{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:53:47Z","timestamp":1789581227515,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T00:00:00Z","timestamp":1752278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Acinetobacter baumannii is listed by the World Health Organization as an emerging bacterial priority pathogen, the prevalence and multidrug resistance of which have been increasing. This functional genomics study aimed to understand the drug-resistance mechanisms of an extensively drug-resistant (XDR) A. baumannii strain (IRMCBCU95U) isolated from a transtracheal aspirate sample from a female patient with end-stage renal disease in Saudi Arabia. The whole genome of IRMCBCU95U (4.3 Mbp) was sequenced using Oxford Nanopore long-read sequencing to identify and compare the antibiotic-resistance profile and genomic features of A. baumannii IRMCBCU95U. The antibiogram of A. baumannii IRMCBCU95U revealed resistance to multiple antibiotics, including cefepime, ceftazidime, ciprofloxacin, imipenem, meropenem and piperacillin\/tazobactam. A comparative genomic analysis between IRMCBCU95U and A. baumannii K09-14 and ATCC 19606 identified significant genetic heterogeneity and mosaicism among the strains. This analysis also demonstrated the hybrid nature of the genome of IRMCBCU95U and indicates that horizontal gene transfer may have occurred between these strains. The IRMCBCU95U genome has a diverse range of genes associated with antimicrobial resistance and mobile genetic elements (ISAba1 and IS26) associated with the spread of multidrug resistance. The presence of virulence-associated genes that are linked to iron acquisition, motility and transcriptional regulation confirmed that IRMCBCU95U is a priority human pathogen. The plasmid fragment IncFIB(pNDM-Mar) observed in the strain is homologous to the plasmid in Klebsiella pneumoniae (439 bp; similarity: 99.09%), which supports its antimicrobial resistance. From these observations, it can be concluded that the clinical A. baumannii IRMCBCU95U isolate is an emerging extensively drug-resistant human pathogen with a novel combination of resistance genes and a plasmid fragment. The complex resistome of IRMCBCU95U highlights the urgent need for genomic surveillance in hospital settings in Saudi Arabia to fight against the spread of extensively drug-resistant A. baumannii.<\/jats:p>","DOI":"10.3390\/life15071094","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T07:54:23Z","timestamp":1752479663000},"page":"1094","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hybrid Genome and Clinical Impact of Emerging Extensively Drug-Resistant Priority Bacterial Pathogen Acinetobacter baumannii in Saudi Arabia"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7199-1540","authenticated-orcid":false,"given":"J. Francis","family":"Borgio","sequence":"first","affiliation":[{"name":"Department of Genetic Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia"},{"name":"Department of Epidemic Diseases Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shi, J., Cheng, J., Liu, S., Zhu, Y., and Zhu, M. (2024). Acinetobacter baumannii: An Evolving and Cunning Opponent. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1332108"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.4161\/viru.19700","article-title":"Acinetobacter baumannii: An Emerging Opportunistic Pathogen","volume":"3","author":"Howard","year":"2012","journal-title":"Virulence"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/nrmicro.2017.148","article-title":"Uncovering the Mechanisms of Acinetobacter baumannii Virulence","volume":"16","author":"Harding","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Arshad, N., Azzam, W., Zilberberg, M.D., and Shorr, A.F. (2025). Acinetobacter baumannii Complex Infections: New Treatment Options in the Antibiotic Pipeline. Microorganisms, 13.","DOI":"10.3390\/microorganisms13020356"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"dlaf039","DOI":"10.1093\/jacamr\/dlaf039","article-title":"Iron-Dependent Mechanisms in Acinetobacter baumannii: Pathogenicity and Resistance","volume":"7","author":"Zhang","year":"2025","journal-title":"JAC Antimicrob. Resist."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1086\/590568","article-title":"Acinetobacter baumannii Skin and Soft-Tissue Infection Associated with War Trauma","volume":"47","author":"Sebeny","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/cc9997","article-title":"New Treatment Options against Gram-Negative Organisms","volume":"15","author":"Bassetti","year":"2011","journal-title":"Crit. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1086\/533452","article-title":"Federal Funding for the Study of Antimicrobial Resistance in Nosocomial Pathogens: No ESKAPE","volume":"197","author":"Rice","year":"2008","journal-title":"J. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Fournier, P.-E., Vallenet, D., Barbe, V., Audic, S., Ogata, H., Poirel, L., Richet, H., Robert, C., Mangenot, S., and Abergel, C. (2006). Comparative Genomics of Multidrug Resistance in Acinetobacter baumannii. PLoS Genet., 2.","DOI":"10.1371\/journal.pgen.0020007"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s40265-024-02104-6","article-title":"Treatment Approaches for Carbapenem-Resistant Acinetobacter baumannii Infections","volume":"85","author":"Iovleva","year":"2025","journal-title":"Drugs"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13073-016-0279-y","article-title":"Genome Dynamics of Multidrug-Resistant Acinetobacter baumannii during Infection and Treatment","volume":"8","author":"Wright","year":"2016","journal-title":"Genome Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Chan, A.P., Sutton, G., DePew, J., Krishnakumar, R., Choi, Y., Huang, X.-Z., Beck, E., Harkins, D.M., Kim, M., and Lesho, E.P. (2015). A Novel Method of Consensus Pan-Chromosome Assembly and Large-Scale Comparative Analysis Reveal the Highly Flexible Pan-Genome of Acinetobacter baumannii. Genome Biol., 16.","DOI":"10.1186\/s13059-015-0701-6"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Borgio, J.F., Rasdan, A.S., Sonbol, B., Alhamid, G., Almandil, N.B., and AbdulAzeez, S. (2021). Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula. Biology, 10.","DOI":"10.3390\/biology10111144"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alamri, A.M., Alsultan, A.A., Ansari, M.A., and Alnimr, A.M. (2020). Biofilm-Formation in Clonally Unrelated Multidrug-Resistant Acinetobacter baumannii Isolates. Pathogens, 9.","DOI":"10.3390\/pathogens9080630"},{"key":"ref_15","first-page":"646195","article-title":"Acinetobacter baumannii Septicemia in a Recipient of an Allogeneic Hematopoietic Stem Cell Transplantation","volume":"2012","author":"Abdalhamid","year":"2012","journal-title":"Case Rep. Transpl."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.22207\/JPAM.12.4.31","article-title":"Genetic Relationship of Multi-Resistant Acinetobacter baumannii Isolates in Kingdom of Saudi Arabia","volume":"12","author":"Aljindan","year":"2018","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9599","DOI":"10.1073\/pnas.1523116113","article-title":"Acinetobacter baumannii Phenylacetic Acid Metabolism Influences Infection Outcome through a Direct Effect on Neutrophil Chemotaxis","volume":"113","author":"Bhuiyan","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.tim.2012.12.002","article-title":"Emerging Therapies for Multidrug Resistant Acinetobacter baumannii","volume":"21","author":"Pulido","year":"2013","journal-title":"Trends Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e01076-14","DOI":"10.1128\/mBio.01076-14","article-title":"AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments","volume":"5","author":"Jacobs","year":"2014","journal-title":"mBio"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102463","DOI":"10.1016\/j.jiph.2024.05.051","article-title":"Phenomics and Genomic Features of Enterococcus Avium IRMC1622a Isolated from a Clinical Sample of Hospitalized Patient","volume":"17","author":"AlJindan","year":"2024","journal-title":"J. Infect. Public Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e13056","DOI":"10.7717\/peerj.13056","article-title":"NanoForms: An Integrated Server for Processing, Analysis and Assembly of Raw Sequencing Data of Microbial Genomes, from Oxford Nanopore Technology","volume":"10","author":"Czmil","year":"2022","journal-title":"PeerJ"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","article-title":"NanoPack: Visualizing and Processing Long-Read Sequencing Data","volume":"34","author":"Schultz","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"i142","DOI":"10.1093\/bioinformatics\/bty266","article-title":"Versatile Genome Assembly Evaluation with QUAST-LG","volume":"34","author":"Mikheenko","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"E8396","DOI":"10.1073\/pnas.1604560113","article-title":"Assembly of Long Error-Prone Reads Using de Bruijn Graphs","volume":"113","author":"Lin","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"D535","DOI":"10.1093\/nar\/gkw1017","article-title":"Improvements to PATRIC, the All-Bacterial Bioinformatics Database and Analysis Resource Center","volume":"45","author":"Wattam","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"giaa105","DOI":"10.1093\/gigascience\/giaa105","article-title":"NanoGalaxy: Nanopore Long-Read Sequencing Data Analysis in Galaxy","volume":"9","author":"Miladi","year":"2020","journal-title":"Gigascience"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"AlJindan, R., AlEraky, D.M., Farhat, M., Almandil, N.B., AbdulAzeez, S., and Borgio, J.F. (2023). Genomic Insights into Virulence Factors and Multi-Drug Resistance in Clostridium Perfringens IRMC2505A. Toxins, 15.","DOI":"10.3390\/toxins15060359"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4261","DOI":"10.1016\/j.csbj.2023.08.031","article-title":"Mining the Nanotube-Forming Bacillus Amyloliquefaciens MR14M3 Genome for Determining Anti-Candida Auris and Anti-Candida Albicans Potential by Pathogenicity and Comparative Genomics Analysis","volume":"21","author":"Borgio","year":"2023","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D431","DOI":"10.1093\/nar\/gkh081","article-title":"BRENDA, the Enzyme Database: Updates and Major New Developments","volume":"32","author":"Schomburg","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a Reference Resource for Gene and Protein Annotation","volume":"44","author":"Kanehisa","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the Unification of Biology. The Gene Ontology Consortium","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Davis, J.J., Gerdes, S., Olsen, G.J., Olson, R., Pusch, G.D., Shukla, M., Vonstein, V., Wattam, A.R., and Yoo, H. (2016). PATtyFams: Protein Families for the Microbial Genomes in the PATRIC Database. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00118"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5691","DOI":"10.1093\/nar\/gki866","article-title":"The Subsystems Approach to Genome Annotation and Its Use in the Project to Annotate 1000 Genomes","volume":"33","author":"Overbeek","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/AAC.00419-13","article-title":"The Comprehensive Antibiotic Resistance Database","volume":"57","author":"McArthur","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"D1091","DOI":"10.1093\/nar\/gkt1068","article-title":"DrugBank 4.0: Shedding New Light on Drug Metabolism","volume":"42","author":"Law","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"D787","DOI":"10.1093\/nar\/gkp1014","article-title":"Update of TTD: Therapeutic Target Database","volume":"38","author":"Zhu","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: Hierarchical and Refined Dataset for Big Data Analysis--10 Years On","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/bioinformatics\/btu631","article-title":"Curation, Integration and Visualization of Bacterial Virulence Factors in PATRIC","volume":"31","author":"Mao","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"D372","DOI":"10.1093\/nar\/gkv1103","article-title":"The Transporter Classification Database (TCDB): Recent Advances","volume":"44","author":"Saier","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In Silico Detection and Typing of Plasmids Using PlasmidFinder and Plasmid Multilocus Sequence Typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ondov, B.D., Treangen, T.J., Melsted, P., Mallonee, A.B., Bergman, N.H., Koren, S., and Phillippy, A.M. (2016). Mash: Fast Genome and Metagenome Distance Estimation Using MinHash. Genome Biol., 17.","DOI":"10.1186\/s13059-016-0997-x"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple Sequence Alignment with High Accuracy and High Throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML Version 8: A Tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1080\/10635150802429642","article-title":"A Rapid Bootstrap Algorithm for the RAxML Web Servers","volume":"57","author":"Stamatakis","year":"2008","journal-title":"Syst. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Clausen, P.T.L.C., Aarestrup, F.M., and Lund, O. (2018). Rapid and Precise Alignment of Raw Reads against Redundant Databases with KMA. BMC Bioinform., 19.","DOI":"10.1186\/s12859-018-2336-6"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"D871","DOI":"10.1093\/nar\/gkae968","article-title":"VFDB 2025: An Integrated Resource for Exploring Anti-Virulence Compounds","volume":"53","author":"Zhou","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1093\/jac\/dkz092","article-title":"LRE-Finder, a Web Tool for Detection of the 23S rRNA Mutations and the optrA, Cfr, Cfr(B) and poxtA Genes Encoding Linezolid Resistance in Enterococci from Whole-Genome Sequences","volume":"74","author":"Hasman","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Cosentino, S., Voldby Larsen, M., M\u00f8ller Aarestrup, F., and Lund, O. (2013). PathogenFinder--Distinguishing Friend from Foe Using Bacterial Whole Genome Sequence Data. PLoS ONE, 8.","DOI":"10.1371\/annotation\/b84e1af7-c127-45c3-be22-76abd977600f"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/jac\/dkaa390","article-title":"Detection of Mobile Genetic Elements Associated with Antibiotic Resistance in Salmonella Enterica Using a Newly Developed Web Tool: MobileElementFinder","volume":"76","author":"Johansson","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jgar.2019.06.021","article-title":"Draft Genome Sequence of a Clinical Acinetobacter baumannii Isolate of New Sequence Type ST1688 from Saudi Arabia","volume":"18","author":"Yasir","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Al-Zahrani, I.A., and Brek, T.M. (2024). Comprehensive Genome Analysis of Colistin-Only-Sensitive KPC-2 and NDM1-1-Coproducing Klebsiella pneumoniae ST11 and Acinetobacter baumannii ST2 From a Critically Ill Patient With COVID-19 in Saudi Arabia: Whole Genome Sequencing (WGS) of K. pneumoniae ST11 and A. baumannii ST2. Int. J. Microbiol., 2024.","DOI":"10.1155\/2024\/9233075"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jgar.2025.03.012","article-title":"Genomic Insights of Predominant International High-Risk Clone ST2 Acinetobacter baumannii Isolates in Saudi Arabia","volume":"42","author":"Brek","year":"2025","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Okdah, L., AlDosary, M.S., AlMazyed, A., Alkhurayb, H.M., Almossallam, M., Al Obaisi, Y.S., Marie, M.A., Abdelrahman, T., and Diene, S.M. (2022). Genomic Characterization of Colistin-Resistant Isolates from the King Fahad Medical City, Kingdom of Saudi Arabia. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111597"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Yasir, M., Subahi, A.M., Shukri, H.A., Bibi, F., Sohrab, S.S., Alawi, M., Sindi, A.A., Jiman-Fatani, A.A., and Azhar, E.I. (2022). Bacterial Community and Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Isolates from the Environment of a Health Care Facility in the Western Region of Saudi Arabia. Pharmaceuticals, 15.","DOI":"10.3390\/ph15050611"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1089\/mdr.2014.0018","article-title":"Characterization of Acinetobacter baumannii Clinical Isolates Carrying Bla(OXA-23) Carbapenemase and 16S rRNA Methylase armA Genes in Yemen","volume":"20","author":"Bakour","year":"2014","journal-title":"Microb. Drug Resist."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.1093\/jac\/dkw184","article-title":"Benchmarking of Methods for Identification of Antimicrobial Resistance Genes in Bacterial Whole Genome Data","volume":"71","author":"Clausen","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Shankar, C., Vasudevan, K., Jacob, J.J., Baker, S., Isaac, B.J., Neeravi, A.R., Sethuvel, D.P.M., George, B., and Veeraraghavan, B. (2022). Hybrid Plasmids Encoding Antimicrobial Resistance and Virulence Traits Among Hypervirulent Klebsiella pneumoniae ST2096 in India. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.875116"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zeng, Z., Lei, L., Li, L., Hua, S., Li, W., Zhang, L., Lin, Q., Zheng, Z., Yang, J., and Dou, X. (2022). In Silico Characterization of blaNDM-Harboring Plasmids in Klebsiella pneumoniae. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1008905"},{"key":"ref_61","first-page":"672","article-title":"NDM-1 (New Delhi Metallo Beta Lactamase-1) Producing Gram-Negative Bacilli: Emergence & Clinical Implications","volume":"140","author":"Fomda","year":"2014","journal-title":"Indian J. Med. Res."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/7\/1094\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:08:58Z","timestamp":1760033338000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/7\/1094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,12]]},"references-count":61,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["life15071094"],"URL":"https:\/\/doi.org\/10.3390\/life15071094","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,12]]}}}