{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:50:23Z","timestamp":1783133423623,"version":"3.54.6"},"reference-count":47,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State assignment","award":["FURG-2023-0096"],"award-info":[{"award-number":["FURG-2023-0096"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Alterations of pancreatic islet cell phenotypes are well established in diabetic conditions and considered to be one of the possible causes of insulin deficiency. However, there is limited information about alterations of islet cell phenotypes in opposite metabolic conditions such as hypoglycemia in infants with congenital hyperinsulinism (CHI). Surgical biopsies of the pancreas from six infants with diffuse CHI and five infants with focal CHI were examined using double immunofluorescence with antibodies against insulin, glucagon and the key transcriptional factor responsible for \u03b2-cell differentiation and maturation\u2014PDX1. The phenotypes of cells within the pancreatic islets in diffuse CHI and within the focus in focal CHI were compared to those in unaltered pancreatic islets located outside the focus. In diffuse CHI, the proportion of bi-hormonal insulin+\/glucagon+ cells was increased. Additionally, an increase in the proportion of insulin+ cells lacking PDX1 was observed in diffuse CHI and within the focus. It can be assumed that alterations of the phenotype of \u03b2-cells may occur under hypoglycemic conditions, but the role of islet cell plasticity in infants with CHI remains to be established.<\/jats:p>","DOI":"10.3390\/life16010018","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T11:30:18Z","timestamp":1766489418000},"page":"18","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cells Co-Producing Insulin and Glucagon in Congenital Hyperinsulinism"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9692-3616","authenticated-orcid":false,"given":"Yuliya","family":"Krivova","sequence":"first","affiliation":[{"name":"Laboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of FSBSI \u201cPetrovsky National Research Centre of Surgery\u201d, Tsurupi Street, 3, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0515-8275","authenticated-orcid":false,"given":"Alexandra","family":"Proshchina","sequence":"additional","affiliation":[{"name":"Laboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of FSBSI \u201cPetrovsky National Research Centre of Surgery\u201d, Tsurupi Street, 3, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6719-3383","authenticated-orcid":false,"given":"Dmitry","family":"Otlyga","sequence":"additional","affiliation":[{"name":"Laboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of FSBSI \u201cPetrovsky National Research Centre of Surgery\u201d, Tsurupi Street, 3, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diliara","family":"Gubaeva","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Endocrinology; Dm.Ulyanova St., 11, 117292 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Melikyan","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Endocrinology; Dm.Ulyanova St., 11, 117292 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Saveliev","sequence":"additional","affiliation":[{"name":"Laboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of FSBSI \u201cPetrovsky National Research Centre of Surgery\u201d, Tsurupi Street, 3, 117418 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1002\/ajmg.c.31737","article-title":"Congenital Hyperinsulinism Disorders: Genetic and Clinical Characteristics","volume":"181","author":"Rosenfeld","year":"2019","journal-title":"Proceedings of the American Journal of Medical Genetics Part C: Seminars in Medical Genetics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1159\/000526442","article-title":"Congenital Hyperinsulinism: An Historical Perspective","volume":"95","author":"Thornton","year":"2022","journal-title":"Horm. 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