{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:07:18Z","timestamp":1781068038098,"version":"3.54.1"},"reference-count":45,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372099"],"award-info":[{"award-number":["32372099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LZ25C130011"],"award-info":[{"award-number":["LZ25C130011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LQ24C130007"],"award-info":[{"award-number":["LQ24C130007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2024LYKF06"],"award-info":[{"award-number":["2024LYKF06"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["32372099"],"award-info":[{"award-number":["32372099"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LZ25C130011"],"award-info":[{"award-number":["LZ25C130011"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LQ24C130007"],"award-info":[{"award-number":["LQ24C130007"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["2024LYKF06"],"award-info":[{"award-number":["2024LYKF06"]}]},{"name":"Environment-friendly Crop Germplasm Innovational and Genetic Improvement Key Laboratory of Sichuan Province","award":["32372099"],"award-info":[{"award-number":["32372099"]}]},{"name":"Environment-friendly Crop Germplasm Innovational and Genetic Improvement Key Laboratory of Sichuan Province","award":["LZ25C130011"],"award-info":[{"award-number":["LZ25C130011"]}]},{"name":"Environment-friendly Crop Germplasm Innovational and Genetic Improvement Key Laboratory of Sichuan Province","award":["LQ24C130007"],"award-info":[{"award-number":["LQ24C130007"]}]},{"name":"Environment-friendly Crop Germplasm Innovational and Genetic Improvement Key Laboratory of Sichuan Province","award":["2024LYKF06"],"award-info":[{"award-number":["2024LYKF06"]}]},{"name":"Agricultural Science and Technology Innovation Program (ASTIP)","award":["32372099"],"award-info":[{"award-number":["32372099"]}]},{"name":"Agricultural Science and Technology Innovation Program (ASTIP)","award":["LZ25C130011"],"award-info":[{"award-number":["LZ25C130011"]}]},{"name":"Agricultural Science and Technology Innovation Program (ASTIP)","award":["LQ24C130007"],"award-info":[{"award-number":["LQ24C130007"]}]},{"name":"Agricultural Science and Technology Innovation Program (ASTIP)","award":["2024LYKF06"],"award-info":[{"award-number":["2024LYKF06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Starch is the primary component of the endosperm and plays a crucial role in rice quality. Although the enzymes involved in starch synthesis have been extensively studied, the transcription factors that regulate these enzymes remain largely unknown. Here, we identified a MYB family transcription factor, OsMYBR1, that regulates starch biosynthesis in rice. OsMYBR1 is highly expressed during endosperm development. Mutations of OsMYBR1 result in reduced grain thickness and a decrease in 1000-grain weight. The endosperm of osmybr1 mutants exhibit rounded and loosely packed starch granules, decreased amylose content, altered fine structure of amylopectin, and modified physicochemical properties. The analysis of RT-qPCR showed that the expression of several starch-synthesis enzyme-coding genes (SSEGs), including OsGBSS\u2160, OsAGPL1, OsAGPL2, OsBE\u2161b, OsISA1, PHOL, and OsSS\u2162a, is altered in osmybr1 mutants. Further experiments indicated that OsMYBR1 directly binds to the promoters of OsGBSS\u2160, OsAGPL1, OsAGPL2, OsISA1, OsBE\u2161b, and PHOL, resulting in an increase in the expression of OsGBSS\u2160 but a decrease in the expression of OsAGPL2, OsISA1, and OsSS\u2162a. In contrast, OsMYBR1-overexpressing endosperm appears normal, with starch granule morphology, increased amylopectin content, and improved alkali spreading value, indicating enhanced rice eating and cooking quality (ECQ). These findings suggest that the overexpression of OsMYBR1 could be a promising strategy for improving rice ECQ.<\/jats:p>","DOI":"10.3390\/life15060962","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T04:20:23Z","timestamp":1750134023000},"page":"962","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["OsMYBR1, a 1R-MYB Family Transcription Factor Regulates Starch Biosynthesis in Rice Endosperm"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3847-6931","authenticated-orcid":false,"given":"Kunyong","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"},{"name":"Environment-Friendly Crop Germplasm Innovational and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiai","family":"Jiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheyan","family":"Ruan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqing","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8568-5621","authenticated-orcid":false,"given":"Peisong","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1190-5222","authenticated-orcid":false,"given":"Xiangjin","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1104\/pp.20.00984","article-title":"The NAC transcription factors OsNAC20 and OsNAC26 regulate starch and storage protein synthesis","volume":"184","author":"Wang","year":"2020","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev-arplant-042809-112301","article-title":"Starch: Its metabolism, evolution, and biotechnological modification in plants","volume":"61","author":"Zeeman","year":"2010","journal-title":"Annu. 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