{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T05:15:22Z","timestamp":1764738922514,"version":"3.46.0"},"reference-count":81,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002462","name":"Chungnam National University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002462","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>ASFV is responsible for high mortality in domestic pigs and has caused substantial economic impact on the global swine industry due to herd losses, trade restrictions, and disease control measures. We analyzed publicly available spleen RNA-seq data from ASFV-infected pigs (n = 13 total samples), including 7 pre-infection (0 dpi), 4 samples at 2 days post-infection (2 dpi), and 2 samples at 5 dpi (5 dpi). Weighted gene co-expression network analysis (WGCNA) identified 19 modules; module\u2013trait correlations revealed six modules associated with infection time. A co-expression module enriched for innate immune and antiviral response genes was strongly upregulated at 2 dpi, whereas a module enriched for ribosomal, translation, and metabolic process genes was broadly downregulated at 5 dpi. Protein\u2013protein interaction analysis highlighted hub genes, including EPRS1 and USP7 within core cellular\/translation programs and CMPK2 and ZBP1 within innate-immune signaling. Collectively, our results provide a network-level view of dynamic host responses and indicate coordinated shifts in immune and metabolic programs over time. These results identify CMPK2, ZBP1, EPRS1, and USP7 as hypothesis-generating hub gene candidates, warranting further validation to establish mechanistic roles and assess potential translational relevance.<\/jats:p>","DOI":"10.3390\/life15121844","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T12:39:43Z","timestamp":1764679183000},"page":"1844","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Gene Network Alterations and Identification of Key Genes in the Spleen During African Swine Fever Virus (ASFV) Infection"],"prefix":"10.3390","volume":"15","author":[{"given":"Jae-Beom","family":"Go","sequence":"first","affiliation":[{"name":"Department of Animal Resources Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vuong Nghia","family":"Bui","sequence":"additional","affiliation":[{"name":"Virology Department, National Institute of Veterinary Research, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duy Tung","family":"Dao","sequence":"additional","affiliation":[{"name":"Virology Department, National Institute of Veterinary Research, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngoc Anh","family":"Bui","sequence":"additional","affiliation":[{"name":"Virology Department, National Institute of Veterinary Research, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9705-2979","authenticated-orcid":false,"given":"Jihye","family":"Cha","sequence":"additional","affiliation":[{"name":"National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu Suk","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dajeong","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Animal Resources Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of Korea"},{"name":"Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s40813-015-0013-y","article-title":"African swine fever: A global view of the current challenge","volume":"1","author":"Gallardo","year":"2015","journal-title":"Porc. 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