{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:27:25Z","timestamp":1763389645516,"version":"3.45.0"},"reference-count":55,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research, Innovation and Digitization, CCCDI-UEFISCDI","award":["ERANET-EURONANOMED-3, ANTINEUROPATHO within PNCDI III"],"award-info":[{"award-number":["ERANET-EURONANOMED-3, ANTINEUROPATHO within PNCDI III"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The spirochete Borrelia is responsible for Lyme disease, a multisystemic infection and growing public health concern. This study aimed to evaluate host response dynamics to Borrelia bavariensis by analyzing hematological parameters as potential immuno-inflammatory markers in a murine model. Forty C3He\/HeNCrl mice were inoculated intradermally with B. bavariensis (5 \u00d7 105 spirochetes\/100 \u00b5L\/mouse) and monitored for 90 days. Samples were collected at defined intervals for microbiological examination, hematology, and qPCR. Microbiological and qPCR testing revealed infection between days 7\u201321; results were negative on days 28\u201342. At later stages (days 60 and 90), Borrelia was only detectable by qPCR, highlighting differences in diagnostic sensitivity. Hematological analysis showed that the neutrophil-to-lymphocyte ratio (NLR) and systemic immuno-inflammatory index (SII) peaked on day 7 (p &lt; 0.0001), followed by gradual normalization until day 35. These markers reflected the intensity of the inflammatory response and defined three distinct phases of host reaction. Overall, results demonstrate the complexity of immune responses in B. bavariensis infection and underscore the value of monitoring hematological indices for understanding host\u2013pathogen interactions. This approach supports the potential use of simple blood markers in diagnostic strategies with translational relevance for clinical practice.<\/jats:p>","DOI":"10.3390\/life15111758","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:04:07Z","timestamp":1763388247000},"page":"1758","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Immune Response Dynamics: Analyzing the Parameters of Complete Blood Count (CBC) in Experimental Borreliosis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-8298","authenticated-orcid":false,"given":"Diana Mihaela","family":"Alexandru","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, 050097 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6985-5152","authenticated-orcid":false,"given":"Diana Larisa","family":"Ancu\u0163a","sequence":"additional","affiliation":[{"name":"Cantacuzino National Military-Medical Institute for Research and Development, 050096 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6037-183X","authenticated-orcid":false,"given":"Cristin","family":"Coman","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, 050097 Bucharest, Romania"},{"name":"Cantacuzino National Military-Medical Institute for Research and Development, 050096 Bucharest, Romania"},{"name":"Center of Excellence in Translational Medicine, Fundeni Clinical Institute, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Thippani, S., Patel, N.J., Jathan, J., Filush, K., Socarras, K.M., DiLorenzo, J., Balasubramanian, K., Gupta, K., Ortiz Aleman, G., and Pandya, J.M. 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