{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T05:25:56Z","timestamp":1775625956120,"version":"3.50.1"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Research and Innovation at University of Hafr Al Batin","award":["0119-1446-S"],"award-info":[{"award-number":["0119-1446-S"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>This study presents an integrated approach combining molecular, phytochemical, and biological analyses to characterize a newly discovered Zizania specimen from the northern Nile Delta, Egypt. Genetic fingerprinting using RAPD and ISSR markers revealed 85% band-sharing similarity with Zizania texana (Z. texana), though distinct morphological and genetic traits suggested potential intraspecific variation. Phytochemical profiling identified high concentrations of bioactive compounds, including quercetin (42.1 \u00b5g\/mL), \u03b2-caryophyllene (11.21%), and gallic acid (23.4 \u00b5g\/mL), which are pertinent and correlated with robust biological activities. The ethanolic leaf extract exhibited significant antioxidant capacity (IC50 = 38.6 \u00b5g\/mL in DPPH assay), potent antimicrobial effects against Candida albicans (C. albicans) (IC50 = 4.9 \u00b1 0.6 \u00b5g\/mL), and dose-dependent cytotoxicity against cancer cell lines. MCF-7 has the lowest IC50 (28.3 \u00b1 1.5 \u00b5g\/mL), indicating the highest potency among the tested cell lines. In contrast, HepG2 demonstrates moderate sensitivity (IC50 = 31.4 \u00b1 1.8 \u00b5g\/mL), while A549 shows the highest IC50 value (36.9 \u00b1 2.0 \u00b5g\/mL), indicating greater resistance. These findings underscore the taxonomic novelty of the specimen and its potential as a source of natural antioxidants, antimicrobials, and anticancer agents. The study highlights the importance of interdisciplinary approaches in resolving taxonomic uncertainties and unlocking the medicinal value of understudied aquatic plants.<\/jats:p>","DOI":"10.3390\/life15081240","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T07:49:58Z","timestamp":1754380198000},"page":"1240","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["DNA Fingerprint Profile of Zizania spp. Plant, Monitoring Its Leaves with Screening of Their Biological Activity: Antimicrobial, Antioxidant and Cytotoxicity"],"prefix":"10.3390","volume":"15","author":[{"given":"Latifah A.","family":"Al Shammari","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hafr Al Batin, P.O. 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