{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:39:12Z","timestamp":1760060352120,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Innovation &amp; Research Secretariat (SPI) of Faculty of Medicine National University of Malaysia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Transvaginal ultrasound-guided follicle aspiration remains the gold standard for oocyte retrieval in assisted reproductive technology (ART). This procedure employs either a single-lumen aspiration needle (SLN) or double-lumen aspiration needle (DLN), both of which are effective modalities for oocyte retrieval. The primary objective of this review is to systematically compare the impact and clinical outcomes associated with the use of SLN versus DLN in women undergoing ART. A systematic literature search was conducted across two databases, PubMed and Google Scholar, encompassing publications from their inception until May 2025, and articles published in English. A total of five studies were included in the final analysis. The oocyte yield and the number of MII oocyte did not differ significantly between the groups. Procedural duration was markedly shorter in the SLN group compared to the DLN group. No significant differences were observed in procedure-related complications across groups. Two randomized controlled trials reported comparable fertilization rates and numbers of high-quality embryos between the two needle types. Additionally, clinical pregnancy rates, ongoing pregnancy rates, and live birth rates were similar between the SLN and DLN groups. In conclusion, the utilization ofan SLN for oocyte retrieval demonstrates comparable efficacy to that of a DLN.<\/jats:p>","DOI":"10.3390\/life15091334","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T07:41:45Z","timestamp":1755848505000},"page":"1334","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single or Double-Lumen Aspiration Needle? Revisiting Choices for In Vitro Fertilization or Intracytoplasmic Sperm Injection\u2014A Critical Review"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9225-3521","authenticated-orcid":false,"given":"Gopinath","family":"Muruti","sequence":"first","affiliation":[{"name":"Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2735-3274","authenticated-orcid":false,"given":"Mohd Faizal","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia"},{"name":"Department of Obstetrics & Gynaecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0383-572X","authenticated-orcid":false,"given":"Muhammad Azrai","family":"Abu","sequence":"additional","affiliation":[{"name":"Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia"},{"name":"Department of Obstetrics & Gynaecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurul Ilani Abdul","family":"Latif","sequence":"additional","affiliation":[{"name":"Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia"},{"name":"Department of Obstetrics & Gynaecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Kadir Abdul","family":"Karim","sequence":"additional","affiliation":[{"name":"Advanced Reproductive Centre, Hospital Canselor Tuanku Muhriz, National University of Malaysia, Kuala Lumpur 56000, Malaysia"},{"name":"Department of Obstetrics & Gynaecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1007\/s43032-023-01232-w","article-title":"A Monocentric Randomized Controlled Clinical Trial to Compare Single- and Double-Lumen Needles in Oocyte Retrieval Procedure in Assisted Reproductive Technologies","volume":"9","author":"Ronchetti","year":"2023","journal-title":"Reprod. Sci."},{"key":"ref_2","first-page":"272","article-title":"Evaluation of follicular flushing with double lumen needle in patients undergoing assisted reproductive technology treatments","volume":"25","author":"Mancebo","year":"2021","journal-title":"JBRA Assist. Reprod."},{"key":"ref_3","unstructured":"(2025, May 07). Wallace Single Lumen Oocyte Recovery Systems. Available online: https:\/\/www.coopersurgical.com\/product\/wallace-single-lumen-oocyte-recovery-systems\/."},{"key":"ref_4","unstructured":"(2025, May 07). Wallace Dual Lumen Oocyte Recovery Systems. Available online: https:\/\/www.coopersurgical.com\/product\/wallace-dual-lumen-oocyte-recovery-systems\/."},{"key":"ref_5","first-page":"105","article-title":"Introduction of the single-lumen oocyte retrieval needle in an assisted reproductive technology center: Propensity score matching analysis of grouping Taiwan","volume":"64","author":"Huang","year":"2025","journal-title":"J. Obs. Gynecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1093\/humrep\/dex019","article-title":"Randomized, open trial comparing a modified double-lumen needle follicular flushing system with a single-lumen aspiration needle in IVF patients with poor ovarian response","volume":"32","author":"Marion","year":"2017","journal-title":"Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"167","DOI":"10.4236\/asm.2017.74014","article-title":"Double-Lumen Needle Follicular Flushing System versus Single-Lumen Aspiration Needle in IVF\/ICSI Patients with Poor Ovarian Response: A Meta-Analysis","volume":"7","author":"Weijie","year":"2017","journal-title":"Adv. Sex. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.fertnstert.2010.09.013","article-title":"In vitro fertilization\u2013intracytoplasmic sperm injection outcomes in single- versus double-lumen oocyte retrieval needles in normally responding patients: A randomized trial","volume":"95","author":"Bulent","year":"2011","journal-title":"Fertil. Steril."},{"key":"ref_9","unstructured":"Alejandro, C., and Gautam, N. (2011). Optimizing Technique in Follicular Aspiration and Flushing. Textbook of Minimal Stimulation IVF-Milder, Mildest or Back to Nature, Jaypee Brothers Medical Pub. [1st ed.]."},{"key":"ref_10","unstructured":"(2025, July 16). Poiseuille\u2019s Law. Available online: https:\/\/phys.libretexts.org\/@go\/page\/8532."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/humupd\/2.1.77","article-title":"Aspiration of oocytes for in-vitro fertilization","volume":"2","author":"Robert","year":"1996","journal-title":"Hum. Reprod. Update"},{"key":"ref_12","first-page":"423","article-title":"Optimising the semi natural cycle IVF: The importance of follicular flushing","volume":"104","author":"Lozano","year":"2006","journal-title":"J. Indian Med. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1097\/GCO.0b013e3283373bfe","article-title":"Is there a benefit in follicular flushing in assisted reproductive technology?","volume":"22","author":"Hill","year":"2010","journal-title":"Curr. Opin. Obs. Gynecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s10815-013-0124-9","article-title":"Correlation between Follicular Diameters and Flushing versus No Flushing on Oocyte Maturity, Fertilization Rate and Embryo Quality","volume":"31","author":"Mehri","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Yang, W., Meixiang, Z., Hao, S., Shiqi, Y., Qian, L., Yingchun, S., Guo, Y., Hu, L., Sun, J., and Sun, Y.-P. (2022). Causes and Effects of Oocyte Retrieval Difficulties: A Retrospective Study of 10,624 Cycles. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.564344"},{"key":"ref_16","first-page":"CD004634","article-title":"Follicular flushing during oocyte retrieval in assisted reproductive techniques","volume":"4","author":"Georgiou","year":"2018","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1097\/GCO.0000000000000870","article-title":"The importance of follicular flushing in optimizing oocyte retrieval","volume":"35","author":"Lainas","year":"2023","journal-title":"Curr. Opin. Obs. Gynecol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1007\/s10815-014-0334-9","article-title":"Approaches to oocyte retrieval for advanced reproductive technology cycles planning to utilize in vitro maturation: A review of the many choices to be made","volume":"31","author":"Rose","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1007\/s10815-012-9869-9","article-title":"Follicular flushing during oocyte retrieval: A systematic review and meta-analysis","volume":"29","author":"Matheus","year":"2012","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1093\/humrep\/des174","article-title":"The use of follicle flushing during oocyte retrieval in assisted reproductive technologies: A systematic review and meta-analysis","volume":"27","author":"Levy","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S1472-6483(10)60564-0","article-title":"Optimal reproductive competence of oocytes retrieved through follicular flushing in minimal stimulation IVF","volume":"16","author":"Mendez","year":"2008","journal-title":"Reprod. Biomed. Online"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1093\/humrep\/der106","article-title":"Association between the number of eggs and live birth in IVF treatment: An analysis of 400 135 treatment cycles","volume":"26","author":"Sesh","year":"2011","journal-title":"Hum. Reprod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/humupd\/dmr037","article-title":"The poor responder in IVF: Is the prognosis always poor? A systematic review","volume":"18","author":"Oudendijk","year":"2012","journal-title":"Hum. Reprod. Update"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0015-0282(16)54806-7","article-title":"A prospective study to investigate the value of flushing follicles during transvaginal ultrasound-directed follicle aspiration","volume":"57","author":"John","year":"1992","journal-title":"Fertil. Steril."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.fertnstert.2013.12.011","article-title":"Comparison of complication rates and pain scores after transvaginal ultrasound-guided oocyte pickup procedures for in vitro maturation and in vitro fertilization cycles","volume":"101","author":"Seyhan","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1080\/01443610500118970","article-title":"Is there a benefit from routine follicular flushing for oocyte retrieval?","volume":"25","author":"Bagtharia","year":"2005","journal-title":"J. Obs. Gynaecol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.fl.25.010193.000245","article-title":"The History of Poiseuille\u2019s Law","volume":"25","author":"Salvatore","year":"1993","journal-title":"Annu. Rev. Fluid. Mech."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/9\/1334\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:33:28Z","timestamp":1760034808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/9\/1334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,22]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["life15091334"],"URL":"https:\/\/doi.org\/10.3390\/life15091334","relation":{},"ISSN":["2075-1729"],"issn-type":[{"type":"electronic","value":"2075-1729"}],"subject":[],"published":{"date-parts":[[2025,8,22]]}}}