{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T22:10:44Z","timestamp":1769551844725,"version":"3.49.0"},"reference-count":25,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Objective: This study aimed to investigate the relationship between bronchoalveolar lavage (BAL) cellular profiles, microbiological status, and clinical outcomes such as hospital admission in adult patients with non-cystic fibrosis bronchiectasis. Methods: A retrospective cross-sectional study was conducted on thirty adult bronchiectasis patients. Demographic, clinical, and laboratory data were collected. The cellular components of BAL fluid (macrophages, neutrophils, lymphocytes, and eosinophils) were analyzed. Patients were grouped according to the presence of microbial culture growth and history of hospitalization in the past year. Statistical analyses were performed to determine significant relationships. Results: The median age was 57 years, and the gender distribution was equal. There was no significant difference in BAL cellular profiles between groups with and without culture growth. However, in the group with a hospital admission in the past year, BAL showed a significantly lower percentage of alveolar macrophages (20% vs. 47%, p = 0.011) and a higher percentage of eosinophils (5% vs. 1%, p = 0.036). The hospitalized group also showed a trend toward a higher neutrophil percentage and a lower lymphocyte\/neutrophil ratio. Furthermore, surprising associations were noted, such as a higher BAL macrophage count in married individuals and higher BAL eosinophilia in patients with diabetes. Conclusions: BAL cellular analysis provides valuable information beyond routine microbiological investigations in bronchiectasis. The low-alveolar-macrophage and high-eosinophil profile was found to be significantly associated with hospitalization, and this profile has the potential to serve as a prognostic biomarker in defining the \u201chigh-risk\u201d phenotype. These findings highlight the complexity of the local inflammatory response and reveal the potential role of BAL in developing personalized treatment strategies for patients with bronchiectasis.<\/jats:p>","DOI":"10.3390\/life16020206","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:05:27Z","timestamp":1769504727000},"page":"206","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Prognostic Significance of Bronchoalveolar Lavage Cellular Analysis in Evaluating Disease Burden in Non-Cystic Fibrosis Bronchiectasis"],"prefix":"10.3390","volume":"16","author":[{"given":"Ahmet","family":"Yurtta\u015f","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Pulmonology, Alanya Alaaddin Keykubat University, 07425 Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4634-205X","authenticated-orcid":false,"given":"Deniz","family":"\u00c7elik","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Pulmonology, Alanya Alaaddin Keykubat University, 07425 Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sertan","family":"Bulut","sequence":"additional","affiliation":[{"name":"Ankara Atat\u00fcrk Sanatorium Training and Research Hospital, 06290 Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6zkan","family":"Yetkin","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Pulmonology, Alanya Alaaddin Keykubat University, 07425 Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00fcseyin","family":"Lakadamyal\u0131","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Pulmonology, Alanya Alaaddin Keykubat University, 07425 Alanya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2400518","DOI":"10.1183\/13993003.00518-2024","article-title":"Bronchiectasis management in adults: State of the art and future directions","volume":"63","author":"Choi","year":"2024","journal-title":"Eur. Respir. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1177\/1479972317709649","article-title":"Prevalence and incidence of noncystic fibrosis bronchiectasis among US adults in 2013","volume":"14","author":"Weycker","year":"2017","journal-title":"Chron. Respir. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chandrasekaran, R., Mac Aog\u00e1in, M., Chalmers, J.D., Elborn, S.J., and Chotirmall, S.H. (2018). Geographic variation in the aetiology, epidemiology and microbiology of bronchiectasis. BMC Pulm. Med., 18.","DOI":"10.1186\/s12890-018-0638-0"},{"key":"ref_4","first-page":"100006","article-title":"Diagnostic evaluation of bronchiectasis","volume":"1","author":"Chan","year":"2019","journal-title":"Respir. Med. X"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s004080000032","article-title":"Use of bronchoalveolar lavage in humans\u2014Past necessity and future imperative","volume":"178","author":"Reynolds","year":"2000","journal-title":"Lung"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1053\/rmed.2001.1153","article-title":"Computer program supporting the diagnostic accuracy of cellular BALF analysis: A new release","volume":"95","author":"Drent","year":"2001","journal-title":"Respir. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1136\/thx.53.8.685","article-title":"Increases in CD4+ T lymphocytes, macrophages, neutrophils and interleukin 8 positive cells in the airways of patients with bronchiectasis","volume":"53","author":"Gaga","year":"1998","journal-title":"Thorax"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1164\/rccm.201203-0487OC","article-title":"Short- and long-term antibiotic treatment reduces airway and systemic inflammation in non-cystic fibrosis bronchiectasis","volume":"186","author":"Chalmers","year":"2012","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1080\/0886022X.2020.1832521","article-title":"Neutrophil-to-lymphocyte ratio in relation to the risk of all-cause mortality and cardiovascular events in patients with chronic kidney disease: A systematic review and meta-analysis","volume":"42","author":"Zhao","year":"2020","journal-title":"Ren. Fail."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"48","DOI":"10.5578\/tt.69231","article-title":"Bronchiectasis in Turkey: Under the light of national publications","volume":"68","year":"2020","journal-title":"Tuberk. Toraks"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Clementi, C.F., and Murphy, T.F. (2011). Non-typeable Haemophilus influenzae invasion and persistence in the human respiratory tract. Front. Cell Infect. Microbiol., 1.","DOI":"10.3389\/fcimb.2011.00001"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S49","DOI":"10.1164\/ajrccm\/138.6_Pt_2.S49","article-title":"The effect of bacterial products on ciliary function","volume":"138","author":"Wilson","year":"1988","journal-title":"Am. Rev. Respir. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1080\/07853890.2021.1900594","article-title":"The contribution of Pseudomonas aeruginosa infection to clinical outcomes in bronchiectasis: A prospective cohort study","volume":"53","author":"Wang","year":"2021","journal-title":"Ann. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1513\/AnnalsATS.201506-333OC","article-title":"A comprehensive analysis of the impact of Pseudomonas aeruginosa colonization in bronchiectasis","volume":"12","author":"Finch","year":"2015","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_15","first-page":"576","article-title":"Characterization of bacterial community diversity in non\u2013cystic fibrosis bronchiectasis","volume":"189","author":"Rogers","year":"2014","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_16","first-page":"121","article-title":"Use of culture and molecular analysis to determine bacterial diversity in the lungs of patients with bronchiectasis","volume":"68","author":"Tunney","year":"2013","journal-title":"Thorax"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Purcell, P., Jary, H., Perry, A., Perry, J.D., Stewart, C.J., Nelson, A., Lanyon, C., Smith, D.L., Cummings, S.P., and De Soyza, A. (2014). Polymicrobial airway bacterial communities in adult bronchiectasis patients. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-130"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1055\/s-0041-1730891","article-title":"Pathophysiology of Bronchiectasis","volume":"42","author":"Keir","year":"2021","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1136\/thx.39.6.408","article-title":"Elastolytic activity of sputum and its relation to purulence and to lung function in patients with bronchiectasis","volume":"39","author":"Stockley","year":"1984","journal-title":"Thorax"},{"key":"ref_20","first-page":"297","article-title":"Immunopathology of Experimental Bronchiectasis","volume":"1","author":"Guerreiro","year":"1989","journal-title":"Cell Mol. Bioi."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/j.1749-6632.1994.tb39816.x","article-title":"Cells and Cytokines in Chronic Bronchial Infection","volume":"725","author":"Eller","year":"1994","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1136\/thx.2006.061358","article-title":"Innate immune activation in neutrophilic asthma and bronchiectasis","volume":"62","author":"Simpson","year":"2007","journal-title":"Thorax"},{"key":"ref_23","unstructured":"Global Initiative for Chronic Obstructive Lung Disease (GOLD) (2025). Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease, Global Initiative for Chronic Obstructive Lung Disease. 2025 Report."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.hrtlng.2016.10.002","article-title":"The palliative care needs of patients with idiopathic pulmonary fibrosis: A qualitative study of patients and family caregivers","volume":"46","author":"Lindell","year":"2017","journal-title":"Heart Lung"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1365-2710.2006.00708.x","article-title":"Effects of clarithromycin on inflammatory parameters and clinical conditions in children with bronchiectasis","volume":"31","author":"Kiper","year":"2006","journal-title":"J. Clin. Pharm. Ther."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/2\/206\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:22:39Z","timestamp":1769505759000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/2\/206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["life16020206"],"URL":"https:\/\/doi.org\/10.3390\/life16020206","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}