{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:23:08Z","timestamp":1791555788404,"version":"4.3.4"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Background: Acute cholecystitis, a leading cause of urgent surgical intervention, poses challenges in predicting severity and operative complexity. This study characterized the immuno-inflammatory profile distinguishing acute from chronic cholecystitis and assessed whether blood-derived ratios\u2014neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), and platelet-to-lymphocyte (PLR)\u2014correlate with histologic severity and surgical difficulty. Methods: The study retrospectively analyzed 759 patients undergoing cholecystectomy from 2016 to 2024. Inflammatory indices from preoperative bloodwork were compared across histopathologic subtypes (catarrhal, phlegmonous, gangrenous), clinical features, and surgical outcomes, including conversion to open procedure. Logistic regression and ROC analyses identified predictors of acute inflammation and conversion. Results: Acute cholecystitis patients showed elevated NLR (7.0 vs. 3.1), MLR (0.44 vs. 0.26), and PLR (194 vs. 142; all p &lt; 0.001). NLR was the only independent predictor of acute disease (OR = 1.29, 95% CI 1.203\u20131.390, p &lt; 0.001), with superior discrimination (AUC = 0.806, cut-off = 3.56; sensitivity 73.1%, specificity 80.4%). NLR and PLR rose progressively from catarrhal to phlegmonous and gangrenous subtypes (p &lt; 0.05), mirroring conversion rates (0% catarrhal, 3.2% phlegmonous, 10.5% gangrenous; p = 0.001). Conclusions: Routine hematologic ratios capture systemic immune activation in acute cholecystitis, reflecting histologic severity and operative risk. NLR, integrating innate and adaptive immune dynamics, offers a practical biomarker for preoperative risk stratification in acute care surgery.<\/jats:p>","DOI":"10.3390\/life16040565","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T15:20:47Z","timestamp":1774884047000},"page":"565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preoperative Inflammatory Markers (NLR, MLR, PLR) in Evaluating Acute Cholecystitis Severity and Operative Difficulty"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3277-1198","authenticated-orcid":false,"given":"Catalin Vladut Ionut","family":"Feier","sequence":"first","affiliation":[{"name":"Abdominal Surgery and Phlebology Research Center, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, 300041 Timi\u015foara, Romania"},{"name":"First Surgery Clinic, \u201cPius Brinzeu\u201d Clinical Emergency Hospital, 300723 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melania Veronica","family":"Ardelean","sequence":"additional","affiliation":[{"name":"Department V, Internal Medicine I, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, Eftimie Murgu Square 2, 300041 Timi\u015foara, Romania"},{"name":"Center of Advanced Research in Cardiology and Hemostasology, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, Eftimie Murgu Square 2, 300041 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0497-0405","authenticated-orcid":false,"given":"Calin","family":"Muntean","sequence":"additional","affiliation":[{"name":"Medical Informatics and Biostatistics, Department III-Functional Sciences, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, Eftimie Murgu Square No. 2, 300041 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaviana Monique","family":"Faur","sequence":"additional","affiliation":[{"name":"Department of Doctoral Studies, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, 300041 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8647-1003","authenticated-orcid":false,"given":"Vasile","family":"Gaborean","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Research Center, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, Eftimie Murgu Square No. 2, 300041 Timi\u015foara, Romania"},{"name":"Department of Surgical Semiology, Faculty of Medicine, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, Eftimie Murgu Square No. 2, 300041 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3045-6282","authenticated-orcid":false,"given":"Marius Sorin","family":"Murariu","sequence":"additional","affiliation":[{"name":"Abdominal Surgery and Phlebology Research Center, \u201cVictor Babe\u015f\u201d University of Medicine and Pharmacy Timi\u015foara, 300041 Timi\u015foara, Romania"},{"name":"First Surgery Clinic, \u201cPius Brinzeu\u201d Clinical Emergency Hospital, 300723 Timi\u015foara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1053\/j.gastro.2009.02.038","article-title":"Burden of digestive diseases in the United States Part III: Liver, biliary tract, and pancreas","volume":"136","author":"Everhart","year":"2009","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"European Association for the Study of the Liver (EASL) (2016). 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