{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:08:18Z","timestamp":1772212098506,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Projects Commission of Afyon Kocatepe University","award":["17 Kariyer 190"],"award-info":[{"award-number":["17 Kariyer 190"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>In this study, the effects of carrot juice on performance, egg quality, blood parameters, and intestinal flora in laying hens were examined. One hundred twenty-eight Babcock white laying hens were divided into four random groups, each with four replicates of eight hens. Fresh carrot juice was introduced to the drinking water of the hens at concentrations of 0% (control), 1%, 2.5%, and 5% for a duration of four weeks. Weekly feed intake, egg weight, feed conversion ratio, and daily egg production were monitored, while yolk color, albumen and yolk indices, shell thickness, and Haugh units were measured in weeks two and four. Blood samples were analyzed for biochemical and physiological parameters, and fecal samples were analyzed for microbial parameters at the end of the study. Carrot juice improved egg production, egg weight, and egg mass, without affecting daily feed intake, feed conversion ratio, or water consumption, while enhancing yolk color but reducing eggshell thickness. Blood analyses showed higher levels of total oxidant status, vitamin E, immunoglobulin G, alkaline phosphatase, lymphocyte count, and mean corpuscular hemoglobin concentration. Moreover, fecal Enterococcus counts were positively affected by the treatment. The findings indicate that carrot juice supplementation could positively affect performance, egg quality, some immune-related blood parameters, and gut microbiota in laying hens. Among the levels evaluated, adding 2.5% carrot juice to drinking water was found to provide the most balanced response.<\/jats:p>","DOI":"10.3390\/life16030382","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T15:52:38Z","timestamp":1772207558000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Different Levels of Carrot Juice Supplementation in Drinking Water on Performance, Egg Quality, Blood Parameters, and Gut Health of Babcock White Laying Hens"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1143-1215","authenticated-orcid":false,"given":"Umit","family":"Ozcinar","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4663-081X","authenticated-orcid":false,"given":"Muhammet Emre","family":"Orman","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7803-2454","authenticated-orcid":false,"given":"Cangir","family":"Uyarlar","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2404-1232","authenticated-orcid":false,"given":"Eyup Eren","family":"Gultepe","sequence":"additional","affiliation":[{"name":"Kemin Europa NV, Toekomstlaan 42, 2200 Herentals, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6527-8420","authenticated-orcid":false,"given":"O\u011fuz Ka\u011fan","family":"T\u00fcredi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aamir","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130brahim Sadi","family":"\u00c7eting\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beytullah","family":"Kenar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismail","family":"Bayram","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"ref_1","first-page":"8","article-title":"Feed additives in poultry nutrition","volume":"25","author":"Pirgozliev","year":"2019","journal-title":"Bulg. J. Agric. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Abdelli, N., Sol\u00e0-Oriol, D., and P\u00e9rez, J.F. (2021). Phytogenic Feed Additives in Poultry: Achievements, Prospective and Challenges. Animals, 11.","DOI":"10.3390\/ani11123471"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/jpn.13922","article-title":"Assessing the potential of phytogenic feed additives: A comprehensive review on their effectiveness as a potent dietary enhancement for nonruminant in swine and poultry","volume":"108","author":"Biswas","year":"2024","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"470","DOI":"10.14202\/vetworld.2016.470-474","article-title":"Influence of combinations of fenugreek, garlic, and black pepper powder on production traits of the broilers","volume":"9","author":"Kirubakaran","year":"2016","journal-title":"Vet. World"},{"key":"ref_5","first-page":"369","article-title":"Application of botanical products as nutraceutical feed additives for improving poultry health and production","volume":"16","author":"Khalifah","year":"2023","journal-title":"Vet. World"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105303","DOI":"10.1016\/j.jff.2022.105303","article-title":"Critical review on the immunomodulatory activities of carrot\u2019s \u03b2-carotene and other bioactive compounds","volume":"99","author":"Anjani","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1080\/00071660701473857","article-title":"Effect of feeding silages or carrots as supplements to laying hens on production performance, nutrient digestibility, gut structure, gut microflora and feather pecking behaviour","volume":"48","author":"Steenfeldt","year":"2007","journal-title":"Br. Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1007\/s11250-023-03636-x","article-title":"The use of purple carrot powder in the diet of laying quails improved some egg quality characteristics, including antioxidant capacity","volume":"55","author":"Olgun","year":"2023","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_9","first-page":"12337","article-title":"Effect of dietary carrot meal supplementation on productivity and carcass characteristics of Arbor acre broiler chickens aged 22 to 42 days","volume":"17","author":"Mokgope","year":"2019","journal-title":"Appl. Ecol. Environ. Res."},{"key":"ref_10","first-page":"180","article-title":"Carcass and cutting yields, meat qualitative traits and sensory evaluation of broiler chickens fed diet contain clove and treated of carrot in drinking water","volume":"65","author":"Mandey","year":"2022","journal-title":"Sci. Pap. Ser. D Anim. Sci. Int. Sess. Sci. Commun. Fac. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/1475-2891-10-96","article-title":"Drinking carrot juice increases total antioxidant status and decreases lipid peroxidation in adults","volume":"10","author":"Potter","year":"2011","journal-title":"Nutr. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"331","DOI":"10.3889\/oamjms.2015.056","article-title":"Beetroot-Carrot Juice Intake either Alone or in Combination with Antileukemic Drug \u2018Chlorambucil\u2019 As A Potential Treatment for Chronic Lymphocytic Leukemia","volume":"3","author":"Shakib","year":"2015","journal-title":"Open Access Maced. J. Med. Sci."},{"key":"ref_13","unstructured":"NRC (1994). Nutrient Requirements of Poultry: Ninth Revised Edition, The National Academies Press."},{"key":"ref_14","first-page":"539","article-title":"Havu\u00e7 Cipsinde \u0130deal \u00dcretim Parametrelerinin Belirlenmesi","volume":"46","author":"Oral","year":"2021","journal-title":"G\u0131da"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"705","DOI":"10.5713\/ajas.17.0787","article-title":"Effect of golden needle mushroom (Flammulina velutipes) stem waste on laying performance, calcium utilization, immune response and serum immunity at early phase of production","volume":"31","author":"Mahfuz","year":"2018","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_16","first-page":"552","article-title":"The Haugh unit for measuring egg quality","volume":"43","author":"Haugh","year":"1937","journal-title":"United States Egg Poult. Mag."},{"key":"ref_17","first-page":"561","article-title":"Effects of storage time on external and internal characteristics in partridge (Alectoris graeca) eggs","volume":"155","author":"Tilki","year":"2004","journal-title":"Rev. Med. Vet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0034-5288(03)00036-5","article-title":"Effects of probiotics: Alone and in a mixture of Biosacc\u00ae plus Zinc Bacitracin on the caecal microflora of Japanese quail","volume":"75","author":"Siriken","year":"2003","journal-title":"Res. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"893","DOI":"10.4315\/0362-028X-57.10.893","article-title":"Microbiological Criteria of Random Samples of Spices and Herbs Retailed on the Austrian Market","volume":"57","author":"Kneifel","year":"1994","journal-title":"J. Food Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/j.1472-765X.2005.01749.x","article-title":"Effect of media, temperature and culture conditions on the species population and antibiotic resistance of enterococci from broiler chickens","volume":"41","author":"Jackson","year":"2005","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"910","DOI":"10.19113\/sdufbed.91790","article-title":"Farkl\u0131 kaynaklardan izole edilen Salmonella Su\u015flar\u0131n\u0131n Baz\u0131 Vir\u00fclans Fakt\u00f6rlerinin Belirlenmesi","volume":"21","author":"Kimiran","year":"2017","journal-title":"S\u00fcleyman Demirel \u00dcniversitesi Fen Bilim. Enstit\u00fcs\u00fc Derg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0966-842X(98)01430-9","article-title":"Clostridium perfringens: Toxinotype and genotype","volume":"7","author":"Petit","year":"1999","journal-title":"Trends Microbiol."},{"key":"ref_23","unstructured":"(2013). Microbiology of the Food Chain-Horizontal Method for the Enumeration of Microorganisms. Part 1:Colony Count at 30 \u00b0C by the Pour Plate Technique (Standard No. ISO 4833). Available online: https:\/\/www.iso.org\/standard\/53728.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.5713\/ajas.1998.239","article-title":"Use of dried carrot meal (DCM) in laying hen diet for egg yolk pigmentation","volume":"11","author":"Sikder","year":"1998","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"395","DOI":"10.17221\/54\/2019-CJAS","article-title":"Egg yolk colour and retinol concentration of eggs from laying hens fed diets containing carrot and beetroot meal","volume":"64","author":"Lima","year":"2019","journal-title":"Czech J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1080\/1828051X.2021.2002733","article-title":"Effects of dietary natural vitamin E supplementation on laying performance, egg quality, serum biochemical indices, tocopherol deposition and antioxidant capacity of laying hens","volume":"20","author":"Zhao","year":"2021","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104033","DOI":"10.1016\/j.psj.2024.104033","article-title":"Effect of dietary inclusion of 25-hydroxyvitamin D3 and vitamin E on performance, gut health, oxidative status, and immune response in laying hens infected with coccidiosis","volume":"103","author":"Sharma","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100184","DOI":"10.1016\/j.japr.2021.100184","article-title":"Effects of dietary vitamin E supplementation on laying performance, hatchability, and antioxidant status in molted broiler breeder hens","volume":"30","author":"Yang","year":"2021","journal-title":"J. Appl. Poult. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.abb.2013.07.009","article-title":"Biosynthesis of carotenoids in carrot: An underground story comes to light","volume":"539","author":"Stange","year":"2013","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"282","DOI":"10.5851\/kosfa.2022.e76","article-title":"Performance, Egg Quality, and Immunity of Laying Hens due to Natural Carotenoid Supplementation: A Meta-Analysis","volume":"43","author":"Yunitasari","year":"2023","journal-title":"Food Sci. Anim. Resour."},{"key":"ref_31","first-page":"415","article-title":"The Effect of Egg Weight on Egg External Quality Characteristics and Hatching Performance in Pekin Ducks","volume":"29","author":"Kirmizibayrak","year":"2023","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1111\/jpn.13375","article-title":"Health benefits of carotenoids and potential application in poultry industry: A review","volume":"104","author":"Nabi","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_33","first-page":"127","article-title":"Lycopene Supplementation Enhances Growth Performance, Antioxidant Enzymes, Heat Shock Protein 70, and Some Biochemical and Immune Parameters in Broiler Chickens Exposed to Heat Stress","volume":"13","author":"Gouda","year":"2025","journal-title":"Poult. Sci. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4331\/wjbc.v7.i1.14","article-title":"Complexity of vitamin E metabolism","volume":"7","author":"Birringer","year":"2016","journal-title":"World J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2147\/CEG.S345531","article-title":"Alkaline Phosphatase Pathophysiology with Emphasis on the Seldom-Discussed Role of Defective Elimination in Unexplained Elevations of Serum ALP\u2013A Case Report and Literature Review","volume":"15","author":"Levitt","year":"2022","journal-title":"Clin. Exp. Gastroenterol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1248\/bpb.26.1188","article-title":"Effect of carotenoid on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro: The unique anabolic effect of beta-cryptoxanthin","volume":"26","author":"Yamaguchi","year":"2003","journal-title":"Biol. Pharm. Bull."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"919","DOI":"10.15421\/022158","article-title":"Serum clinical biochemical markers of Hy-Line W-36 laying hens under the influence of increased stocking densities in cages of multilevel batteries","volume":"12","author":"Osadcha","year":"2021","journal-title":"Regul. Mech. Biosyst."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kobaek-Larsen, M., Deding, U., Al-Najami, I., Clausen, B.H., and Christensen, L.P. (2023). Carrot Juice Intake Affects the Cytokine and Chemokine Response in Human Blood after Ex Vivo Lipopolysaccharide-Induced Inflammation. Nutrients, 15.","DOI":"10.3390\/nu15235002"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"19149","DOI":"10.1038\/s41598-025-03450-5","article-title":"Maternal \u03b2-carotene supplementation improves offspring growth, development, immunity, and intestinal microbiota in chickens via immune-mediated and microbial-mediated maternal effects","volume":"15","author":"Wang","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_40","first-page":"364602","article-title":"Haematological and biochemical parameters during the laying period in common pheasant hens housed in enhanced cages","volume":"2014","author":"Chloupek","year":"2014","journal-title":"Sci. World J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.foodchem.2010.03.028","article-title":"Influence of \u03b2-carotene-rich vegetables on the bioaccessibility of zinc and iron from food grains","volume":"122","author":"Gautam","year":"2010","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.nutres.2006.06.015","article-title":"Carotenoids increase iron absorption from cereal-based food in the human","volume":"26","year":"2006","journal-title":"Nutr. Res."},{"key":"ref_43","first-page":"251","article-title":"Physiology of haemoglobin","volume":"12","author":"Thomas","year":"2012","journal-title":"Contin. Educ. Anaesth. Crit. Care Pain"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s40104-019-0402-1","article-title":"Clostridium species as probiotics: Potentials and challenges","volume":"11","author":"Guo","year":"2020","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Kasprzak-Drozd, K., Oniszczuk, T., Stasiak, M., and Oniszczuk, A. (2021). Beneficial Effects of Phenolic Compounds on Gut Microbiota and Metabolic Syndrome. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073715"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3945\/ajcn.110.000075","article-title":"Prebiotic evaluation of cocoa-derived flavanols in healthy humans by using a randomized, controlled, double-blind, crossover intervention study","volume":"93","author":"Tzounis","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6668","DOI":"10.3382\/ps\/pez537","article-title":"Administration of dietary prebiotics improves growth performance and reduces pathogen colonization in broiler chickens","volume":"98","author":"Froebel","year":"2019","journal-title":"Poult. Sci."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/3\/382\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:08:42Z","timestamp":1772208522000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/16\/3\/382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":47,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["life16030382"],"URL":"https:\/\/doi.org\/10.3390\/life16030382","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]}}}