{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:40:08Z","timestamp":1769640008552,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University","award":["Functional Food project MMJW1"],"award-info":[{"award-number":["Functional Food project MMJW1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Due to the stroke-protective effects of dietary fiber and anthocyanin together with the synergistic interaction, we hypothesized that the functional drink containing the anthocyanins and dietary fiber-enriched functional ingredient from banana and germinated black Jasmine rice (BR) should protect against ischemic stroke. BR at doses of 300, 600, and 900 mg\/kg body weight (BW) was orally given to male Wistar rats weighing 290\u2013350 g once daily for 21 days, and they were subjected to ischemic reperfusion injury induced by temporary occlusion of the middle cerebral artery (MCAO\/IR) for 90 min. The treatment was prolonged for 21 days after MCAO\/IR. They were assessed for brain infarction volume, neuron density, Nrf2, MDA, and catalase in the cortex together with serum TNF-\u03b1 and IL-6. Lactobacillus and Bifidobacterium spp. in feces were also assessed. Our results showed that BR improved the increase in brain infarcted volume, MDA, TNF-\u03b1, and IL-6 and the decrease in neuron density, Nrf2, catalase, and both bacteria spp. induced by MCAO\/IR. These data suggest the stroke-protective effect of the novel functional drink, and the action may involve the improvement of Nrf2, oxidative stress, inflammation, and the amount of Lactobacillus and Bifidobacterium spp.<\/jats:p>","DOI":"10.3390\/life15081222","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T09:41:17Z","timestamp":1754300477000},"page":"1222","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neuroprotective Effect Against Ischemic Stroke of the Novel Functional Drink Containing Anthocyanin and Dietary Fiber Enriched-Functional Ingredient from the Mixture of Banana and Germinated Jasmine Rice"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0001-5408","authenticated-orcid":false,"given":"Mubarak","family":"Muhammad","sequence":"first","affiliation":[{"name":"Neuroscience Program, Department of Physiology, Graduate School, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7383-2348","authenticated-orcid":false,"given":"Jintanaporn","family":"Wattanathorn","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wipawee","family":"Thukham-mee","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophida","family":"Phuthong","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7756-1955","authenticated-orcid":false,"given":"Supaporn","family":"Muchimapura","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Institute for High Human Performance and Health Promotion, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"eClinicalMedicine (2023). The rising global burden of stroke. EClinicalMedicine, 59, 102028.","DOI":"10.1016\/j.eclinm.2023.102028"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/17474930211065917","article-title":"World Stroke Organization (WSO): Global Stroke Fact Sheet 2022","volume":"17","author":"Feigin","year":"2022","journal-title":"Int. J. Stroke"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4103\/bc.bc_36_18","article-title":"Treatment for ischemic stroke: From thrombolysis to thrombectomy and remaining challenges","volume":"5","author":"Leng","year":"2019","journal-title":"Brain Circ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1161\/STROKEAHA.114.006288","article-title":"Challenges of acute endovascular stroke trials","volume":"45","author":"Goyal","year":"2014","journal-title":"Stroke"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6057","DOI":"10.1097\/MS9.0000000000001441","article-title":"Stroke and cognitive impairment: Understanding the connection and managing symptoms","volume":"85","author":"Elendu","year":"2023","journal-title":"Ann. Med. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e0230603","DOI":"10.1371\/journal.pone.0230603","article-title":"Agency judgments in post-stroke patients with sensorimotor deficits","volume":"15","author":"Miyawaki","year":"2020","journal-title":"PLoS ONE"},{"key":"ref_7","first-page":"101","article-title":"Quality of Life of Post-stroke Patients","volume":"61","year":"2022","journal-title":"Zdr. Varst."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Morone, G., and Pichiorri, F. (2023). Post-Stroke Rehabilitation: Challenges and New Perspectives. J. Clin. Med., 12.","DOI":"10.3390\/jcm12020550"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"112","DOI":"10.4314\/mmj.v31i2.2","article-title":"The challenges and experiences of stroke patients and their spouses in Blantyre, Malawi","volume":"31","author":"Kalavina","year":"2019","journal-title":"Malawi Med. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000363617","article-title":"Prehospital stroke care: Limitations of current interventions and focus on new developments","volume":"38","author":"Yperzeele","year":"2014","journal-title":"Cerebrovasc. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.reth.2021.09.009","article-title":"Current status of ischemic stroke treatment: From thrombolysis to potential regenerative medicine","volume":"18","author":"Hurd","year":"2021","journal-title":"Regen. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3389\/fnmol.2020.00028","article-title":"Targeting Oxidative Stress and Inflammation to Prevent Ischemia-Reperfusion Injury","volume":"13","author":"Wu","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8418820","DOI":"10.1155\/2022\/8418820","article-title":"The Role of Oxidative Stress in Acute Ischemic Stroke-Related Thrombosis","volume":"2022","author":"Li","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s00281-023-00994-4","article-title":"Thromboinflammatory challenges in stroke pathophysiology","volume":"45","author":"Szepanowski","year":"2023","journal-title":"Semin. Immunopathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.1113\/jphysiol.2011.212886","article-title":"Ischaemic stroke: A thrombo-inflammatory disease?","volume":"589","author":"Nieswandt","year":"2011","journal-title":"J. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/s41538-024-00297-4","article-title":"Implementing a food first strategy can transform preventive healthcare","volume":"8","author":"Yu","year":"2024","journal-title":"NPJ Sci. Food"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/S1474-4422(20)30433-6","article-title":"The role of diet in secondary stroke prevention","volume":"20","author":"English","year":"2021","journal-title":"Lancet Neurol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1161\/STROKEAHA.114.004836","article-title":"Fruits and vegetables consumption and risk of stroke: A meta-analysis of prospective cohort studies","volume":"45","author":"Hu","year":"2014","journal-title":"Stroke"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1212\/01.WNL.0000090458.67821.A3","article-title":"Dietary antioxidants and the risk of ischemic stroke: The Rotterdam Study","volume":"61","author":"Hollander","year":"2003","journal-title":"Neurology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"107144","DOI":"10.1016\/j.jstrokecerebrovasdis.2023.107144","article-title":"The relationship between dietary fibre and stroke: A meta-analysis","volume":"32","author":"Li","year":"2023","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14719\/pst.1817","article-title":"Blackrice: A review from its history to chemicalmakeup to health advantages, nutritionalproperties and dietary uses","volume":"9","author":"Panda","year":"2022","journal-title":"Plant Sci. Today"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100462","DOI":"10.1016\/j.focha.2023.100462","article-title":"Black rice: A comprehensive review on its bioactive compounds, potential health benefits and food applications","volume":"3","author":"Das","year":"2023","journal-title":"Food Chem. Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1007\/s13197-011-0232-4","article-title":"Germinated brown rice as a value added rice product: A review","volume":"48","author":"Patil","year":"2011","journal-title":"J. Food Sci. Technol."},{"key":"ref_24","first-page":"989","article-title":"Banana fruit pulp and peel involved in antianxiety and antidepressant effects while invigorate memory performance in male mice: Possible role of potential antioxidants","volume":"30","author":"Samad","year":"2017","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1007\/s13197-018-03562-z","article-title":"Production, application and health effects of banana pulp and peel flour in the food industry","volume":"56","author":"Birch","year":"2019","journal-title":"J. Food Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e14364","DOI":"10.7717\/peerj.14364","article-title":"Supplementation of banana peel powder for the development of functional broiler nuggets","volume":"10","author":"Akram","year":"2022","journal-title":"PeerJ"},{"key":"ref_27","first-page":"39","article-title":"Banana peel extract has antimicrobial, antioxidant, anti-inflammatory, and wound healing potential\u2014A review","volume":"10","author":"Anandhi","year":"2023","journal-title":"J. Surv. Fish. Sci."},{"key":"ref_28","first-page":"e51260","article-title":"Antioxidant and Anti-inflammatory Properties of the Two Varieties of Musa acuminata: An In Vitro Study","volume":"15","author":"Balajee","year":"2023","journal-title":"Cureus"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1161\/01.STR.31.9.2112","article-title":"Piracetam improves activated blood flow and facilitates rehabilitation of poststroke aphasic patients","volume":"31","author":"Kessler","year":"2000","journal-title":"Stroke"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1097\/00004647-200001000-00007","article-title":"An absolute measurement of brain water content using magnetic resonance imaging in two focal cerebral ischemic rat models","volume":"20","author":"Lin","year":"2000","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5187102","DOI":"10.1155\/2017\/5187102","article-title":"Neuroprotective and Memory-Enhancing Effect of the Combined Extract of Purple Waxy Corn Cob and Pandan in Ovariectomized Rats","volume":"2017","author":"Kirisattayakul","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"9658267","DOI":"10.1155\/2019\/9658267","article-title":"Cerebroprotective Effect against Cerebral Ischemia of the Combined Extract of Oryza sativa and Anethum graveolens in Metabolic Syndrome Rats","volume":"2019","author":"Wattanathorn","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_33","first-page":"505","article-title":"A review of the anatomy of the middle cerebral artery for the era of thrombectomy: A radiologic tool based on CT angiography and perfusion CT","volume":"63","year":"2021","journal-title":"Radiologia"},{"key":"ref_34","unstructured":"Pluta, R. (2025, June 28). The Anatomy of the Hippocampus, Cerebral Ischemia [Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK575732\/."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1080\/14728222.2024.2401518","article-title":"The role of Nrf2 in immune cells and inflammatory autoimmune diseases: A comprehensivereview","volume":"28","author":"Xu","year":"2024","journal-title":"Expert. Opin. Ther. Targets"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"54","DOI":"10.15586\/aei.v51i1.732","article-title":"Nrf2 regulates the expression of NOX1 in TNF-\u03b1-induced A549 cells","volume":"51","author":"Wu","year":"2023","journal-title":"Allergol. Immunopathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11624","DOI":"10.1038\/ncomms11624","article-title":"Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription","volume":"7","author":"Kobayashi","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Almeida-Becerril, T., Rodr\u00edguez-Cruz, M., Villa-Morales, J., S\u00e1nchez-Mendoza, C.R., and Galeazzi-Aguilar, J.E. (2023). Circulating Nrf2, Glutathione, and Malondialdehyde Correlate with Disease Severity in Duchenne Muscular Dystrophy. Antioxidants, 12.","DOI":"10.3390\/antiox12040871"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wang, L., Zhang, X., Xiong, X., Zhu, H., Chen, R., Zhang, S., Chen, G., and Jian, Z. (2022). Nrf2 Regulates Oxidative Stress and Its Role in Cerebral Ischemic Stroke. Antioxidants, 11.","DOI":"10.3390\/antiox11122377"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1590\/S0100-879X2004000400008","article-title":"Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain","volume":"37","author":"Schilichting","year":"2004","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e37682","DOI":"10.1097\/MD.0000000000037682","article-title":"Bifidobacterium bifidum supplementation improves ischemic stroke outcomes in elderly patients: A retrospective study","volume":"103","author":"Xin","year":"2024","journal-title":"Medicine"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1008514","DOI":"10.3389\/fnut.2022.1008514","article-title":"Gut microbiota in ischemic stroke: Where we stand and challenges ahead","volume":"9","author":"Long","year":"2022","journal-title":"Front. Nutr."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/8\/1222\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:21:50Z","timestamp":1760034110000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/8\/1222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,2]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["life15081222"],"URL":"https:\/\/doi.org\/10.3390\/life15081222","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,2]]}}}