","DOI":"10.1155\/2018\/4827353","article-title":"Association Between Prior Chlamydia trachomatis Infection and Ectopic Pregnancy at a Tertiary Care Hospital in South Western Uganda","volume":"2018","author":"Mpiima","year":"2018","journal-title":"Obstet. Gynecol. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e18489","DOI":"10.1097\/MD.0000000000018489","article-title":"Relation of Chlamydia trachomatis Infections to Ectopic Pregnancy: A Meta-Analysis and Systematic Review","volume":"99","author":"Xia","year":"2020","journal-title":"Medicine"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Thirunavuk Arasoo, V.J., Masalamani, M., Ramadas, A., Dominic, N.A., Liew, D.D., Sia, R.W.J., Wanigaratne, A., Weerawarna, K., Wong, W.L.L., and Jeganathan, R. (2022). Association Between Chlamydial Infection With Ectopic and Full-Term Pregnancies: A Case-Control Study. Trop. Med. Infect. Dis., 7.","DOI":"10.3390\/tropicalmed7100285"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.ajog.2018.11.1089","article-title":"Ecological dynamics of the vaginal microbiome in relation to health and disease","volume":"220","author":"Greenbaum","year":"2019","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Petrova, M.I., Lievens, E., Malik, S., Imholz, N., and Lebeer, S. (2015). Lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health. Front. Physiol., 6.","DOI":"10.3389\/fphys.2015.00081"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.tim.2017.07.008","article-title":"Women and Their Microbes: The Unexpected Friendship","volume":"26","author":"Younes","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/1040841X.2017.1306688","article-title":"Defence factors of vaginal lactobacilli","volume":"44","author":"Kovachev","year":"2018","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"230","DOI":"10.26444\/monz\/124891","article-title":"Wp\u0142yw mikrobioty macicy na zdrowie kobiety i jej potomstwa","volume":"26","author":"Macura","year":"2020","journal-title":"Med. Og\u00f3lna Nauk. Zdrowiu"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1126\/science.1058040","article-title":"The sequence of the human genome","volume":"291","author":"Venter","year":"2001","journal-title":"Science"},{"key":"ref_16","first-page":"26","article-title":"Ocena mikroflory bakteryjnej szyjki macicy u kobiet w ci\u0105\u017cy","volume":"5","author":"Milewska","year":"2012","journal-title":"Perinatol. Neonatol. Ginekol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/humupd\/dmy012","article-title":"How uterine microbiota might be responsible for receptive, fertile endometrium","volume":"24","author":"Benner","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0165-0378(02)00019-0","article-title":"The Innate and Early Immune Response to Pathogen Challenge in the Female Genital Tract and the Pivotal Role of Epithelial Cells","volume":"57","author":"Quayle","year":"2002","journal-title":"J. Reprod. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Altm\u00e4e, S. (2018). Commentary: Uterine microbiota: Residents, tourists, or invaders?. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01874"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Liu, Y., Zhang, Y., Yang, D., Xu, C., Huang, Y., Qing, Q., Li, D., Liao, J., Ding, Y., and Zhou, J. (2019). Chlamydia trachomatis and mycoplasma infections in tubal pregnancy. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-52193-7"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Aagaard, K., Riehle, K., Ma, J., Segata, N., Mistretta, T.A., Coarfa, C., Raza, S., Rosenbaum, S., Van den Veyver, I., and Milosavljevic, A. (2012). A metagenomic approach to characterization of the vaginal microbiome signature in pregnancy. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036466"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/s41591-019-0465-8","article-title":"Racioethnic diversity in the dynamics of the vaginal microbiome during pregnancy","volume":"25","author":"Serrano","year":"2019","journal-title":"Nat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ruan, X.F., Zhang, Y.X., Chen, S., Liu, X.R., Zhu, F.F., Huang, Y.X., Liu, X.J., Luo, S.P., Deng, G.P., and Gao, J. (2021). Non-Lactobacillus-Dominated Vaginal Microbiota Is Associated With a Tubal Pregnancy in Symptomatic Chinese Women in the Early Stage of Pregnancy: A Nested Case-Control Study. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.659505"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Teh, H.E., Pung, C.K., Arasoo, V.J.T., and Yap, P.S.X. (2024). A Landscape View of the Female Genital Tract Microbiome in Healthy Controls and Women With Reproductive Health Conditions Associated With Ectopic Pregnancy. Br. J. Biomed. Sci., 80.","DOI":"10.3389\/bjbs.2023.12098"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Gerede, A., Nikolettos, K., Vavoulidis, E., Margioula-Siarkou, C., Petousis, S., Giourga, M., Fotinopoulos, P., Salagianni, M., Stavros, S., and Dinas, K. (2024). Vaginal Microbiome and Pregnancy Complications: A Review. J. Clin. Med., 13.","DOI":"10.20944\/preprints202406.0233.v1"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chen, W., Wei, K., He, X., Wei, J., Yang, L., Li, L., Chen, T., and Tan, B. (2021). Identification of uterine microbiota in infertile women receiving in vitro fertilization with and without chronic endometritis. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.693267"},{"key":"ref_27","first-page":"1069","article-title":"Results of in vitro fertilization after diagnosis and treatment of chronic endometritis","volume":"27","author":"Vaduva","year":"2023","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.fertnstert.2015.06.044","article-title":"Pregnancy outcomes in women with chronic endometritis and recurrent pregnancy loss","volume":"104","author":"McQueen","year":"2015","journal-title":"Fertil. Steril."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/6\/949\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:51:05Z","timestamp":1760032265000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/6\/949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,12]]},"references-count":28,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["life15060949"],"URL":"https:\/\/doi.org\/10.3390\/life15060949","relation":{},"ISSN":["2075-1729"],"issn-type":[{"type":"electronic","value":"2075-1729"}],"subject":[],"published":{"date-parts":[[2025,6,12]]}}}