{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T14:28:19Z","timestamp":1780410499102,"version":"3.54.1"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSRF via the Program Management Unit for Human Resources & Institutional Development, Research and Innovation","award":["B13F680126"],"award-info":[{"award-number":["B13F680126"]}]},{"name":"Franco-Thai Young Talent Research Fellowship Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Acne vulgaris is a common chronic inflammatory skin condition. Conventional acne treatments are often limited by adverse effects, driving interest in alternative therapies. This study explored the multifunctional bioactivities of a lyophilized cell-free supernatant (LCFS) derived from Lacticaseibacillus paracasei T0901, isolated from fermented palm sap, with a focus on its antimicrobial, antibiofilm, and antimelanogenic potential for dermatological applications. Antimicrobial activity was evaluated using agar well diffusion and broth microdilution assays against acne-associated pathogens, while antibiofilm effects were quantified via crystal violet staining. Antimelanogenic activity was assessed in \u03b1-melanocyte-stimulating hormone (\u03b1-MSH)-stimulated B16F10 melanoma cells by measuring melanin content and tyrosinase activity. Whole-genome sequencing was performed to identify genes linked to observed bioactivities, and molecular docking was used to predict metabolite\u2013protein interactions. The LCFS exhibited strong inhibitory activity against acne-associated bacteria, with inhibition zones of C. acnes (10.67 \u00b1 0.58 mm), S. epidermidis (21.00 \u00b1 0.00 mm), and S. aureus (20.00 \u00b1 0.00 mm), and a minimum inhibitory concentration of 25 mg\/mL. Biofilm formation was significantly reduced by 62.98 \u00b1 3.54%. In \u03b1-MSH-stimulated B16F10 cells, LCFS treatment (10 mg\/mL) significantly decreased melanin content (73.23 \u00b1 2.36%) and intracellular tyrosinase activity (68.19 \u00b1 6.29%) relative to control. Genomic analysis revealed antioxidant-related genes (sodA, trxB, nox), pigmentation regulators (mco, fcbD), and buk (butyrate kinase), supporting the observed bioactivities. Molecular docking further demonstrated strong binding affinities of LCFS-derived metabolites to tyrosinase and MITF, suggesting modulation of melanogenic pathways. Collectively, these results indicate that L. paracasei T0901 produces safe postbiotic compounds with potent antimicrobial, antibiofilm, and antimelanogenic activities, highlighting its promise as a multifunctional ingredient in probiotic-based skincare formulations.<\/jats:p>","DOI":"10.3390\/life15121866","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T10:50:38Z","timestamp":1764931838000},"page":"1866","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lyophilized Cell-Free Supernatants of Lacticaseibacillus paracasei T0901 Isolated from Fermented Palm Sap Exhibit Antiacne and Antimelanogenic Activities in B16F10 Melanoma Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6992-033X","authenticated-orcid":false,"given":"Phoomjai","family":"Sornsenee","sequence":"first","affiliation":[{"name":"Department of Family and Preventive Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-854X","authenticated-orcid":false,"given":"Nateelak","family":"Kooltheat","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawanwat C.","family":"Pattaranggoon","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology, Rangsit University, Muang Pathumthani, Pathumthani 12000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7793-7561","authenticated-orcid":false,"given":"Komwit","family":"Surachat","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1632-7318","authenticated-orcid":false,"given":"Arnaud","family":"Monteil","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Montpellier, CNRS, INSERM, 34090 Montpellier, France"},{"name":"Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1403-1628","authenticated-orcid":false,"given":"Chonticha","family":"Romyasamit","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Research Center in Tropical Pathobiology, Walailak University,  Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Innovation of Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e945336","DOI":"10.12659\/MSM.945336","article-title":"Skin Barrier Dysfunction in Acne Vulgaris: Pathogenesis and Therapeutic Approaches","volume":"30","author":"Deng","year":"2024","journal-title":"Med. Sci. Monit."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.det.2011.09.001","article-title":"Acne vulgaris: Pathogenesis, treatment, and needs assessment","volume":"30","author":"Dawson","year":"2012","journal-title":"Dermatol. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Karkoszka, M., Rok, J., and Wrze\u015bniok, D. (2024). Melanin Biopolymers in Pharmacology and Medicine-Skin Pigmentation Disorders, Implications for Drug Action, Adverse Effects and Therapy. Pharmaceuticals, 17.","DOI":"10.3390\/ph17040521"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1111\/j.1751-1097.2007.00226.x","article-title":"The protective role of melanin against UV damage in human skin","volume":"84","author":"Brenner","year":"2008","journal-title":"Photochem. Photobiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/biof.29","article-title":"Physiological factors that regulate skin pigmentation","volume":"35","author":"Yamaguchi","year":"2009","journal-title":"BioFactors"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Guermazi, D., and Saliba, E. (2025). The Genetics and Evolution of Human Pigmentation. Biology, 14.","DOI":"10.3390\/biology14081026"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1159\/000359974","article-title":"Niacinamide\u2014Mechanisms of action and its topical use in dermatology","volume":"27","author":"Wohlrab","year":"2014","journal-title":"Skin Pharmacol. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.biopha.2018.12.006","article-title":"Kojic acid applications in cosmetic and pharmaceutical preparations","volume":"110","author":"Saeedi","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/14756366.2016.1256882","article-title":"Skin whitening agents: Medicinal chemistry perspective of tyrosinase inhibitors","volume":"32","author":"Pillaiyar","year":"2017","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/ics.12763","article-title":"Natural Sources of Melanogenic Inhibitors: A Systematic Review","volume":"44","author":"Neto","year":"2022","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Puebla-Barragan, S., and Reid, G. (2021). Probiotics in Cosmetic and Personal Care Products: Trends and Challenges. Molecules, 26.","DOI":"10.3390\/molecules26051249"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zeng, M., Li, Y., Cheng, J., Wang, J., and Liu, Q. (2025). Prebiotic Oligosaccharides in Skin Health: Benefits, Mechanisms, and Cosmetic Applications. Antioxidants, 14.","DOI":"10.3390\/antiox14060754"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"235","DOI":"10.17221\/35\/2025-CJFS","article-title":"The potential of probiotics derived from functional foods for skin health","volume":"43","author":"Son","year":"2025","journal-title":"Czech J. Food Sci."},{"key":"ref_14","unstructured":"(2025, November 01). Future Market Insights. Probiotic Skincare Ingredients Market. 12 October 2025. Available online: https:\/\/www.futuremarketinsights.com\/reports\/probiotic-skincare-ingredients-market."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic","volume":"11","author":"Hill","year":"2014","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1038\/s41591-019-0439-x","article-title":"The pros, cons, and many unknowns of probiotics","volume":"25","author":"Suez","year":"2019","journal-title":"Nat. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e16703","DOI":"10.1111\/jocd.16703","article-title":"Efficacy of a Postbiotic Formulation Combined With Microneedling for Mild-to-Moderate Acne: A Self-Control Study","volume":"24","author":"Li","year":"2025","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e12586","DOI":"10.7717\/peerj.12586","article-title":"Lyophilized cell-free supernatants of Lactobacillus isolates exhibited antibiofilm, antioxidant, and reduces nitric oxide activity in lipopolysaccharide-stimulated RAW 264.7 cells","volume":"9","author":"Sornsenee","year":"2021","journal-title":"PeerJ"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sornsenee, P., Kooltheat, N., Wongprot, D., Suksabay, P., Nam, T.G., Permpoon, U., Saengsuwan, P., and Romyasamit, C. (2025). Antibacterial, Antioxidant, and Anti-inflammatory Activities of Lacticaseibacillus paracasei Lysates Isolated from Fermented Palm Sap. Probiotics Antimicrob Proteins.","DOI":"10.1007\/s12602-025-10521-6"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Machado, P., Ribeiro, F.N., Giublin, F.C.W., Mieres, N.G., Tonin, F.S., Pontarolo, R., Sari, M.H.M., Lazo, R.E.L., and Ferreira, L.M. (2025). Next-Generation Wound Care: A Scoping Review on Probiotic, Prebiotic, Synbiotic, and Postbiotic Cutaneous Formulations. Pharmaceuticals, 18.","DOI":"10.3390\/ph18050704"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s12223-019-00759-3","article-title":"Probiotics and prebiotics potential for the care of skin, female urogenital tract, and respiratory tract","volume":"65","author":"Bustamante","year":"2020","journal-title":"Folia Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/exd.14032","article-title":"The role of topical probiotics in skin conditions: A systematic review of animal and human studies and implications for future therapies","volume":"29","author":"Knackstedt","year":"2020","journal-title":"Exp. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"adv33206","DOI":"10.2340\/actadv.v104.33206","article-title":"A Randomized Clinical Trial to Evaluate the Efficacy of an Oral Probiotic in Acne Vulgaris","volume":"104","author":"Eguren","year":"2024","journal-title":"Acta Derm.-Venereol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, Y., Hu, X., Dong, G., Wang, X., and Liu, T. (2024). Acne treatment: Research progress and new perspectives. Front. Med., 11.","DOI":"10.3389\/fmed.2024.1425675"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.7150\/ijms.51545","article-title":"Regulatory effects of Lactobacillus plantarum-GMNL6 on human skin health by improving skin microbiome","volume":"18","author":"Tsai","year":"2021","journal-title":"Int. J. Med. Sci."},{"key":"ref_26","first-page":"237","article-title":"Antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 and APsulloc 331266 against pathogenic skin microbiota","volume":"13","author":"Chae","year":"2021","journal-title":"FBE"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"990","DOI":"10.4014\/jmb.2104.04008","article-title":"Antioxidant and Antimelanogenic Activities of Kimchi-Derived Limosilactobacillus fermentum JNU532 in B16F10 Melanoma Cells","volume":"31","author":"Meng","year":"2021","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lee, S., Park, H.O., and Yoo, W. (2022). Anti-Melanogenic and Antioxidant Effects of Cell-Free Supernatant from Lactobacillus gasseri BNR17. Microorganisms, 10.","DOI":"10.3390\/microorganisms10040788"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1007\/s12602-021-09754-y","article-title":"Probiotic Properties of Lactobacillus Species Isolated from Fermented Palm Sap in Thailand","volume":"13","author":"Sornsenee","year":"2021","journal-title":"Probiotics Antimicrob Proteins"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Romyasamit, C., Thatrimontrichai, A., Aroonkesorn, A., Chanket, W., Ingviya, N., Saengsuwan, P., and Singkhamanan, K. (2020). Enterococcus faecalis Isolated From Infant Feces Inhibits Toxigenic Clostridioides (Clostridium) difficile. Front. Pediatr., 8.","DOI":"10.3389\/fped.2020.572633"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Li, M., Liu, Z., He, J., Jiang, J., Shang, D., and Dong, W. (2025). Trp-containing Peptides with Therapeutic Potential for Cutibacterium acnes Infection. Probiotics Antimicrob. Proteins.","DOI":"10.1007\/s12602-025-10749-2"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yang, K.M., Kim, J.-S., Kim, H.-S., Kim, Y.-Y., Oh, J.-K., Jung, H.-W., Park, D.-S., and Bae, K.-H. (2021). Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria. Sci. Rep., 11.","DOI":"10.1038\/s41598-020-80921-x"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Perumal, S., and Mahmud, R. (2013). Chemical analysis, inhibition of biofilm formation and biofilm eradication potential of Euphorbia hirta L. against clinical isolates and standard strains. BMC Complement. Altern. Med., 13.","DOI":"10.1186\/1472-6882-13-346"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"102680","DOI":"10.1016\/j.psj.2023.102680","article-title":"The elastase and melanogenesis inhibitory and anti-inflammatory activities of phosvitin phosphopeptides produced using high-temperature and mild-pressure (HTMP) pretreatment and enzyme hydrolysis combinations","volume":"102","author":"Lee","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2515690X231152928","DOI":"10.1177\/2515690X231152928","article-title":"Melanogenesis Inhibitory Activity, Chemical Components and Molecular Docking Studies of Prunus cerasoides Buch.-Ham. D. Don. Flowers","volume":"28","author":"Kooltheat","year":"2023","journal-title":"J. Evid.-Based Integr. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.4014\/jmb.2408.08015","article-title":"Antimelanogenic and Antioxidant Effects of Postbioics of Lactobacillus Strains in \u03b1-MSH-Induced B16F10 Melanoma Cells via CREB\/MITF and MAPKs Signaling Pathway","volume":"34","author":"Lee","year":"2024","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W278","DOI":"10.1093\/nar\/gky383","article-title":"BAGEL4: A user-friendly web server to thoroughly mine RiPPs and bacteriocins","volume":"46","author":"Song","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: Massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"Varadi","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D368","DOI":"10.1093\/nar\/gkad1011","article-title":"AlphaFold Protein Structure Database in 2024: Providing structure coverage for over 214 million protein sequences","volume":"52","author":"Varadi","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1038\/s41596-020-0312-x","article-title":"The HDOCK server for integrated protein\u2013protein docking","volume":"15","author":"Yan","year":"2020","journal-title":"Nat. Protoc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W443","DOI":"10.1093\/nar\/gkv315","article-title":"PLIP: Fully automated protein-ligand interaction profiler","volume":"43","author":"Salentin","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00253-021-11646-8","article-title":"Probiotics, prebiotics and synbiotics: Safe options for next-generation therapeutics","volume":"106","author":"Yadav","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/nutrit\/nuy056","article-title":"Yogurt and other fermented foods as sources of health-promoting bacteria","volume":"76","author":"Kok","year":"2018","journal-title":"Nutr. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kullar, R., Goldstein, E.J.C., Johnson, S., and McFarland, L.V. (2023). Lactobacillus Bacteremia and Probiotics: A Review. Microorganisms, 11.","DOI":"10.3390\/microorganisms11040896"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"566","DOI":"10.2174\/1389201021666200109104701","article-title":"Lactic Acid Bacteria and Lactic Acid for Skin Health and Melanogenesis Inhibition","volume":"21","author":"Huang","year":"2020","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"566","DOI":"10.36849\/JDD.8648","article-title":"A Review of the Role of C. Acnes and its Biofilm in Dandruff Pathogenesis","volume":"24","author":"Scott","year":"2025","journal-title":"J. Drugs Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kreouzi, M., Theodorakis, N., Nikolaou, M., Feretzakis, G., Anastasiou, A., Kalodanis, K., and Sakagianni, A. (2025). Skin Microbiota: Mediator of Interactions Between Metabolic Disorders and Cutaneous Health and Disease. Microorganisms, 13.","DOI":"10.3390\/microorganisms13010161"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Salini, F., Iacumin, L., Comi, G., and Dicks, L.M.T. (2023). Thermophilin 13: In Silico Analysis Provides New Insight in Genes Involved in Bacteriocin Production. Microorganisms., 11.","DOI":"10.3390\/microorganisms11030611"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1111\/j.1574-6976.2007.00080.x","article-title":"Carnobacterium: Positive and negative effects in the environment and in foods","volume":"31","author":"Leisner","year":"2007","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Wu, Y., Pang, X., Wu, Y., Liu, X., and Zhang, X. (2022). Enterocins: Classification, Synthesis, Antibacterial Mechanisms and Food Applications. Molecules, 27.","DOI":"10.3390\/molecules27072258"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"112451","DOI":"10.1016\/j.lwt.2021.112451","article-title":"Purification, characterization and mechanism of action of enterocin HDX-2, a novel class IIa bacteriocin produced by Enterococcus faecium HDX-2","volume":"153","author":"Du","year":"2022","journal-title":"LWT"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Sornsenee, P., Surachat, K., Kang, D.-K., Mendoza, R., and Romyasamit, C. (2024). Probiotic Insights from the Genomic Exploration of Lacticaseibacillus paracasei Strains Isolated from Fermented Palm Sap. Foods, 13.","DOI":"10.3390\/foods13111773"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"106598","DOI":"10.1016\/j.micpath.2024.106598","article-title":"Antibacterial activity and mechanism of cell-free supernatants of Lacticaseibacillus paracasei against Propionibacterium acnes","volume":"189","author":"Xu","year":"2024","journal-title":"Microb. Pathog."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"D\u2019Mello, S.A., Finlay, G.J., Baguley, B.C., and Askarian-Amiri, M.E. (2016). Signaling pathways in melanogenesis. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071144"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Xiao, X., Hu, X., Yao, J., Cao, W., Zou, Z., Wang, L., Qin, H., Zhong, D., Li, Y., and Xue, P. (2022). The role of short-chain fatty acids in inflammatory skin diseases. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1083432"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Di Chiano, M., Rocchetti, M.T., Spano, G., Russo, P., Allegretta, C., Milior, G., Gadaleta, R.M., Sallustio, F., Pontrelli, P., and Gesualdo, L. (2024). Lactobacilli Cell-Free Supernatants Modulate Inflammation and Oxidative Stress in Human Microglia via NRF2-SOD1 Signaling. Cell. Mol. Neurobiol., 44.","DOI":"10.1007\/s10571-024-01494-1"}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/12\/1866\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:12:47Z","timestamp":1764936767000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/15\/12\/1866"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,5]]},"references-count":56,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["life15121866"],"URL":"https:\/\/doi.org\/10.3390\/life15121866","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,5]]}}}