{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T13:43:26Z","timestamp":1778507006301,"version":"3.51.4"},"reference-count":74,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002367","name":"Strategic Priority Research Program of Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["XDA2301040103"],"award-info":[{"award-number":["XDA2301040103"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chun Hui Talent Program of Hebei Province","award":["C2022112002"],"award-info":[{"award-number":["C2022112002"]}]},{"name":"Innovation Capability Enhancement Plan of Hebei Province","award":["235A3801D"],"award-info":[{"award-number":["235A3801D"]}]},{"DOI":"10.13039\/501100015260","name":"Macau University of Science and Technology","doi-asserted-by":"crossref","award":["002\/2024\/SKL"],"award-info":[{"award-number":["002\/2024\/SKL"]}],"id":[{"id":"10.13039\/501100015260","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42377126"],"award-info":[{"award-number":["42377126"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Petroleum contamination poses a serious threat to human health and ecosystems worldwide, and microbially driven natural attenuation is an effective approach for accelerating hydrocarbon removal. Species of the genus Rhodococcus are recognized for their ability to degrade long chain petroleum hydrocarbons. However, their physiological traits and degradation mechanisms under alkaline conditions remain insufficiently understood. In this study, soil samples were collected from the Dagang oilfield in Tianjin, China, and Rhodococcus sp. A17 was isolated as an active indigenous strain for genomic and physiological characterization under high pH petroleum degradation conditions. The results showed that strain A17 grew optimally at 30 \u00b0C, pH 9.0, and 2% salinity. Petroleum hydrocarbon degradation reached 67.8% within 72 h, with a degradation half life of 34.2 h. Genome sequencing identified 18 oxygenase related genes involved in alkane degradation, including alkB, cytochrome P450 monooxygenases, and the long chain alkane monooxygenase ladA, together with four antibiotic resistance genes. Metabolite analysis suggested that alkane degradation might proceed via terminal and subterminal oxidation pathways. Overall, these findings indicate that Rhodococcus sp. A17 exhibits multiple adaptive traits that support its potential application in the bioremediation of petroleum contaminated alkaline environments.<\/jats:p>","DOI":"10.3390\/life16020252","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T12:00:47Z","timestamp":1770033647000},"page":"252","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Insights into Rhodococcus sp. A17: Physiological Adaptations and Degradation Characteristics for Organic Contamination at Alkaline pH"],"prefix":"10.3390","volume":"16","author":[{"given":"Xinyuan","family":"Wei","sequence":"first","affiliation":[{"name":"Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Xiong\u2019an Institute of Innovation, Chinese Academy of Sciences, Baoding 071700, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengmin","family":"Liu","sequence":"additional","affiliation":[{"name":"Dingzhou Fuyuan Food Co., Ltd., Dingzhou 073000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Zuo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Lunar and Planetary Sciences, Macau University of Science and Technology, Taipa 999078, Macau"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8785-1876","authenticated-orcid":false,"given":"Qing","family":"Hu","sequence":"additional","affiliation":[{"name":"Xiong\u2019an Institute of Innovation, Chinese Academy of Sciences, Baoding 071700, China"},{"name":"State Key Laboratory of Lunar and Planetary Sciences, Macau University of Science and Technology, Taipa 999078, Macau"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5902-4267","authenticated-orcid":false,"given":"Xuliang","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-527X","authenticated-orcid":false,"given":"Zhihui","family":"Bai","sequence":"additional","affiliation":[{"name":"Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Xiong\u2019an Institute of Innovation, Chinese Academy of Sciences, Baoding 071700, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"ref_1","first-page":"77","article-title":"Petroleum Pollution","volume":"3","author":"Wilson","year":"2000","journal-title":"Rev. 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